Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,553	A→G	G333G (GGT→GGC)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,553	0	A	G	100.0%	208.8 / NA	57	G333G (GGT→GGC)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/42);  total (15/42)

GCAGATCGTTATGCCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAA  >  NC_000913/3791261‑3791842                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                    gcagATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                                                                                                                                                                                                                              <  2:644346/300‑1 (MQ=255) gcagATCGGTATGACCCAGGTCCGGCTCAGGAATGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTGTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAANGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATATAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                                                                                                                                                                                                                              <  2:214910/300‑1 (MQ=255)              acccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGACTGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaataat                                                                                                                                                                                                                                                                                 >  2:652167/5‑300 (MQ=255)              acccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaataat                                                                                                                                                                                                                                                                                 >  2:130804/5‑300 (MQ=255)                  atttccggctcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATgg                                                                                                                                                                                                                                                                               <  1:575584/288‑1 (MQ=255)                  aGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATgg                                                                                                                                                                                                                                                                               <  1:61188/298‑1 (MQ=255)                   gTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGt                                                                                                                                                                                                                                                                            >  1:285930/1‑300 (MQ=255)                           tcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGc                                                                                                                                                                                                                                                                     <  2:256263/298‑1 (MQ=255)                             aGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTNCC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGATCGGAATAATGGCGTGATCGGCTcc                                                                                                                                                                                                                                                                  <  2:546466/300‑1 (MQ=255)                                 acggcGGTCATCCAGATGCCCTCTGCCGCTTGCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTGG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCGCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAACATGACCGGAATAATGGCGTGATCGGCTCCCGcc                                                                                                                                                                                                                                                              <  2:385285/296‑1 (MQ=255)                                 aCGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGcc                                                                                                                                                                                                                                                              <  1:626096/300‑1 (MQ=255)                                      ggTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGt                                                                                                                                                                                                                                                         <  1:383266/300‑1 (MQ=255)                                             cAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGNGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGccc                                                                                                                                                                                                                                                  >  1:497484/1‑300 (MQ=255)                                              aGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCATTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGcc                                                                                                                                                                                                                                                   <  2:106785/298‑1 (MQ=255)                                               gATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGCTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGACCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCccgc                                                                                                                                                                                                                                                >  1:340375/1‑300 (MQ=255)                                                                      tGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACt                                                                                                                                                                                                                       <  1:221111/300‑1 (MQ=255)                                                                                 tcattgtcACATCCTCANGGCGATAACGCCCAGTTGNT‑TTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                                                                                                                                                                                   <  1:581977/294‑1 (MQ=255)                                                                                 tcattgtcACATCCTCANGGCGATAACGCCCAGTTG‑NCTTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                                                                                                                                                                                   <  1:582003/294‑1 (MQ=255)                                                                                 tcatcgtcACATCCTCANGGCGATAACGCCCAGTTG‑NCTTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                                                                                                                                                                                   <  1:581985/294‑1 (MQ=255)                                                                                         acaTCCTCA‑TGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGGAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAg                                                                                                                                                                                                    <  1:669016/300‑1 (MQ=255)                                                                                         acaTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAg                                                                                                                                                                                                    <  1:666356/300‑1 (MQ=255)                                                                                           aTCCTCA‑GTCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTANGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGa                                                                                                                                                                                                   <  1:500780/299‑1 (MQ=255)                                                                                           aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGAc                                                                                                                                                                                                  <  2:139324/300‑1 (MQ=255)                                                                                           aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGAc                                                                                                                                                                                                  <  1:116769/300‑1 (MQ=255)                                                                                           aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTANGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGa                                                                                                                                                                                                   <  1:500649/299‑1 (MQ=255)                                                                                                    gCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGc                                                                                                                                                                                          <  2:140968/300‑1 (MQ=255)                                                                                                      gATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACAAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGc                                                                                                                                                                                          <  2:180412/298‑1 (MQ=255)                                                                                                            gCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTCCTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTANATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCACGCAGCCGACATTT‑‑GCTCACGGAACTGACGAGCGTTCGCCCACAGACGGTCACGCAGCTCGCt                                                                                                                                                                                  >  2:515835/1‑300 (MQ=255)                                                                                                            gCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCt                                                                                                                                                                                  <  2:51346/300‑1 (MQ=255)                                                                                                                 gttg‑t‑tTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ATGCGCCTGACCTTTAGGAACGCCCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATCTCTGCGCCACTACCGCATCGACAAGCACGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGATAAAGCCCGCCACCGAAATTT‑‑GCCCACGGAACTGAAGCGCGTTCGCACACAGACGGTCACGCAGCTCGCTGccc                                                                                                                                                                              >  2:41720/1‑299 (MQ=255)                                                                                                                  agttacttACC‑‑AATACGNCTGAATGCTTCTACTGCGNCCGTAATGTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTNACGCAGCTCGCTGccc                                                                                                                                                                              <  1:584891/294‑1 (MQ=255)                                                                                                                  agttacttACC‑‑AATACGNATGAATGCTTCTACTGCGNGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTNACGCAGCTCGCTGccc                                                                                                                                                                              <  1:584915/294‑1 (MQ=255)                                                                                                                        ttACC‑‑AATACGCGTGAATGCTTCTACTGCGGGTGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAACGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCAGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCAAGCCGCTCGCTGCCCGCTTca                                                                                                                                                                        >  2:121647/1‑299 (MQ=255)                                                                                                                               aaTACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCa                                                                                                                                                                    <  1:446411/299‑1 (MQ=255)                                                                                                                                   cgcgTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTATGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGACCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTcc                                                                                                                                                               >  2:460945/1‑300 (MQ=255)                                                                                                                                           tGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTCACGCGCGTTCGCCCACAGACGGTCAAGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACttt                                                                                                                                                       >  1:160035/1‑300 (MQ=255)                                                                                                                                                 tACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATgg                                                                                                                                                  <  1:71802/299‑1 (MQ=255)                                                                                                                                                 tACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATgg                                                                                                                                                  <  1:71867/299‑1 (MQ=255)                                                                                                                                                                ttGCTCATGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGTATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAAACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAAtagc                                                                                                                                  <  2:41751/300‑4 (MQ=255)                                                                                                                                                                ttGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCTGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCTACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAAtagc                                                                                                                                  <  2:41985/300‑4 (MQ=255)                                                                                                                                                                  acTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATATAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCAGCTCACGCAAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGcc                                                                                                                                 >  2:230515/2‑299 (MQ=255)                                                                                                                                                                  acTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAACCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGcc                                                                                                                                 >  2:414905/2‑299 (MQ=255)                                                                                                                                                                                              ggTACGAAT‑ACGCGCCTNACCTTTCGGAACGACCGGNTAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCGCGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGNTGGAGAACAGGTACgg                                                                                                    <  1:598308/300‑1 (MQ=255)                                                                                                                                                                                               gtataaaT‑ACGCGACTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAACTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATTGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGTTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGa                                                                                                   <  2:156476/295‑1 (MQ=255)                                                                                                                                                                                               gTACGAAT‑ACGCGCCTGACCTTTCGGCACGAACGGATAGAGGACACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCAAGGGTAAAGCCCGCCGCAGACATTT‑‑GCTCAAGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGa                                                                                                   >  2:192812/1‑300 (MQ=255)                                                                                                                                                                                               gTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGa                                                                                                   <  2:425810/300‑1 (MQ=255)                                                                                                                                                                                                      t‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGGCAGCGAGTTGGAGAACAGGTACGGACGAGAAc                                                                                            <  1:488891/300‑1 (MQ=255)                                                                                                                                                                                                                                 ggATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTCAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCActtct                                                                  >  2:156655/1‑300 (MQ=255)                                                                                                                                                                                                                                       aagaaACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTgcgc                                                            <  2:171958/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATTGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGTTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGTAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAacc                                              <  2:249786/300‑1 (MQ=255)                                                                                                                                                                                                                                                      tAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGGGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAacca                                             <  2:293071/300‑1 (MQ=255)                                                                                                                                                                                                                                                           gCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGACCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGa                                        <  1:88334/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tctTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGGAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGc                                     <  2:285930/299‑1 (MQ=255)                                                                                                                                                                                                                                                                 tttttGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGccc                                   <  1:41720/299‑1 (MQ=255)                                                                                                                                                                                                                                                                     tGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGccc                              <  1:156655/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gCAAATTTCTGCGCCACTACCGCAGCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCACGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCa                  <  2:312163/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 cTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGataa  >  1:409791/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                    GCAGATCGTTATGCCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAA  >  NC_000913/3791261‑3791842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑