Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,889	A→G	N221N (AAT→AAC)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,889	0	A	G	100.0%	159.6 / NA	48	N221N (AAT→AAC)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/19);  total (29/19)

CCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTTATCTGCCAGATCGCAAACGCCCTTCAGGTTGGCAATCACGCCGTCCATTGAGAACACACCATCGGTGGCGATCAGCACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTGCACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCAC  >  NC_000913/3791606‑3792181                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                     ccgccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCAAATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAac                                                                                                                                                                                                                                                                                      >  1:354283/1‑300 (MQ=255) ccgccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTATTTGCGCGCCGCGGTATAAACACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGCTAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGCACCACGACCGTTTTCACCGACaacac                                                                                                                                                                                                                                                                                      >  2:84861/1‑297 (MQ=255) ccgcagACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCCGCACTTTCATGGAAGCGGCGACCATAGCCGGTGCCAGCGAGTCGGAGAACAGGTACGGACGAGAACGTTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACAACCAGAAGCCCAGCCCAGCGCTTTACCAAGTGTGCCGGTGATGATATCGACCCGGCACATCACATCGCCGTATTCATGGGACCCACGACCGTTTTCCCCAACaaacc                                                                                                                                                                                                                                                                                      >  2:84646/1‑298 (MQ=255)       aCATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCCGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAACGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTAAACCACTTCNTTGCGCGCAGCGGTATAACCAACAGAAGCCCAGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACCTCGCAGTCTTCATGGGAACCACGACCGTTTTCGCCGACAAAacagacc                                                                                                                                                                                                                                                                                 >  2:216607/1‑299 (MQ=255)               tCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGNAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAg                                                                                                                                                                                                                                                                        <  1:130804/300‑1 (MQ=255)                    gAACTGACGCGCGTTCNCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATGGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACNGTTTTCACCGACAAAACCNACCGCGTGGGAgtcgtc                                                                                                                                                                                                                                                                   >  1:599104/1‑300 (MQ=255)                                    gCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGc                                                                                                                                                                                                                                                    <  1:461279/299‑1 (MQ=255)                                    gCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGca                                                                                                                                                                                                                                                   >  1:473787/1‑300 (MQ=255)                                             cGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTa                                                                                                                                                                                                                                          >  1:413004/1‑300 (MQ=255)                                             cGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTANTCATGGGAACCACGAACGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTa                                                                                                                                                                                                                                          >  1:128508/1‑300 (MQ=255)                                                                           aTCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGTTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCTTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAgagt                                                                                                                                                                                                             <  1:637987/299‑4 (MQ=255)                                                                             ctcCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAANACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGc                                                                                                                                                                                                          >  1:151278/1‑300 (MQ=255)                                                                             ctcCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGc                                                                                                                                                                                                          >  1:115569/1‑300 (MQ=255)                                                                                     cTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCAACAGAAGCCCCGCCCAGCGCTCTACCAAGTGTGCCGGTGATAATATCGAACCGGCCCATCACATCACAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAAACGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCACACGCCCTTCAGATTGGCAATCAc                                                                                                                                                                                                    >  2:114190/1‑298 (MQ=255)                                                                                                  ggcgacaaTAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGCCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGaa                                                                                                                                                                                     >  1:402127/5‑300 (MQ=255)                                                                                                      acaaTAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAacaca                                                                                                                                                                                 >  1:632511/1‑300 (MQ=255)                                                                                                               ggTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGTTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGANCCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACACCCTTCAGATTGGCAAGTACGCCGGCCATCGAGAACACACCATCGGTg                                                                                                                                                                        <  2:497484/300‑1 (MQ=255)                                                                                                                gTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACAAGGGCATCGTATTTATCTGCCCGATCGCAAACGCCCTTCAGATTGGCCATCACGCCGTCCATCGAGAACACACCATCGGt                                                                                                                                                                         >  2:328198/1‑298 (MQ=255)                                                                                                                                gaACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGAcg                                                                                                                                                       <  1:121647/300‑1 (MQ=255)                                                                                                                                     ggTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCTGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCAc                                                                                                                                                  >  1:265783/1‑300 (MQ=255)                                                                                                                                           gccGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCCTCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCtt                                                                                                                                            >  1:266323/3‑300 (MQ=255)                                                                                                                                           gACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCtt                                                                                                                                            >  1:266835/1‑300 (MQ=255)                                                                                                                                           gACGAGAACGCGGGCGCAGCCACTCAACCATTTCTTTGCGCGCCGCTGGATAACCACCAGAATCCCCGCCCAGCGCTCTACCAAGTTTGCCGTTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTCCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTCATCTTCCAGATCACAAACGCCCTCCAGGTTTGCGAGCACGCCGGCCACCGAGAACACACCATCGGTGGCAATcctgtctcttatacacatctcgc                                                                                                                                            <  2:160035/300‑23 (MQ=255)                                                                                                                                                                gcTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACg                                                                                                                         <  1:192812/297‑1 (MQ=255)                                                                                                                                                                aCTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACg                                                                                                                         <  1:460945/298‑1 (MQ=255)                                                                                                                                                                         cttcttTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGTTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAAACACGACCGTTTTCACCGACAAAACCGACCGAGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCAGTATATCGAGAACACACCATCGGTGGca                                                                                                                                                                     >  2:317225/1‑244 (MQ=255)                                                                                                                                                                                  gcgcCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCatat                                                                                                     <  1:230515/300‑1 (MQ=255)                                                                                                                                                                                    gccgcGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACNTCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATc                                                                                                    <  1:515835/299‑1 (MQ=255)                                                                                                                                                                                          gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTCTCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCAGCTTCACACGCTTCTTTCAGACGTTCTTCTAGCTCCCGTATATCgttgtt                                                                                              >  2:397275/1‑299 (MQ=255)                                                                                                                                                                                          gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCgag                                                                                                                                                                                       <  1:696915/210‑1 (MQ=255)                                                                                                                                                                                          gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCgag                                                                                                                                                                                       >  2:696915/1‑210 (MQ=255)                                                                                                                                                                                          gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCgttgtt                                                                                              >  1:450790/1‑299 (MQ=255)                                                                                                                                                                                               accaccAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCCCCGACACAACCGCCCGCGTGTGCGTCGTCTACCATCACCCGGGTATCGTATTTATCTGCCAGATCGCCAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCAGTGGCAATCAACACATGCCGCGCACCGGCTTAACGCGCTTATTTCAGACGTACTTCCAGCTCCTGCCTCTAGTTGTTGGCata                                                                                        >  2:701040/1‑300 (MQ=255)                                                                                                                                                                                                         gCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGt                                                                              >  1:650002/1‑300 (MQ=255)                                                                                                                                                                                                         gCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGAAGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGt                                                                              >  1:704863/1‑300 (MQ=255)                                                                                                                                                                                                                     gcTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTacac                                                                  <  2:409791/300‑1 (MQ=255)                                                                                                                                                                                                                         tACCAAGTGTGCCGTTGATAATATCGACCCTGCCCATCACATCGCAGTATTCATTGGAACCACGACCGTTTTCACCTACAAAACCGACCTCGTGTGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCACAAACGCCCTTCAGATTGGCAAGCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGa                                                               <  2:354283/299‑1 (MQ=255)                                                                                                                                                                                                                            ccagtgtgccGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGa                                                                                                                                                         >  2:648370/9‑206 (MQ=255)                                                                                                                                                                                                                            caagtgtgccGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTacac                                                                  <  1:648370/285‑1 (MQ=255)                                                                                                                                                                                                                               gtgtgccGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCacac                                                        <  1:414905/295‑1 (MQ=255)                                                                                                                                                                                                                                 gtgccGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCa                                                      >  1:330522/4‑300 (MQ=255)                                                                                                                                                                                                                                         gATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACNATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCaataat                                              <  2:546320/300‑1 (MQ=255)                                                                                                                                                                                                                                         gATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCaataat                                              <  2:303804/300‑1 (MQ=255)                                                                                                                                                                                                                                                           cATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGTGCTTCTTTCAGGCGTGCTTACAGCTCCTGCATATCGTTGTTGGTATAGCGATAGCGTTTAGCGTTAAACAGACGcaaatc                                                       >  2:391855/1‑269 (MQ=255)                                                                                                                                                                                                                                                                    gCAGTCTTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCAACAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAAAACACCATCGGTGGCAATCAAAACATGANACGCACCAGCTTCAAGCGCCCCTTTAAGACATGCTCCCCGCTCATGCATGTAGTTGTTGGTATAGCGATAGCGTTCAGCGTTAAACAGAAGAACGCTATCAATAATCGGAGAGTGGTTCAGTGCGTCGaaga                    >  2:217211/1‑299 (MQ=255)                                                                                                                                                                                                                                                                        tATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGTGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAAt                <  2:444675/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         aTTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAAt                >  1:90395/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                      aCGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGcgc  <  2:599104/299‑3 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                     CCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTTATCTGCCAGATCGCAAACGCCCTTCAGGTTGGCAATCACGCCGTCCATTGAGAACACACCATCGGTGGCGATCAGCACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTGCACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCAC  >  NC_000913/3791606‑3792181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑