Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,406	A→G	R382R (CGT→CGC)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,406	0	A	G	100.0%	265.9 / NA	75	R382R (CGT→CGC)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/47);  total (28/47)

CTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATG‑CCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGG  >  NC_000913/3791120‑3791698                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                        cTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCt                                                                                                                                                                                                                                                                                            >  2:633193/1‑300 (MQ=255)       gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATTGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCTGCTCATGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                                                                                                                                                                                                                                      <  2:462397/292‑1 (MQ=255)       gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                                                                                                                                                                                                                                      <  1:422338/292‑1 (MQ=255)        accgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGNAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAGTTTGCTCAGGGGt                                                                                                                                                                                                                                                                                     >  2:515719/8‑300 (MQ=255)        accgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGNAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGt                                                                                                                                                                                                                                                                                     >  2:515728/8‑300 (MQ=255)            atgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                                                                                                                                                                 <  1:349063/297‑1 (MQ=255)            atgcctactACTTCACCGACATATGCATGACCGACAACCAGCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                                                                                                                                                                 <  2:94913/297‑1 (MQ=255)            atgcctactACTTCACCGACATACGCATGACCGACAACCAGCGGCACCGGGAGAGTTTTTTGCGACCACCCATCCCAGGTGTGGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGGCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                                                                                                                                                                 <  2:95923/297‑1 (MQ=255)             tgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                                                                                                                                                                 <  1:88697/297‑1 (MQ=255)                       ttCACCTACATATTCATGACCGACAACCACCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACa                                                                                                                                                                                                                                                                       <  2:122565/299‑1 (MQ=255)                              aCATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTa                                                                                                                                                                                                                                                               >  1:249786/1‑300 (MQ=255)                                atatTCATGACCGACAACCATCTGCACCGTGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGAGGTGAACGTCGGNCCCGCAGATGGCTGTGTTACGGATTTTAGTTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAATGCCCAGTTG‑T‑CTACC‑‑AATACGCGTGAATGCCTCTACTGCGCGCGTAATTTGCTCATGGGTATGCGCCGCAGACATCTGGGTAc                                                                                                                                                                                                                                                              <  1:153450/299‑1 (MQ=255)                                  atTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCATTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCTGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGaa                                                                                                                                                                                                                                                           <  2:277099/300‑1 (MQ=255)                                  atTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCATGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGaa                                                                                                                                                                                                                                                           <  2:23601/300‑1 (MQ=255)                                         accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGTTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACgcgc                                                                                                                                                                                                                                                   >  1:41751/8‑300 (MQ=255)                                         accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACgcgc                                                                                                                                                                                                                                                   >  2:488891/8‑300 (MQ=255)                                         accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACgcgc                                                                                                                                                                                                                                                   >  1:41985/8‑300 (MQ=255)                                         accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACgcgc                                                                                                                                                                                                                                                   >  1:293071/8‑300 (MQ=255)                                         accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCAAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAATGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑Acgagc                                                                                                                                                                                                                                                   >  2:88334/8‑297 (MQ=255)                                                                     ttttactaCCACTCACCCCATTTGTAGATGTGAACGTCAGTCCCGTAGATGGCTGTTTGACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGTTCATCCAGATGCCCTCTTCCGNTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCATTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCTTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATagaag                                                                                                                                                                                                                       <  2:205832/293‑1 (MQ=255)                                                                               ctgcgagatgtgtatcagagacagcGTCAGTCCCGCAGATGGGTTTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCAGCTCAGGAACGTCGGTCATCCCGATGCCCTCTTCCGCTTTCAGTTTGGATAAAGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTCCC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGt                                                                                                                                                                                                               >  1:425810/25‑298 (MQ=255)                                                                                        aGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAAt                                                                                                                                                                                                      <  1:196276/298‑1 (MQ=255)                                                                                        aGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGc                                                                                                                                                                                                    <  1:448839/300‑1 (MQ=255)                                                                                          ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCNTTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGcac                                                                                                                                                                                                  >  2:500649/4‑298 (MQ=255)                                                                                          ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCNTTTCACATCCTCA‑GGCGATAACGCACAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCAGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGACCGACCGGATAGAAGAAACCGGTGACGTAAATGcc                                                                                                                                                                                                   >  2:500780/4‑299 (MQ=255)                                                                                          ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGAACTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGcac                                                                                                                                                                                                  >  2:461279/4‑298 (MQ=255)                                                                                                   tgAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGc                                                                                                                                                                                         <  1:698290/300‑1 (MQ=255)                                                                                                   tgAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGc                                                                                                                                                                                         <  1:633193/300‑1 (MQ=255)                                                                                                                         cTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTgc                                                                                                                                                                    >  1:171958/1‑299 (MQ=255)                                                                                                                                  ggattgtaagtagcagATCTTTATGACCCAAGT‑CCGGCTCAGGAACGGCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGTGTTTATTTCACATCCTCA‑TGCGATAACGACCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAAGTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                                          <  2:88665/289‑1 (MQ=255)                                                                                                                                  ggATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GTCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                                          <  1:529025/300‑1 (MQ=255)                                                                                                                                  ggATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                                          <  1:91810/300‑1 (MQ=255)                                                                                                                                    aTTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCa                                                                                                                                                        >  1:47973/1‑300 (MQ=255)                                                                                                                                     ttttAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCAt                                                                                                                                                       <  1:102423/300‑1 (MQ=255)                                                                                                                                       ttAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAGATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCACCTACCGCATc                                                                                                                                                      >  2:682250/1‑299 (MQ=255)                                                                                                                                              gcagATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                                                                              <  2:644346/300‑1 (MQ=255)                                                                                                                                              gcagATCGGTATG‑ACCCAGGTCCGGCTCAGGAATGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTGTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAANGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATATAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                                                                              <  2:214910/300‑1 (MQ=255)                                                                                                                                                            acccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGACTGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaataat                                                                                                                                 >  2:652167/5‑300 (MQ=255)                                                                                                                                                            acccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaataat                                                                                                                                 >  2:130804/5‑300 (MQ=255)                                                                                                                                                                atttccggctcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATgg                                                                                                                               <  1:575584/288‑1 (MQ=255)                                                                                                                                                                aGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATgg                                                                                                                               <  1:61188/298‑1 (MQ=255)                                                                                                                                                                 gTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGt                                                                                                                            >  1:285930/1‑300 (MQ=255)                                                                                                                                                                         tcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGc                                                                                                                     <  2:256263/298‑1 (MQ=255)                                                                                                                                                                           aGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTNCC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGATCGGAATAATGGCGTGATCGGCTcc                                                                                                                  <  2:546466/300‑1 (MQ=255)                                                                                                                                                                               acggcGGTCATCCAGATGCCCTCTGCCGCTTGCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTGG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCGCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAACATGACCGGAATAATGGCGTGATCGGCTCCCGcc                                                                                                              <  2:385285/296‑1 (MQ=255)                                                                                                                                                                               aCGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGcc                                                                                                              <  1:626096/300‑1 (MQ=255)                                                                                                                                                                                    ggTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGt                                                                                                         <  1:383266/300‑1 (MQ=255)                                                                                                                                                                                        aTCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTAA‑GGCTATAACACCCGACCG‑T‑TTACC‑‑AATACGCGCAACTGCTTCTACTGCGCGCGTAAttcgttc                                                                                                                                                                                                                                                                                           >  1:320568/1‑114 (MQ=255)                                                                                                                                                                                           cAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGNGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGccc                                                                                                  >  1:497484/1‑300 (MQ=255)                                                                                                                                                                                           cAGATGCCCTCTTCCGCTTTCAGTTCGGATAACGCTCTCATTTCACATCCTAA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑CATACGCGTGAATGCTCCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCtt                                                                                                                                                                                                                                           >  2:637987/1‑163 (MQ=255)                                                                                                                                                                                            aGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCATTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGcc                                                                                                   <  2:106785/298‑1 (MQ=255)                                                                                                                                                                                             gATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGCTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGACCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCccgc                                                                                                >  1:340375/1‑300 (MQ=255)                                                                                                                                                                                                                    tGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACt                                                                       <  1:221111/300‑1 (MQ=255)                                                                                                                                                                                                                               tcattgtcACATCCTCANGGCGATAACGCCCAGTTGNT‑TTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                                   <  1:581977/294‑1 (MQ=255)                                                                                                                                                                                                                               tcattgtcACATCCTCANGGCGATAACGCCCAGTTG‑NCTTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                                   <  1:582003/294‑1 (MQ=255)                                                                                                                                                                                                                               tcatcgtcACATCCTCANGGCGATAACGCCCAGTTG‑NCTTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                                   <  1:581985/294‑1 (MQ=255)                                                                                                                                                                                                                                       acaTCCTCA‑TGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGGAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAg                                                    <  1:669016/300‑1 (MQ=255)                                                                                                                                                                                                                                       acaTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAg                                                    <  1:666356/300‑1 (MQ=255)                                                                                                                                                                                                                                         aTCCTCA‑GTCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTANGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGa                                                   <  1:500780/299‑1 (MQ=255)                                                                                                                                                                                                                                         aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGAc                                                  <  1:116769/300‑1 (MQ=255)                                                                                                                                                                                                                                         aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGAc                                                  <  2:139324/300‑1 (MQ=255)                                                                                                                                                                                                                                         aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTANGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGa                                                   <  1:500649/299‑1 (MQ=255)                                                                                                                                                                                                                                                  gCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGc                                          <  2:140968/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACAAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGc                                          <  2:180412/298‑1 (MQ=255)                                                                                                                                                                                                                                                          gCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTCCTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTANATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCACGCAGCCGACATTT‑‑GCTCACGGAACTGACGAGCGTTCGCCCACAGACGGTCACGCAGCTCGCt                                  >  2:515835/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCt                                  <  2:51346/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gttg‑t‑tTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ATGCGCCTGACCTTTAGGAACGCCCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATCTCTGCGCCACTACCGCATCGACAAGCACGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGATAAAGCCCGCCACCGAAATTT‑‑GCCCACGGAACTGAAGCGCGTTCGCACACAGACGGTCACGCAGCTCGCTGccc                              >  2:41720/1‑299 (MQ=255)                                                                                                                                                                                                                                                                agttacttACC‑‑AATACGNCTGAATGCTTCTACTGCGNCCGTAATGTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTNACGCAGCTCGCTGccc                              <  1:584891/294‑1 (MQ=255)                                                                                                                                                                                                                                                                agttacttACC‑‑AATACGNATGAATGCTTCTACTGCGNGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTNACGCAGCTCGCTGccc                              <  1:584915/294‑1 (MQ=255)                                                                                                                                                                                                                                                                      ttACC‑‑AATACGCGTGAATGCTTCTACTGCGGGTGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAACGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCAGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCAAGCCGCTCGCTGCCCGCTTca                        >  2:121647/1‑299 (MQ=255)                                                                                                                                                                                                                                                                             aaTACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCa                    <  1:446411/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cgcgTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTATGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGACCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTcc               >  2:460945/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         tGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTCACGCGCGTTCGCCCACAGACGGTCAAGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACttt       >  1:160035/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               tACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATgg  <  1:71802/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                               tACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATgg  <  1:71867/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                        CTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATG‑CCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGG  >  NC_000913/3791120‑3791698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑