Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,729,510	A→G	K142E (AAA→GAA)	lhr →	ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,729,510	0	A	G	100.0%	210.8 / NA	62	K142E (AAA→GAA)	lhr	ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/35);  total (27/35)

GGTGATTGCACCGACCGGCTCCGGGAAAACGCTGGCAGCATTTCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCC  >  NC_000913/1729230‑1729799                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                  ggTGATTGCACCGACCGGCTCCGGGAAAACGCTGGCAGCATTTCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATccgc                                                                                                                                                                                                                                                                                 >  2:332446/1‑299 (MQ=255)   tGATTGCACCGACCGGCTCCGGGAAAACGCTGGCAGCATTTCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATccgcc                                                                                                                                                                                                                                                                                >  1:474687/1‑298 (MQ=255)                    tcctggAAAACGCTGGCAGCATTTCGCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTTAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAACCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATTCGCCGGATATTCTGATcacc                                                                                                                                                                                                                                                                <  2:179411/293‑1 (MQ=255)                           aaaCGCTGGCAGCATTTCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAAt                                                                                                                                                                                                                                                      <  2:579034/300‑1 (MQ=255)                           aaaCGCTGGCAGCATTTCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAAt                                                                                                                                                                                                                                                      <  1:331226/300‑1 (MQ=255)                                     agcaTTTCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCt                                                                                                                                                                                                                                            <  2:18122/300‑1 (MQ=255)                                     agcaTTTCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCt                                                                                                                                                                                                                                            <  1:455500/300‑1 (MQ=255)                                     agcaTTTCTCTACGCCCTCGATCGGCTCTGCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCt                                                                                                                                                                                                                                            <  2:623675/300‑1 (MQ=255)                                         tttCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACACATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGAt                                                                                                                                                                                                                                         >  1:338992/1‑299 (MQ=255)                                          ttCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAGCCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCANATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCTGCGAAACGGAAGTCAATCTTCGCTTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGc                                                                                                                                                                                                                                       <  2:208059/300‑1 (MQ=255)                                          ttCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCTTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGc                                                                                                                                                                                                                                       <  1:473006/300‑1 (MQ=255)                                          ttCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCTTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGc                                                                                                                                                                                                                                       <  2:118760/300‑1 (MQ=255)                                          ttCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGc                                                                                                                                                                                                                                       <  2:640967/300‑1 (MQ=255)                                          ttCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGc                                                                                                                                                                                                                                       <  1:473014/300‑1 (MQ=255)                                           tctcTACGCCCTCGCTCGGCTCTTACGCGAAGGCGGCGAAGCTACCCGCGAGGCGCATAAGCGTAAAACCTAACGCATCCTATATATTTCAACGATAAAAGCCCTGGGCACCGGAGTTCAGCGCAACTTGCAGATCCAGTTGAAGGGTATTGCCGATGAACGGCGGAGGCGCTGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCAGGCATGGGGAAGCAGCGAACTCACCCGTAATCTGCAGGGTATTCTGATCACAAAGCCCGCATCGCTATATCTGATGc                                                                                                                                                                                                                                       >  2:346780/1‑299 (MQ=255)                                           tctcTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCt                                                                                                                                                                                                                                      >  1:337435/1‑300 (MQ=255)                                           tctcTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTAACCGATAAAAGCACTGGGCACCGACGTTCAGCGCAACTTGCAGATCCAGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTAGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAGTCCGCCGGATATTCTGATCACCACACCCGAATCAACCTATCTGATGCt                                                                                                                                                                                                                                      >  2:346797/1‑300 (MQ=255)                                            ctctACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTAACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGGCGTTCAGCGCAACTTGCAGATCAAGTTGAAGGGTATTGCAGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCAGCCGGATATTCTGATCGCCACACCCGAATCCCTCTATCTgctgct                                                                                                                                                                                                                                      >  2:236673/1‑299 (MQ=255)                                               tACGCCCTCGATCGGCNCTTCCGCGAAGGCGGCGANGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTTAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCTTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCANTCTATCTGATGCTGAc                                                                                                                                                                                                                                   <  1:594284/299‑1 (MQ=255)                                                   cccTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGCACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGCATCACTCTATCTGATGc                                                                                                                                                                                                                                       >  2:241762/1‑291 (MQ=255)                                                   cccTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTccc                                                                                                                                                                                                                              >  2:630273/1‑300 (MQ=255)                                                   cccTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTccc                                                                                                                                                                                                                              >  1:680716/1‑300 (MQ=255)                                                   cccTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATAAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGTATCCGTACTGGCGATACGCATGCCCAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCCATCTCTCTGATTCTgc                                                                                                                                                                                                                                    >  1:680704/1‑293 (MQ=255)                                                                 ttCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCGCTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACg                                                                                                                                                                                                                <  1:482213/300‑1 (MQ=255)                                                                       gAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAcgc                                                                                                                                                                                                          <  1:76966/300‑1 (MQ=255)                                                                              gcgAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCAACGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATTACTCTATCTGATGCTGACCTCCAGCGCGCGCGAAACGCTACGCGACGTc                                                                                                                                                                                                    >  2:47506/1‑299 (MQ=255)                                                                                               gcgcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCATACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGCTCACCACACCCGAATCACTCTATCTGATACTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgat                                                                                                                                                                                  >  1:672666/1‑300 (MQ=255)                                                                                                          tAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAcgc                                                                                                                                                                       <  1:564643/300‑1 (MQ=255)                                                                                                                    cgcctCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTNATGCTGACCTCCAGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGc                                                                                                                                                             >  1:143606/5‑300 (MQ=255)                                                                                                                    cGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTNATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGc                                                                                                                                                             >  1:144499/1‑300 (MQ=255)                                                                                                                    cGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCAGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTNATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGc                                                                                                                                                             >  1:145465/1‑300 (MQ=255)                                                                                                                       aTCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTGGCAGATCCCGTTGAAGGGTATTGCTGATGAGCGGCGGCTGCGCGGCGAAACGGAAGGCAATCTTCGCGTAGGGATCCNTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAACCACTCTATCTGATGCCGACCCCCCGCGCGCGCTAAACACTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACACGGTAGCGGGc                                                                                                                                                             <  2:223743/297‑1 (MQ=255)                                                                                                                         ccTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaa                                                                                                                                                        >  2:571009/1‑300 (MQ=255)                                                                                                                          ctctATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaaa                                                                                                                                                       >  1:609462/1‑300 (MQ=255)                                                                                                                          ctctATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGAATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAGCGGTAATTATTGATAAAGTCCACGCGGTAGCGGGCAGTaaa                                                                                                                                                       >  1:409473/1‑300 (MQ=255)                                                                                                                             tataTTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACg                                                                                                                                                     <  1:6903/299‑1 (MQ=255)                                                                                                                             tataCTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGTGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACg                                                                                                                                                     <  1:7391/299‑1 (MQ=255)                                                                                                                               tatTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGTCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtgg                                                                                                                                                  <  1:200969/300‑1 (MQ=255)                                                                                                                                                    gggCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCt                                                                                                                             >  1:616469/1‑300 (MQ=255)                                                                                                                                                                     gcAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgcgc                                                                                                            <  1:44397/300‑1 (MQ=255)                                                                                                                                                                       aaCTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGAGGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgcgc                                                                                                            <  1:44398/298‑1 (MQ=255)                                                                                                                                                                               gATCCCTTTGAAGGTTATTGCCGATAAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGTCGATACGCCTGCACATGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGACCACCACACCCGAATCACTCTATCTGATGCTGACCCCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCacac                                                                                                  <  2:156915/300‑1 (MQ=255)                                                                                                                                                                                aTCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTACCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACAcc                                                                                                 <  1:410129/300‑1 (MQ=255)                                                                                                                                                                                   ccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGANCTCCCGCGTGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGACGTACACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGCCTGGAGCGGCTCGATGCGCTGCTCCACACCTCa                                                                                              >  2:212623/1‑300 (MQ=255)                                                                                                                                                                                      tttaaGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTTTCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGGAGTCCACGCGGTAGCGTGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCacac                                                                                                  <  2:156094/290‑1 (MQ=255)                                                                                                                                                                                       tGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGcac                                                                                          <  1:559999/300‑1 (MQ=255)                                                                                                                                                                                       tGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGcac                                                                                          <  2:609462/300‑1 (MQ=255)                                                                                                                                                                                             gTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTAGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAAGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGaa                                                                                    >  2:84292/1‑300 (MQ=255)                                                                                                                                                                                                             gcggcggcgCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCAc                                                                    <  1:452353/300‑1 (MQ=255)                                                                                                                                                                                                             gcggcggcgCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACACTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAGTTGGCTTTTCTGCCAc                                                                    <  1:452358/300‑1 (MQ=255)                                                                                                                                                                                                                                gAAGTCAATCTTCGCGGAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCg                                                   <  2:120603/298‑1 (MQ=255)                                                                                                                                                                                                                                gAAGTCAACCTGCGCTTAGGGATCCGTACTGTCGATACGCCTACACAGGAACGCAGCGAACTCACCCGGAATCCTCCGGAGATTCTGATCACCACACCCAAATCATGCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACCCGGCTTCGAAACGTTAATTATTGATGAAGTCCACGCGGTAGCGGGCATTAAACGTGGTGCGTATCTGGCGTTAAGTCTGGGGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTTCCACTGTGCGCTcagccctcgac                                                 <  2:373488/300‑7 (MQ=255)                                                                                                                                                                                                                                 aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGTTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGTATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGAt                                                 <  2:373255/299‑1 (MQ=255)                                                                                                                                                                                                                                   gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCAACTGTGCGCTCAGCCAGCGAtgt                                               >  2:428586/1‑299 (MQ=255)                                                                                                                                                                                                                                                                cGATACGCCTGCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAAGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTNAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGt                 >  1:138035/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gCACAGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTc       >  1:663446/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           caccggAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATTAATCTATCTGATGCTGACCTCCCGCGCGCGCGAAGCGCTAAGCGGCGTAGAAACGGTAATTATTGATGAAGTCCCCGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCCGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCCTGGTGGCGATCGCCCGGTTACGGTa          >  2:421727/5‑296 (MQ=255)                                                                                                                                                                                                                                                                           cacaGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAAGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTAAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCa      >  2:421705/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aGGAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAAc    <  2:699585/299‑1 (MQ=255)                                                                                                                                                                                                                                                                              aGGAACGCAGCGAACTCACCCGTAACCAGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAAc    <  2:567784/299‑1 (MQ=255)                                                                                                                                                                                                                                                                               ggAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAAccc  <  2:446341/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gAACGCAGCGAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCCGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAAccc  <  2:474687/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                  GGTGATTGCACCGACCGGCTCCGGGAAAACGCTGGCAGCATTTCTCTACGCCCTCGATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCC  >  NC_000913/1729230‑1729799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑