Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,964,254	A→C	V266G (GTC→GGC)	flhA ←	flagellar biosynthesis protein FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,964,254	0	A	C	97.9%	168.5 / NA	48	V266G (GTC→GGC)	flhA	flagellar biosynthesis protein FlhA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (29/18);  minor base T (0/1);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGACCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCAATCAATCCGGCGTTAAGG  >  NC_000913/1963965‑1964541                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                agaATCTTCCAGTTGTATATCGTTCCACTTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCATGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCgcggc                                                                                                                                                                                                                                                                                       <  2:9202/300‑1 (MQ=255)        tCCAGTTGTACGTCGTTCCACGTCGCTTCGATAACGGTATTATTCTCTGCCATTTTTGCCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCATGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCCGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCAGTAGaa                                                                                                                                                                                                                                                                                <  2:242751/300‑1 (MQ=255)                    tCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCCTTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGCATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCACAAATACCAGGTTCGGCATTCAAGGCACGAGGCAGAGTAAACCGAGTACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGCTTCAACATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCACCGCGGCGGTAGAAATCACCa                                                                                                                                                                                                                                                                         >  2:126888/1‑295 (MQ=255)                      gTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAAGCGCTTTTTGTTCGCGTCCGCGTATCCAACAGGCCAGCCCGAGCAATCCGGCAGTGACCAGCAAAAATACCAGNTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCAACATCTGCTCGCCAACATCCTGCTCGGTGCTGACACGTGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAacgc                                                                                                                                                                                                                                                                     >  2:430516/1‑297 (MQ=255)                                          agttattatTCTCTGCCATTTTTATCGGTTTGGGTTCGGCAGGCGCGTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCAGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAACCACCAGCGCCGGAATTTGTGCCACCAGACCg                                                                                                                                                                                                                                               <  2:285128/296‑1 (MQ=255)                                          aggTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCg                                                                                                                                                                                                                                               <  2:283105/298‑1 (MQ=255)                                            gTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGCTGCCCACCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCgct                                                                                                                                                                                                                                             >  1:285128/1‑297 (MQ=255)                                            gTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCgct                                                                                                                                                                                                                                             >  1:283105/1‑297 (MQ=255)                                            gTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTc                                                                                                                                                                                                                                             >  1:343374/1‑299 (MQ=255)                                            gTATTACTCTCTGCCCCTTTTACCAGTTTGGGTTCGGCAGGCGCTTTTTGTTTGCGTCCGCGTACCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGGTTGGCATTCCAGGCACCCGGCCGAGTAAACCGAGCCCGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGTTGATTTACAATCTGCTCGCCAACGTCCTGGTCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAACCACCAGCGCAGGAATTTGTGCCAACAGACCGTc                                                                                                                                                                                                                                             >  2:170680/1‑299 (MQ=255)                                                 attCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGAAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAAt                                                                                                                                                                                                                                       <  1:89891/300‑1 (MQ=255)                                                          cATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATa                                                                                                                                                                                                                               >  1:570505/1‑299 (MQ=255)                                                           aTTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAg                                                                                                                                                                                                                              <  2:488542/299‑1 (MQ=255)                                                             tttcaCCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCTGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCTGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCTTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAg                                                                                                                                                                                                                              <  2:486284/293‑1 (MQ=255)                                                                                           ttGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCAt                                                                                                                                                                                             >  1:554638/1‑300 (MQ=255)                                                                                                 gcgTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATg                                                                                                                                                                                       <  2:533412/300‑1 (MQ=255)                                                                                                    tCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGtt                                                                                                                                                                                     >  1:98868/1‑299 (MQ=255)                                                                                                       gcgTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTgcag                                                                                                                                                                                 <  2:536288/300‑1 (MQ=255)                                                                                                         gTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTNGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTgcagca                                                                                                                                                                               >  1:112221/1‑300 (MQ=255)                                                                                                            tCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGc                                                                                                                                                                            <  1:76155/300‑1 (MQ=255)                                                                                                              caccaGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGcc                                                                                                                                                                           >  1:177432/1‑299 (MQ=255)                                                                                                                       aGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAAc                                                                                                                                                                 >  1:386321/1‑300 (MQ=255)                                                                                                                                 aTCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAGACCGAGCACGGCGGCGCTTAAAAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCAACAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCAATGTTGCAGCACGCAGACCAGCAATCAGCCGACaa                                                                                                                                                       >  2:153656/1‑300 (MQ=255)                                                                                                                                 aTCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACCTAACGCTTGGGTTACTGAAAAGCTGATTCACGATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGAAATTTGTGCAACCAGACCATCGCCAATGGTCAATAGCGTATAAATTTCCGCCGCGTGTCCCATGCTCATACCATGTTGAAGCACGCCGACCCGCAACCCGCCGACaa                                                                                                                                                       >  2:153689/1‑300 (MQ=255)                                                                                                                                           gAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGAc                                                                                                                                             <  2:112221/300‑1 (MQ=255)                                                                                                                                                  aaaaaTACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCatgatg                                                                                                                                      <  2:377834/300‑1 (MQ=255)                                                                                                                                                                    aTTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCa                                                                                                                    >  1:275556/1‑300 (MQ=255)                                                                                                                                                                    aTTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCCGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCa                                                                                                                    >  2:493248/1‑300 (MQ=255)                                                                                                                                                                      tCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATc                                                                                                                  >  2:537142/1‑300 (MQ=255)                                                                                                                                                                      tCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGAACAGCAACCCGCAGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCAt                                                                                                                   >  2:210590/1‑299 (MQ=255)                                                                                                                                                                           gCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATcgccgc                                                                                                             >  1:394451/1‑300 (MQ=255)                                                                                                                                                                                         aaaCCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGNCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAacttactt                                                                                               <  2:180569/300‑1 (MQ=255)                                                                                                                                                                                         aaaCCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATTGCATCGCCGCGAACAAacttactt                                                                                               <  2:590566/300‑1 (MQ=255)                                                                                                                                                                                              gAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACNCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGccc                                                                                           <  2:453913/299‑1 (MQ=255)                                                                                                                                                                                                       ggcgCTTAACAACATAACGCTTGGATTACTGCAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGAACGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCAtt                                                                                   >  2:333645/1‑298 (MQ=255)                                                                                                                                                                                                       ggcgCTTAACAACATAACGCGTGGGTTACTGAAAGGCTGATTCACCATCTGCTCGCCAACAGCCTGATCTGTGCTGACACGCTTGACGATGTCCCCCGCGGCGTTAGAAATCACCAGCGCCGGAATTTGTGCCNCCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCAGGATGAGGATCCCTGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGa                                                                                 <  2:183400/300‑1 (MQ=255)                                                                                                                                                                                                           cttcaccacctcccGCTTGGGTTACTGCAACGCTGATTCCCCCCCTACTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCAACAGCGCCGGAATTTGTGCCACCAGANCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCAATGCTCATGCCATGTTGAAGCACGCCGAACAGCAACCCGCCGACAATGTTAATGAACATGATGAGGATCCCGGCGATGGCATCGACGCGAACAAACTTACTTGCCCCGTCCATTGAGcc                                                                              >  2:421895/14‑299 (MQ=255)                                                                                                                                                                                                                  acaTAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAAt                                                                      >  1:404379/1‑300 (MQ=255)                                                                                                                                                                                                                                  aCTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCAGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGCAAAAATCGACCTCCTGCGTCAc                                                      >  2:104829/1‑300 (MQ=255)                                                                                                                                                                                                                                               ttCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGt                                         >  1:536199/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  cATCCTGATCGGTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACNTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCAc                      >  1:424798/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCCCGCCGCCCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTCACTTGCCCAGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCAATTCGGAGCGGCGTTTTTTCGCCTAATCTTCCCCAATCAATcc            >  2:219537/1‑299 (MQ=255)                                                                                                                                                                                                                                                                             gTGCTGACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCAATCAATcc            >  1:507835/1‑299 (MQ=255)                                                                                                                                                                                                                                                                             gTGCTAACACGCGTAACGATGCCCCCCGCGGCGGTAGAAGTCACCAGCGCCGGAATTTATGCAACCAGACCGTCGCCAATGGTCAATAGCGTATAATTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCCCGCCGACCAGCAACCCGCCGACAATGTTGATGACCATGATGAGGATCCAGGCGATGGCATCGCCGCGAACAAACTTACTTGCACCGTCCa                                                                                     >  2:11954/1‑226 (MQ=255)                                                                                                                                                                                                                                                                             gTGCTAACACGCGTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCAATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCAGGCGATGGCATCGCAGCGAAAAAACTTACTTGCCCAGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGAATCATCTTCACcaatcaat              >  2:98722/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                       gcgcaaCGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGTCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCAATCAATCCGGCGTTAAgg  <  1:201542/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gcgTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCAATCAATCCGGCGTTAAgg  <  1:201702/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gcgTAACGATGCCCCCCGCGGCGGTAGAAATCACCAGCGCCGGAAGTTGTGCCACCAGACCGTCGTCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCAATCAATCCGGCGTTAAgg  <  2:243862/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                AGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTAACAACATAACGCTTGGGTTACTGAAAAGCTGATTCACCATCTGCTCGCCAACATCCTGATCGGTGCTGACACGCGTAACGATGACCCCCGCGGCGGTAGAAATCACCAGCGCCGGAATTTGTGCCACCAGACCGTCGCCAATGGTCAATAGCGTATAACTTTCCGCCGCGTGTCCCATGCTCATGCCATGTTGCAGCACGCCGACCAGCAACCCGCCGACAATGTTAATGACCATGATGAGGATCCCGGCGATGGCATCGCCGCGAACAAACTTACTTGCCCCGTCCATTGAGCCGTAAAAATCGGCTTCCTGAGTCACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCAATCAATCCGGCGTTAAGG  >  NC_000913/1963965‑1964541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑