Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,773	A→G	E312E (GAA→GAG)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,773	0	A	G	100.0%	194.8 / NA	55	E312E (GAA→GAG)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/27);  total (28/27)

GAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCG  >  NC_000913/3787483‑3788067                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                       gaatCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAg                                                                                                                                                                                                                                                                                               <  1:673906/297‑1 (MQ=255)                   ggTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCAACTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCAGGTTCGCGGGCCGAAGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGaaa                                                                                                                                                                                                                                                                             >  2:599382/1‑300 (MQ=255)                             agctaagtgaacTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGtg                                                                                                                                                                                                                                                                    <  1:485984/288‑2 (MQ=255)                                                   cGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAATGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGAACGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCGGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGa                                                                                                                                                                                                                                             >  2:26707/1‑300 (MQ=255)                                                     aaTCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGaa                                                                                                                                                                                                                                            >  2:438810/1‑299 (MQ=255)                                                      aTCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGt                                                                                                                                                                                                                                          >  1:527109/1‑300 (MQ=255)                                                      aTCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCAACTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGt                                                                                                                                                                                                                                          >  1:527167/1‑300 (MQ=255)                                                                cGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCTACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGAtt                                                                                                                                                                                                                                >  1:3322/1‑300 (MQ=255)                                                                             cccGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAAACGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGCTAAAGCGATTGCCGACGGt                                                                                                                                                                                                                       >  2:175153/1‑296 (MQ=255)                                                                                       aGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGTGCGAATGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCGACAGGGTGAATTACGCTGGAAATGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAATCGATTGCCGACGGTCGGGTGATTCTGGc                                                                                                                                                                                                         <  1:71951/300‑1 (MQ=255)                                                                                        gCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACTCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCt                                                                                                                                                                                                        <  2:639318/300‑1 (MQ=255)                                                                                        gCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCGGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACTCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAATCGATTGCCGACGGTCGGGTGATTCTGGCt                                                                                                                                                                                                        <  2:642569/300‑1 (MQ=255)                                                                                            cggcgAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCNGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTATGAAGGTACTGAAGTTAACGCGATTGCCGACGGTCGGGTGATTCTGGCTGACt                                                                                                                                                                                                    >  2:512469/1‑300 (MQ=255)                                                                                                        gTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGc                                                                                                                                                                                         <  1:26707/299‑1 (MQ=255)                                                                                                             gAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGt                                                                                                                                                                                   >  1:441769/1‑300 (MQ=255)                                                                                                                    aaGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtgg                                                                                                                                                                            <  1:80928/300‑1 (MQ=255)                                                                                                                         cGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAACTCCAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGCAGTTAAAGCGATTGCAGACGGTCGGGTGGTTCTGGCTGACTGGCTGCACGGCTACGGTCtggtggtggt                                                                                                                                                                        >  2:195032/1‑299 (MQ=255)                                                                                                                              ggCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTTAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGa                                                                                                                                                                  >  1:34203/1‑300 (MQ=255)                                                                                                                               gCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAATGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAg                                                                                                                                                                 <  1:560314/300‑1 (MQ=255)                                                                                                                               gCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAg                                                                                                                                                                 <  1:560650/300‑1 (MQ=255)                                                                                                                                          tCGCGACCGCCAGAAATAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAACCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGTGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAATGGATGGTGATTGGTGCCTCTGAAGGTACTTAAGTTAAAGCGATTGCCTACGTTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAgg                                                                                                                                                      <  2:27634/300‑1 (MQ=255)                                                                                                                                          tCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAgg                                                                                                                                                      <  1:89374/300‑1 (MQ=255)                                                                                                                                            gctatctacAGAAAGAAGCGACGTGCAAAGGCACCACCTACAAACCTACCGAAAGCTAAAAATTGCTGGTGTCCCGAGCTGGTTGCATGGGTGCGCCGCGTGGGCATGAATTCTGGCCGGTTCGCGTGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGTTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGGACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGTTGGTGGTTGAGCATGGTAAAgg                                                                                                                                                      <  2:32495/290‑1 (MQ=255)                                                                                                                                            gcgACCGCCAGAAGGAAGCGACGCGCAAAGGCACCACCTACAAGCCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAgg                                                                                                                                                      <  2:289101/298‑1 (MQ=255)                                                                                                                                            gcgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAATTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGTTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCAATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAgg                                                                                                                                                      <  2:289489/298‑1 (MQ=255)                                                                                                                                                 cGCCAGAAAGAAGCTACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACAt                                                                                                                                                >  1:67541/1‑299 (MQ=255)                                                                                                                                                 cGCCAGAAAGAAGCTACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACAt                                                                                                                                                >  1:67558/1‑299 (MQ=255)                                                                                                                                                                    gcAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGNTTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGNTTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                                                                            <  2:527109/300‑1 (MQ=255)                                                                                                                                                                    gcAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGNTTGGTGCCTCTGAAGGTACTGAAGGTAAAGCGNTTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                                                                            <  2:527167/300‑1 (MQ=255)                                                                                                                                                                          gCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACNGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAgc                                                                                                                       >  1:140749/1‑299 (MQ=255)                                                                                                                                                                                ccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACt                                                                                                                  >  1:570438/1‑298 (MQ=255)                                                                                                                                                                                ccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACt                                                                                                                  >  1:32764/1‑298 (MQ=255)                                                                                                                                                                                                                  gTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCATTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGcc                                                                              >  2:161622/1‑300 (MQ=255)                                                                                                                                                                                                                       gaactggtggcCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTCAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAAtt                                                                          >  2:695995/6‑299 (MQ=255)                                                                                                                                                                                                                           tggtggcCTGGGTGCGCCGCGTGGTCATGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCAc                                                                      <  1:175796/298‑1 (MQ=255)                                                                                                                                                                                                                           tggtggcCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCAc                                                                      <  1:175713/298‑1 (MQ=255)                                                                                                                                                                                                                               ggccTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACtggt                                                                  >  2:580173/4‑299 (MQ=255)                                                                                                                                                                                                                                  cTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGGTTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgc                                                              >  2:616551/1‑300 (MQ=255)                                                                                                                                                                                                                                   tGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgc                                                              <  1:645700/299‑1 (MQ=255)                                                                                                                                                                                                                                    gggtgcgcCGCGTGTTCATGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgcag                                                            <  1:630738/296‑1 (MQ=255)                                                                                                                                                                                                                                    gggtgcgcCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGANTTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgcag                                                            <  1:128423/296‑1 (MQ=255)                                                                                                                                                                                                                                           ccgcGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGc                                                     >  1:85620/1‑300 (MQ=255)                                                                                                                                                                                                                                             gcgTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCg                                                    <  1:92196/299‑1 (MQ=255)                                                                                                                                                                                                                                                     aGGCATTCTTGCCGGTTCGCGGGCCGACACTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTGCGCTGGAAAGAGCTGGTGATTTGTGCCTCTGAGGGTGCTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTAGTTGAGCATGGTAAAGTCGACGTGAGTCTTTACGGCTATAATCAGAGCGCACTGTTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTc                                           <  2:172023/300‑1 (MQ=255)                                                                                                                                                                                                                                                     aGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGTTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGNCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGt                                            <  2:497728/299‑1 (MQ=255)                                                                                                                                                                                                                                                     aGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTc                                           <  1:282666/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCAct                                 <  1:447914/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       tCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCa            >  1:427644/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                       tCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTAGAAATTcgccgaca            >  2:184835/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                         gatATGGCGCGCAGCTACAGGGTGAATTAAGCTGGAAAGGGATGGTGATTGGTGCCTATGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGATGACTGGCTGCAAGGCTCCGATCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGATTCTCTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCAAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTAACTCTATCTCGAAATTCGCCGCCAGGGTc       >  2:488767/3‑300 (MQ=255)                                                                                                                                                                                                                                                                                         gCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCCAGGCTACGGTCTGGCGGTGGTGGTTGAGCATGGTAAAGGCGCCATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGCGGGCAGCAGTGGCGGTCAGGGTCAGCATTCACTCTATTTCGAAATTcgcc                >  2:371669/1‑291 (MQ=255)                                                                                                                                                                                                                                                                                         gCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTc       >  1:265167/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         gCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTATGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTAAGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTAACTCTATTTCGAACTTCGCCGCCAGGGTc       >  2:371666/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         gCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCATCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTc       >  2:454168/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              ggCGAGCAGCTACAGGGTGAATTCCGCTGGAAATGGATGGTGATCTGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGTTGATTCTGGCTTACTGGCTGCAAGGCGACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGTGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGTTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGGAATTCGCCGCCAGGGTCGGGCg  <  2:386000/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                       GAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCG  >  NC_000913/3787483‑3788067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑