Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,706	A→G	V282V (GTT→GTC)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,706	0	A	G	100.0%	182.8 / NA	51	V282V (GTT→GTC)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/26);  total (25/26)

TTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTTATCTGCCAGATCGCAAACGCCCTTCAGGTTGGCAATCAC  >  NC_000913/3791415‑3791987                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                          ttGCTCATGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGTATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAAACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAAtagc                                                                                                                                                                                                                                                                                   <  2:41751/300‑4 (MQ=255) ttGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCTGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCTACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAAtagc                                                                                                                                                                                                                                                                                   <  2:41985/300‑4 (MQ=255)   acTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATATAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCAGCTCACGCAAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGcc                                                                                                                                                                                                                                                                                  >  2:230515/2‑299 (MQ=255)   acTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAACCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGcc                                                                                                                                                                                                                                                                                  >  2:414905/2‑299 (MQ=255)                               ggTACGAATACGCGCCTNACCTTTCGGAACGACCGGNTAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCGCGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGNTGGAGAACAGGTACgg                                                                                                                                                                                                                                                     <  1:598308/300‑1 (MQ=255)                                gtataaaTACGCGACTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAACTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATTGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGTTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGa                                                                                                                                                                                                                                                    <  2:156476/295‑1 (MQ=255)                                gTACGAATACGCGCCTGACCTTTCGGCACGAACGGATAGAGGACACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCAAGGGTAAAGCCCGCCGCAGACATTTGCTCAAGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGa                                                                                                                                                                                                                                                    >  2:192812/1‑300 (MQ=255)                                gTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGa                                                                                                                                                                                                                                                    <  2:425810/300‑1 (MQ=255)                                       tACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGGCAGCGAGTTGGAGAACAGGTACGGACGAGAAc                                                                                                                                                                                                                                             <  1:488891/300‑1 (MQ=255)                                                                 ggATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTCAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCActtct                                                                                                                                                                                                                   >  2:156655/1‑300 (MQ=255)                                                                       aagaaACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTgcgc                                                                                                                                                                                                             <  2:171958/300‑1 (MQ=255)                                                                                     gTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATTGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGTTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGTAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAacc                                                                                                                                                                                               <  2:249786/300‑1 (MQ=255)                                                                                      tAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGGGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAacca                                                                                                                                                                                              <  2:293071/300‑1 (MQ=255)                                                                                           gCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGACCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGa                                                                                                                                                                                         <  1:88334/300‑1 (MQ=255)                                                                                               tctTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGGAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGc                                                                                                                                                                                      <  2:285930/299‑1 (MQ=255)                                                                                                 tttttGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGccc                                                                                                                                                                                    <  1:41720/299‑1 (MQ=255)                                                                                                     tGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGccc                                                                                                                                                                               <  1:156655/300‑1 (MQ=255)                                                                                                                 gCAAATTTCTGCGCCACTACCGCAGCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCACGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCa                                                                                                                                                                   <  2:312163/300‑1 (MQ=255)                                                                                                                                 cTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGataa                                                                                                                                                   >  1:409791/1‑300 (MQ=255)                                                                                                                                          gcgagatgtgtataagagacagggCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGAccc                                                                                                                                          >  1:602133/23‑300 (MQ=255)                                                                                                                                          gcgagatgtgtataagagacagggCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAAAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGaacc                                                                                                                                          >  1:602162/23‑297 (MQ=255)                                                                                                                                           gccAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGAccc                                                                                                                                          >  1:546320/2‑299 (MQ=255)                                                                                                                                              aaGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTNTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGcc                                                                                                                                      >  1:501658/1‑300 (MQ=255)                                                                                                                                                    gACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTNGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCGGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                                                                                <  1:515719/300‑1 (MQ=255)                                                                                                                                                    gACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTNGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                                                                                <  1:515728/300‑1 (MQ=255)                                                                                                                                                    gACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                                                                                <  1:682250/300‑1 (MQ=255)                                                                                                                                                    gACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCACGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                                                                                <  1:652167/300‑1 (MQ=255)                                                                                                                                                         gAATACTGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAAACAACAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATTGACCCGGCCCATCAAATCGc                                                                                                                           >  2:458388/1‑300 (MQ=255)                                                                                                                                                         gAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCAGCACAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGc                                                                                                                           >  2:458378/1‑300 (MQ=255)                                                                                                                                                           ataatGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGCCATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCAGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTCCCAAGTGTGCCGGTGATAATATCGACCAGGCCCATCACAt                                                                                                                              >  2:115864/1‑295 (MQ=255)                                                                                                                                                                ggCGTGATTGGCTCCCGCCAGGGGAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTGCAACCATCTCCAGCACTTTAATGTAAGCGTCGACAAGAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAATGTGCCGGTGATAACATCGACCCGGCCCATCACATCGCAGTATTc                                                                                                                    <  2:340375/300‑1 (MQ=255)                                                                                                                                                                 gCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCATTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCACCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTc                                                                                                                    <  2:47973/299‑1 (MQ=255)                                                                                                                                                                   ttGATCGGCGCCCGCCAGGTTAAAGCCCGCCGCCGACATTGGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAAAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCATAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCACCACATCGCAGTATTCATg                                                                                                                 <  2:321433/299‑1 (MQ=255)                                                                                                                                                                   gTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCGACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAACCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATg                                                                                                                 <  2:320568/300‑1 (MQ=255)                                                                                                                                                                    tGATCTGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATg                                                                                                                 <  2:321531/299‑1 (MQ=255)                                                                                                                                                                          gCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCa                                                                                                          >  1:444675/1‑300 (MQ=255)                                                                                                                                                                          gCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCAACAGAAGCACCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCa                                                                                                          >  2:239956/1‑300 (MQ=255)                                                                                                                                                                                                ccgccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCAAATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAac                                                                                    >  1:354283/1‑300 (MQ=255)                                                                                                                                                                                                ccgccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTATTTGCGCGCCGCGGTATAAACACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGCTAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGCACCACGACCGTTTTCACCGACaacac                                                                                    >  2:84861/1‑297 (MQ=255)                                                                                                                                                                                                ccgcagACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCCGCACTTTCATGGAAGCGGCGACCATAGCCGGTGCCAGCGAGTCGGAGAACAGGTACGGACGAGAACGTTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACAACCAGAAGCCCAGCCCAGCGCTTTACCAAGTGTGCCGGTGATGATATCGACCCGGCACATCACATCGCCGTATTCATGGGACCCACGACCGTTTTCCCCAACaaacc                                                                                    >  2:84646/1‑298 (MQ=255)                                                                                                                                                                                                      aCATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCCGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAACGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTAAACCACTTCNTTGCGCGCAGCGGTATAACCAACAGAAGCCCAGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACCTCGCAGTCTTCATGGGAACCACGACCGTTTTCGCCGACAAAacagacc                                                                               >  2:216607/1‑299 (MQ=255)                                                                                                                                                                                                              tCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGNAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAg                                                                      <  1:130804/300‑1 (MQ=255)                                                                                                                                                                                                                   gAACTGACGCGCGTTCNCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATGGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACNGTTTTCACCGACAAAACCNACCGCGTGGGAgtcgtc                                                                 >  1:599104/1‑300 (MQ=255)                                                                                                                                                                                                                                   gCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGc                                                  <  1:461279/299‑1 (MQ=255)                                                                                                                                                                                                                                   gCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGca                                                 >  1:473787/1‑300 (MQ=255)                                                                                                                                                                                                                                            cGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTa                                        >  1:413004/1‑300 (MQ=255)                                                                                                                                                                                                                                            cGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTANTCATGGGAACCACGAACGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTa                                        >  1:128508/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          aTCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGTTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCTTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAgagt           <  1:637987/299‑4 (MQ=255)                                                                                                                                                                                                                                                                            ctcCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAANACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGc        >  1:151278/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            ctcCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCAAACGCCCTTCAGATTGGc        >  1:115569/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    cTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCAACAGAAGCCCCGCCCAGCGCTCTACCAAGTGTGCCGGTGATAATATCGAACCGGCCCATCACATCACAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAAACGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCGTATTTATCTGCCAGATCGCACACGCCCTTCAGATTGGCAATCAc  >  2:114190/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                          TTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGCATCATATTTATCTGCCAGATCGCAAACGCCCTTCAGGTTGGCAATCAC  >  NC_000913/3791415‑3791987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑