Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,518	C→T	S227S (AGC→AGT)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,518	0	C	T	100.0%	218.6 / NA	61	S227S (AGC→AGT)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/33);  total (28/33)

AGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAA  >  NC_000913/3787238‑3787800                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                           aGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGTCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAAt                                                                                                                                                                                                                                                                         <  2:151518/300‑1 (MQ=255)    tGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATcc                                                                                                                                                                                                                                                                       <  1:659515/299‑1 (MQ=255)    tGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGACCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATcc                                                                                                                                                                                                                                                                       <  1:59898/299‑1 (MQ=255)       ttCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTc                                                                                                                                                                                                                                                                   <  1:188817/300‑1 (MQ=255)                       aaGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGACCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTTTGCGTAACAGCATTGc                                                                                                                                                                                                                                                   <  2:570176/300‑1 (MQ=255)                         gCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGccc                                                                                                                                                                                                                                                 <  1:379406/300‑1 (MQ=255)                                 ggACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCAAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGaa                                                                                                                                                                                                                                         >  2:560650/1‑300 (MQ=255)                                 ggACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCAATCCAGCAAGGTCAGCAACAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCAGTGCGGaa                                                                                                                                                                                                                                         >  2:560314/1‑300 (MQ=255)                                      gCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGCAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCAATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGa                                                                                                                                                                                                                                          >  2:89374/1‑294 (MQ=255)                                      gCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCcgc                                                                                                                                                                                                                                    >  1:386000/1‑300 (MQ=255)                                       cGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGACCGTGCGGAAGc                                                                                                                                                                                                                                       <  1:31866/296‑1 (MQ=255)                                         tttACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCgcggc                                                                                                                                                                                                                                 >  1:639318/1‑300 (MQ=255)                                         tttACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCACGTGCGGAAGCCgcggc                                                                                                                                                                                                                                 >  1:642569/1‑300 (MQ=255)                                              aGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCAGGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTACCCGTGCGGAAGCCGCGGCGAAAg                                                                                                                                                                                                                            <  2:147565/300‑1 (MQ=255)                                                     tttCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGANGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGc                                                                                                                                                                                                                     <  2:551027/300‑1 (MQ=255)                                                             aTCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTNAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACga                                                                                                                                                                                                              >  1:497728/1‑299 (MQ=255)                                                                   aCCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGTAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCATGCTCTGAATGAGCGTAAAAAGACGCTGTCTGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGATAAGCAc                                                                                                                                                                                                       <  2:572427/300‑1 (MQ=255)                                                                            gACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAATAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGcc                                                                                                                                                                                               <  2:365134/299‑1 (MQ=255)                                                                            gACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGcc                                                                                                                                                                                               <  2:123300/299‑1 (MQ=255)                                                                            gACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGcc                                                                                                                                                                                               <  1:484535/299‑1 (MQ=255)                                                                               aaGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAgg                                                                                                                                                                                           <  1:217977/300‑1 (MQ=255)                                                                                aGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGANCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGc                                                                                                                                                                                          <  1:131306/300‑1 (MQ=255)                                                                                aGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGc                                                                                                                                                                                          <  1:254314/300‑1 (MQ=255)                                                                                          gCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGAc                                                                                                                                                                                <  1:664304/300‑1 (MQ=255)                                                                                               gTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCa                                                                                                                                                                           >  2:485984/1‑300 (MQ=255)                                                                                                   aaGCAAACGCGTGGAGAAGTCGCCATGCAGCGTGCTGAACTGGAATAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGTGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCagaa                                                                                                                                                                        <  2:141009/299‑1 (MQ=255)                                                                                                            cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGAc                                                                                                                                                               >  2:471420/1‑299 (MQ=255)                                                                                                             gTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGAcg                                                                                                                                                              <  1:439879/299‑1 (MQ=255)                                                                                                                        gCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGTGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGACAGAAAGAAGCGACGCGCAAAGGtacc                                                                                                                                                  >  2:313898/1‑300 (MQ=255)                                                                                                                        gCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGcacc                                                                                                                                                  >  2:313269/1‑300 (MQ=255)                                                                                                                        gCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCACAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGcacc                                                                                                                                                  >  2:313169/1‑300 (MQ=255)                                                                                                                                  gTGCTGAGTTGGAAGAGAAACAAAGCGAGCAACAAATGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCATGTGAGGCCCAGGCGGTTCGCGATCGCCAGAAAGAAGCGAAGCGCAAAGGc                                                                                                                                                     >  2:92196/1‑287 (MQ=255)                                                                                                                                  gTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAac                                                                                                                                        <  1:66270/300‑1 (MQ=255)                                                                                                                                  gTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAac                                                                                                                                        <  2:483442/300‑1 (MQ=255)                                                                                                                                  gTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCAACTACAAac                                                                                                                                        >  2:630738/1‑300 (MQ=255)                                                                                                                                  gTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCAAGAAAGAAGCGACGCGCAAAGGCACCACCTACaaa                                                                                                                                         >  2:673906/1‑299 (MQ=255)                                                                                                                                  gTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCAAACGAATCCAGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACaaat                                                                                                                                        >  2:282666/1‑299 (MQ=255)                                                                                                                                       gAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGTATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAaccgacc                                                                                                                                   >  1:172023/1‑300 (MQ=255)                                                                                                                                       gAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAaccgacc                                                                                                                                   >  1:394049/1‑300 (MQ=255)                                                                                                                                        aaCTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAaccgacc                                                                                                                                   >  2:645700/1‑299 (MQ=255)                                                                                                                                           gcgagatgtgtataagagacagacaaACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGACAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGaaa                                                                                                                               >  1:40139/23‑300 (MQ=255)                                                                                                                                                                       gCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTACAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCAGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGACAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAGTCGCTGATGTctagaaat                                                                                                     >  2:175796/1‑291 (MQ=255)                                                                                                                                                                       gCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGACCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGAACGAAAGCGAAAAATCGATGATGTCCCgaact                                                                                                     >  2:175713/1‑294 (MQ=255)                                                                                                                                                                          gTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCgaactggt                                                                                                  >  1:424589/1‑291 (MQ=255)                                                                                                                                                                            tataTGAACAGCGCGCCCAACAGGCGAAACTGACTCATGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACtggtggcc                                                                                              <  2:446904/300‑3 (MQ=255)                                                                                                                                                                            tataTGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAAATGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAAAGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGAAGCGCAAAGGCACCACCTAAAAACCGACCGAAAGCGAAAAACTGCTGATGTccagaactggtggc                                                                                               >  2:128423/1‑287 (MQ=255)                                                                                                                                                                             atatGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACtggtggcc                                                                                              <  2:432565/299‑3 (MQ=255)                                                                                                                                                                                                        aCTGACTCATGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAGCTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTg                                                                  <  1:277490/300‑1 (MQ=255)                                                                                                                                                                                                                 ggctcTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTc                                                          >  2:447914/5‑299 (MQ=255)                                                                                                                                                                                                                   ctcTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTcgc                                                        >  1:274825/3‑299 (MQ=255)                                                                                                                                                                                                                            aGCGTAAAAAGACGCTGGCTGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGc                                              <  2:591709/300‑1 (MQ=255)                                                                                                                                                                                                                            aGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGc                                              <  1:38695/300‑1 (MQ=255)                                                                                                                                                                                                                             gCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCt                                             >  1:433375/1‑300 (MQ=255)                                                                                                                                                                                                                                    aaGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGc                                      <  1:471420/300‑1 (MQ=255)                                                                                                                                                                                                                                    aaGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGc                                      <  2:394049/300‑1 (MQ=255)                                                                                                                                                                                                                                           tGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCTAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCg                               <  2:396310/300‑1 (MQ=255)                                                                                                                                                                                                                                           tGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATgg                                 <  2:419767/298‑1 (MQ=255)                                                                                                                                                                                                                                           tGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCg                               <  1:530569/300‑1 (MQ=255)                                                                                                                                                                                                                                           tGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCg                               <  2:194434/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gaatCTTCCATCCAGCAAGGTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAg                    <  1:673906/297‑1 (MQ=255)                                                                                                                                                                                                                                                                        ggTCAGCAGCAGCTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCAACTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCAGGTTCGCGGGCCGAAGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGaaa  >  2:599382/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                           AGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAA  >  NC_000913/3787238‑3787800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑