Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,333	C→A	H143N (CAC→AAC)	sdhA →	succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,333	0	C	A	100.0%	179.7 / NA	51	H143N (CAC→AAC)	sdhA	succinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/27);  total (24/27)

ATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGA  >  NC_000913/756040‑756616                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                            aTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGc                                                                                                                                                                                                                                                                                         <  1:550042/298‑1 (MQ=255)  tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGAGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTAGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTAGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTCCCGGTAACACACt                                                                                                                                                                                                                                                                                      >  2:282151/1‑300 (MQ=255)         tcggcgcAAGTCTGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCGCGCGTCTCGATGATGGTCGTATCTATCAATGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCATGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGcac                                                                                                                                                                                                                                                                               <  1:232094/297‑1 (MQ=255)         tCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGcac                                                                                                                                                                                                                                                                               <  1:232218/300‑1 (MQ=255)                   gcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATc                                                                                                                                                                                                                                                                     <  1:552312/300‑1 (MQ=255)                      gcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACNTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATcagc                                                                                                                                                                                                                                                                  >  1:115438/1‑300 (MQ=255)                                      gggTAATACCCATGAANATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACNGTTGCACACGCTTTATCANCAGAACCTGAAAAacca                                                                                                                                                                                                                                                  >  1:494476/1‑300 (MQ=255)                                      gggTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacca                                                                                                                                                                                                                                                  >  1:560100/1‑300 (MQ=255)                                                   gAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTtc                                                                                                                                                                                                                                     <  1:437036/300‑1 (MQ=255)                                                                        ctGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGGTTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTCTCTATCACCGTCCGTTTGGTGGTCAGTCGCAAAACTTCGGCGGCGAGCAGGCGGCACGCATTGCGACAGCAGCTGCCCGTCCCGGTAACGCCCTGTTGCCCACGCTTTCTCAGCGGAACCTGCAAAACCACACCAACGTTTTCTCCGAGTGGTATTCGCTGGAt                                                                                                                                                                                                                >  2:398357/2‑300 (MQ=255)                                                                        atGTACGACACCGTGAGAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACGTGaaaaaggcaaccccccgttt                                                                                                                                                                                                                                      >  1:197414/1‑263 (MQ=255)                                                                        atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGAt                                                                                                                                                                                                                >  1:204787/1‑300 (MQ=255)                                                                        atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTACGAGTGGTATGTGCTGGAt                                                                                                                                                                                                                >  1:204795/1‑300 (MQ=255)                                                                        atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTACGAGTGGTATGCGCTGGAt                                                                                                                                                                                                                >  1:204517/1‑300 (MQ=255)                                                                        atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTAGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAAACTGAAAAAACAAACCAACATTGTCTCCGAGTGGTATGCGCTGGAt                                                                                                                                                                                                                >  2:276259/1‑300 (MQ=255)                                                                          gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGACCTCGAACACATGGGCCTGCCGTTCTCGCGTCTCAATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAAACTGAAAAAACACACCACCATTTTCTACGAGTGGTATGCGCTGGATCt                                                                                                                                                                                                              >  1:232616/1‑300 (MQ=255)                                                                                  ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGaaaa                                                                                                                                                                                                      >  1:549002/1‑300 (MQ=255)                                                                                  ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTAGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGGTGCACACGCTTTATCGGCAGAACCTGAAAAACCACACTAACATTTTCTCCGAGTGGTa                                                                                                                                                                                                                          >  2:22300/1‑280 (MQ=255)                                                                                  ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCAGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAAACACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGaaaa                                                                                                                                                                                                      >  2:504601/1‑300 (MQ=255)                                                                                                gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGgcgc                                                                                                                                                                                         >  2:360574/1‑299 (MQ=255)                                                                                                         ggTGACCAGGACGCGAGTGAATATATGTGTAAAACCGGTCCGTAATCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGTTGAAAAACCAGGATGGCGCGGTGGTGGGt                                                                                                                                                                               <  2:504387/300‑1 (MQ=255)                                                                                                          gTGACCAGGGTGCGATTGAGTATATGCGTAAACACGAGCCGGAAGCGATTCTGAAACTCAAACACATGTGCCTGCCGTTCTCGCGTCTCGGTAATGTTCGTATCTATCAACGTCCTTTCGGCGTTCAGTCTAAAAACTTCTGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGt                                                                                                                                                                               <  2:214866/299‑1 (MQ=255)                                                                                                          gTGACCAGGACGCGATTGAATATATGTGTAAAACCGTGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGt                                                                                                                                                                               <  2:549002/299‑1 (MQ=255)                                                                                                                   aCGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCANCTGACCGTACCGGTAACGCACTGTTGCACACGNTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAc                                                                                                                                                                     <  2:436066/300‑1 (MQ=255)                                                                                                                   aCGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAc                                                                                                                                                                     <  1:36459/300‑1 (MQ=255)                                                                                                                           gAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTAGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacacaaaca                                                                                                                                                                                                                                          >  2:149770/1‑218 (MQ=255)                                                                                                                                   gtgtAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAAcc                                                                                                                                                       >  1:69563/1‑298 (MQ=255)                                                                                                                                                  ggAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGTTGAAGGGGTTTAt                                                                                                                                        <  1:447319/298‑1 (MQ=255)                                                                                                                                                                      aaCACATGGGCCTGCCGTTCTCGCGTCTCGATGATGTTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCATACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGc                                                                                                                  <  2:34845/300‑1 (MQ=255)                                                                                                                                                                            tggaccTGCCATTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCTGCGAGCATGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAc                                                                                                                                                                     <  2:166853/239‑1 (MQ=255)                                                                                                                                                                                ccTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTACGTTTGGGGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTACGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAAACGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGTGACt                                                                                                          >  2:275803/1‑298 (MQ=255)                                                                                                                                                                                                    atgatgGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATc                                                                                    <  1:360574/300‑1 (MQ=255)                                                                                                                                                                                                              tatctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTccaccac                                                                          <  1:282151/300‑1 (MQ=255)                                                                                                                                                                                                              tatctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACAAGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTccaccac                                                                          <  1:591997/300‑1 (MQ=255)                                                                                                                                                                                                               atctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTccaccacc                                                                         >  1:476145/1‑300 (MQ=255)                                                                                                                                                                                                                 ctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTAAGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCaa                                                                       >  1:11676/1‑300 (MQ=255)                                                                                                                                                                                                                                ttgCGGTCAGTCTAAAAACTTCGGCGGCGAGCAGGCGGCACGTACTGCGGCAGCAGCTGACCGGACCGGTAACGCATTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTACTGGCGACTGTCGGAGCAGGGCGTATTTATCAGTCCACCACCAACACCCACATTAacac                                                        <  2:168897/298‑1 (MQ=255)                                                                                                                                                                                                                                tGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAacac                                                        <  2:351922/300‑1 (MQ=255)                                                                                                                                                                                                                                         gTCGAAAAACTTCTGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGATTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGt                                                                                                                                                                               <  1:273253/172‑1 (MQ=255)                                                                                                                                                                                                                                         gTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCCGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGt                                                                                                                                                                               >  2:273253/1‑172 (MQ=255)                                                                                                                                                                                                                                         gTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGt                                                                                                                                                                               <  1:280199/172‑1 (MQ=255)                                                                                                                                                                                                                                         gTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGAc                                                 >  1:392892/1‑298 (MQ=255)                                                                                                                                                                                                                                         gTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTACGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGt                                                                                                                                                                               >  2:280199/1‑172 (MQ=255)                                                                                                                                                                                                                                                 aCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCTTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTNGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCa                                       <  2:424494/300‑1 (MQ=255)                                                                                                                                                                                                                                                            gaacatgccgcacgcacTGCGGTAGCAGCCGACCGTACTGTTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCTAGTGGTATGCGCTGGATCTGGGGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCACACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCTCGCGCTACCGTGCTGGCGACTGGCGGAGCGGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCg                             <  2:204795/290‑1 (MQ=255)                                                                                                                                                                                                                                                            gAGCAGGCGGTACACACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCg                             <  2:204517/299‑1 (MQ=255)                                                                                                                                                                                                                                                            gAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCg                             <  2:204787/299‑1 (MQ=255)                                                                                                                                                                                                                                                                       acgcacTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTAcc                 <  2:560100/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          cacTGCGGCAGCAGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTACGAGTGGTATGCGCTGGATCTGGTGAAAAAACAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGTTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACGGGTGACGGTGTCGGCATGGCTATCCGTGCCGGTGTACCGGt              >  2:125780/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gcagcaGCTGACCGTACCGGTAACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGAGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGat       <  1:557944/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      agcTTACCGTACCGGTAACACACTGTTGCACACGCTGTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGGGGTATGCGCTGGATCTGGGGAAAAACCAGGGTGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGGGCCGGCGTACCGGTGCAGGATATGGa  <  2:273977/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                            ATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGA  >  NC_000913/756040‑756616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑