Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,997	T→C	S185S (TCA→TCG)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,997	0	T	C	100.0%	232.6 / NA	65	S185S (TCA→TCG)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/35);  total (30/35)

GGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTTATCTGCCAGATCGCAAACGCCCTTCAGGTTGGCAATCACGCCGTCCATTGAGAACACACCATCGGTGGCGATCAGCACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTGCACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGC  >  NC_000913/3791703‑3792289                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     ggcgacaaTAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGCCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGaa                                                                                                                                                                                                                                                                                                 >  1:402127/5‑300 (MQ=255)     acaaTAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAacaca                                                                                                                                                                                                                                                                                             >  1:632511/1‑300 (MQ=255)              ggTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGTTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGANCCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACACCCTTCAGATTGGCAAGTACGCCGGCCATCGAGAACACACCATCGGTg                                                                                                                                                                                                                                                                                    <  2:497484/300‑1 (MQ=255)               gTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACAAGGGCATCGTATTTATCTGCCCGATCGCAAACGCCCTTCAGATTGGCCATCACGCCGTCCATCGAGAACACACCATCGGt                                                                                                                                                                                                                                                                                     >  2:328198/1‑298 (MQ=255)                               gaACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGAcg                                                                                                                                                                                                                                                                   <  1:121647/300‑1 (MQ=255)                                    ggTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCTGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCAc                                                                                                                                                                                                                                                              >  1:265783/1‑300 (MQ=255)                                          gccGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCCTCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCtt                                                                                                                                                                                                                                                        >  1:266323/3‑300 (MQ=255)                                          gACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCtt                                                                                                                                                                                                                                                        >  1:266835/1‑300 (MQ=255)                                          gACGAGAACGCGGGCGCAGCCACTCAACCATTTCTTTGCGCGCCGCTGGATAACCACCAGAATCCCCGCCCAGCGCTCTACCAAGTTTGCCGTTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTCCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTCATCTTCCAGATCACAAACGCCCTCCAGGTTTGCGAGCACGCCGGCCACCGAGAACACACCATCGGTGGCAATcctgtctcttatacacatctcgc                                                                                                                                                                                                                                                        <  2:160035/300‑23 (MQ=255)                                                               gcTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACg                                                                                                                                                                                                                                     <  1:192812/297‑1 (MQ=255)                                                               aCTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACg                                                                                                                                                                                                                                     <  1:460945/298‑1 (MQ=255)                                                                        cttcttTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGTTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAAACACGACCGTTTTCACCGACAAAACCGACCGAGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCAGTATATCGAGAACACACCATCGGTGGca                                                                                                                                                                                                                                                                                 >  2:317225/1‑244 (MQ=255)                                                                                 gcgcCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCatat                                                                                                                                                                                                                 <  1:230515/300‑1 (MQ=255)                                                                                   gccgcGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACNTCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATc                                                                                                                                                                                                                <  1:515835/299‑1 (MQ=255)                                                                                         gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTCTCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCAGCTTCACACGCTTCTTTCAGACGTTCTTCTAGCTCCCGTATATCgttgtt                                                                                                                                                                                                          >  2:397275/1‑299 (MQ=255)                                                                                         gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCgag                                                                                                                                                                                                                                                                                                   <  1:696915/210‑1 (MQ=255)                                                                                         gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCgag                                                                                                                                                                                                                                                                                                   >  2:696915/1‑210 (MQ=255)                                                                                         gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCgttgtt                                                                                                                                                                                                          >  1:450790/1‑299 (MQ=255)                                                                                              accaccAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCCCCGACACAACCGCCCGCGTGTGCGTCGTCTACCATCACCCGGGTATCGTATTTATCTGCCAGATCGCCAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCAGTGGCAATCAACACATGCCGCGCACCGGCTTAACGCGCTTATTTCAGACGTACTTCCAGCTCCTGCCTCTAGTTGTTGGCata                                                                                                                                                                                                    >  2:701040/1‑300 (MQ=255)                                                                                                        gCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGt                                                                                                                                                                                          >  1:650002/1‑300 (MQ=255)                                                                                                        gCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGAAGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGt                                                                                                                                                                                          >  1:704863/1‑300 (MQ=255)                                                                                                                    gcTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTacac                                                                                                                                                                              <  2:409791/300‑1 (MQ=255)                                                                                                                        tACCAAGTGTGCCGTTGATAATATCGACCCTGCCCATCACATCGCAGTATTCATTGGAACCACGACCGTTTTCACCTACAAAACCGACCTCGTGTGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCACAAACGCCCTTCAGATTGGCAAGCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGa                                                                                                                                                                           <  2:354283/299‑1 (MQ=255)                                                                                                                           ccagtgtgccGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGa                                                                                                                                                                                                                                                                     >  2:648370/9‑206 (MQ=255)                                                                                                                           caagtgtgccGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTacac                                                                                                                                                                              <  1:648370/285‑1 (MQ=255)                                                                                                                              gtgtgccGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCacac                                                                                                                                                                    <  1:414905/295‑1 (MQ=255)                                                                                                                                gtgccGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCa                                                                                                                                                                  >  1:330522/4‑300 (MQ=255)                                                                                                                                        gATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACNATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCaataat                                                                                                                                                          <  2:546320/300‑1 (MQ=255)                                                                                                                                        gATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCaataat                                                                                                                                                          <  2:303804/300‑1 (MQ=255)                                                                                                                                                          cATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGTGCTTCTTTCAGGCGTGCTTACAGCTCCTGCATATCGTTGTTGGTATAGCGATAGCGTTTAGCGTTAAACAGACGcaaatc                                                                                                                                                                   >  2:391855/1‑269 (MQ=255)                                                                                                                                                                   gCAGTCTTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCAACAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAAAACACCATCGGTGGCAATCAAAACATGANACGCACCAGCTTCAAGCGCCCCTTTAAGACATGCTCCCCGCTCATGCATGTAGTTGTTGGTATAGCGATAGCGTTCAGCGTTAAACAGAAGAACGCTATCAATAATCGGAGAGTGGTTCAGTGCGTCGaaga                                                                                                                                >  2:217211/1‑299 (MQ=255)                                                                                                                                                                       tATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGTGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAAt                                                                                                                            <  2:444675/299‑1 (MQ=255)                                                                                                                                                                        aTTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAAt                                                                                                                            >  1:90395/1‑298 (MQ=255)                                                                                                                                                                                     aCGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGcgc                                                                                                              <  2:599104/299‑3 (MQ=255)                                                                                                                                                                                                     aCAAAACCGACCGCGTGGGAGTCGTCTACCATCACCATTGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGGCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAAc                                                                                             <  2:602162/300‑1 (MQ=255)                                                                                                                                                                                                     aCAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGTTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAAc                                                                                             <  1:458388/300‑1 (MQ=255)                                                                                                                                                                                                     aCAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAAc                                                                                             <  1:458378/300‑1 (MQ=255)                                                                                                                                                                                                     aCAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGGCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAAc                                                                                             <  2:602133/300‑1 (MQ=255)                                                                                                                                                                                                                          tcgtcTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAg                                                                        <  1:239956/300‑1 (MQ=255)                                                                                                                                                                                                                           cgtcTACCATCAACAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGc                                                                       >  1:178817/1‑300 (MQ=255)                                                                                                                                                                                                                                aCCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCAACGTTATCATCCAAGCa                                                                      >  2:694068/1‑296 (MQ=255)                                                                                                                                                                                                                                   aTCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCCAAGCAGGAaga                                                                >  1:701638/1‑299 (MQ=255)                                                                                                                                                                                                                                     cACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTa                                                             >  1:189019/1‑300 (MQ=255)                                                                                                                                                                                                                                       ccAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTaga                                                           >  1:12701/1‑300 (MQ=255)                                                                                                                                                                                                                                       ccAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCAACGTTAGCATCAAAGCAGGAAGAGTaga                                                           >  1:420994/1‑300 (MQ=255)                                                                                                                                                                                                                                       ccAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTCCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTaga                                                           >  1:13874/1‑300 (MQ=255)                                                                                                                                                                                                                                         aGGGCATCGTCTTTATCTGCCAGACCGCAAACGCCCTTCAGATTTACAAGCACGCCGGCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTagag                                                          <  2:266323/299‑1 (MQ=255)                                                                                                                                                                                                                                         aGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTagag                                                          <  2:266835/299‑1 (MQ=255)                                                                                                                                                                                                                                          gggCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGaa                                                        >  1:571097/1‑300 (MQ=255)                                                                                                                                                                                                                                           ggCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAt                                                       <  1:391855/300‑1 (MQ=255)                                                                                                                                                                                                                                           ggCATCGTATTTATCTGCCAAATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATNGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAt                                                       <  2:501658/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gtatTTATCTGCCGGATCACAAACGCCGTTCAGATTGGCAATCACTCCGTCCATCGAGAACACACCACCGGTAACAATCAAAACATAACGCACACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTTCTGCATATCGCTGTTGGCATAACTATAGCGTTTAGCTTTACACAGATGAACACCGTCAATAATAGACGAGTGGTTCAGTGTGTCGGAGATAATGGCGTCTTCAGCGCCCAGCAGCGTTTCAAATAGGCCGCCGTTAGCATCAAAGCAGGACGAGTAGAGAATCGCATc                                                 >  1:334033/2‑300 (MQ=255)                                                                                                                                                                                                                                                 gtatTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACTCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATc                                                 >  1:334026/2‑300 (MQ=255)                                                                                                                                                                                                                                                 gtatTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATc                                                 >  1:490118/2‑300 (MQ=255)                                                                                                                                                                                                                                                 gtatTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAACCGCATc                                                 >  1:334718/2‑300 (MQ=255)                                                                                                                                                                                                                                                          tGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATTACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATccc                                        <  1:216607/300‑1 (MQ=255)                                                                                                                                                                                                                                                          tGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATccc                                        <  1:114190/300‑1 (MQ=255)                                                                                                                                                                                                                                                              aGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAgg                                    <  2:632511/300‑1 (MQ=255)                                                                                                                                                                                                                                                                aTCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGaa                                  <  1:115864/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 ggccATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTTGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACACGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGc  <  2:115569/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCTGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGc  <  1:84646/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTTGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGc  <  2:473787/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACATGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGc  <  2:265783/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGc  <  1:84861/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGc  <  2:450790/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     GGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTTATCTGCCAGATCGCAAACGCCCTTCAGGTTGGCAATCACGCCGTCCATTGAGAACACACCATCGGTGGCGATCAGCACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTGCACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGC  >  NC_000913/3791703‑3792289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑