Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,813,264	G→T	A178A (GCG→GCT)	dfp →	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,813,264	0	G	T	100.0%	208.1 / NA	59	A178A (GCG→GCT)	dfp	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/26);  total (33/26)

GATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTT  >  NC_000913/3812977‑3813543                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                         gATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGccc                                                                                                                                                                                                                                                                              >  2:409364/1‑300 (MQ=255) gATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGccc                                                                                                                                                                                                                                                                              >  1:358841/1‑300 (MQ=255)  aTTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGccc                                                                                                                                                                                                                                                                              >  1:707021/1‑299 (MQ=255)           ttCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGAcc                                                                                                                                                                                                                                                                    <  2:683269/300‑1 (MQ=255)               cGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGaa                                                                                                                                                                                                                                                                >  1:10791/1‑300 (MQ=255)                         aCGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAAc                                                                                                                                                                                                                                                      <  1:638391/300‑1 (MQ=255)                          cGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACa                                                                                                                                                                                                                                                     >  2:635910/1‑300 (MQ=255)                              aGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTAt                                                                                                                                                                                                                                                 >  2:690230/1‑300 (MQ=255)                                aTTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGa                                                                                                                                                                                                                                               <  1:369438/300‑1 (MQ=255)                                  tggagggCCCGTGTTGCTGCTGGAATTGCGAATGACATGGTATCGACAGTTTGTTTGTCTACGCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCGGGGTGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCGTTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGAtt                                                                                                                                                                                                                                             <  2:365041/294‑1 (MQ=255)                                                        gAATGGCGAATGACCTGTTATCGACGATTTGTCTTGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCTCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCGCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTTAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAAc                                                                                                                                                                                                                       <  2:80990/300‑1 (MQ=255)                                                        gAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAAc                                                                                                                                                                                                                       <  1:164623/300‑1 (MQ=255)                                                        gAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGACACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAAc                                                                                                                                                                                                                       <  1:556412/300‑1 (MQ=255)                                                                aaTGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGAt                                                                                                                                                                                                               <  1:236339/300‑1 (MQ=255)                                                                  tGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATcc                                                                                                                                                                                                             <  1:274716/300‑1 (MQ=255)                                                                  tGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATcc                                                                                                                                                                                                             <  1:121091/300‑1 (MQ=255)                                                                   gACCTGGTATCGAAGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATcc                                                                                                                                                                                                             >  1:212410/1‑299 (MQ=255)                                                                                 gaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTATAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATAtctc                                                                                                                                                                                              <  1:158508/300‑1 (MQ=255)                                                                                          ggCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGTAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATCTGGCAGTAGCTCATTTTTCGCCCGTCCACGACCTGAAACATCTGAACATTATGATTCCCACCGGCCCGACGCGTGAACCGTTCGATCCCGTGCGTTGTATCTCTAAttgca                                                                                                                                                                                      >  2:31952/1‑295 (MQ=255)                                                                                               cacCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTcc                                                                                                                                                                                 >  1:372678/1‑299 (MQ=255)                                                                                                      gcCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTACTCACAGCTCCGGCAAGAt                                                                                                                                                                         >  2:614565/1‑300 (MQ=255)                                                                                                      gcCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAt                                                                                                                                                                         <  1:677163/300‑1 (MQ=255)                                                                                                             gCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCAGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTCTATCTCTAATCACAGCTCCGGCAAGAT‑GGGtttt                                                                                                                                                                 >  2:418349/1‑300 (MQ=255)                                                                                                              ccGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGtttt                                                                                                                                                                 >  2:659284/1‑299 (MQ=255)                                                                                                              ccGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGAATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCAGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGtttt                                                                                                                                                                 >  2:659291/1‑299 (MQ=255)                                                                                                                                     cagATGTACCGTGTCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGGTCGATCCCGTGCGTTATATCTCTAATCAGAGCTCCGGCAAGAt                                                                                                                                                                         >  2:583231/1‑269 (MQ=255)                                                                                                                                     cagATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCACGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGGTCGATCCCGTGCGTTATATCTCTAATCAGAGCTCCGGCAAGATCGGGTTTTGCTATCGCCGCCGCCGCt                                                                                                                                               >  2:583221/1‑295 (MQ=255)                                                                                                                                                                  tactttAGGGGTGCTTGCTTCCCTTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCATGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAgg                                                                                                            <  2:177198/297‑1 (MQ=255)                                                                                                                                                                  tAATTTATAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAgg                                                                                                            <  1:557284/300‑1 (MQ=255)                                                                                                                                                                  tAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAgg                                                                                                            <  1:695248/300‑1 (MQ=255)                                                                                                                                                                    aTTTAGCGGTGCTTACTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCCGGCTTGTGGAGATATCGGCCCTGGGCGAATACTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGCCCTGAAACATCTGACCATTATGCTTACCGCCGGCCCGAAGCGTGAACCGCTCGATCCAGTGCGTTATATCTATAATCACAGCTCCGGCAAGAG‑AGGTTTTGCTATCGCCGCAGCCGCTGCACGTCGTGGCGTGAAAGTCAAGCTGGTATAAGGTc                                                                                                          >  2:28371/1‑300 (MQ=255)                                                                                                                                                                    aTTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTc                                                                                                          >  1:459746/1‑300 (MQ=255)                                                                                                                                                                                         gTGGTTGGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGcc                                                                                       <  2:601048/298‑1 (MQ=255)                                                                                                                                                                                              ttGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGttt                                                                                >  1:37309/1‑300 (MQ=255)                                                                                                                                                                                                gCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTATAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGt                                                                              >  2:9254/1‑300 (MQ=255)                                                                                                                                                                                                          gggCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGtt                                                                    >  2:139767/1‑300 (MQ=255)                                                                                                                                                                                                          gggCCAGACAGTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGtt                                                                    >  1:329493/1‑300 (MQ=255)                                                                                                                                                                                                                    gTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGtgatga                                                          >  2:481383/1‑300 (MQ=255)                                                                                                                                                                                                                    gTGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCACGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTATAATCAAAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCAAGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGtgatga                                                          >  2:251054/1‑300 (MQ=255)                                                                                                                                                                                                                     tGGCAGTCAGGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTtgatg                                                                <  1:188633/293‑1 (MQ=255)                                                                                                                                                                                                                     tGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCTGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTtgatgtg                                                              <  2:334089/295‑1 (MQ=255)                                                                                                                                                                                                                     tGGCAGTCAGGCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTtgatg                                                                <  1:189704/293‑1 (MQ=255)                                                                                                                                                                                                                     tGGCAGGCAGGCTTGTTGAGATATCGGCTCTGGTCAAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATANCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGNTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGAc                                                         <  2:525917/300‑1 (MQ=255)                                                                                                                                                                                                                         attCAGGCTTGTGTAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTTTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCGGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCTGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCTTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTtgatgtgatg                                                           <  2:38197/292‑1 (MQ=255)                                                                                                                                                                                                                              ggCTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCAATACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGa                                                >  2:200395/1‑300 (MQ=255)                                                                                                                                                                                                                                cTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGaaa                                              >  2:469576/1‑300 (MQ=255)                                                                                                                                                                                                                                cTTGTGGAGATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGaaa                                              >  1:120158/1‑300 (MQ=255)                                                                                                                                                                                                                                    tggagATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGa                                          <  1:409364/296‑1 (MQ=255)                                                                                                                                                                                                                                        gATATCGGCCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAgc                                       >  1:251628/1‑299 (MQ=255)                                                                                                                                                                                                                                               gcccTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGa                               >  1:291504/3‑300 (MQ=255)                                                                                                                                                                                                                                                      gCGAATGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTc                        <  1:235693/300‑1 (MQ=255)                                                                                                                                                                                                                                                           tacTCGATCCGTGAACCATTGTGGATATGGCAATAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCTGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATTTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTAc                   <  2:470249/298‑1 (MQ=255)                                                                                                                                                                                                                                                           tGCTCGATCCGTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTAc                   <  1:100339/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     gTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCcaaa         >  1:237475/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gTTAACCATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCaaaa         >  1:86847/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       tAACCATTGTGGATATGGCAGTAGCTCATTGTTCGCACGGCAACGATCTGAAACATCTGAACATTATGATTACCGCCGGCCTGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCATAGCTCCGGTAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACTTCGCGCTGGTATCAGGTCTGGTTTCACTACCGACGCCACCGTTTGTTAAGCGCGTTGATTTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTAcagc                <  2:76228/291‑1 (MQ=255)                                                                                                                                                                                                                                                                          ccATTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATAttt    >  1:377283/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            aTTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATAttttt  >  1:390589/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            aTTGTGGATATGGCAGTAGCTCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCCGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATAttttt  >  2:609972/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                         GATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAT‑GGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCAGCAAAATATTTTT  >  NC_000913/3812977‑3813543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑