Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,956,003	G→A	D225N (GAT→AAT)	ilvA →	threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,956,003	0	G	A	100.0%	184.1 / NA	55	D225N (GAT→AAT)	ilvA	threonine deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/25);  total (30/25)

CTGCTCCACGGCGCGAACTTTGATGAAGCGAAAGCCAAAGCGATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAACTTCCACGG  >  NC_000913/3955715‑3956298                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                        ctgctCCACGGCGCGAACTTTGATGAAGCGAAAGCCAAAGCGATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCAt                                                                                                                                                                                                                                                                                              >  1:687897/1‑300 (MQ=255)   gctCCACGGCGCGAACTTTGATGAAGCGAAAGCCAAAGCGATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCNGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCAGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATcc                                                                                                                                                                                                                                                                                            >  2:551177/1‑300 (MQ=255)                                aaGCCAAAGCGATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCCGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAANTGATCGCCGTAGAAGCGGAAGACTCAGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTAttt                                                                                                                                                                                                                                                                >  2:549858/1‑299 (MQ=255)                                 aGCCAAAGCGATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGAATATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCACACCTAGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTATGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGCTGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGACTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGc                                                                                                                                                                                                                                                              >  2:152916/1‑300 (MQ=255)                                  gCCAAAGCGATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCAGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGAATCCGCCTGCATGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCt                                                                                                                                                                                                                                                             >  2:439597/1‑300 (MQ=255)                                        gCGATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGc                                                                                                                                                                                                                                                       <  2:608398/300‑1 (MQ=255)                                               aaCTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGc                                                                                                                                                                                                                                                  >  1:272404/1‑298 (MQ=255)                                                    tCACAGCAGCAGGGTTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACGGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTaaaa                                                                                                                                                                                                                                           <  2:123665/300‑1 (MQ=255)                                                            gcagGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGAATGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCATGAAAGCAGCGCTGAATGCGGGTCGTCCGGTTGATCTGCCGCGCGTAAGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATc                                                                                                                                                                                                                                     >  2:96348/1‑298 (MQ=255)                                                               ggggTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGTGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCAGTAGAAGCGGAAGACTCCGCATGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGATGAAGGCGTAACGGTAAAACGCATAGGTGa                                                                                                                                                                                                                                >  2:285902/1‑300 (MQ=255)                                                               ggggTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGa                                                                                                                                                                                                                                >  2:683552/1‑300 (MQ=255)                                                                gggTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATc                                                                                                                                                                                                                                                                                    >  1:439718/1‑247 (MQ=255)                                                                gggTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATc                                                                                                                                                                                                                                                                                    <  2:63977/247‑1 (MQ=255)                                                                gggTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATc                                                                                                                                                                                                                                                                                    <  2:439718/247‑1 (MQ=255)                                                                gggTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATc                                                                                                                                                                                                                                                                                    >  1:63977/1‑247 (MQ=255)                                                                     cACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAAc                                                                                                                                                                                                                          >  2:619829/1‑300 (MQ=255)                                                                      aCCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAATGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAAc                                                                                                                                                                                                                          <  1:415521/299‑1 (MQ=255)                                                                                            aTCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTAtc                                                                                                                                                                                                   >  1:94806/1‑300 (MQ=255)                                                                                                             gggCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCAc                                                                                                                                                                                   <  1:181415/299‑1 (MQ=255)                                                                                                             gggCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCAc                                                                                                                                                                                   <  2:459824/299‑1 (MQ=255)                                                                                                             gggCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTAGACGACATCATCAcc                                                                                                                                                                                  >  1:4604/1‑300 (MQ=255)                                                                                                              ggCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTAGACGACATCATCAcc                                                                                                                                                                                  >  2:671851/1‑299 (MQ=255)                                                                                                               gCAAGGCACGCTTGCGTTGGAACTGCTCCAGCAGGACGCCCATCTCGACCTCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCGGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCAc                                                                                                                                                                                   <  2:459481/297‑1 (MQ=255)                                                                                                                   ggCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGNCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGAt                                                                                                                                                                            <  1:140487/300‑1 (MQ=255)                                                                                                                   ggCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGAt                                                                                                                                                                            <  1:688086/300‑1 (MQ=255)                                                                                                                    gCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTTATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGTTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGAt                                                                                                                                                                            <  2:257204/299‑1 (MQ=255)                                                                                                                      aCGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgc                                                                                                                                                                         <  1:457713/300‑1 (MQ=255)                                                                                                                             cgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgcgatgcga                                                                                                                                                                  >  1:186593/1‑300 (MQ=255)                                                                                                                             cgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTACTTGCTGAAGGCGTAGCGGTAAAACGCACCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgtgatgc                                                                                                                                                                    >  2:141983/1‑298 (MQ=255)                                                                                                                                 ggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGANTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCAAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCt                                                                                                                                                               >  2:505130/1‑299 (MQ=255)                                                                                                                                               agGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAg                                                                                                                                                 <  1:289714/299‑1 (MQ=255)                                                                                                                                                   cGCCCATCTAGAACGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCTTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCAACGTCGATAGTGATGCGATCTGTGCGGCGATGAGGGAttt                                                                                                                                            >  2:67318/1‑300 (MQ=255)                                                                                                                                                       cATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTc                                                                                                                                        >  2:658928/1‑300 (MQ=255)                                                                                                                                                                    tATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAAACTTCCGTTTATGCCAGGAGTATCTAGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGACGGATTTATTCGAAGATGTgc                                                                                                                              >  2:23348/1‑297 (MQ=255)                                                                                                                                                                        tgtgCTAGTCGGCGGCGGCAGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTACCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGt                                                                                                                       <  2:121786/300‑1 (MQ=255)                                                                                                                                                                               gTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGaa                                                                                                                <  1:266986/300‑1 (MQ=255)                                                                                                                                                                                      gcggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCNTCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCctct                                                                                                          >  1:515359/1‑299 (MQ=255)                                                                                                                                                                                         gcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGgcg                                                                                                      <  1:696056/300‑1 (MQ=255)                                                                                                                                                                                                                     gATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAata                                                                          >  1:295043/1‑300 (MQ=255)                                                                                                                                                                                                                     gATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTAGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAata                                                                          >  1:636283/1‑300 (MQ=255)                                                                                                                                                                                                                                  aTGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGcac                                                             <  2:654000/300‑1 (MQ=255)                                                                                                                                                                                                                                       gCAAATTAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCATGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCacaac                                                          <  2:295043/298‑1 (MQ=255)                                                                                                                                                                                                                                       gCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGTTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCacaac                                                          <  1:215515/298‑1 (MQ=255)                                                                                                                                                                                                                                       gCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCacaac                                                          <  2:636283/298‑1 (MQ=255)                                                                                                                                                                                                                                       gCAAATCAAAGTGATCGCCGGAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCacaac                                                          <  1:564063/298‑1 (MQ=255)                                                                                                                                                                                                                                          aaTCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTAGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTc                                                      >  1:80050/1‑299 (MQ=255)                                                                                                                                                                                                                                                   gATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTACCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACg                                            <  2:651655/300‑1 (MQ=255)                                                                                                                                                                                                                                                       gCCGTATAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGc                                          <  2:196870/298‑1 (MQ=255)                                                                                                                                                                                                                                                        ccGTAGAAGCGGAAGACTCCGCCTACCTGAAAGCAGCGCTGAATGCGGATCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTCTTCGAAGATGTGCGCGCGGTGTCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAACATATCGCCCTGCACAACATTCGCGGCGAACggtt                                         >  2:46932/1‑296 (MQ=255)                                                                                                                                                                                                                                                             gAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCat                                  >  1:578668/1‑300 (MQ=255)                                                                                                                                                                                                                                                               aGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAAGGGCTGGCGCatat                                <  1:549858/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cctgAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCAAACGGCTGGCGCATATTCTTTCCGGTGCCAACGt              >  1:436172/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           gcgcTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAACTTCCAc    >  2:394328/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             gcTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAACTTCCACgg  <  1:667554/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gcTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCAGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGANAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAACTTCCAc    >  1:132018/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                        CTGCTCCACGGCGCGAACTTTGATGAAGCGAAAGCCAAAGCGATCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTTGCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCGGCGAACGGCTGGCGCATATTCTTTCCGGTGCCAACGTGAACTTCCACGG  >  NC_000913/3955715‑3956298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑