Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,829	C→T	T241T (ACG→ACA)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,829	0	C	T	100.0%	186.9 / NA	56	T241T (ACG→ACA)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/22);  total (34/22)

CTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTTATCTGCCAGATCGCAAACGCCCTTCAGGTTGGCAATCACGCCGTCCATTGAGAACACACCATCGGTGGCGATCAGCACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTGCACAGA  >  NC_000913/3791543‑3792120                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                    cTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGataa                                                                                                                                                                                                                                                                                        >  1:409791/1‑300 (MQ=255)          gcgagatgtgtataagagacagggCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGAccc                                                                                                                                                                                                                                                                               >  1:602133/23‑300 (MQ=255)          gcgagatgtgtataagagacagggCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAAAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGaacc                                                                                                                                                                                                                                                                               >  1:602162/23‑297 (MQ=255)           gccAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGAccc                                                                                                                                                                                                                                                                               >  1:546320/2‑299 (MQ=255)              aaGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTNTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGcc                                                                                                                                                                                                                                                                           >  1:501658/1‑300 (MQ=255)                    gACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTNGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCGGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                                                                                                                                                                                                                     <  1:515719/300‑1 (MQ=255)                    gACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTNGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                                                                                                                                                                                                                     <  1:515728/300‑1 (MQ=255)                    gACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                                                                                                                                                                                                                     <  1:682250/300‑1 (MQ=255)                    gACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCACGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                                                                                                                                                                                                                     <  1:652167/300‑1 (MQ=255)                         gAATACTGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAAACAACAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATTGACCCGGCCCATCAAATCGc                                                                                                                                                                                                                                                                >  2:458388/1‑300 (MQ=255)                         gAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCAGCACAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGc                                                                                                                                                                                                                                                                >  2:458378/1‑300 (MQ=255)                           ataatGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGCCATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCAGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTCCCAAGTGTGCCGGTGATAATATCGACCAGGCCCATCACAt                                                                                                                                                                                                                                                                   >  2:115864/1‑295 (MQ=255)                                ggCGTGATTGGCTCCCGCCAGGGGAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTGCAACCATCTCCAGCACTTTAATGTAAGCGTCGACAAGAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAATGTGCCGGTGATAACATCGACCCGGCCCATCACATCGCAGTATTc                                                                                                                                                                                                                                                         <  2:340375/300‑1 (MQ=255)                                 gCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCATTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCACCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTc                                                                                                                                                                                                                                                         <  2:47973/299‑1 (MQ=255)                                   ttGATCGGCGCCCGCCAGGTTAAAGCCCGCCGCCGACATTGGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAAAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCATAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCACCACATCGCAGTATTCATg                                                                                                                                                                                                                                                      <  2:321433/299‑1 (MQ=255)                                   gTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCGACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAACCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATg                                                                                                                                                                                                                                                      <  2:320568/300‑1 (MQ=255)                                    tGATCTGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATg                                                                                                                                                                                                                                                      <  2:321531/299‑1 (MQ=255)                                          gCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCa                                                                                                                                                                                                                                               >  1:444675/1‑300 (MQ=255)                                          gCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCAACAGAAGCACCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCa                                                                                                                                                                                                                                               >  2:239956/1‑300 (MQ=255)                                                                ccgccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCAAATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAac                                                                                                                                                                                                                         >  1:354283/1‑300 (MQ=255)                                                                ccgccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTATTTGCGCGCCGCGGTATAAACACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGCTAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGCACCACGACCGTTTTCACCGACaacac                                                                                                                                                                                                                         >  2:84861/1‑297 (MQ=255)                                                                ccgcagACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCCGCACTTTCATGGAAGCGGCGACCATAGCCGGTGCCAGCGAGTCGGAGAACAGGTACGGACGAGAACGTTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACAACCAGAAGCCCAGCCCAGCGCTTTACCAAGTGTGCCGGTGATGATATCGACCCGGCACATCACATCGCCGTATTCATGGGACCCACGACCGTTTTCCCCAACaaacc                                                                                                                                                                                                                         >  2:84646/1‑298 (MQ=255)                                                                      aCATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCCGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAACGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTAAACCACTTCNTTGCGCGCAGCGGTATAACCAACAGAAGCCCAGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACCTCGCAGTCTTCATGGGAACCACGACCGTTTTCGCCGACAAAacagacc                                                                                                                                                                                                                    >  2:216607/1‑299 (MQ=255)                                                                              tCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGNAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAg                                                                                                                                                                                                           <  1:130804/300‑1 (MQ=255)                                                                                   gAACTGACGCGCGTTCNCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATGGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACNGTTTTCACCGACAAAACCNACCGCGTGGGAgtcgtc                                                                                                                                                                                                      >  1:599104/1‑300 (MQ=255)                                                                                                   gCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGc                                                                                                                                                                                       <  1:461279/299‑1 (MQ=255)                                                                                                   gCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGca                                                                                                                                                                                      >  1:473787/1‑300 (MQ=255)                                                                                                            cGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTa                                                                                                                                                                             >  1:413004/1‑300 (MQ=255)                                                                                                            cGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTANTCATGGGAACCACGAACGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTa                                                                                                                                                                             >  1:128508/1‑300 (MQ=255)                                                                                                                                          aTCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGTTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCTTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAgagt                                                                                                                                                <  1:637987/299‑4 (MQ=255)                                                                                                                                            ctcCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAANACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGc                                                                                                                                             >  1:151278/1‑300 (MQ=255)                                                                                                                                            ctcCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGc                                                                                                                                             >  1:115569/1‑300 (MQ=255)                                                                                                                                                    cTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCAACAGAAGCCCCGCCCAGCGCTCTACCAAGTGTGCCGGTGATAATATCGAACCGGCCCATCACATCACAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAAACGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCACACGCCCTTCAGATTGGCAATCAc                                                                                                                                       >  2:114190/1‑298 (MQ=255)                                                                                                                                                                 ggcgacaaTAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGCCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGaa                                                                                                                        >  1:402127/5‑300 (MQ=255)                                                                                                                                                                     acaaTAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAacaca                                                                                                                    >  1:632511/1‑300 (MQ=255)                                                                                                                                                                              ggTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGTTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGANCCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACACCCTTCAGATTGGCAAGTACGCCGGCCATCGAGAACACACCATCGGTg                                                                                                           <  2:497484/300‑1 (MQ=255)                                                                                                                                                                               gTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACAAGGGCATCGTATTTATCTGCCCGATCGCAAACGCCCTTCAGATTGGCCATCACGCCGTCCATCGAGAACACACCATCGGt                                                                                                            >  2:328198/1‑298 (MQ=255)                                                                                                                                                                                               gaACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGAcg                                                                                          <  1:121647/300‑1 (MQ=255)                                                                                                                                                                                                    ggTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCTGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCAc                                                                                     >  1:265783/1‑300 (MQ=255)                                                                                                                                                                                                          gccGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCCTCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCtt                                                                               >  1:266323/3‑300 (MQ=255)                                                                                                                                                                                                          gACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCtt                                                                               >  1:266835/1‑300 (MQ=255)                                                                                                                                                                                                          gACGAGAACGCGGGCGCAGCCACTCAACCATTTCTTTGCGCGCCGCTGGATAACCACCAGAATCCCCGCCCAGCGCTCTACCAAGTTTGCCGTTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTCCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTCATCTTCCAGATCACAAACGCCCTCCAGGTTTGCGAGCACGCCGGCCACCGAGAACACACCATCGGTGGCAATcctgtctcttatacacatctcgc                                                                               <  2:160035/300‑23 (MQ=255)                                                                                                                                                                                                                               gcTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACg                                                            <  1:192812/297‑1 (MQ=255)                                                                                                                                                                                                                               aCTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACg                                                            <  1:460945/298‑1 (MQ=255)                                                                                                                                                                                                                                        cttcttTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGTTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAAACACGACCGTTTTCACCGACAAAACCGACCGAGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCAGTATATCGAGAACACACCATCGGTGGca                                                                                                        >  2:317225/1‑244 (MQ=255)                                                                                                                                                                                                                                                 gcgcCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCatat                                        <  1:230515/300‑1 (MQ=255)                                                                                                                                                                                                                                                   gccgcGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACNTCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATc                                       <  1:515835/299‑1 (MQ=255)                                                                                                                                                                                                                                                         gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTCTCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCAGCTTCACACGCTTCTTTCAGACGTTCTTCTAGCTCCCGTATATCgttgtt                                 >  2:397275/1‑299 (MQ=255)                                                                                                                                                                                                                                                         gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCgag                                                                                                                          >  2:696915/1‑210 (MQ=255)                                                                                                                                                                                                                                                         gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCgag                                                                                                                          <  1:696915/210‑1 (MQ=255)                                                                                                                                                                                                                                                         gTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCgttgtt                                 >  1:450790/1‑299 (MQ=255)                                                                                                                                                                                                                                                              accaccAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCCCCGACACAACCGCCCGCGTGTGCGTCGTCTACCATCACCCGGGTATCGTATTTATCTGCCAGATCGCCAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCAGTGGCAATCAACACATGCCGCGCACCGGCTTAACGCGCTTATTTCAGACGTACTTCCAGCTCCTGCCTCTAGTTGTTGGCata                           >  2:701040/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGt                 >  1:650002/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGAAGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGt                 >  1:704863/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gcTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGCAATCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTacac     <  2:409791/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        tACCAAGTGTGCCGTTGATAATATCGACCCTGCCCATCACATCGCAGTATTCATTGGAACCACGACCGTTTTCACCTACAAAACCGACCTCGTGTGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCACAAACGCCCTTCAGATTGGCAAGCACGCCGTCCATCGAGAACACACCATCGGTGGCAATCAACACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTACACAGa  <  2:354283/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                    CTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTTATCTGCCAGATCGCAAACGCCCTTCAGGTTGGCAATCACGCCGTCCATTGAGAACACACCATCGGTGGCGATCAGCACATGACGCGCACCGGCTTCACGCGCTTCTTTCAGACGTGCTTCCAGCTCCTGCATATCGTTGTTGGCATAGCGATAGCGTTTAGCTTTGCACAGA  >  NC_000913/3791543‑3792120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑