Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	318.1 / NA	98	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (42/56);  total (42/56)

ACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAA  >  NC_000913/1301698‑1302281                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                     aCCTTAAGTGAACCCTTTCCGTACCTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTNTACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGATGTATAAc                                                                                                                                                                                                                                                                                                 <  2:229618/300‑1 (MQ=255) aCCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACTGGGGCCAAAAGCCGCTATCTAGAAATTCGTCGAAAAATGGACCCAGCCTGTTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACAGGACGTATAAc                                                                                                                                                                                                                                                                                                 <  2:704043/300‑1 (MQ=255) aCCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                                 >  1:106329/1‑300 (MQ=255) aCCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                                 <  2:108561/300‑1 (MQ=255)  ccTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGACATCGACATGGCGTATAACa                                                                                                                                                                                                                                                                                                >  2:155605/1‑300 (MQ=255)  ccTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACa                                                                                                                                                                                                                                                                                                >  1:685265/1‑300 (MQ=255)    ttAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGTTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTATTGGTGAAATCGACATGACGTATAACAg                                                                                                                                                                                                                                                                                               <  2:336587/299‑1 (MQ=255)    ttAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAg                                                                                                                                                                                                                                                                                               <  1:671327/299‑1 (MQ=255)    ttAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAAGGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACTGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAg                                                                                                                                                                                                                                                                                               <  2:26145/299‑1 (MQ=255)        gTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCCACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTATTGGTGAAATCGACATGACGTATAACAGCATGc                                                                                                                                                                                                                                                                                          <  2:270243/300‑1 (MQ=255)        gTGAACCCGTTCCGTACTTCGATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGc                                                                                                                                                                                                                                                                                          <  2:270212/300‑1 (MQ=255)        gTGAACCCATTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGc                                                                                                                                                                                                                                                                                          <  2:270090/300‑1 (MQ=255)              ccGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATc                                                                                                                                                                                                                                                                                     >  1:600843/1‑299 (MQ=255)                gTTCCGTACTTCTATANATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGNGAAATCGACATGACGTATNACAGCATGCCGATCGaa                                                                                                                                                                                                                                                                                  >  1:593328/1‑300 (MQ=255)                gTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGaa                                                                                                                                                                                                                                                                                  >  1:609929/1‑300 (MQ=255)                          tCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTACCCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGa                                                                                                                                                                                                                                                                        >  2:579252/1‑300 (MQ=255)                                aaTTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGa                                                                                                                                                                                                                                                                  >  1:680736/1‑300 (MQ=255)                                    acgtGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTCGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaa‑aaa                                                                                                                                                                                                                                                             <  2:609929/297‑1 (MQ=255)                                    aCTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaa‑aaa                                                                                                                                                                                                                                                             <  2:593328/300‑1 (MQ=255)                                                   aaCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACATACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑ACAAGAGATCCCggcaga                                                                                                                                                                                                                                              >  2:309898/1‑296 (MQ=255)                                                             gTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGtt                                                                                                                                                                                                                                    <  1:376550/300‑1 (MQ=255)                                                             gTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGtt                                                                                                                                                                                                                                    <  2:400354/300‑1 (MQ=255)                                                               gCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCAACAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGa                                                                                                                                                                                                                                  >  1:381372/1‑300 (MQ=255)                                                                         gCTATCGAGAAATTCGGCGAAGAATGGACCTAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                          <  2:241283/298‑1 (MQ=255)                                                                         gCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGTTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                          <  1:519752/298‑1 (MQ=255)                                                                         gCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTNGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                          <  1:499065/298‑1 (MQ=255)                                                                         gCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGNC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCNACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                          <  2:524795/298‑1 (MQ=255)                                                                         gCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                          <  1:349937/298‑1 (MQ=255)                                                                           tATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                          <  2:50159/296‑1 (MQ=255)                                                                               gagaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                                                                                                   <  2:430754/299‑1 (MQ=255)                                                                               gagaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                                                                                                   <  1:214082/299‑1 (MQ=255)                                                                                agaAATTCGTCTAAAAATGGACCCAGCCTGTTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                                                                                                   <  2:231185/298‑1 (MQ=255)                                                                                  aaaTTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTAc                                                                                                                                                                                                               <  1:475896/300‑1 (MQ=255)                                                                                             aaaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAA‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCAGATCGAATTGTTCCAGACGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTCCTAttcagaaa                                                                                                                                                                                                     >  2:145755/1‑293 (MQ=255)                                                                                             aaaaTGGACCCAGCCCGGTAATATCGTCACCCACGGTGCCTATACATCAAAAGACTGGGTAGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTGACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAGTCGAACTGACGTATAACAGTATGCCGGTCGAATGGTTCCAGAAGCTGAA‑CAAAGAGGTCCCGGACGACGTTCACGGTGATCCATAACTGTGCACttgatattcaaaaat                                                                                                                                                                                                    >  2:146034/1‑287 (MQ=255)                                                                                               aaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTa                                                                                                                                                                                                  >  1:665933/1‑300 (MQ=255)                                                                                                         gCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCAGGACGAAGTTCAAGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGaa                                                                                                                                                                                        >  1:103562/1‑300 (MQ=255)                                                                                                            tGGTAATATCGTCACCNACGGTGCCTATACCTTAANAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGANATTAACAACCAGAAAc                                                                                                                                                                                      <  1:579252/299‑1 (MQ=255)                                                                                                            tGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAAcc                                                                                                                                                                                     >  1:679411/1‑300 (MQ=255)                                                                                                            tGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAAc                                                                                                                                                                                      <  1:11282/299‑1 (MQ=255)                                                                                                             ggTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAAcc                                                                                                                                                                                     >  1:560545/1‑299 (MQ=255)                                                                                                              gTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAACTGTGCACTTACTATTAAGAAATTCACAAACAGAAAcc                                                                                                                                                                                     >  2:699311/1‑298 (MQ=255)                                                                                                                        cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCNACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAAAAACCAGAAACCGCCATTCAACGa                                                                                                                                                                         >  2:220999/1‑300 (MQ=255)                                                                                                                         aCCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGa                                                                                                                                                                         <  2:649430/299‑1 (MQ=255)                                                                                                                         aCCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCATTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGa                                                                                                                                                                         <  2:358284/299‑1 (MQ=255)                                                                                                                         aCCAACGGTGCCTATACCGGAAAAGATGGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGGC‑‑TATTTCTTCTGAAGGGAA‑‑AGATGTC‑‑AACAGCTAAAGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAGTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGa                                                                                                                                                                         <  2:649589/299‑1 (MQ=255)                                                                                                                            aaCGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCTACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtg                                                                                                                                                                       <  2:106329/298‑1 (MQ=255)                                                                                                                                  gcttataCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtg                                                                                                                                                               <  1:342524/297‑1 (MQ=255)                                                                                                                                  gCCTCTACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCCGCCCGACCTACTGGAACCACGCGAAAACCGTTATTCACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGAAATGAAGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCCCtt                                                                                                                                                                                                                 >  2:652453/1‑250 (MQ=255)                                                                                                                                  gCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgt                                                                                                                                                                >  2:563167/1‑299 (MQ=255)                                                                                                                                  gCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgt                                                                                                                                                                >  1:336012/1‑299 (MQ=255)                                                                                                                                    cTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgt                                                                                                                                                                >  2:609411/1‑297 (MQ=255)                                                                                                                                       taCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTAc                                                                                                                                                          <  2:597166/300‑1 (MQ=255)                                                                                                                                           ttAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTAACGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCAGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCGGAGGCCGTCATTCAACGATGTTCGTGTGCGTACCGCa                                                                                                                                                      >  2:31496/1‑300 (MQ=255)                                                                                                                                            tAAAAGATTGGTTCGTAAACGAACGAATCGTTCTTGAGCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAc                                                                                                                                                     <  1:426349/300‑1 (MQ=255)                                                                                                                                            tAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTANCCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAc                                                                                                                                                     <  1:496199/300‑1 (MQ=255)                                                                                                                                            tAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAc                                                                                                                                                     <  1:427550/300‑1 (MQ=255)                                                                                                                                             aaaaaaTTGGGTCATAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGGATAACGCGAGAATTGTTAGTAACCAGGTAACCTATTTGCC‑‑TATTGCTGCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGATGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGATATCCCGGACGAAGTTCACGTTGATCCATAc                                                                                                                                                                                                                           <  2:374344/229‑1 (MQ=255)                                                                                                                                                   ttGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACt                                                                                                                                              <  1:340155/300‑1 (MQ=255)                                                                                                                                                           tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCNCTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTAt                                                                                                                                        >  1:136551/1‑298 (MQ=255)                                                                                                                                                             aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCTTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAc                                                                                                                                    <  2:381372/300‑1 (MQ=255)                                                                                                                                                             aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTTTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAc                                                                                                                                    <  2:680736/300‑1 (MQ=255)                                                                                                                                                             aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAc                                                                                                                                    <  1:563167/300‑1 (MQ=255)                                                                                                                                                               cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAcc                                                                                                                                   >  1:119570/1‑299 (MQ=255)                                                                                                                                                               cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACCGAAACTAGGTATGGAcc                                                                                                                                   >  1:120253/1‑299 (MQ=255)                                                                                                                                                                          ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCCTGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTAAGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACAGTGACATCAtt                                                                                                                        >  2:169095/1‑299 (MQ=255)                                                                                                                                                                          ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAtt                                                                                                                        >  1:224043/1‑299 (MQ=255)                                                                                                                                                                          ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCACACTGAAACTCGGTa                                                                                                                                         >  2:11457/1‑282 (MQ=255)                                                                                                                                                                               gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTNC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACTAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCAGTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaat                                                                                                                  <  1:131504/300‑1 (MQ=255)                                                                                                                                                                                          gACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTACTAAAGTGAAAgc                                                                                                       >  1:573811/1‑300 (MQ=255)                                                                                                                                                                                          gACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAgc                                                                                                       >  1:573803/1‑300 (MQ=255)                                                                                                                                                                                           aCCTACTGGAACAACGNGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                                 >  1:593857/1‑114 (MQ=255)                                                                                                                                                                                           aCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                                 >  1:247817/1‑114 (MQ=255)                                                                                                                                                                                           aCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                                 >  1:250199/1‑114 (MQ=255)                                                                                                                                                                                           aCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                                 <  2:250199/114‑1 (MQ=255)                                                                                                                                                                                           aCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                                 <  2:247817/114‑1 (MQ=255)                                                                                                                                                                                           aCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                                 <  2:593857/114‑1 (MQ=255)                                                                                                                                                                                           aCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCCCTTCCTCTTACGAAATTCACCACCAGAAACCGCCATTCAACGCTGTGCGTGTGCGTACAGCACTGAAACTCGGTATGGACCGCGATATCATTGTTAATAAAGt                                                                                                             >  2:69869/1‑293 (MQ=255)                                                                                                                                                                                              tACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACTGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCa                                                                                                    >  1:387558/1‑299 (MQ=255)                                                                                                                                                                                              tACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCa                                                                                                    >  1:386728/1‑299 (MQ=255)                                                                                                                                                                                                  ggAACAAGGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCATTGAAACTAGGTATGGAc                                                                                                                                    <  1:233541/263‑1 (MQ=255)                                                                                                                                                                                                             aaaaCCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGcc                                                                                  <  1:696940/300‑1 (MQ=255)                                                                                                                                                                                                                         aaCCAGGTAACCTACGTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATTTC‑‑AACCGCTACCGTAGTGGGGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCTCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTacac                                                                       <  2:59147/299‑1 (MQ=255)                                                                                                                                                                                                                             gtaccgccTATTTGCCTNTATTGCTTCTGAAGTTACNGCGATGCCCCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAGAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACCNCTGCCCGCCTATGGtt                                                                           <  1:582733/294‑1 (MQ=255)                                                                                                                                                                                                                             aGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAAACAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCAc                                                                  >  1:379777/1‑300 (MQ=255)                                                                                                                                                                                                                                tAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGt                                                               <  1:102891/300‑1 (MQ=255)                                                                                                                                                                                                                                  aCCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGtat                                                             >  1:689325/1‑300 (MQ=255)                                                                                                                                                                                                                                     tATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATAc                                                           <  2:446513/299‑1 (MQ=255)                                                                                                                                                                                                                                     tATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATAc                                                           <  1:322279/299‑1 (MQ=255)                                                                                                                                                                                                                                       tttGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGGTGTC‑‑AACCGTTACCGTAGTGGTGAAATCGACAGGACGTATAACAGCATGCTGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACGATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACGCCACCGTATACTGa                                                        <  2:600843/300‑1 (MQ=255)                                                                                                                                                                                                                                        ttGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTATTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATATGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATACTGAt                                                       <  2:11941/300‑1 (MQ=255)                                                                                                                                                                                                                                                    cTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAAt                                             >  1:668426/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACt                                        <  2:560545/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    gATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGAGCAGGGCAAC‑A‑TGCACGCCTATGGTTAAACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGaa                               >  2:130934/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     aTGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATTCCGATCGAATTGTTCCAGAAGCTGAA‑AANAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAAt                              <  1:145755/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             aCCGCTACCGTGGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AANAGAGATCCCGGACGAAATTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATGAACAACCAGAAACCGCCATTCAGCGATATGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAGAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAAt                              <  1:146034/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                gCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCt                     <  1:609411/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   cGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTaa  >  1:644991/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                     ACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCC‑‑TATTGCTTCTGAAGTTAC‑‑CGATGTC‑‑AACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAA‑AAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAC‑A‑TGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAA  >  NC_000913/1301698‑1302281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑