Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,713	C→T	G292G (GGC→GGT)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,713	0	C	T	100.0%	163.1 / NA	51	G292G (GGC→GGT)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/28);  total (23/28)

ACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGC  >  NC_000913/3787437‑3788007                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       aCTGACTCATGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAGCTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTg                                                                                                                                                                                                                                                                                 <  1:277490/300‑1 (MQ=255)          ggctcTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTc                                                                                                                                                                                                                                                                         >  2:447914/5‑299 (MQ=255)            ctcTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTcgc                                                                                                                                                                                                                                                                       >  1:274825/3‑299 (MQ=255)                     aGCGTAAAAAGACGCTGGCTGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGc                                                                                                                                                                                                                                                             <  2:591709/300‑1 (MQ=255)                     aGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGc                                                                                                                                                                                                                                                             <  1:38695/300‑1 (MQ=255)                      gCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCt                                                                                                                                                                                                                                                            >  1:433375/1‑300 (MQ=255)                             aaGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGc                                                                                                                                                                                                                                                     <  2:394049/300‑1 (MQ=255)                             aaGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGc                                                                                                                                                                                                                                                     <  1:471420/300‑1 (MQ=255)                                    tGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCTAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCg                                                                                                                                                                                                                                              <  2:396310/300‑1 (MQ=255)                                    tGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATgg                                                                                                                                                                                                                                                <  2:419767/298‑1 (MQ=255)                                    tGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCg                                                                                                                                                                                                                                              <  2:194434/300‑1 (MQ=255)                                    tGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCg                                                                                                                                                                                                                                              <  1:530569/300‑1 (MQ=255)                                               gaatCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAg                                                                                                                                                                                                                                   <  1:673906/297‑1 (MQ=255)                                                                 ggTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCAACTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCAGGTTCGCGGGCCGAAGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGaaa                                                                                                                                                                                                                 >  2:599382/1‑300 (MQ=255)                                                                           agctaagtgaacTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGtg                                                                                                                                                                                                        <  1:485984/288‑2 (MQ=255)                                                                                                 cGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAATGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGAACGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCGGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGa                                                                                                                                                                                 >  2:26707/1‑300 (MQ=255)                                                                                                   aaTCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGaa                                                                                                                                                                                >  2:438810/1‑299 (MQ=255)                                                                                                    aTCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGt                                                                                                                                                                              >  1:527109/1‑300 (MQ=255)                                                                                                    aTCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCAACTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGt                                                                                                                                                                              >  1:527167/1‑300 (MQ=255)                                                                                                              cGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCTACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGAtt                                                                                                                                                                    >  1:3322/1‑300 (MQ=255)                                                                                                                           cccGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAAACGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGCTAAAGCGATTGCCGACGGt                                                                                                                                                           >  2:175153/1‑296 (MQ=255)                                                                                                                                     aGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGTGCGAATGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCGACAGGGTGAATTACGCTGGAAATGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAATCGATTGCCGACGGTCGGGTGATTCTGGc                                                                                                                                             <  1:71951/300‑1 (MQ=255)                                                                                                                                      gCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACTCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCt                                                                                                                                            <  2:639318/300‑1 (MQ=255)                                                                                                                                      gCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCGGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACTCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAATCGATTGCCGACGGTCGGGTGATTCTGGCt                                                                                                                                            <  2:642569/300‑1 (MQ=255)                                                                                                                                          cggcgAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCNGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTATGAAGGTACTGAAGTTAACGCGATTGCCGACGGTCGGGTGATTCTGGCTGACt                                                                                                                                        >  2:512469/1‑300 (MQ=255)                                                                                                                                                      gTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGc                                                                                                                             <  1:26707/299‑1 (MQ=255)                                                                                                                                                           gAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGt                                                                                                                       >  1:441769/1‑300 (MQ=255)                                                                                                                                                                  aaGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtgg                                                                                                                <  1:80928/300‑1 (MQ=255)                                                                                                                                                                       cGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAACTCCAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGCAGTTAAAGCGATTGCAGACGGTCGGGTGGTTCTGGCTGACTGGCTGCACGGCTACGGTCtggtggtggt                                                                                                            >  2:195032/1‑299 (MQ=255)                                                                                                                                                                            ggCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTTAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGa                                                                                                      >  1:34203/1‑300 (MQ=255)                                                                                                                                                                             gCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAATGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAg                                                                                                     <  1:560314/300‑1 (MQ=255)                                                                                                                                                                             gCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAg                                                                                                     <  1:560650/300‑1 (MQ=255)                                                                                                                                                                                        tCGCGACCGCCAGAAATAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAACCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGTGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAATGGATGGTGATTGGTGCCTCTGAAGGTACTTAAGTTAAAGCGATTGCCTACGTTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAgg                                                                                          <  2:27634/300‑1 (MQ=255)                                                                                                                                                                                        tCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAgg                                                                                          <  1:89374/300‑1 (MQ=255)                                                                                                                                                                                          gctatctacAGAAAGAAGCGACGTGCAAAGGCACCACCTACAAACCTACCGAAAGCTAAAAATTGCTGGTGTCCCGAGCTGGTTGCATGGGTGCGCCGCGTGGGCATGAATTCTGGCCGGTTCGCGTGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGTTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGGACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGTTGGTGGTTGAGCATGGTAAAgg                                                                                          <  2:32495/290‑1 (MQ=255)                                                                                                                                                                                          gcgACCGCCAGAAGGAAGCGACGCGCAAAGGCACCACCTACAAGCCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAgg                                                                                          <  2:289101/298‑1 (MQ=255)                                                                                                                                                                                          gcgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAATTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGTTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCAATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAgg                                                                                          <  2:289489/298‑1 (MQ=255)                                                                                                                                                                                               cGCCAGAAAGAAGCTACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACAt                                                                                    >  1:67541/1‑299 (MQ=255)                                                                                                                                                                                               cGCCAGAAAGAAGCTACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACAt                                                                                    >  1:67558/1‑299 (MQ=255)                                                                                                                                                                                                                  gcAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGNTTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGNTTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                <  2:527109/300‑1 (MQ=255)                                                                                                                                                                                                                  gcAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGNTTGGTGCCTCTGAAGGTACTGAAGGTAAAGCGNTTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                <  2:527167/300‑1 (MQ=255)                                                                                                                                                                                                                        gCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACNGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAgc                                                           >  1:140749/1‑299 (MQ=255)                                                                                                                                                                                                                              ccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACt                                                      >  1:570438/1‑298 (MQ=255)                                                                                                                                                                                                                              ccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACt                                                      >  1:32764/1‑298 (MQ=255)                                                                                                                                                                                                                                                                gTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCATTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGcc                  >  2:161622/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gaactggtggcCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTCAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAAtt              >  2:695995/6‑299 (MQ=255)                                                                                                                                                                                                                                                                         tggtggcCTGGGTGCGCCGCGTGGTCATGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCAc          <  1:175796/298‑1 (MQ=255)                                                                                                                                                                                                                                                                         tggtggcCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCAc          <  1:175713/298‑1 (MQ=255)                                                                                                                                                                                                                                                                             ggccTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACtggt      >  2:580173/4‑299 (MQ=255)                                                                                                                                                                                                                                                                                cTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGGTTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgc  >  2:616551/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 tGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgc  <  1:645700/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       ACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGC  >  NC_000913/3787437‑3788007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑