Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,813,342	G→C	P204P (CCG→CCC)	dfp →	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,813,342	0	G	C	96.5%	183.5 / NA	58	P204P (CCG→CCC)	dfp	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (32/24);  minor base A (2/0);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGCCCCAGAGAA‑AATCAAAAAGCAGGCCACGCAGGGTGATGAAT  >  NC_000913/3813057‑3813628                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                 gaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTATAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATAtctc                                                                                                                                                                                                                                                                                    <  1:158508/300‑1 (MQ=255)          ggCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGTAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATCTGGCAGTAGCTCATTTTTCGCCCGTCCACGACCTGAAACATCTGAACATTATGATTCCCACCGGCCCGACGCGTGAACCGTTCGATCCCGTGCGTTGTATCTCTAAttgca                                                                                                                                                                                                                                                                            >  2:31952/1‑295 (MQ=255)               cacCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTcc                                                                                                                                                                                                                                                                       >  1:372678/1‑299 (MQ=255)                      gcCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTACTCACAGCTCCGGCAAGAt                                                                                                                                                                                                                                                               >  2:614565/1‑300 (MQ=255)                      gcCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAt                                                                                                                                                                                                                                                               <  1:677163/300‑1 (MQ=255)                             gCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCAGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTCTATCTCTAATCACAGCTCCGGCAAGAT‑GGGtttt                                                                                                                                                                                                                                                       >  2:418349/1‑300 (MQ=255)                              ccGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGtttt                                                                                                                                                                                                                                                       >  2:659284/1‑299 (MQ=255)                              ccGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGAATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCAGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGtttt                                                                                                                                                                                                                                                       >  2:659291/1‑299 (MQ=255)                                                     cagATGTACCGTGTCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGGTCGATCCCGTGCGTTATATCTCTAATCAGAGCTCCGGCAAGAt                                                                                                                                                                                                                                                               >  2:583231/1‑269 (MQ=255)                                                     cagATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCACGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGGTCGATCCCGTGCGTTATATCTCTAATCAGAGCTCCGGCAAGATCGGGTTTTGCTATCGCCGCCGCCGCt                                                                                                                                                                                                                                     >  2:583221/1‑295 (MQ=255)                                                                                  tactttAGGGGTGCTTGCTTCCCTTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCATGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAgg                                                                                                                                                                                                  <  2:177198/297‑1 (MQ=255)                                                                                  tAATTTATAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAgg                                                                                                                                                                                                  <  1:557284/300‑1 (MQ=255)                                                                                  tAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAgg                                                                                                                                                                                                  <  1:695248/300‑1 (MQ=255)                                                                                    aTTTAGCGGTGCTTACTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCCGGCTTGTGGAGATATCGGCCCTGGGCGAATACTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGCCCTGAAACATCTGACCATTATGCTTACCGCCGGCCCGAAGCGTGAACCGCTCGATCCAGTGCGTTATATCTATAATCACAGCTCCGGCAAGAG‑AGGTTTTGCTATCGCCGCAGCCGCTGCACGTCGTGGCGTGAAAGTCAAGCTGGTATAAGGTc                                                                                                                                                                                                >  2:28371/1‑300 (MQ=255)                                                                                    aTTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTc                                                                                                                                                                                                >  1:459746/1‑300 (MQ=255)                                                                                                         gTGGTTGGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGcc                                                                                                                                                                             <  2:601048/298‑1 (MQ=255)                                                                                                              ttGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGttt                                                                                                                                                                      >  1:37309/1‑300 (MQ=255)                                                                                                                gCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTATAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGt                                                                                                                                                                    >  2:9254/1‑300 (MQ=255)                                                                                                                          gggCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGtt                                                                                                                                                          >  1:329493/1‑300 (MQ=255)                                                                                                                          gggCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGtt                                                                                                                                                          >  2:139767/1‑300 (MQ=255)                                                                                                                                    gTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGtgatga                                                                                                                                                >  2:481383/1‑300 (MQ=255)                                                                                                                                    gTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCACGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTATAATCAAAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAAGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGtgatga                                                                                                                                                >  2:251054/1‑300 (MQ=255)                                                                                                                                     tGGCAGTCAGGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTtgatg                                                                                                                                                      <  1:188633/293‑1 (MQ=255)                                                                                                                                     tGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCTGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTtgatgtg                                                                                                                                                    <  2:334089/295‑1 (MQ=255)                                                                                                                                     tGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTtgatg                                                                                                                                                      <  1:189704/293‑1 (MQ=255)                                                                                                                                     tGGCAGGCAGGCTTGTTGAGATATCGGCTCTGGTCAAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATANCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGNTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGAc                                                                                                                                               <  2:525917/300‑1 (MQ=255)                                                                                                                                         attCAGGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTTTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCGGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCTGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCTTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTtgatgtgatg                                                                                                                                                 <  2:38197/292‑1 (MQ=255)                                                                                                                                              ggCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCAATACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGa                                                                                                                                      >  2:200395/1‑300 (MQ=255)                                                                                                                                                cTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGaaa                                                                                                                                    >  2:469576/1‑300 (MQ=255)                                                                                                                                                cTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGaaa                                                                                                                                    >  1:120158/1‑300 (MQ=255)                                                                                                                                                    tggagATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGa                                                                                                                                <  1:409364/296‑1 (MQ=255)                                                                                                                                                        gATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAgc                                                                                                                             >  1:251628/1‑299 (MQ=255)                                                                                                                                                               gcccTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGa                                                                                                                     >  1:291504/3‑300 (MQ=255)                                                                                                                                                                      gCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTc                                                                                                              <  1:235693/300‑1 (MQ=255)                                                                                                                                                                           tacTCGATCCGTGAACCATTGTGGATATGGCAATAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCTGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATTTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTAc                                                                                                         <  2:470249/298‑1 (MQ=255)                                                                                                                                                                           tGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTAc                                                                                                         <  1:100339/300‑1 (MQ=255)                                                                                                                                                                                     gTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCcaaa                                                                                               >  1:237475/1‑300 (MQ=255)                                                                                                                                                                                     gTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCaaaa                                                                                               >  1:86847/1‑300 (MQ=255)                                                                                                                                                                                       tAACCATTGTGGATATGGCAGTAGCTCATTGTTCGCACGGCAACGATCTGAAACATCTGAACATTATGATTACCGCCGGCCTGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCATAGCTCCGGTAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACTTCGCGCTGGTATCAGGTCTGGTTTCACTACCGACGCCACCGTTTGTTAAGCGCGTTGATTTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTAcagc                                                                                                      <  2:76228/291‑1 (MQ=255)                                                                                                                                                                                          ccATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATAttt                                                                                          >  1:377283/1‑300 (MQ=255)                                                                                                                                                                                            aTTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATAttttt                                                                                        >  2:609972/1‑300 (MQ=255)                                                                                                                                                                                            aTTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATAttttt                                                                                        >  1:390589/1‑300 (MQ=255)                                                                                                                                                                                                                         gCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCAGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATcg                                                           <  1:635910/300‑1 (MQ=255)                                                                                                                                                                                                                            cGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATcgct                                                         >  2:561987/1‑298 (MQ=255)                                                                                                                                                                                                                                    aCCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTAcc                                                 >  1:530943/1‑299 (MQ=255)                                                                                                                                                                                                                                    aCCTGAAACATCTGAACATTATGAGTACCGCCGGCCCGACACGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATTGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGTTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCg                                                <  2:358841/300‑1 (MQ=255)                                                                                                                                                                                                                                       tGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTg                                              <  1:59422/299‑1 (MQ=255)                                                                                                                                                                                                                                        gAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGt                                               >  2:568751/1‑297 (MQ=255)                                                                                                                                                                                                                                         aaaCATCTGAACATTATGATTACCGCCGGCCCTACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGcc                                           <  2:459402/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gaataTTATGACTACCGCCGGCCCGATGCGTGAACCGTTCGATCCCGTGCTTTATATCTCTAATCACAGCTTCGGCAAGAT‑GGTTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTAc                                                  <  2:26348/281‑1 (MQ=255)                                                                                                                                                                                                                                                    cATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGCCCCAGAAAA‑AAt                               >  1:622292/1‑300 (MQ=255)                                                                                                                                                                                                                                                     aTTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGCCCAAGAAAA‑AATc                              >  1:194197/1‑300 (MQ=255)                                                                                                                                                                                                                                                            cctgcaagatgtgtatcagagacaggCTCGATCCCGTGCGTTGTATCTCTCCTCACAGCCCCAGAAAGAT‑GAGTTTTGCTATCAACGCCGCCACTGCCCGTCGTGGCGCGACCGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTCAACGTGTTGATATGATGACCGCGCTGGAAATGGAAGCCACCGTGAATGCTTCTGTCCAGCAGCCAAATATTTTTATCGACTGCACAGCCGTGGCGGATTATCGCGCAACTACCGTGGCACCAG‑GAAGAATCAAACAGc                       >  1:179373/26‑300 (MQ=255)                                                                                                                                                                                                                                                                          aCGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGCCCCAGAAAA‑AATCAAAAAGCAGGCCACGCAGGGt         <  1:400730/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           cgcgTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGGT‑GGGTTTTGCTATCGCCGCCGCCGCTGCACGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTAACGACGCCACCGTTTGTTAAGCGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGCACCAGAAAA‑AATCAAAAAGCAGGCAAAGCAGGGt         >  2:56773/1‑299 (MQ=255)                                                                                                                                                                                                                                                                           cgcgTGAACCGCTCGATCCCGTGCGTTATATCTATAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCAACGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTAACGTGGCCCCAGAAAA‑AATCAAAAAGAAGGCCAAGCAGGGt         >  2:47647/1‑299 (MQ=255)                                                                                                                                                                                                                                                                               taaaCCGCTCGATCCCATGCGTTATATCTCTAATCACAACTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGCCCCAGAAAA‑AATCAAAAAGCAGGCCACGCAGGGtgatga    <  2:372678/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aaCCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGCCCCAGAAAA‑AATCAAAAAGCAGGCCACGCAGGGTGATGAAt  <  2:10791/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                 GATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTTATCGGCTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGCCCCAGAGAA‑AATCAAAAAGCAGGCCACGCAGGGTGATGAAT  >  NC_000913/3813057‑3813628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑