Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,277	G→C	L23L (CTC→CTG)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,277	0	G	C	97.8%	317.7 / ‑5.0	92	L23L (CTC→CTG)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (43/47);  minor base A (1/1);  total (44/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATG‑CCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCA  >  NC_000913/3790988‑3791560                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                            agcCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCATAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCggct                                                                                                                                                                                                                                                                                   <  2:172470/300‑3 (MQ=255) agcCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCATAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCggct                                                                                                                                                                                                                                                                                   <  1:8701/300‑3 (MQ=255) agcCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCATAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACANATTCATGACCGACAACCATCGGCACCGGGATANTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCggct                                                                                                                                                                                                                                                                                   <  2:520070/300‑3 (MQ=255) agcCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGNGACGCGATCGCCAATCTTAAAGCCTTTCACTTNCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCAAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCggct                                                                                                                                                                                                                                                                                   >  2:529025/1‑298 (MQ=255) agcCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCggct                                                                                                                                                                                                                                                                                   >  1:180412/1‑298 (MQ=255)       gacggttAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGaa                                                                                                                                                                                                                                                                             >  1:214910/6‑300 (MQ=255)          ggttAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGt                                                                                                                                                                                                                                                                          >  1:88665/3‑300 (MQ=255)          ggttAACATCGACGCCAAGCGTGTTGCGGCACAGAGGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATTACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGt                                                                                                                                                                                                                                                                          <  2:262170/298‑1 (MQ=255)           gttAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACAGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTc                                                                                                                                                                                                                                                                         >  2:575584/2‑300 (MQ=255)           gttAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCAACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTc                                                                                                                                                                                                                                                                         >  2:598308/2‑300 (MQ=255)                cgccgaCGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTCCGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCa                                                                                                                                                                                                                                                                    >  2:666356/6‑300 (MQ=255)                cgccgaCGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCa                                                                                                                                                                                                                                                                    >  2:669016/6‑300 (MQ=255)                          gaTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGc                                                                                                                                                                                                                                                           <  1:551620/298‑1 (MQ=255)                                           gaTGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTCCGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGtt                                                                                                                                                                                                                                         >  2:584915/2‑300 (MQ=255)                                           gaTGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTACTGACCGATGCCTACTACTTCAACGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTAAGCTTTCAGtt                                                                                                                                                                                                                                         >  2:584891/2‑300 (MQ=255)                                             tGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATNTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGttt                                                                                                                                                                                                                                        >  1:140968/1‑299 (MQ=255)                                              gggTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGCCATATTCATGACCGACAACTATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCAAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGCTGCACTCTTCCGCTTTCAGttt                                                                                                                                                                                                                                        >  2:61188/1‑298 (MQ=255)                                              gggTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGttt                                                                                                                                                                                                                                        >  1:51346/1‑298 (MQ=255)                                              gggTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGATTTCAGttt                                                                                                                                                                                                                                        >  1:644346/1‑298 (MQ=255)                                                gTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGAt                                                                                                                                                                                                                                    >  1:256263/1‑300 (MQ=255)                                                     accgccacGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATNCCAGTTGTAGATGTGAACGTCAGTCCCGCAGANGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGc                                                                                                                                                                                                                               <  2:530486/296‑1 (MQ=255)                                                                       acagtGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCa                                                                                                                                                                                                             >  1:156476/5‑300 (MQ=255)                                                                                gcaGGTGATATGGCCTTCTCTAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACNCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAAc                                                                                                                                                                                                    <  2:219087/299‑1 (MQ=255)                                                                                  agttGATATGGCCTTCTCCATAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGc                                                                                                                                                                                                  <  1:155829/297‑1 (MQ=255)                                                                                  aGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGc                                                                                                                                                                                                  <  1:208322/300‑1 (MQ=255)                                                                                     tGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCa                                                                                                                                                                                               >  1:106785/1‑300 (MQ=255)                                                                                     tGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTAACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCa                                                                                                                                                                                               >  1:553491/1‑300 (MQ=255)                                                                                      gATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCa                                                                                                                                                                                               >  1:385285/1‑299 (MQ=255)                                                                                      gATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTAAGGCGATAACGCCCa                                                                                                                                                                                               >  1:546466/1‑299 (MQ=255)                                                                                       atatGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACNTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgt                                                                                                                                                                                             >  1:139324/1‑300 (MQ=255)                                                                                         atGGCCTTCTCCATAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGGTGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTTGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttg                                                                                                                                                                                           <  2:124953/300‑1 (MQ=255)                                                                                         atGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttg                                                                                                                                                                                           <  1:576200/300‑1 (MQ=255)                                                                                         atGGCCTTCCCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttg                                                                                                                                                                                           <  1:576990/300‑1 (MQ=255)                                                                                          tGGCCTTCTCCATAGACGCGATCGCCAATCTTAAAGCCCTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACANCCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttgt                                                                                                                                                                                          <  2:553491/300‑1 (MQ=255)                                                                                            gCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGttt                                                                                                                                                                                        >  2:116769/1‑300 (MQ=255)                                                                                               ttctccAGAGACACGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACCTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTAc                                                                                                                                                                                      <  2:152780/295‑1 (MQ=255)                                                                                                tctccAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATTCCCTCTTCCGCTTTCAGTTTGGATAATGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCa                                                                                                                                                                                    >  2:581977/4‑300 (MQ=255)                                                                                                tctccAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCa                                                                                                                                                                                    >  2:581985/4‑300 (MQ=255)                                                                                                tctccAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTACTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAAGGCTTTCATTTCACATCCTCAGGCGATAAAGCACAGTTGTTTACCa                                                                                                                                                                                    >  2:582003/4‑300 (MQ=255)                                                                                                tctccAGAGACGCGATCGACAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCACGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCAGCAGCTGGCTGTTTTACGGATTTTAATTAGCCGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAAGGATTTCATTTCACATGCTCAGGCGATAACGCAAAGTTGTTTAcc                                                                                                                                                                                     >  2:581970/4‑299 (MQ=255)                                                                                                tCGCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCTGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCATGCGATAACGCCCAGTTGTTTAc                                                                                                                                                                                      <  2:67494/298‑1 (MQ=255)                                                                                                 ctccAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCAAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGTGATAACGCCCCGTTGTTTACCaa                                                                                                                                                                                   >  2:383266/3‑300 (MQ=255)                                                                                                                    aaTCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCTACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  2:570375/299‑1 (MQ=255)                                                                                                                    aaTCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  1:371003/299‑1 (MQ=255)                                                                                                                    aaTCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  2:657473/299‑1 (MQ=255)                                                                                                                    aaTCTTAAAGCCTTGCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  2:597638/299‑1 (MQ=255)                                                                                                                    aaTCTTAAAGCCCTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  2:91895/299‑1 (MQ=255)                                                                                                                    aaTCTTAAAACCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGGCCCGCAGNTGGCTGTTTTAAGGATTGTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  2:217310/299‑1 (MQ=255)                                                                                                                      tCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGATATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATCGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTg                                                                                                                                                              <  2:287799/300‑1 (MQ=255)                                                                                                                      tCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTg                                                                                                                                                              <  1:105307/300‑1 (MQ=255)                                                                                                                       cttaaaGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGc                                                                                                                                                             >  2:446411/5‑300 (MQ=255)                                                                                                                        ttaaaGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATGTTAATTAGCAGATCTTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  2:159236/293‑1 (MQ=255)                                                                                                                         taaaGCCTTTCACTTCCTGACCGATACCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACCCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTGCGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  2:93103/293‑1 (MQ=255)                                                                                                                         gaaaGCCTTTCACTTCCTGACCGATGCCGACTACGTCACCGACATATCCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGTGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCATTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  2:92942/293‑1 (MQ=255)                                                                                                                                tttCACTTCCTGACCGATGCCTGCTACTTCACCGACATATTCATGACCGACAACCATCGACGCCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                                                                                <  2:92805/288‑1 (MQ=255)                                                                                                                                     cTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCt                                                                                                                                               >  2:633193/1‑300 (MQ=255)                                                                                                                                           gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATTGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCTGCTCATGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                                                                                         <  2:462397/292‑1 (MQ=255)                                                                                                                                           gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                                                                                         <  1:422338/292‑1 (MQ=255)                                                                                                                                            accgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGNAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAGTTTGCTCAGGGGt                                                                                                                                        >  2:515719/8‑300 (MQ=255)                                                                                                                                            accgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGNAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGt                                                                                                                                        >  2:515728/8‑300 (MQ=255)                                                                                                                                                atgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                    <  1:349063/297‑1 (MQ=255)                                                                                                                                                atgcctactACTTCACCGACATATGCATGACCGACAACCAGCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                    <  2:94913/297‑1 (MQ=255)                                                                                                                                                atgcctactACTTCACCGACATACGCATGACCGACAACCAGCGGCACCGGGAGAGTTTTTTGCGACCACCCATCCCAGGTGTGGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGGCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                    <  2:95923/297‑1 (MQ=255)                                                                                                                                                 tgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATgc                                                                                                                                    <  1:88697/297‑1 (MQ=255)                                                                                                                                                           ttCACCTACATATTCATGACCGACAACCACCGGCACCTGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACa                                                                                                                          <  2:122565/299‑1 (MQ=255)                                                                                                                                                                  aCATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTa                                                                                                                  >  1:249786/1‑300 (MQ=255)                                                                                                                                                                    atatTCATGACCGACAACCATCTGCACCGTGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGAGGTGAACGTCGGNCCCGCAGATGGCTGTGTTACGGATTTTAGTTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAATGCCCAGTTGTCTACCAATACGCGTGAATGCCTCTACTGCGCGCGTAATTTGCTCATGGGTATGCGCCGCAGACATCTGGGTAc                                                                                                                 <  1:153450/299‑1 (MQ=255)                                                                                                                                                                      atTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCATTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCTGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGaa                                                                                                              <  2:277099/300‑1 (MQ=255)                                                                                                                                                                      atTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCATGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGaa                                                                                                              <  2:23601/300‑1 (MQ=255)                                                                                                                                                                             accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGTTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACgcgc                                                                                                       >  1:41751/8‑300 (MQ=255)                                                                                                                                                                             accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACgcgc                                                                                                       >  2:488891/8‑300 (MQ=255)                                                                                                                                                                             accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACgcgc                                                                                                       >  1:41985/8‑300 (MQ=255)                                                                                                                                                                             accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCACAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACgcgc                                                                                                       >  1:293071/8‑300 (MQ=255)                                                                                                                                                                             accgacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCAAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAATGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATAcgagc                                                                                                       >  2:88334/8‑297 (MQ=255)                                                                                                                                                                                                         ttttactaCCACTCACCCCATTTGTAGATGTGAACGTCAGTCCCGTAGATGGCTGTTTGACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGTTCATCCAGATGCCCTCTTCCGNTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCATTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCTTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATagaag                                                                           <  2:205832/293‑1 (MQ=255)                                                                                                                                                                                                                   ctgcgagatgtgtatcagagacagcGTCAGTCCCGCAGATGGGTTTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCAGCTCAGGAACGTCGGTCATCCCGATGCCCTCTTCCGCTTTCAGTTTGGATAAAGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTCCCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGt                                                                   >  1:425810/25‑298 (MQ=255)                                                                                                                                                                                                                            aGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAAt                                                          <  1:196276/298‑1 (MQ=255)                                                                                                                                                                                                                            aGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGc                                                        <  1:448839/300‑1 (MQ=255)                                                                                                                                                                                                                              ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCNTTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGcac                                                      >  2:500649/4‑298 (MQ=255)                                                                                                                                                                                                                              ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCNTTTCACATCCTCAGGCGATAACGCACAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCAGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGACCGACCGGATAGAAGAAACCGGTGACGTAAATGcc                                                       >  2:500780/4‑299 (MQ=255)                                                                                                                                                                                                                              ttgtaGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGAACTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGcac                                                      >  2:461279/4‑298 (MQ=255)                                                                                                                                                                                                                                       tgAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGc                                             <  1:698290/300‑1 (MQ=255)                                                                                                                                                                                                                                       tgAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTACGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGc                                             <  1:633193/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cTGTTTTACGGATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTgc                        >  1:171958/1‑299 (MQ=255)                                                                                                                                                                                                                                                                      ggattgtaagtagcagATCTTTATGACCCAAGT‑CCGGCTCAGGAACGGCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGTGTTTATTTCACATCCTCATGCGATAACGACCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAAGTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg              <  2:88665/289‑1 (MQ=255)                                                                                                                                                                                                                                                                      ggATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGTCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg              <  1:529025/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      ggATTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg              <  1:91810/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aTTTTAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCa            >  1:47973/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         ttttAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCAt           <  1:102423/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           ttAATTAGCAGATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAGATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCACCTACCGCATc          >  2:682250/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                  gcagATCGTTATG‑ACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGTTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa  <  2:644346/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gcagATCGGTATG‑ACCCAGGTCCGGCTCAGGAATGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTGTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAANGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATATAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa  <  2:214910/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                            AGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATG‑CCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCA  >  NC_000913/3790988‑3791560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑