Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,093	T→C	I55I (ATT→ATC)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,093	0	T	C	100.0%	215.8 / NA	62	I55I (ATT→ATC)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/36);  total (26/36)

GCTATCAGGCGTTCACTCAGGCAGATCTGACAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCCAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCT  >  NC_000913/3794798‑3795380                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                gCTATCATGCGTTCACTCAGGCAGATCTGACGAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTGAACGAAGCTATCCCTa                                                                                                                                                                                                                                                                                             <  2:457185/300‑1 (MQ=255)       aGGCGTTCACTCAGGCAGATCTGACGAATCTGCGCGCTGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGcc                                                                                                                                                                                                                                                                                         <  2:5779/298‑1 (MQ=255)              cACTCAGGCAGATCTGACGAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCa                                                                                                                                                                                                                                                                                >  2:630272/1‑300 (MQ=255)                 tCAGGCAGATCTGACGAATCTGCGCGCGGCGGGTTACGACAAACCGTGCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACgg                                                                                                                                                                                                                                                                             <  1:265219/300‑1 (MQ=255)                   aGGCAGATCTGACGAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAg                                                                                                                                                                                                                                                                           <  1:645708/300‑1 (MQ=255)                    ggCAGATCTGACGAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACgg                                                                                                                                                                                                                                                                             <  1:263451/297‑1 (MQ=255)                        gATCTGACGAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCNGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCt                                                                                                                                                                                                                                                                       >  1:143062/1‑299 (MQ=255)                        gATCTGACGAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCNGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTATCGGTCACGGAGCGCt                                                                                                                                                                                                                                                                       >  1:141646/1‑299 (MQ=255)                            tGACGAATCTGCGCGCGTCGGGTTACGACAAACCATTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGTTGATCGGCCCTTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGaa                                                                                                                                                                                                                                                                   <  2:28465/299‑1 (MQ=255)                            tGACGAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGaa                                                                                                                                                                                                                                                                   <  1:572512/299‑1 (MQ=255)                              acgaaTCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAAGACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATc                                                                                                                                                                                                                                                                <  2:487965/297‑1 (MQ=255)                                                gggtaagACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCATGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGNAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCTGTCAAGGAGCGCTGGAAATCGGCGAACGCCGCaagct                                                                                                                                                                                                                                               >  2:505953/7‑296 (MQ=255)                                                ggTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGNAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCACAGGCGATAATCGCTGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTCTGCCTCTAGGTCACGTAGCGCTGGAAACCGGCGAACGCCGCAAACt                                                                                                                                                                                                                                               >  2:505954/1‑299 (MQ=255)                                                ggTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACt                                                                                                                                                                                                                                               >  1:449402/1‑299 (MQ=255)                                                ggTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACt                                                                                                                                                                                                                                               >  2:307245/1‑299 (MQ=255)                                                ggTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCAGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTATCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCaagct                                                                                                                                                                                                                                               >  2:307530/1‑296 (MQ=255)                                                    aCGACAAACCGTTCAAAACCGTTGCTGAAGGTTTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTc                                                                                                                                                                                                                                          <  2:447358/300‑1 (MQ=255)                                                         aaaCCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATTTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAg                                                                                                                                                                                                                                      <  1:163366/299‑1 (MQ=255)                                                          aaCCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGcc                                                                                                                                                                                                                                    <  1:356469/300‑1 (MQ=255)                                                                                          gAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCNACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTc                                                                                                                                                                                                    <  1:142961/300‑1 (MQ=255)                                                                                              aCATTGCCTTGCTTAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCTGCCCTTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCATGCGCGCTATCCCCAGGCGATAATCGATTTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGGAAAGCGCTACGACCGCGCCTACGTCTTAc                                                                                                                                                                                                <  2:60655/300‑1 (MQ=255)                                                                                                      tGGCTGAAGCGCGGCGCATAAGAGCTCTGTATAAAAATACTGTTGATCGGCCCGTCTTGGGGTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATTTGATGGCACCTGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACGGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACt                                                                                                                                                                                          <  2:88291/298‑1 (MQ=255)                                                                                                        gCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCtt                                                                                                                                                                                      >  1:424642/1‑300 (MQ=255)                                                                                                                   aCGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGANGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTCCGTCTTACCAAACTCCTTCAAATCTGCAt                                                                                                                                                                           >  2:205834/1‑300 (MQ=255)                                                                                                                      cATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCTGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTa                                                                                                                                                                         >  1:407553/1‑299 (MQ=255)                                                                                                                      cATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTa                                                                                                                                                                         >  1:617510/1‑299 (MQ=255)                                                                                                                      cATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCAGGAAGTTAACGAAGCTATCCATATGCCTATCGGTCACGGAGCGATGGAAATCGGCGAACGTGGCAAACTGGGTCATAGCCTGCGTGAAGAGCGATACGACCGCGACTACGTCTTAACAAATTCCTCTAAATCTGCAt                                                                                                                                                                           >  2:142280/1‑297 (MQ=255)                                                                                                                       ctgcgagatgtgtataagagacagggTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGt                                                                                                                                                                       >  1:88867/25‑300 (MQ=255)                                                                                                                                ctGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTATCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGAACGCGCATACGTCTTAACAAATTCATTAAAATCTGCATTAGTGCCttt                                                                                                                                                                 >  2:86402/1‑297 (MQ=255)                                                                                                                                                  gATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCTGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGGTAACTAAGCTATCCCTATGCCTCTCGGTCACTGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAACGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTTTTCGCGGGTATTCCTCATc                                                                                                                                             <  2:71699/299‑1 (MQ=255)                                                                                                                                                   atctgcccgcctttcgtctgcaaCATGATGATTTCGCAAAGTCTCTATCGCACGCTCTAGGCGCGCTATCCCCAGGAGATAATCGATGTGATGGCACCGGCACGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGAGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCGACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGc                                                                                                                                           <  2:357288/278‑1 (MQ=255)                                                                                                                                                   aTCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTACCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGc                                                                                                                                           <  2:353096/300‑1 (MQ=255)                                                                                                                                                                gggTTGGCGACATGATNATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTNTCTTCGCGGGTATTCCTCNTCGCACCGGCTGgcgc                                                                                                                               >  1:588924/1‑299 (MQ=255)                                                                                                                                                                                      gCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTAc                                                                                                         <  1:353378/299‑1 (MQ=255)                                                                                                                                                                                            tctcTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTTCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGa                                                                                                  <  2:444656/300‑1 (MQ=255)                                                                                                                                                                                            tctcTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCTAGATGCGCTACGGTTTACTCAACGa                                                                                                  <  1:413422/300‑1 (MQ=255)                                                                                                                                                                                            tctcTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGa                                                                                                  <  1:88173/300‑1 (MQ=255)                                                                                                                                                                                                    gCACGCTCCATGCGCGCTATCCCCATGCGATAATCGATGTGATTGCACCTGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAGCGAAGCTATCCCTATGCCTCTCGGTCACTGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGTTCATAGCCTGCGTTAAAAGCGCTACGACCGCGCCTACTTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGTTATTCCTCATTGCACCGGTTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTAcgcg                                                                                          <  2:195061/300‑1 (MQ=255)                                                                                                                                                                                                         cTCCAGGCGCGCTATCCCCAGGCGATAATTGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCAGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCAGCTGGCGCGGCGAGATGCGCTAAGGTTTACTCAACGATGTACGCGTGCTc                                                                                     >  1:470084/1‑300 (MQ=255)                                                                                                                                                                                                         cTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTc                                                                                     >  2:422503/1‑300 (MQ=255)                                                                                                                                                                                                         cTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTAATCAACGATGTACGCGTGCTc                                                                                     >  2:2812/1‑300 (MQ=255)                                                                                                                                                                                                         cTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGACATGGTGCAGTCCATTATTATCGCGGATGCAGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAATGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGACTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTAGAGGGTATTCTTCATCGCCCCGGCTGGCGCGGCGAGATGCGCTACGGTCTACTCAACGATGTACGCGTGCt                                                                                      >  2:627166/1‑299 (MQ=255)                                                                                                                                                                                                                     tATCCCCATGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGcc                                                                         <  1:182852/300‑1 (MQ=255)                                                                                                                                                                                                                      aTCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCt                                                                        >  1:116407/1‑300 (MQ=255)                                                                                                                                                                                                                                gATAGTCGATTTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGTGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAaagaag                                                                           <  2:11864/287‑1 (MQ=255)                                                                                                                                                                                                                                               ggCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGGGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTACTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCtatgt                                               <  2:617670/300‑3 (MQ=255)                                                                                                                                                                                                                                                 cACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTATCGGTCACGGAGCGATGGAAATCGGCGAACGCAGCAAACTGGGTAATAGCCTGCGTGAAAAGTGCTACGACCGCGCCTACGTCTTAACAAACTCCTTAAAATCTGCATTAGTGCCTTTCTTAGCGGGTATTCCTCATCGCACCGGTTGGCGCGGCGAGATGCGCTAAGGTTTACTCAACGATGTACGCGTGTTCGACAAGGAAGCCTGGCCGCTAATGGTGGAACGCtatgt                                               >  2:353479/1‑296 (MQ=255)                                                                                                                                                                                                                                                      gCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCtatgtcgcgc                                          <  1:205834/298‑8 (MQ=255)                                                                                                                                                                                                                                                      gCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCt                                         >  2:453017/1‑299 (MQ=255)                                                                                                                                                                                                                                                      gCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGAAGACTGGGCCATAGCCTGCGTGACAAGCGCTACGACCGCGACTACGTCTTAACAAACTAATTAAAATCTGCATTAGTGCCTTTCTTCGAGGGTATTCCTCATCGCACCGGCTGNCGAGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCtatgtcgagct                                         >  2:540282/1‑291 (MQ=255)                                                                                                                                                                                                                                                         tGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGcc                                     <  1:538019/300‑1 (MQ=255)                                                                                                                                                                                                                                                               cGTCCATTATTATCGCGGATGTCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGa                                >  1:28563/1‑299 (MQ=255)                                                                                                                                                                                                                                                                gTCCATTATTCTCGCGGATGTCTGAAGTTAACGAAGCTCGCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCTCAAACTGGTTCATAGCCTGCGGGAAAAGCGCGACGACCGCGCCTACGTCTTACCAAACCCCTTCAAATCGGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCGACGGTTTACTCAAc                                                                                                    <  2:96562/230‑1 (MQ=255)                                                                                                                                                                                                                                                                gTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGAc                               <  1:159369/299‑1 (MQ=255)                                                                                                                                                                                                                                                                gTCCATTATGATCACGGATGCCGGAAGGTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCGACGACCACGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGAc                               <  2:244872/299‑1 (MQ=255)                                                                                                                                                                                                                                                                           tcgtggATGCCGGAAGTGAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATg                   <  2:167843/296‑1 (MQ=255)                                                                                                                                                                                                                                                                           tCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATg                   <  1:453017/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATgcgtac              <  1:646974/300‑4 (MQ=255)                                                                                                                                                                                                                                                                                gATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATg                   <  2:438648/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aTGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCTGTCACGGAGCGCTAGAAATCGTCGAACGCCGCAAACTGGGTCATAGCCTACGTTAAAAGCGCTACGAACGCGCCTACTTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATCCCTCATCGCACCGTCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATgcgtacc             <  2:328879/300‑5 (MQ=255)                                                                                                                                                                                                                                                                                  ggCCGGAAGTTAACGAAACTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTTGGTCATAGCCTGCGTTAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCTAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATTTCGCGCTGGCCTATGACAAGGGCATTATgcgtacc             <  2:326911/298‑5 (MQ=255)                                                                                                                                                                                                                                                                                             aaCGAAGCTATCCCTATGCCTCTCGGTCACGGAGTGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAAAGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAACGGCATTATGCGTACCGCACAAGATCt  >  2:118650/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                GCTATCAGGCGTTCACTCAGGCAGATCTGACAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCCAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCT  >  NC_000913/3794798‑3795380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑