Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,816,316	C→T	A78A (GCG→GCA)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,816,316	0	C	T	100.0%	217.5 / NA	63	A78A (GCG→GCA)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/27);  total (36/27)

TCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCGGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGT  >  NC_000913/3816048‑3816606                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                   ttacaATACCGACAGAAACCGCGGCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAAc                                                                                                                                                                                                                                                                     <  2:441972/298‑1 (MQ=255)                        gCTACCATCCCTTTCATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGc                                                                                                                                                                                                                                               >  1:401979/1‑299 (MQ=255)                          tACCATCCCTTTTATTGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTTAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGc                                                                                                                                                                                                                                               <  2:197517/297‑1 (MQ=255)                                    ttCATTGGATTTGTTTTCAGCTTGCCTTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTTAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCACTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAgcc                                                                                                                                                                                                                                   <  2:348675/299‑1 (MQ=255)                                    ttCATTGGATTGGTTTTCAGCTTGCCGTGTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGTGCGATCAGACGCTTGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGc                                                                                                                                                                                                                                               <  2:198735/287‑1 (MQ=255)                                         tggATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTTCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAgccctggcc                                                                                                                                                                                                                             <  2:646295/299‑1 (MQ=255)                                              tGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGAc                                                                                                                                                                                                                        <  1:628907/300‑1 (MQ=255)                                               ggTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGAcc                                                                                                                                                                                                                       >  1:638481/1‑300 (MQ=255)                                                                 ttCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCAc                                                                                                                                                                                                     <  2:704601/300‑1 (MQ=255)                                                                        aGCTTCTGTAGCGCATCTGCCAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCcttct                                                                                                                                                                                              >  2:613797/1‑300 (MQ=255)                                                                                         tgccAGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTaca                                                                                                                                                                             >  2:637835/3‑300 (MQ=255)                                                                                             aGCGCCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACacaat                                                                                                                                                                         <  1:322214/300‑2 (MQ=255)                                                                                              gcgcCACACAGGCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGNACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACacaata                                                                                                                                                                        >  1:510947/1‑298 (MQ=255)                                                                                                   acacAGGCACCCGTAATCTAAGCGGTACTCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTGCTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGGGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGTAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACttt                                                                                                                                                                    <  2:311716/296‑1 (MQ=255)                                                                                                   acacAGGCACCCGGAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCACTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACttt                                                                                                                                                                    <  2:311792/296‑1 (MQ=255)                                                                                                        ggCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGTGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGAGGTGATCCAGCCCTGGCACTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGCGGCGGTACACAATACTTTGGTATc                                                                                                                                                              >  2:272491/1‑300 (MQ=255)                                                                                                        ggCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACCCTGCGGTGATCCAGCACTGGCCATGAACTTTCAGGAAGCGAGGCACGCCTTCTTCAATAAAGGCGGTACACAATACTTTGGTATc                                                                                                                                                              >  2:273734/1‑300 (MQ=255)                                                                                                        ggCACCCGTAATCGAAGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTAGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCACTGAACTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTCCACAATACTTTGGt                                                                                                                                                                 >  2:273756/1‑297 (MQ=255)                                                                                                                      aagcgGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGTGGTACACAATACTTTGGTATCGCAAAATCCGaaca                                                                                                                                                >  2:386457/3‑297 (MQ=255)                                                                                                                             aCGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTNCCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGa                                                                                                                                         >  2:516385/1‑300 (MQ=255)                                                                                                                              cgcgTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGa                                                                                                                                         >  1:419322/1‑299 (MQ=255)                                                                                                                               gcgTGCCACCATCAGCCTGAAGCACTTCGCAGTCCAGCTTAATGTTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGTAAGCTAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGc                                                                                                                                       <  2:34525/300‑1 (MQ=255)                                                                                                                               gcgTGCCACCATCAGCCTGAAGCACTTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCGCCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGNGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTTGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGc                                                                                                                                       <  2:517944/300‑1 (MQ=255)                                                                                                                                             gCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCCGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGTCTTCTTCAATAGCGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGTACCGAGCATTATGTATGtttt                                                                                                                         >  2:98125/1‑300 (MQ=255)                                                                                                                                             gCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCAGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTAAGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGtttt                                                                                                                         >  2:339112/1‑300 (MQ=255)                                                                                                                                                  aaGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATANCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGAACAGCACCGAGCCTTCTGCCTGTTTTGtata                                                                                                                    >  2:227474/1‑300 (MQ=255)                                                                                                                                                       cGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGAACAGCACCGAGCCTTCTGCATGTTTTGTATAGTTAc                                                                                                               >  1:207474/1‑300 (MQ=255)                                                                                                                                                                  aGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGt                                                                                                     <  1:328552/299‑1 (MQ=255)                                                                                                                                                                      taatcttGAACTAACCCAGCGCTTGCAAATCCACTGCCGCGCTAAGGACACGGGCGAGCAGACGCTGGATTGCCATTGTGCTTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCTTTACGTGTGTGGTTAGAACGCTGCAGCATATCGTACTCTGCGGTGATCCAGCCCTGGCCCTTACCTTTCAGGAAGCGATGCACGCCTTCTCCAATATAGGCGGTGCACAATACCTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTTTATAGTTACGAGTCAGGGTAACgg                                                                                                <  2:57401/294‑1 (MQ=255)                                                                                                                                                                      tAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACgg                                                                                                <  2:235006/300‑1 (MQ=255)                                                                                                                                                                      tAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACgg                                                                                                <  1:618341/300‑1 (MQ=255)                                                                                                                                                                      tAATGGTGAACCCACCCATCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCTAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGTTAACgg                                                                                                <  2:82738/300‑1 (MQ=255)                                                                                                                                                                      gaaTGGGGAAATCACCAAGCGCGTGCAAATCGAATGCCGCGCGAAGTGAACGGGAGATCAGACGATGGAGTGCCATTGTGCGTCCACCCTGCTTACCTGGCGCTGCTTCACGAGCGTGACGGGTGTGGGTAGAAAGCGGCAGCATACAGGACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGACACGCCTTCTGCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCAGGTTTGGTAGAGTGACGAGTCAGGGGAACgg                                                                                                <  2:439526/299‑1 (MQ=255)                                                                                                                                                                        aTGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAAc                                                                                                  >  2:244093/1‑296 (MQ=255)                                                                                                                                                                        aTGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAAc                                                                                                  >  2:308715/1‑296 (MQ=255)                                                                                                                                                                        aTGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGa                                                                                              >  1:79806/1‑300 (MQ=255)                                                                                                                                                                        aTGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCAACGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGa                                                                                              >  2:395753/1‑300 (MQ=255)                                                                                                                                                                        aTGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTAAGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCATTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGa                                                                                              >  1:372271/1‑300 (MQ=255)                                                                                                                                                                                  cACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGNGTTACGGGTGTGGGTAGAACGCGGCAGCATACNGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGat                                                                                    >  2:526809/1‑300 (MQ=255)                                                                                                                                                                                  cACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGNGTTACGGGTGTGGGTAGAACGCGGCAGCATACNGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTAAGGAAGCGAGGCACGCCTTCTTCCATAGAGGCGGTCCACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCATTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGat                                                                                    >  2:526825/1‑300 (MQ=255)                                                                                                                                                                                   aCCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGatt                                                                                   >  1:668556/1‑300 (MQ=255)                                                                                                                                                                                   aCCCAGCGCTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAAAGGGACGCACCTGatt                                                                                   >  2:41610/1‑300 (MQ=255)                                                                                                                                                                                        gcgcTTTCAGATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGc                                                                              <  1:613797/300‑1 (MQ=255)                                                                                                                                                                                        gcgcTTTCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGc                                                                              <  1:434812/300‑1 (MQ=255)                                                                                                                                                                                            tttCAAATCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTAc                                                                           >  1:531110/1‑299 (MQ=255)                                                                                                                                                                                              tCAAATCTACTGCCGCGCGAAGTGCACGGTCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGTTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATATTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGatt                                                                                   <  2:195306/289‑1 (MQ=255)                                                                                                                                                                                                  aTCTACTGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCctgc                                                                    >  1:622777/1‑300 (MQ=255)                                                                                                                                                                                                        tGCCGCGCGAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTATGGGTGTGGGTAGAACGCTGCAGCATACCGTACTCTGCGGTGATCCAGCCCTTGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACTGGACGCACCTGATTATTGCTACGGCCTGCTGGAc                                                               <  2:126732/299‑1 (MQ=255)                                                                                                                                                                                                        tGCCGCGCGAAATGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGAc                                                               <  1:659603/299‑1 (MQ=255)                                                                                                                                                                                                              gcgAAGTGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGTAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATAtt                                                        >  1:692476/1‑300 (MQ=255)                                                                                                                                                                                                                   gtgCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACNTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAAt                                                   >  2:498915/3‑300 (MQ=255)                                                                                                                                                                                                                   gtgCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCATCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAAt                                                   >  1:699537/3‑300 (MQ=255)                                                                                                                                                                                                                                 aGACGCTGGATTTCCATTGTGTGTCCACCTTGCTTACCTTTCGCTGCTTCACGAGCGTTAAGGGTGTGGGTAGAACGCTGCAGCATACCGTACTCTGCGGGGATCCAGCCCTTGCCCTGACCTTTCAGGAAGCTAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAAc                                     <  2:41347/300‑1 (MQ=255)                                                                                                                                                                                                                                        ggATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAAtgt                               >  1:700473/1‑299 (MQ=255)                                                                                                                                                                                                                                             tCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCt                           >  2:38775/1‑298 (MQ=255)                                                                                                                                                                                                                                             tCCATTGTGCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCACTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGTATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCAGGCTTGAAACAAATGTGGCt                           >  2:113760/1‑298 (MQ=255)                                                                                                                                                                                                                                                   gtgCGTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCCGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATta                   >  2:620941/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gtccgccatacTTACCTTTCGCTGCTTCACGAGCGTTACGAGTGTGGGGAGAACGCGGCAGCATACCATACTCTGCGGTGATCCAACCCTGGCCCTGACCTTTCAGTAAACGGGGCGCGCCTTCTTCAATAGNGGCGGTACGCAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATTTTTTGTATAGTTACGAGTCAGGTTAACTGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATGGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATAc                <  2:215012/289‑1 (MQ=255)                                                                                                                                                                                                                                                       gTCCACCCTGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATAc                <  1:637835/299‑1 (MQ=255)                                                                                                                                                                                                                                                               tGCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTcc        >  2:301932/1‑299 (MQ=255)                                                                                                                                                                                                                                                                gCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACACTACTTTGGTATCGCCAAATTAGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATCCGGACTTcc        >  2:112601/1‑298 (MQ=255)                                                                                                                                                                                                                                                                gCTTACCTTTCGCTGCTTCACGAGCGTTACGGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTcc        >  1:374293/1‑298 (MQ=255)                                                                                                                                                                                                                                                                    acccttCGCTGCTTCACGAGCGTTACAGGTGTGGGTAGAACGCGGCAGCATACCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGAGGCACGCCTTCTTCAATAGAGGCGGTACACAATACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGt  <  2:117632/296‑1 (MQ=255)                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                   TCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACGGGTGTGGGTAGAACGTGGCAGCATGCCGTACTCTGCGGTGATCCAGCCCTGGCCCTGACCTTTCAGGAAGCGCGGCACGCCTTCTTCAATAGAGGCGGTACACAACACTTTGGTATCGCCAAATTCGACCAGCACCGAGCCTTCTGCATGTTTTGTATAGTTACGAGTCAGGGTAACGGGACGCACCTGATTATTGCTACGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGT  >  NC_000913/3816048‑3816606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑