Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,786,444	C→T	I395I (ATC→ATT)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,786,444	0	C	T	100.0%	257.1 / NA	75	I395I (ATC→ATT)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/48);  total (27/48)

GAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGT  >  NC_000913/3786148‑3786739                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        gAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCattatc                                                                                                                                                                                                                                                                                                      <  2:80794/300‑1 (MQ=255)  aatacgccacgtaCATCAAAACTGTGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAATGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCt                                                                                                                                                                                                                                                                                                     <  2:314395/288‑1 (MQ=255)  aaTACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCt                                                                                                                                                                                                                                                                                                     <  1:610572/300‑1 (MQ=255)  aaTACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCGTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCt                                                                                                                                                                                                                                                                                                     <  2:137394/300‑1 (MQ=255)       gccgcTGACATCAAAACTGCGTTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAAc                                                                                                                                                                                                                                                                                                <  2:338272/300‑1 (MQ=255)       gccgcTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTaa                                                                                                                                                                                                                                                                                                 <  1:199691/299‑1 (MQ=255)       gccgcTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAAc                                                                                                                                                                                                                                                                                                <  2:376435/300‑1 (MQ=255)       gccgcTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACAGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAAc                                                                                                                                                                                                                                                                                                <  1:17106/300‑1 (MQ=255)               cATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATAGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGNGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGaa                                                                                                                                                                                                                                                                                        >  2:497521/1‑300 (MQ=255)                           ggTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATgg                                                                                                                                                                                                                                                                             <  1:460115/299‑1 (MQ=255)                           ggTTGCTTACCCACCCGCTTCCCGGGTTAACACCTTCGGCGAGTGGATGGCGAAAAATGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGTCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATgg                                                                                                                                                                                                                                                                             <  2:155141/299‑1 (MQ=255)                           ggTTACTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTAGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGt                                                                                                                                                                                                                                                                            <  1:239870/300‑1 (MQ=255)                            gTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAGCCGAAAAATATGCCCACGTTACTTTCTTCTTAAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCCACCGGAAATGAGCTCCGCAGAGCTGACCGAAAGACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCCTTATCTTTAACTATCCGAACGGTGAAATGGTa                                                                                                                                                                                                                                                                           >  2:43951/1‑300 (MQ=255)                            gTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACATACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCACATACGACACCATTATCTGTAACTATCCGAACGGTGAAATGGTa                                                                                                                                                                                                                                                                           >  2:199636/1‑300 (MQ=255)                              tGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAgg                                                                                                                                                                                                                                                                         <  1:455687/300‑1 (MQ=255)                              tGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTAACTACGATCTGCAAACGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCAATCAAGAGAGGCAAAGACGAAACAATTAGCTGTAACTATCCGAACGGTGACATGGTa                                                                                                                                                                                                                                                                           >  2:354345/1‑298 (MQ=255)                               gCTTACCCACCCGCTTCCCCGGTTAACACCTTCTGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGTTGGCGTATAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTTGCTACCTACGATCTGCAACCGGAAATGAACTCCGCAGAGCTGACCGAAAAACTGGTTGCAGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACTTTGACATGGTAgg                                                                                                                                                                                                                                                                         <  2:345129/299‑1 (MQ=255)                                 ttatccacccGCTTCCNTGGTTAACACCTTCGGCGNGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAANGGTGACATGGTAGGTc                                                                                                                                                                                                                                                                       <  1:583686/295‑1 (MQ=255)                                 ttACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGTTGACATGGTAGGTc                                                                                                                                                                                                                                                                       <  1:295455/299‑1 (MQ=255)                                 ttACCCACCCGCTTCCCTGGTTAACACCTGCGGCGAGTGGATGGCGAAAAACGACAAAACTCATTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGTTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGTAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACCATCCGAACGGTGACATGGTAgggc                                                                                                                                                                                                                                                                       <  2:28578/299‑3 (MQ=255)                                   acccacccGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTcaca                                                                                                                                                                                                                                                                    >  1:619313/1‑300 (MQ=255)                                           gCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGt                                                                                                                                                                                                                                                             <  1:55930/299‑1 (MQ=255)                                           gCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAAGATGCCCACGGTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTATGATCTGCAACCGGAAGTGAACTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGGAGGTCACACCGGGGt                                                                                                                                                                                                                                                             <  2:313683/299‑1 (MQ=255)                                              tCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGAt                                                                                                                                                                                                                                                          >  1:271291/1‑299 (MQ=255)                                                   ggTTAACACCTTCGGCGGGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATAAGACACCATTATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCAGGGTGATGGaa                                                                                                                                                                                                                                                      >  2:16343/1‑298 (MQ=255)                                                   ggTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCATTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCTGCAAATACGACACCATTATCTGTAACTATCCGAACGTTGACATGGTAGGTCACACCGGGGTGATGGAAgc                                                                                                                                                                                                                                                    <  2:21926/300‑1 (MQ=255)                                                   ggTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAgc                                                                                                                                                                                                                                                    >  1:409109/1‑300 (MQ=255)                                                   ggTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAgc                                                                                                                                                                                                                                                    >  2:588263/1‑300 (MQ=255)                                                           ccTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTa                                                                                                                                                                                                                                            >  1:8968/1‑300 (MQ=255)                                                                ggCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGc                                                                                                                                                                                                                                        <  1:10798/299‑1 (MQ=255)                                                                ggCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACAGGGGTGATGGAAGCGGCGGTTAAAGc                                                                                                                                                                                                                                        >  2:396092/1‑299 (MQ=255)                                                                        gATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCCTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGTTGATGGAAGCGGCGGTTAAAGCGGTTGAAgc                                                                                                                                                                                                                               <  1:290387/300‑1 (MQ=255)                                                                          tGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAgcac                                                                                                                                                                                                                             <  1:679095/300‑3 (MQ=255)                                                                               aaaaaCGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGAt                                                                                                                                                                                                                        <  1:287593/300‑1 (MQ=255)                                                                               aaaaaCGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTGCAACGTTGGCGTATAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCGACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGTTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGAt                                                                                                                                                                                                                        <  2:660905/300‑1 (MQ=255)                                                                                  aaCGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATAGGCCCACGTTACTTTCTTCTTCAACGGTTGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGTAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAGGCGGTTGAAGCACTGGATCAc                                                                                                                                                                                                                     <  2:186122/300‑1 (MQ=255)                                                                                     gACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTg                                                                                                                                                                                                                   <  1:707266/299‑1 (MQ=255)                                                                                         aaaCTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGt                                                                                                                                                                                                                >  1:336668/1‑298 (MQ=255)                                                                                             tCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTTGCTACCTACGATCTGCAACCGTAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAATGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGgaag                                                                                                                                                                                                           <  2:344261/299‑1 (MQ=255)                                                                                             tCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGgaag                                                                                                                                                                                                           <  2:113022/299‑1 (MQ=255)                                                                                             tCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGgaag                                                                                                                                                                                                           <  1:183548/299‑1 (MQ=255)                                                                                                                       aaTATGCCCACGTGACTTGCTGCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCgtgg                                                                                                                                                                                <  2:130908/300‑1 (MQ=255)                                                                                                                        atatGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCgtggg                                                                                                                                                                               <  1:43951/300‑1 (MQ=255)                                                                                                                                                     gTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCAc                                                                                                                                                   >  2:433145/1‑299 (MQ=255)                                                                                                                                                       ggCGTAGAAGAGTCGTTCAAAGGCGAAGTTCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGt                                                                                                                                                >  1:61255/1‑300 (MQ=255)                                                                                                                                                        gCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTa                                                                                                                                               >  2:138487/1‑300 (MQ=255)                                                                                                                                                          gTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAAc                                                                                                                                             >  1:476451/1‑300 (MQ=255)                                                                                                                                                            agaagaGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGc                                                                                                                                           <  1:207632/300‑1 (MQ=255)                                                                                                                                                            agaagaGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACCGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGTTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGc                                                                                                                                           <  2:249240/300‑1 (MQ=255)                                                                                                                                                            agaagaGTCGGTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGc                                                                                                                                           <  2:597230/300‑1 (MQ=255)                                                                                                                                                                 agTCGTTCAAAGGCGAANATCGCATTCTGATCAACTNACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGANCACGGTAACGCTGaac                                                                                                                                      <  1:586751/300‑3 (MQ=255)                                                                                                                                                                 agTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGaac                                                                                                                                      <  1:240485/300‑3 (MQ=255)                                                                                                                                                                 agTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGaac                                                                                                                                      <  2:443225/300‑3 (MQ=255)                                                                                                                                                                 agTCGTTCAAAGGCGAAAATCGCATTCTGATCAACTCACCGAGAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGTTGACATGGTAGGTCACATCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCTTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGaac                                                                                                                                      <  1:240495/300‑3 (MQ=255)                                                                                                                                                                  gTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTTGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGaaca                                                                                                                                     <  2:305608/300‑4 (MQ=255)                                                                                                                                                                  gTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGaaca                                                                                                                                     <  2:302910/300‑4 (MQ=255)                                                                                                                                                                     gTTCAACGGCGAAGCTCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGACGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATTTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGCAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGATGAACAGa                                                                                                                                   >  2:470190/1‑299 (MQ=255)                                                                                                                                                                                   aTCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGANCGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCaa                                                                                                                    >  2:496755/1‑300 (MQ=255)                                                                                                                                                                                   aTCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCaa                                                                                                                    >  2:561308/1‑300 (MQ=255)                                                                                                                                                                                                    tCACCGAAAGTGGCTACCTACGATCTGCAACCGTAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTANAGCGGTTGAAGCACTGGATCACTGCGTTGAAGNAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTAATCACCGCTGACCACGGTAACGCTGAACAAATGCGCGATCCGGCAACGGGTCAggcacacac                                                                                                    <  2:527341/299‑1 (MQ=255)                                                                                                                                                                                                    tCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGTCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAggcacacac                                                                                                    <  2:424418/299‑1 (MQ=255)                                                                                                                                                                                                    tCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAggcacacac                                                                                                    <  1:121145/299‑1 (MQ=255)                                                                                                                                                                                                     cACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAggcacaca                                                                                                     >  2:437377/1‑297 (MQ=255)                                                                                                                                                                                                         gAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAggcacacacggcac                                                                                               <  1:627735/299‑1 (MQ=255)                                                                                                                                                                                                                  tacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAAcc                                                                                      <  1:22656/299‑1 (MQ=255)                                                                                                                                                                                                                            cTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTcc                                                                            >  2:457852/1‑299 (MQ=255)                                                                                                                                                                                                                                     gAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGGAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCCCGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAAGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTa                                                                   >  2:346789/1‑299 (MQ=255)                                                                                                                                                                                                                                       aaTGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTANCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGt                                                                <  1:497521/300‑1 (MQ=255)                                                                                                                                                                                                                                               ccGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTACGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACATACCCACCTGCCAGTTTCGCTGAtttcagtt                                                               >  2:83503/1‑288 (MQ=255)                                                                                                                                                                                                                                                 gCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAg                                                      <  1:199636/300‑1 (MQ=255)                                                                                                                                                                                                                                                   agagCTGACCGAAAAACTGGTTGCTGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACTGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAATCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGaa                                                    <  2:7539/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gcgCTGCCCGAAAACCTGGTTGCGGCCATCAAGAGCGGCAAATACGACACAATTATTTGTAACTATCCGAACGGTGACATGGTAGGTCACAAAGGGGTGATGGAAGCGGCGGTTAAAGCGGTTAAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACAGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCTGCTGCTTTACGTTGGTGAt                                                         >  2:118317/3‑294 (MQ=255)                                                                                                                                                                                                                                                    gagCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGNAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATNACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAAc                                                   >  2:532682/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gCCATCACGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGANCACCGCTGAACACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAAACTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGTGGTTGCAGGTGGCaac                           >  2:205420/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                 gACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCAAACTTTCTGACATCGCGCCGAcc      <  1:396092/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     ccATTATCTGCAACTATCCGAACGGTGCCATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTCGAAGCGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAACAGATGCACGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCAAATTCCGCTGATTTACGTTGGTGATAAGGACGTGAAAGCGGTTGCAGGCGGAAAGCTTTCTGACATCgcgcagaaaatgt  >  1:161410/1‑291 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        GAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGT  >  NC_000913/3786148‑3786739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑