Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,224,169	A→G	F234F (TTT→TTC)	pbpG ←	peptidoglycan DD‑endopeptidase PbpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,224,169	0	A	G	100.0%	282.8 / NA	80	F234F (TTT→TTC)	pbpG	peptidoglycan DD‑endopeptidase PbpG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/48);  total (32/48)

GGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGG  >  NC_000913/2223875‑2224446                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                      ggAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGcc                                                                                                                                                                                                                                                                                  <  2:605227/300‑1 (MQ=255)           gCAGGAAGTGGAGTAAAAATTACGGATGGCAGAATATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGtt                                                                                                                                                                                                                                                                        <  2:120521/300‑1 (MQ=255)                                                    aTCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAg                                                                                                                                                                                                                                <  1:371598/299‑1 (MQ=255)                                                     tCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCAACGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCCTTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGt                                                                                                                                                                                                                               >  2:382729/1‑299 (MQ=255)                                                        gAATTCACTTAGTCGTTCTTTGCCGTCTGCCCCGCCGCCGCCATTTTGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCGGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATTGCGATACACCAGATGATTAGTAt                                                                                                                                                                                                                             <  2:469141/298‑1 (MQ=255)                                                        gAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTAt                                                                                                                                                                                                                             <  1:409572/298‑1 (MQ=255)                                                        gAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTCTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACTAGATGATTAGTAt                                                                                                                                                                                                                             <  2:290128/298‑1 (MQ=255)                                                           ttCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCgg                                                                                                                                                                                                                        <  2:343384/300‑1 (MQ=255)                                                                    tCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGc                                                                                                                                                                                                               >  1:105859/1‑300 (MQ=255)                                                                          tgtgCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATAc                                                                                                                                                                                                         <  2:432410/300‑1 (MQ=255)                                                                           gttccGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACATGCATCACTTTACCTGTTTCAATCCGGGTACGCGGGCGGCTGGCATCGGCAAAATGGGTATATTTACCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACg                                                                                                                                                                                                        <  2:301952/297‑1 (MQ=255)                                                                           gtgtcttCTGCCCCGCCGCCGCCATTTGTGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATGAGTATTGCGGAACGGCAGCGTATACg                                                                                                                                                                                                        <  2:142729/294‑1 (MQ=255)                                                                           gtgCCTTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCTGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACg                                                                                                                                                                                                        <  2:301799/300‑1 (MQ=255)                                                                           gtgCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTCGTATTGCGGAACGGCAGCGTATAc                                                                                                                                                                                                         >  1:386251/1‑299 (MQ=255)                                                                           gtgCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATAc                                                                                                                                                                                                         >  2:695666/1‑299 (MQ=255)                                                                           gtgCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATAc                                                                                                                                                                                                         >  1:385058/1‑299 (MQ=255)                                                                             gCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTc                                                                                                                                                                                                      <  1:203599/300‑1 (MQ=255)                                                                             gCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTc                                                                                                                                                                                                      <  2:644610/300‑1 (MQ=255)                                                                             gCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTGATAGCGCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCTGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTc                                                                                                                                                                                                      <  2:146622/300‑1 (MQ=255)                                                                               cGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGta                                                                                                                                                                                                      >  1:164102/1‑297 (MQ=255)                                                                               cGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTc                                                                                                                                                                                                      >  1:163501/1‑298 (MQ=255)                                                                                   tGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGAtt                                                                                                                                                                                                 <  1:386823/299‑1 (MQ=255)                                                                                    gCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTa                                                                                                                                                                                                >  2:702921/1‑299 (MQ=255)                                                                                         gccgccgccATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTagag                                                                                                                                                                                             <  2:608009/297‑1 (MQ=255)                                                                                            gccgccATTCGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCNCTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCACGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACAACAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGtt                                                                                                                                                                                       >  2:511430/1‑300 (MQ=255)                                                                                                  aTTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACgctgtctcttatacacatctcgc                                                                                                                                                                                  <  1:364431/299‑23 (MQ=255)                                                                                                  aTTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCa                                                                                                                                                                                   <  1:207094/298‑1 (MQ=255)                                                                                                    ttGGGCGGCTTTTTTTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATc                                                                                                                                                                               <  2:334360/300‑1 (MQ=255)                                                                                                    ttGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCTGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATc                                                                                                                                                                               <  2:480518/300‑1 (MQ=255)                                                                                                    ttGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCGGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATc                                                                                                                                                                               <  2:565999/300‑1 (MQ=255)                                                                                                          ggcTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCg                                                                                                                                                                         <  1:208202/300‑1 (MQ=255)                                                                                                                  gTTTTTTATAGCTCAACNCTGCTGCCGGCACAGGCANCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATNTCTTCCCGGGTAGTAc                                                                                                                                                                 <  1:592154/300‑1 (MQ=255)                                                                                                                  gTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATTGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTAc                                                                                                                                                                 <  1:348758/300‑1 (MQ=255)                                                                                                                  gTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGTTAGTAc                                                                                                                                                                 <  1:348784/300‑1 (MQ=255)                                                                                                                  gTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTAc                                                                                                                                                                 <  2:638038/300‑1 (MQ=255)                                                                                                                  gTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTAc                                                                                                                                                                 <  1:553800/300‑1 (MQ=255)                                                                                                                  gTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCAGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTAc                                                                                                                                                                 >  2:568732/1‑300 (MQ=255)                                                                                                                  gTTTTTGATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCACCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTAc                                                                                                                                                                 <  2:298050/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACTCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGCCCATCACTACCATCGCCACCGGTTTATTATTGATAACCGTACGCATCACCATACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGACTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                            <  2:273555/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCATGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTTGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                            <  2:422136/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                            <  2:423843/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                            <  1:553801/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTGCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                            <  2:138943/300‑1 (MQ=255)                                                                                                                       ttATAGCTCAACGCTGCTGCCGACACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAAc                                                                                                                                                            <  2:389330/300‑1 (MQ=255)                                                                                                                                                  ggCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCt                                                                                                                                  >  2:401559/1‑299 (MQ=255)                                                                                                                                                        aCTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAAt                                                                                                                            >  1:476920/1‑299 (MQ=255)                                                                                                                                                         cTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGa                                                                                                                          >  2:408199/1‑300 (MQ=255)                                                                                                                                                          tttACCGTTTTCAATCCAGGTACGCAGGCGGCTGGCAGCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAANATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAg                                                                                                                         <  2:499818/300‑1 (MQ=255)                                                                                                                                                           ttACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATNTTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGc                                                                                                                        <  2:515676/300‑1 (MQ=255)                                                                                                                                                           ttACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTNTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACAGATATTGTTTGCTGGCAATGAGc                                                                                                                        <  2:553667/300‑1 (MQ=255)                                                                                                                                                                      aaTCCAGGTACGCAGTCGGCTGGCATCGGCAAAATGGGTATATTTGCAAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTa                                                                                                              <  1:573941/299‑1 (MQ=255)                                                                                                                                                                      aaTCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTa                                                                                                              <  2:427153/299‑1 (MQ=255)                                                                                                                                                                      aaTCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTa                                                                                                              <  1:224671/299‑1 (MQ=255)                                                                                                                                                                      aaTCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACCGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTa                                                                                                              <  2:695662/299‑1 (MQ=255)                                                                                                                                                                          cAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCa                                                                                                         >  2:106540/1‑300 (MQ=255)                                                                                                                                                                          cAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACAGCAGCGTATACGTCGGATTCGAGAAGGTTGCCATATCTTCCAGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGTAGTTTGGTTAAGTCa                                                                                                         >  2:104927/1‑300 (MQ=255)                                                                                                                                                                           aGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCaa                                                                                                        >  2:123065/1‑299 (MQ=255)                                                                                                                                                                                          tGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACg                                                                                          <  1:254698/299‑1 (MQ=255)                                                                                                                                                                                                 ggCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCAc                                                                                  <  1:8374/300‑1 (MQ=255)                                                                                                                                                                                                              tctttGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTa                                                                     >  1:633292/3‑300 (MQ=255)                                                                                                                                                                                                                      aaaCGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGGTcaac                                                             <  1:682382/300‑1 (MQ=255)                                                                                                                                                                                                                        aCGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTcaacaa                                                           <  1:453309/300‑1 (MQ=255)                                                                                                                                                                                                                           cgTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAgc                                                        >  1:624902/1‑300 (MQ=255)                                                                                                                                                                                                                            gTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTc                                                                                                                                                                                                      >  2:424880/1‑157 (MQ=255)                                                                                                                                                                                                                            gTCCATCACTACCAGCGCCAACGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTc                                                                                                                                                                                                      >  2:44112/1‑157 (MQ=255)                                                                                                                                                                                                                                    cTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGtgttgt                                               >  2:409768/1‑300 (MQ=255)                                                                                                                                                                                                                                          gcgcCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGTCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTc                                         <  2:636923/300‑1 (MQ=255)                                                                                                                                                                                                                                          gcgcCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTc                                         <  1:617296/300‑1 (MQ=255)                                                                                                                                                                                                                                             ccACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGa                                      >  1:649299/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ttattaTTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAATGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCAAtttc                              >  2:373631/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ttattaTTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttc                              >  2:373560/1‑300 (MQ=255)                                                                                                                                                                                                                                                          attGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTNTTGCGGAACGGCAGCGTATACGTCGGATTAGANAAGGTTGCCATATCTTCCCGGGTAGTACTTCACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAACGCGTGTTGTTCATTCCGAGCGAtttcgcttt                         >  2:518509/1‑300 (MQ=255)                                                                                                                                                                                                                                                          attGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTNTTGCGGAACGGCAGCGTATACGTCGGATTAGANAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTCTGCACCGACAATCCGGTAGGTTCAACAAAGAGCGTGTTGTTCATTCCGAGCGAtttcgcttt                         >  2:518955/1‑300 (MQ=255)                                                                                                                                                                                                                                                          attGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttcgcttt                         >  1:265803/1‑300 (MQ=255)                                                                                                                                                                                                                                                           ttGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCgatttcgatttc                        >  2:436000/1‑300 (MQ=255)                                                                                                                                                                                                                                                            tGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttcgctttc                        >  1:187069/1‑299 (MQ=255)                                                                                                                                                                                                                                                                 aCCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGcattc                  >  1:262434/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 aCCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGcattc                  >  2:70968/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             aCCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCNGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTtaata      >  2:507919/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gACAATGGCCCGCCGCATTGTTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAgg  <  1:172817/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                      GGAAAAACTGGCAGGAAGTGGAGTAAAAATTACGGATGGCAGAGTATCGCCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGG  >  NC_000913/2223875‑2224446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑