BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,496	A→G	V352V (GTT→GTC)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,496	0	A	G	100.0%	220.3 / NA	63	V352V (GTT→GTC)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/43); total (20/43)

AGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATG‑CCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGC  >  NC_000913/3791207‑3791784

aGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAAt                                                                                                                                                                                                                                                                                            <  1:196276/298‑1 (MQ=255)

aGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGc                                                                                                                                                                                                                                                                                          <  1:448839/300‑1 (MQ=255)

  ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCNTTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGcac                                                                                                                                                                                                                                                                                        >  2:500649/4‑298 (MQ=255)

  ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCNTTTCACATCCTCA‑GGCGATAACGCACAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCAGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGACCGACCGGATAGAAGAAACCGGTGACGTAAATGcc                                                                                                                                                                                                                                                                                         >  2:500780/4‑299 (MQ=255)

  ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGAACTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGcac                                                                                                                                                                                                                                                                                        >  2:461279/4‑298 (MQ=255)

           tgAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGc                                                                                                                                                                                                                                                                               <  1:698290/300‑1 (MQ=255)

           tgAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGc                                                                                                                                                                                                                                                                               <  1:633193/300‑1 (MQ=255)

                                 cTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTgc                                                                                                                                                                                                                                                          >  1:171958/1‑299 (MQ=255)

                                          ggattgtaagtagcagATCTTTATGACCCAAGT‑CCGGCTCAGGAACGGCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGTGTTTATTTCACATCCTCA‑TGCGATAACGACCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAAGTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                                                                                                                                <  2:88665/289‑1 (MQ=255)

                                          ggATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GTCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                                                                                                                                <  1:529025/300‑1 (MQ=255)

                                          ggATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                                                                                                                                <  1:91810/300‑1 (MQ=255)

                                            aTTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCa                                                                                                                                                                                                                                              >  1:47973/1‑300 (MQ=255)

                                             ttttAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCAt                                                                                                                                                                                                                                             <  1:102423/300‑1 (MQ=255)

                                               ttAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAGATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCACCTACCGCATc                                                                                                                                                                                                                                            >  2:682250/1‑299 (MQ=255)

                                                      gcagATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                                                                                                                                                                    <  2:644346/300‑1 (MQ=255)

                                                      gcagATCGGTATG‑ACCCAGGTCCGGCTCAGGAATGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTGTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAANGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATATAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                                                                                                                                                                    <  2:214910/300‑1 (MQ=255)

                                                                    acccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGACTGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaataat                                                                                                                                                                                                                       >  2:652167/5‑300 (MQ=255)

                                                                    acccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaataat                                                                                                                                                                                                                       >  2:130804/5‑300 (MQ=255)

                                                                        atttccggctcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATgg                                                                                                                                                                                                                     <  1:575584/288‑1 (MQ=255)

                                                                        aGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATgg                                                                                                                                                                                                                     <  1:61188/298‑1 (MQ=255)

                                                                         gTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGt                                                                                                                                                                                                                  >  1:285930/1‑300 (MQ=255)

                                                                                 tcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGc                                                                                                                                                                                                           <  2:256263/298‑1 (MQ=255)

                                                                                   aGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTNCC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGATCGGAATAATGGCGTGATCGGCTcc                                                                                                                                                                                                        <  2:546466/300‑1 (MQ=255)

                                                                                       acggcGGTCATCCAGATGCCCTCTGCCGCTTGCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTGG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCGCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAACATGACCGGAATAATGGCGTGATCGGCTCCCGcc                                                                                                                                                                                                    <  2:385285/296‑1 (MQ=255)

                                                                                       aCGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGcc                                                                                                                                                                                                    <  1:626096/300‑1 (MQ=255)

                                                                                            ggTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGt                                                                                                                                                                                               <  1:383266/300‑1 (MQ=255)

                                                                                                   cAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGNGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGccc                                                                                                                                                                                        >  1:497484/1‑300 (MQ=255)

                                                                                                    aGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCATTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGcc                                                                                                                                                                                         <  2:106785/298‑1 (MQ=255)

                                                                                                     gATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGCTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGACCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCccgc                                                                                                                                                                                      >  1:340375/1‑300 (MQ=255)

                                                                                                                            tGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACt                                                                                                                                                             <  1:221111/300‑1 (MQ=255)

                                                                                                                                       tcattgtcACATCCTCANGGCGATAACGCCCAGTTGNT‑TTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                                                                                                                         <  1:581977/294‑1 (MQ=255)

                                                                                                                                       tcattgtcACATCCTCANGGCGATAACGCCCAGTTG‑NCTTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                                                                                                                         <  1:582003/294‑1 (MQ=255)

                                                                                                                                       tcatcgtcACATCCTCANGGCGATAACGCCCAGTTG‑NCTTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                                                                                                                         <  1:581985/294‑1 (MQ=255)

                                                                                                                                               acaTCCTCA‑TGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGGAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAg                                                                                                                                          <  1:669016/300‑1 (MQ=255)

                                                                                                                                               acaTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAg                                                                                                                                          <  1:666356/300‑1 (MQ=255)

                                                                                                                                                 aTCCTCA‑GTCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTANGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGa                                                                                                                                         <  1:500780/299‑1 (MQ=255)

                                                                                                                                                 aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGAc                                                                                                                                        <  1:116769/300‑1 (MQ=255)

                                                                                                                                                 aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGAc                                                                                                                                        <  2:139324/300‑1 (MQ=255)

                                                                                                                                                 aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTANGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGa                                                                                                                                         <  1:500649/299‑1 (MQ=255)

                                                                                                                                                          gCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGc                                                                                                                                <  2:140968/300‑1 (MQ=255)

                                                                                                                                                            gATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACAAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGc                                                                                                                                <  2:180412/298‑1 (MQ=255)

                                                                                                                                                                  gCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTCCTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTANATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCACGCAGCCGACATTT‑‑GCTCACGGAACTGACGAGCGTTCGCCCACAGACGGTCACGCAGCTCGCt                                                                                                                        >  2:515835/1‑300 (MQ=255)

                                                                                                                                                                  gCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCt                                                                                                                        <  2:51346/300‑1 (MQ=255)

                                                                                                                                                                       gttg‑t‑tTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ATGCGCCTGACCTTTAGGAACGCCCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATCTCTGCGCCACTACCGCATCGACAAGCACGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGATAAAGCCCGCCACCGAAATTT‑‑GCCCACGGAACTGAAGCGCGTTCGCACACAGACGGTCACGCAGCTCGCTGccc                                                                                                                    >  2:41720/1‑299 (MQ=255)

                                                                                                                                                                        agttacttACC‑‑AATACGNCTGAATGCTTCTACTGCGNCCGTAATGTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTNACGCAGCTCGCTGccc                                                                                                                    <  1:584891/294‑1 (MQ=255)

                                                                                                                                                                        agttacttACC‑‑AATACGNATGAATGCTTCTACTGCGNGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTNACGCAGCTCGCTGccc                                                                                                                    <  1:584915/294‑1 (MQ=255)

                                                                                                                                                                              ttACC‑‑AATACGCGTGAATGCTTCTACTGCGGGTGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAACGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCAGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCAAGCCGCTCGCTGCCCGCTTca                                                                                                              >  2:121647/1‑299 (MQ=255)

                                                                                                                                                                                     aaTACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCa                                                                                                          <  1:446411/299‑1 (MQ=255)

                                                                                                                                                                                         cgcgTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTATGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGACCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTcc                                                                                                     >  2:460945/1‑300 (MQ=255)

                                                                                                                                                                                                 tGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTCACGCGCGTTCGCCCACAGACGGTCAAGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACttt                                                                                             >  1:160035/1‑300 (MQ=255)

                                                                                                                                                                                                       tACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATgg                                                                                        <  1:71802/299‑1 (MQ=255)

                                                                                                                                                                                                       tACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATgg                                                                                        <  1:71867/299‑1 (MQ=255)

                                                                                                                                                                                                                      ttGCTCATGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGTATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAAACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAAtagc                                                                        <  2:41751/300‑4 (MQ=255)

                                                                                                                                                                                                                      ttGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCTGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCTACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAAtagc                                                                        <  2:41985/300‑4 (MQ=255)

                                                                                                                                                                                                                        acTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATATAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCAGCTCACGCAAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGcc                                                                       >  2:230515/2‑299 (MQ=255)

                                                                                                                                                                                                                        acTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAACCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGcc                                                                       >  2:414905/2‑299 (MQ=255)

                                                                                                                                                                                                                                                    ggTACGAAT‑ACGCGCCTNACCTTTCGGAACGACCGGNTAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCGCGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGNTGGAGAACAGGTACgg                                          <  1:598308/300‑1 (MQ=255)

                                                                                                                                                                                                                                                     gtataaaT‑ACGCGACTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAACTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATTGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGTTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGa                                         <  2:156476/295‑1 (MQ=255)

                                                                                                                                                                                                                                                     gTACGAAT‑ACGCGCCTGACCTTTCGGCACGAACGGATAGAGGACACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCAAGGGTAAAGCCCGCCGCAGACATTT‑‑GCTCAAGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGa                                         >  2:192812/1‑300 (MQ=255)

                                                                                                                                                                                                                                                     gTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGa                                         <  2:425810/300‑1 (MQ=255)

                                                                                                                                                                                                                                                            t‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGGCAGCGAGTTGGAGAACAGGTACGGACGAGAAc                                  <  1:488891/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                       ggATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTCAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTACAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCActtct        >  2:156655/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                             aagaaACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTgcgc  <  2:171958/300‑1 (MQ=255)

AGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATG‑CCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGC  >  NC_000913/3791207‑3791784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`