Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,391	A→G	F387F (TTT→TTC)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,391	0	A	G	98.8%	287.6 / ‑5.8	85	F387F (TTT→TTC)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (27/57);  total (28/57)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

GATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATG‑CCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCC  >  NC_000913/3791103‑3791685                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          aaTCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCTACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  2:570375/299‑1 (MQ=255) aaTCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  1:371003/299‑1 (MQ=255) aaTCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  2:657473/299‑1 (MQ=255) aaTCTTAAAGCCTTGCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  2:597638/299‑1 (MQ=255) aaTCTTAAAGCCCTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  2:91895/299‑1 (MQ=255) aaTCTTAAAACCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGGCCCGCAGNTGGCTGTTTTAAGGATTGTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  2:217310/299‑1 (MQ=255)   tCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGATATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATCGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTg                                                                                                                                                                                                                                                                                              <  2:287799/300‑1 (MQ=255)   tCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTg                                                                                                                                                                                                                                                                                              <  1:105307/300‑1 (MQ=255)    cttaaaGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGc                                                                                                                                                                                                                                                                                             >  2:446411/5‑300 (MQ=255)     ttaaaGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATGTTAATTAGCAGATCTTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  2:159236/293‑1 (MQ=255)      taaaGCCTTTCACTTCCTGACCGATACCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACCCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTGCGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  2:93103/293‑1 (MQ=255)      gaaaGCCTTTCACTTCCTGACCGATGCCGACTACGTCACCGACATATCCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGTGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCATTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  2:92942/293‑1 (MQ=255)             tttCACTTCCTGACCGATGCCTGCTACTTCACCGACATATTCATGACCGACAACCATCGACGCCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTAc                                                                                                                                                                                                                                                                                                <  2:92805/288‑1 (MQ=255)                  cTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCt                                                                                                                                                                                                                                                                               >  2:633193/1‑300 (MQ=255)                        gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATTGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCTGCTCATGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                                                                                                                                                                                                                         <  2:462397/292‑1 (MQ=255)                        gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                                                                                                                                                                                                                         <  1:422338/292‑1 (MQ=255)                         accgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGNAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAGTTTGCTCAGGGGt                                                                                                                                                                                                                                                                        >  2:515719/8‑300 (MQ=255)                         accgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGNAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGt                                                                                                                                                                                                                                                                        >  2:515728/8‑300 (MQ=255)                             atgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                                                                                                                                                    <  1:349063/297‑1 (MQ=255)                             atgcctactACTTCACCGACATATGCATGACCGACAACCAGCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                                                                                                                                                    <  2:94913/297‑1 (MQ=255)                             atgcctactACTTCACCGACATACGCATGACCGACAACCAGCGGCACCGGGAGAGTTTTTTGCGACCACCCATCCCAGGTGTGGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGGCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                                                                                                                                                    <  2:95923/297‑1 (MQ=255)                              tgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                                                                                                                                                    <  1:88697/297‑1 (MQ=255)                                        ttCACCTACATATTCATGACCGACAACCACCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACa                                                                                                                                                                                                                                                          <  2:122565/299‑1 (MQ=255)                                               aCATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTa                                                                                                                                                                                                                                                  >  1:249786/1‑300 (MQ=255)                                                 atatTCATGACCGACAACCATCTGCACCGTGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGAGGTGAACGTCGGNCCCGCAGATGGCTGTGTTACGGATTTTAGTTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAATGCCCAGTTG‑T‑CTACC‑‑AATACGCGTGAATGCCTCTACTGCGCGCGTAATTTGCTCATGGGTATGCGCCGCAGACATCTGGGTAc                                                                                                                                                                                                                                                 <  1:153450/299‑1 (MQ=255)                                                   atTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCATTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCTGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGaa                                                                                                                                                                                                                                              <  2:277099/300‑1 (MQ=255)                                                   atTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCATGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGaa                                                                                                                                                                                                                                              <  2:23601/300‑1 (MQ=255)                                                          accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGTTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACgcgc                                                                                                                                                                                                                                      >  1:41751/8‑300 (MQ=255)                                                          accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACgcgc                                                                                                                                                                                                                                      >  1:41985/8‑300 (MQ=255)                                                          accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACgcgc                                                                                                                                                                                                                                      >  2:488891/8‑300 (MQ=255)                                                          accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACgcgc                                                                                                                                                                                                                                      >  1:293071/8‑300 (MQ=255)                                                          accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCAAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAATGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑Acgagc                                                                                                                                                                                                                                      >  2:88334/8‑297 (MQ=255)                                                                                      ttttactaCCACTCACCCCATTTGTAGATGTGAACGTCAGTCCCGTAGATGGCTGTTTGACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGTTCATCCAGATGCCCTCTTCCGNTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCATTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCTTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATagaag                                                                                                                                                                                                          <  2:205832/293‑1 (MQ=255)                                                                                                ctgcgagatgtgtatcagagacagcGTCAGTCCCGCAGATGGGTTTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCAGCTCAGGAACGTCGGTCATCCCGATGCCCTCTTCCGCTTTCAGTTTGGATAAAGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTCCC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGt                                                                                                                                                                                                  >  1:425810/25‑298 (MQ=255)                                                                                                         aGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAAt                                                                                                                                                                                         <  1:196276/298‑1 (MQ=255)                                                                                                         aGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGc                                                                                                                                                                                       <  1:448839/300‑1 (MQ=255)                                                                                                           ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCNTTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGcac                                                                                                                                                                                     >  2:500649/4‑298 (MQ=255)                                                                                                           ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCNTTTCACATCCTCA‑GGCGATAACGCACAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCAGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGACCGACCGGATAGAAGAAACCGGTGACGTAAATGcc                                                                                                                                                                                      >  2:500780/4‑299 (MQ=255)                                                                                                           ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGAACTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGcac                                                                                                                                                                                     >  2:461279/4‑298 (MQ=255)                                                                                                                    tgAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGc                                                                                                                                                                            <  1:698290/300‑1 (MQ=255)                                                                                                                    tgAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGc                                                                                                                                                                            <  1:633193/300‑1 (MQ=255)                                                                                                                                          cTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTgc                                                                                                                                                       >  1:171958/1‑299 (MQ=255)                                                                                                                                                   ggattgtaagtagcagATCTTTATGACCCAAGT‑CCGGCTCAGGAACGGCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGTGTTTATTTCACATCCTCA‑TGCGATAACGACCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAAGTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                             <  2:88665/289‑1 (MQ=255)                                                                                                                                                   ggATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GTCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                             <  1:529025/300‑1 (MQ=255)                                                                                                                                                   ggATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                             <  1:91810/300‑1 (MQ=255)                                                                                                                                                     aTTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCa                                                                                                                                           >  1:47973/1‑300 (MQ=255)                                                                                                                                                      ttttAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCAt                                                                                                                                          <  1:102423/300‑1 (MQ=255)                                                                                                                                                        ttAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAGATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCACCTACCGCATc                                                                                                                                         >  2:682250/1‑299 (MQ=255)                                                                                                                                                               gcagATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                                                                 <  2:644346/300‑1 (MQ=255)                                                                                                                                                               gcagATCGGTATG‑ACCCAGGTCCGGCTCAGGAATGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTGTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAANGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATATAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                                                                 <  2:214910/300‑1 (MQ=255)                                                                                                                                                                             acccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGACTGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaataat                                                                                                                    >  2:652167/5‑300 (MQ=255)                                                                                                                                                                             acccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaataat                                                                                                                    >  2:130804/5‑300 (MQ=255)                                                                                                                                                                                 atttccggctcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATgg                                                                                                                  <  1:575584/288‑1 (MQ=255)                                                                                                                                                                                 aGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATgg                                                                                                                  <  1:61188/298‑1 (MQ=255)                                                                                                                                                                                  gTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGt                                                                                                               >  1:285930/1‑300 (MQ=255)                                                                                                                                                                                          tcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGc                                                                                                        <  2:256263/298‑1 (MQ=255)                                                                                                                                                                                            aGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTNCC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGATCGGAATAATGGCGTGATCGGCTcc                                                                                                     <  2:546466/300‑1 (MQ=255)                                                                                                                                                                                                acggcGGTCATCCAGATGCCCTCTGCCGCTTGCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCACAGTGG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCGCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAACATGACCGGAATAATGGCGTGATCGGCTCCCGcc                                                                                                 <  2:385285/296‑1 (MQ=255)                                                                                                                                                                                                aCGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGcc                                                                                                 <  1:626096/300‑1 (MQ=255)                                                                                                                                                                                                     ggTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGt                                                                                            <  1:383266/300‑1 (MQ=255)                                                                                                                                                                                                         aTCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTAA‑GGCTATAACACCCGACCG‑T‑TTACC‑‑AATACGCGCAACTGCTTCTACTGCGCGCGTAAttcgttc                                                                                                                                                                                                                                                                              >  1:320568/1‑114 (MQ=255)                                                                                                                                                                                                            cAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGNGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGccc                                                                                     >  1:497484/1‑300 (MQ=255)                                                                                                                                                                                                            cAGATGCCCTCTTCCGCTTTCAGTTCGGATAACGCTCTCATTTCACATCCTAA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑CATACGCGTGAATGCTCCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCtt                                                                                                                                                                                                                              >  2:637987/1‑163 (MQ=255)                                                                                                                                                                                                             aGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCATTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGcc                                                                                      <  2:106785/298‑1 (MQ=255)                                                                                                                                                                                                              gATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGCTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGACCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCccgc                                                                                   >  1:340375/1‑300 (MQ=255)                                                                                                                                                                                                                                     tGGATAACGCTTTCATTTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACt                                                          <  1:221111/300‑1 (MQ=255)                                                                                                                                                                                                                                                tcattgtcACATCCTCANGGCGATAACGCCCAGTTGNT‑TTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                      <  1:581977/294‑1 (MQ=255)                                                                                                                                                                                                                                                tcattgtcACATCCTCANGGCGATAACGCCCAGTTG‑NCTTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                      <  1:582003/294‑1 (MQ=255)                                                                                                                                                                                                                                                tcatcgtcACATCCTCANGGCGATAACGCCCAGTTG‑NCTTACCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATCGCGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTCNGCTCACGGAACTGAcg                                                      <  1:581985/294‑1 (MQ=255)                                                                                                                                                                                                                                                        acaTCCTCA‑TGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGGAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAg                                       <  1:669016/300‑1 (MQ=255)                                                                                                                                                                                                                                                        acaTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAg                                       <  1:666356/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aTCCTCA‑GTCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTANGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGa                                      <  1:500780/299‑1 (MQ=255)                                                                                                                                                                                                                                                          aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGAc                                     <  1:116769/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGAc                                     <  2:139324/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aTCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTANGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGa                                      <  1:500649/299‑1 (MQ=255)                                                                                                                                                                                                                                                                   gCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGc                             <  2:140968/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     gATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACAAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGc                             <  2:180412/298‑1 (MQ=255)                                                                                                                                                                                                                                                                           gCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTCCTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTANATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCACGCAGCCGACATTT‑‑GCTCACGGAACTGACGAGCGTTCGCCCACAGACGGTCACGCAGCTCGCt                     >  2:515835/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           gCCCAGTTG‑T‑TTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCt                     <  2:51346/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gttg‑t‑tTACC‑‑AATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ATGCGCCTGACCTTTAGGAACGCCCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATCTCTGCGCCACTACCGCATCGACAAGCACGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGATAAAGCCCGCCACCGAAATTT‑‑GCCCACGGAACTGAAGCGCGTTCGCACACAGACGGTCACGCAGCTCGCTGccc                 >  2:41720/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                 agttacttACC‑‑AATACGNCTGAATGCTTCTACTGCGNCCGTAATGTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTNACGCAGCTCGCTGccc                 <  1:584891/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                 agttacttACC‑‑AATACGNATGAATGCTTCTACTGCGNGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTNACGCAGCTCGCTGccc                 <  1:584915/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ttACC‑‑AATACGCGTGAATGCTTCTACTGCGGGTGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAACGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCAGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCAAGCCGCTCGCTGCCCGCTTca           >  2:121647/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                              aaTACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCa       <  1:446411/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  cgcgTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTATGCGCCACTACCGCATCGCCAAGCATGAACGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGACCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTcc  >  2:460945/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          GATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATG‑CCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCA‑GGCGATAACGCCCAGTTG‑T‑TTACC‑‑AATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAAT‑ACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTT‑‑GCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCC  >  NC_000913/3791103‑3791685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑