Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,293,7810GA98.8% 281.8 / NA 80G80D (GGC→GAT) nrfGputative formate‑dependent nitrite reductase complex subunit NrfG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (27/52);  minor base C (1/0);  total (28/52)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,293,7820CT98.8% 264.1 / NA 80G80D (GGC→GAT) nrfGputative formate‑dependent nitrite reductase complex subunit NrfG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/51);  minor base G (0/1);  total (28/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTC  >  NC_000913/4293489‑4294066
                                                                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                                                            
aGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTTGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTAc                                                                                                                                                                                                                                                                                        <  2:343425/300‑1 (MQ=255)
aGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGNAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATACGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTAc                                                                                                                                                                                                                                                                                        <  1:126963/300‑1 (MQ=255)
   ggtgTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCANACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTAt                                                                                                                                                                                                                                                                                     >  2:455226/1‑300 (MQ=255)
    aggttgttACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATc                                                                                                                                                                                                                                                                                    <  2:575234/298‑1 (MQ=255)
     tgttgtTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGTGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:5986/300‑1 (MQ=255)
     tgttgtTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  1:95287/300‑1 (MQ=255)
     tgttgtTACTGCTGAGTGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAACGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  1:95063/300‑1 (MQ=255)
      gttgttACTGCTGATTGCACTGATCCTCTGTCGAGTGAGGGCGAATCGATGAAACAGCCCAAAATACCAGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGGTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGTAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:325440/299‑1 (MQ=255)
       ttgctACTGCTGATTGCACGGATCCTCTGGCGAGTGAGGGCGAAGCGATTAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTAt                                                                                                                                                                                                                                                                                     <  2:103685/292‑1 (MQ=255)
        tgttACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGTTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTAt                                                                                                                                                                                                                                                                                     >  1:324604/1‑295 (MQ=255)
                 tgATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGa                                                                                                                                                                                                                                                                       >  1:155233/1‑300 (MQ=255)
                 tgATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTCCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGa                                                                                                                                                                                                                                                                       >  1:453577/1‑300 (MQ=255)
                        aCTGATCCTCTGGCGAGTGAGTGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAAGGTTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:213717/281‑1 (MQ=255)
                        aCTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATNTTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAg                                                                                                                                                                                                                                                                 <  1:122275/299‑1 (MQ=255)
                        aCTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  1:193200/281‑1 (MQ=255)
                        aCTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAg                                                                                                                                                                                                                                                                 <  2:388408/299‑1 (MQ=255)
                             tccttgggaggcggggggCGAAGCGATGAAACAGCCCAAAAGACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  1:193462/261‑1 (MQ=255)
                                tctGGCGAGTGAGGGCNAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGNGTACTATCTGTGGCAAAANGATTACAGCAATTCGtt                                                                                                                                                                                                                                                        >  1:484348/1‑300 (MQ=255)
                                tctGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGTATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACCGTGGGCGTTACTGGCTGAGTACTATCTGTGGCACAACGATTa                                                                                                                                                                                                                                                                    >  2:215848/1‑288 (MQ=255)
                                      ggcggAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  1:193463/263‑1 (MQ=255)
                                                gAAGCGATGGAACAGCACAAAGTACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATTTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGGCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGAGAAAATCCGCACTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:325641/257‑1 (MQ=255)
                                                  aGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGTAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGc                                                                                                                                                                                                                                      <  1:291345/300‑1 (MQ=255)
                                                                   aaaTACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACgtggtg                                                                                                                                                                                                                     <  1:500943/300‑1 (MQ=255)
                                                                            ttaaaaTTCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGTCAGTTAGCTGTTAAGCCCAAAATGGCAGGCGGGACGTGCGGGGTATCGGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCAGTGCAAGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGAGGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:325853/227‑1 (MQ=255)
                                                                               aaatgcggaaaacacccacaaccttGATAGTACTTTTCTGTGTCGGCAGTTAGTTTTTAAGCCCAAAATGGCAGGCGGTGCGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAACAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTTTGGCAAAACGATTATAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAAc                                                                                                                                                                                                         <  2:238666/277‑1 (MQ=255)
                                                                               aaaTGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGTAAGCGCAGCTGCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGGACCGTCAGGCGTTGCAACGACGTGGTGAGAACGCTGAAc                                                                                                                                                                                                         <  1:306219/300‑1 (MQ=255)
                                                                               aaaTGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAAc                                                                                                                                                                                                         <  2:238677/300‑1 (MQ=255)
                                                                                aaTGCTGACAACACTCACGATATGGATGGTATTTCTCTGTGTCTGCAGTTATCTGTTAAGCCCAAAATTGCATGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCACGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTTTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACTTGGTGAGAACGCTGAAc                                                                                                                                                                                                         <  1:306758/299‑1 (MQ=255)
                                                                                  tGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGaa                                                                                                                                                                                                          >  1:166891/1‑296 (MQ=255)
                                                                                      gACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATgc                                                                                                                                                                                                  <  1:360564/300‑1 (MQ=255)
                                                                                        caacaCTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTAAGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAAAGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATgc                                                                                                                                                                                                  >  2:134164/1‑298 (MQ=255)
                                                                                             cTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCt                                                                                                                                                                                            >  2:570928/1‑299 (MQ=255)
                                                                                                  gATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGAc                                                                                                                                                                                      <  1:242515/300‑1 (MQ=255)
                                                                                                     atTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGt                                                                                                                                                                                   >  2:139254/1‑300 (MQ=255)
                                                                                                                ttCTCTGTGTCGGCAGTGATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTAcc                                                                                                                                                                        <  2:409977/300‑1 (MQ=255)
                                                                                                                 tctcTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACANCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCa                                                                                                                                                                       >  2:458151/1‑300 (MQ=255)
                                                                                                                      gtgtCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTAAATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGCTACTGGATGAGTACTATCTGTGGCAAAAAGATTACAGCAATTCGTTGCTGGTGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGATGAACTGTATGCGGCGATGGCGACGGTACTTTATTACCAGgcc                                                                                                                                                                   >  2:318605/1‑299 (MQ=255)
                                                                                                                        gtCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGgccagc                                                                                                                                                                >  1:54760/1‑300 (MQ=255)
                                                                                                                           ggCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGgccagcca                                                                                                                                                              <  1:577667/299‑1 (MQ=255)
                                                                                                                               gTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCata                                                                                                                                                         >  1:481477/1‑300 (MQ=255)
                                                                                                                                   tCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCGGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACTTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAc                                                                                                                                                     <  1:482128/300‑1 (MQ=255)
                                                                                                                                   tCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAc                                                                                                                                                     <  1:482021/300‑1 (MQ=255)
                                                                                                                                        tAAGCCCAAAATGGCAGTCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGTAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGccc                                                                                                                                                <  2:233090/300‑1 (MQ=255)
                                                                                                                                        tAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGccc                                                                                                                                                <  1:101736/300‑1 (MQ=255)
                                                                                                                                        tAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGCCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGccc                                                                                                                                                <  1:102644/300‑1 (MQ=255)
                                                                                                                                        gcaGCCCAAAACGGCAGGCGGTATGTGCGGAGTATCAGCGTCAGCGCGATTCGCTACATCAGGTTGCCAGCCAGCAAACCACGGAAGCGAAGCTTAAGGCAGTGCATGATAAAATCCGCGCTGGTCCAGAGAACAACAAACAGTGGGCATTACTGAATGAGTACTATCTGTGGCAAAACGATTGCAGCAATTCGTTGCTTGCGTACCGTCAGGCGGTGCAACGACGGGGTGAGAACGCTGAACGGTATGAGGAGCGGGCGAAGGTGCTGTATTACCAGGCCAGCCAGCATATGACCGccc                                                                                                                                                <  2:524484/298‑1 (MQ=255)
                                                                                                                                               aaaaTGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGNACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACt                                                                                                                                           <  2:186521/298‑1 (MQ=255)
                                                                                                                                                            gTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATTAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTANAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGAc                                                                                                                            <  2:417354/300‑1 (MQ=255)
                                                                                                                                                                 tGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGc                                                                                                                       <  1:139254/300‑1 (MQ=255)
                                                                                                                                                                     gAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGTATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTCCCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTc                                                                                                                   >  1:36835/1‑300 (MQ=255)
                                                                                                                                                                     gAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTc                                                                                                                   >  2:372279/1‑300 (MQ=255)
                                                                                                                                                                       gTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcgc                                                                                                                 >  1:533956/1‑300 (MQ=255)
                                                                                                                                                                       gTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAACGCCCAGACTCGCGCAATGATCGACAAAGCCCTcgc                                                                                                                 >  2:29685/1‑300 (MQ=255)
                                                                                                                                                                        tATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcgcg                                                                                                                <  1:54898/300‑1 (MQ=255)
                                                                                                                                                                             gcgtcagcgCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGa                                                                                                           >  1:173516/1‑300 (MQ=255)
                                                                                                                                                                               gtcagcgCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGAc                                                                                                          <  2:116165/299‑1 (MQ=255)
                                                                                                                                                                               gtcagcgCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGAc                                                                                                          <  1:224416/299‑1 (MQ=255)
                                                                                                                                                                                   gcgcGATCCGCTACATCATTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATGGCAGGATAAAATCCGCGCGAATCCACAAAACAGCGAACAGTGGGCGTGACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTaa                                                                                                     <  1:357724/300‑1 (MQ=255)
                                                                                                                                                                                   gcgcGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTaa                                                                                                     <  1:570928/300‑1 (MQ=255)
                                                                                                                                                                                     gcgATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATg                                                                                                   <  1:298578/300‑1 (MQ=255)
                                                                                                                                                                                           cGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCACAGACTCGCGCAATGATCGACAAAGCCCt                                                                                                                    >  2:249071/1‑277 (MQ=255)
                                                                                                                                                                                           cGCTACATCAGTTTGCCAGCCAGCAAACCCAGGAAGCGCAGCTTCAGGTATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGAAAGTAATGAAATc                                                                                              >  2:58023/1‑299 (MQ=255)
                                                                                                                                                                                               aCATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGc                                                                                         >  1:437156/1‑300 (MQ=255)
                                                                                                                                                                                                 aTCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGccc                                                                                       <  1:534170/300‑1 (MQ=255)
                                                                                                                                                                                                                agcAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCt                                                                         <  1:458151/299‑1 (MQ=255)
                                                                                                                                                                                                                      ccccGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCgcgc                                                                                                                                      <  1:351158/232‑1 (MQ=255)
                                                                                                                                                                                                                      ccccGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCgcgc                                                                                                                                      >  2:351158/1‑232 (MQ=255)
                                                                                                                                                                                                                                gcAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGc                                                         <  1:509568/299‑1 (MQ=255)
                                                                                                                                                                                                                                   gCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGTCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGc                                                     >  1:149839/1‑300 (MQ=255)
                                                                                                                                                                                                                                   gCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGACCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGc                                                     >  1:69508/1‑300 (MQ=255)
                                                                                                                                                                                                                                                  ggATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCaa                                        >  2:264524/1‑298 (MQ=255)
                                                                                                                                                                                                                                                   gATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGcc                                     >  1:328976/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             gcgcTAATCCACAAAACAGCGAACAGTGGGCGGTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCATGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACt                             <  1:14160/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aaTCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCACTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGGCAAAGCCCTCGCGCTGGACAGTAATGAGATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAg                                       <  2:49518/283‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aGCGAACATTGGTCGTGACTGGATGAGTACGACCTGTGGCAAAACGACGACAGCAATTCGTTGCGGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCACTGGCGACGGTGCNTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGGCAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATTCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGAt          <  2:191416/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGAt          <  2:377390/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGAt          >  1:356963/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                              aGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCNTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATTCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGAt          <  2:191479/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 gAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGAGTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGAtc         <  2:592870/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      gTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTc  <  1:455226/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                                                            
AGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTC  >  NC_000913/4293489‑4294066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑