Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,755	C→T	M109I (ATG→ATA)	waaH ←	UDP‑glucuronate:LPS(HepIII) glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,755	0	C	T	100.0%	217.2 / NA	62	M109I (ATG→ATA)	waaH	UDP‑glucuronate:LPS(HepIII) glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/29);  total (33/29)

TATCGCGCACGCAGCGCGTTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCACGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACGCCGGTTGAGCGAAGGCGATCGGTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCCATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAA  >  NC_000913/3789462‑3790050                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                        tatCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGtc                                                                                                                                                                                                                                                                                                   <  2:360201/300‑1 (MQ=255)  atCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGtca                                                                                                                                                                                                                                                                                                  <  1:608452/300‑1 (MQ=255)     gcgcACGCAGCGCATTAAACATGGATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGtcatc                                                                                                                                                                                                                                                                                                <  1:400180/299‑1 (MQ=255)              gcgcATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTAGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGt                                                                                                                                                                                                                                                                                      >  2:146201/1‑300 (MQ=255)                gcattAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAGTCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGGAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTAc                                                                                                                                                                                                                                                                                    >  2:64469/4‑300 (MQ=255)                               tCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGAc                                                                                                                                                                                                                                                                      <  2:478225/299‑1 (MQ=255)                                                         atgTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTAGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGAc                                                                                                                                                                                                                                           >  2:483918/1‑300 (MQ=255)                                                         atgTAATCCGGCAACAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGCCAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCTGCAGCAAGTCCGGGCAGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCAAGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGGTAGCCTTCATCGTCAGCATCGCCAAAAGCGAc                                                                                                                                                                                                                                           >  2:119914/1‑300 (MQ=255)                                                                          aTTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTAACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCa                                                                                                                                                                                                                          >  2:255999/1‑300 (MQ=255)                                                                                   tatgtttAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGATTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAATGCGATCGCTGGGGATGGATTGCCAGGCTTCTCCCGTTTCACGAAAACACCAGTCAGCGGTGCACTGCGCCACTTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATcc                                                                                                                                                                                                                 <  2:387176/296‑1 (MQ=255)                                                                                   tATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATcc                                                                                                                                                                                                                 <  1:701292/300‑1 (MQ=255)                                                                                   tATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATcc                                                                                                                                                                                                                 <  2:408111/300‑1 (MQ=255)                                                                                            cAATAACACCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAACCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCCCGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTAc                                                                                                                                                                                                        >  2:489880/1‑300 (MQ=255)                                                                                                           gATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATgcac                                                                                                                                                                                         >  2:697127/1‑298 (MQ=255)                                                                                                                 cccccATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCAt                                                                                                                                                                                   >  2:687194/1‑300 (MQ=255)                                                                                                                 cccccATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCAt                                                                                                                                                                                   >  1:281528/1‑300 (MQ=255)                                                                                                                     cATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGc                                                                                                                                                                               >  1:1269/1‑300 (MQ=255)                                                                                                                                   gAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAaac                                                                                                                                                                 >  1:386426/1‑300 (MQ=255)                                                                                                                                    aGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCATTCCTGGCCGGTTAATACACCGGTTGAGCGAAGTCGATCGCTGTGGATGGATTGCCATGTTTCTCCCGTGTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATAGTCATCAGCGTTTCGTACATGTTGGGATAGACTTCATCGTCAGCATCGACAAAATTGACATATTTGCCCGTGGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAaacg                                                                                                                                                                <  2:386812/300‑1 (MQ=255)                                                                                                                                     gTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAaacga                                                                                                                                                               >  1:20678/1‑300 (MQ=255)                                                                                                                                     gTCCAACGGCGCGAAGAAAGCCCCACCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCTACAAAAGCNACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAaacga                                                                                                                                                               >  1:133453/1‑300 (MQ=255)                                                                                                                                       ccAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTAAGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAaacgaa                                                                                                                                                              >  2:270367/1‑299 (MQ=255)                                                                                                                                                    gaaAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGACACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGttt                                                                                                                                                <  1:441808/300‑1 (MQ=255)                                                                                                                                                                 gcaaccAGTCCGGGCCGGTTAATACGCCGGTTGAGCGAAGGCAATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAAtgc                                                                                                                                    <  2:363598/295‑3 (MQ=255)                                                                                                                                                                 gCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAAtgc                                                                                                                                    <  2:672624/299‑3 (MQ=255)                                                                                                                                                                       aGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTa                                                                                                                             >  2:68736/1‑300 (MQ=255)                                                                                                                                                                       aGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTAAAGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTAGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTAAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCATGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTa                                                                                                                             >  2:54453/1‑300 (MQ=255)                                                                                                                                                                            gggCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTTGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGGTAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTAttt                                                                                                                          <  1:293480/298‑1 (MQ=255)                                                                                                                                                                                 ggTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCGGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGa                                                                                                                   >  2:655314/1‑300 (MQ=255)                                                                                                                                                                                 ggTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGa                                                                                                                   >  1:36220/1‑300 (MQ=255)                                                                                                                                                                                 ggTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCAATTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAGTGCTTTGCTATTTCAACa                                                                                                                     >  2:331752/1‑298 (MQ=255)                                                                                                                                                                                   ttAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGNGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAAt                                                                                                                 <  1:493416/300‑1 (MQ=255)                                                                                                                                                                                                 gAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCa                                                                                                   <  2:451559/300‑1 (MQ=255)                                                                                                                                                                                                           gATCGCTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGTTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTtaataa                                                                                         <  2:650149/300‑1 (MQ=255)                                                                                                                                                                                                                             gCCAGGTTTCTCCCGTTTCACGAGAACACCAGTCAGCGTTGCACTGCTCCACGTCGAGTTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGc                                                                         <  2:195111/298‑1 (MQ=255)                                                                                                                                                                                                                                     tctcCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCTAGGTCGTCCTCTAACGCTATAGTCATCAGCGTTTTGTACATGGTGTGATAGACTTCATCGTCAGCATCGACAAAAGTGACATATTTGCCCGTTGCCACTTCAATCCCACAATTACGCGCCACCGATGCGCCGGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGGTTTCTGCTTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGtt                                                               <  2:376075/300‑1 (MQ=255)                                                                                                                                                                                                                                     tctcCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGTTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGAGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGtt                                                               <  1:618782/300‑1 (MQ=255)                                                                                                                                                                                                                                     tctcCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGtt                                                               <  2:125868/300‑1 (MQ=255)                                                                                                                                                                                                                                     tctcCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGtt                                                               <  1:331752/300‑1 (MQ=255)                                                                                                                                                                                                                                     tctcCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTNGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGtt                                                               <  2:207663/300‑1 (MQ=255)                                                                                                                                                                                                                                                    aaaCACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGAt                                                <  1:687194/300‑1 (MQ=255)                                                                                                                                                                                                                                                               aGCGTGGCACTGCGCCACTTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAAGCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAg                                      <  2:278284/299‑1 (MQ=255)                                                                                                                                                                                                                                                                gCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGATATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTg                                    <  1:556694/300‑1 (MQ=255)                                                                                                                                                                                                                                                                gCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTTGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGt                                     >  2:394961/1‑299 (MQ=255)                                                                                                                                                                                                                                                                gCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCTCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGt                                     >  1:536021/1‑299 (MQ=255)                                                                                                                                                                                                                                                                gCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGt                                     >  1:521808/1‑299 (MQ=255)                                                                                                                                                                                                                                                                gCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGCTTGCGTAATTAAAGATTCCATACaa                                       >  2:686335/1‑297 (MQ=255)                                                                                                                                                                                                                                                                   ttGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCATGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGTAGTCCAGGTTTGCGTAATTAACGATTCCATACAAGTGc                                   >  2:267725/1‑298 (MQ=255)                                                                                                                                                                                                                                                                     gCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGaa                               >  1:643955/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gcgcCACTTCGAGGTCGTCCTCTAACGCTATATTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATTGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCTGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAatc                            <  2:279565/298‑1 (MQ=255)                                                                                                                                                                                                                                                                          gcgcCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAatcat                          >  1:104806/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        tcgtcCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATtatat            <  1:655314/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        tcgtcCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGGGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAGTTATCCGTTGAACCATTGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATtatat            <  2:609182/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ccTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTNATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGTAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAAc         >  2:494545/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                             ctctAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATaa          >  2:661197/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                             ctctAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAAc         >  2:396558/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                             ctctAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCACGCATTATATAAc         >  2:93396/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                             ctctAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGACCGCATTATATAAc         >  1:550931/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                             ctctAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCCCCCGCATTATATAAc         >  1:179841/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                              tctAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTNCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACgg       <  1:146201/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tctAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACgg       <  1:650865/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  aCGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCCTTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTTAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGACTCCATACAAGTGCGGAAATTATCGCCCGCATTATATAACGGaataa  >  2:29127/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                        TATCGCGCACGCAGCGCGTTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCACGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACGCCGGTTGAGCGAAGGCGATCGGTGGGGATGGATTGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCCATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAA  >  NC_000913/3789462‑3790050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑