Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,786,084	G→A	V275V (GTG→GTA)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,786,084	0	G	A	100.0%	248.1 / NA	80	V275V (GTG→GTA)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/33);  total (47/33)

GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAA  >  NC_000913/3785815‑3786378                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                       ggTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTa                                                                                                                                                                                                                                                                            >  1:251620/1‑298 (MQ=255) ggTCGTTACTAAGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTaa                                                                                                                                                                                                                                                                           >  1:380588/1‑299 (MQ=255)      ttACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGAGTAAGAAAGt                                                                                                                                                                                                                                                                     >  2:430152/1‑300 (MQ=255)      ttACTACGCGATGGACCGCGATAACCATTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCAAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTCCGACGGCTTCGCGCGTAAGaaatt                                                                                                                                                                                                                                                                     >  2:199691/1‑298 (MQ=255)        actacGCGATGGACCGCGATAACCGTTGGAATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCAGATACTGCAGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACAACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCAATCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGt                                                                                                                                                                                                                                                                     >  2:18325/1‑298 (MQ=255)         ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGt                                                                                                                                                                                                                                                                  >  1:185293/1‑300 (MQ=255)           acGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCNGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTa                                                                                                                                                                                                                                                                >  1:155141/1‑300 (MQ=255)             gcgATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAACCGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAAc                                                                                                                                                                                                                                                              >  2:116976/1‑300 (MQ=255)               gATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGt                                                                                                                                                                                                                                                            <  1:610635/300‑1 (MQ=255)                        cgATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGTCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGt                                                                                                                                                                                                                                                   >  1:660905/1‑300 (MQ=255)                        cgATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGt                                                                                                                                                                                                                                                   >  2:141929/1‑300 (MQ=255)                          aTAACCGTTGGGATCGCGTGGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGa                                                                                                                                                                                                                                                 <  2:415662/300‑1 (MQ=255)                             aCCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGc                                                                                                                                                                                                                                              <  1:59259/300‑1 (MQ=255)                               cGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCt                                                                                                                                                                                                                                             >  2:230193/1‑299 (MQ=255)                                           gttgAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCANCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATAcgcc                                                                                                                                                                                                                                <  1:493649/297‑1 (MQ=255)                                                  aagcctacgACCTGCTGACTCTTTCGCAGGGTGATTTCCAGTCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCGCGCGGCGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGTAAGACTGCGAGGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACACAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACa                                                                                                                                                                                                                         <  2:388363/292‑1 (MQ=255)                                                    gcctATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATc                                                                                                                                                                                                                       >  2:247587/4‑300 (MQ=255)                                                        aTGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGGGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCaaaa                                                                                                                                                                                                                   >  2:618126/1‑300 (MQ=255)                                                         tGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGANGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAAc                                                                                                                                                                                                                  <  1:141220/300‑1 (MQ=255)                                                          gACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTNCATGAACTTCCGTGCTGACCGCGCGCGCGAAANCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAAc                                                                                                                                                                                                                  <  2:522701/299‑1 (MQ=255)                                                            ccTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGCGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGc                                                                                                                                                                                                               >  1:344261/1‑300 (MQ=255)                                                                        ggCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTaccc                                                                                                                                                                                                   <  1:346075/300‑1 (MQ=255)                                                                        ggCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTGATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTaccc                                                                                                                                                                                                   <  1:336172/300‑1 (MQ=255)                                                                            cAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAACACTGCGGTTGCTTacc                                                                                                                                                                                                    >  2:55930/1‑295 (MQ=255)                                                                              gggtgAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTANGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGc                                                                                                                                                                                             >  1:137394/5‑300 (MQ=255)                                                                              gggtgAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGc                                                                                                                                                                                             >  1:376435/5‑300 (MQ=255)                                                                              gggtgAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGc                                                                                                                                                                                             >  2:455687/5‑300 (MQ=255)                                                                              gggtgAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGAAGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGAACGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacccaccc                                                                                                                                                                                               >  2:239870/5‑298 (MQ=255)                                                                                   aGTTCCATGCCAATACTGCCGTTGCTGTTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGGAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTccc                                                                                                                                                                                        <  2:453564/300‑1 (MQ=255)                                                                                    gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCt                                                                                                                                                                                       >  1:21926/1‑300 (MQ=255)                                                                                    gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCt                                                                                                                                                                                       >  2:679095/1‑300 (MQ=255)                                                                                    gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGCTGCTTACCCACCCGCTTCCCt                                                                                                                                                                                       >  1:345129/1‑300 (MQ=255)                                                                                    gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGAAGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAACACTGCGGTTGCTTACCCACCCGCTTCCCt                                                                                                                                                                                       >  2:583686/1‑300 (MQ=255)                                                                                    gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCATGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCt                                                                                                                                                                                       >  1:527341/1‑300 (MQ=255)                                                                                      tCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTgg                                                                                                                                                                                     <  1:24015/300‑1 (MQ=255)                                                                                         aGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGtt                                                                                                                                                                                   <  1:654827/299‑1 (MQ=255)                                                                                              gATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAacac                                                                                                                                                                              <  1:247587/299‑1 (MQ=255)                                                                                                tactgccgTTGTTGGTTTGCAGTCAGCTTATGTTCGCGACGGAAGTTACGAGTTCTTGAAATCGACCGTTATTCGTGTAGAAGGTCAGTCAGATGCGGCAATGGGAGACGGCGATGTGCTGATTTTCGTGAACTTTCGTGCTGACCGCGCGCGCGAAATCATTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGGTCGAATGCGCCGTTGGCATTAAGATTGCGGTTgtttgtctacc                                                                                                                                                                                                <  2:333240/275‑11 (MQ=255)                                                                                                  ctgccgTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGAAGATTTCGACGGCTTCGAGCGTAAGAAAGTGGTTAATGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTACATGGTTAACACCTTc                                                                                                                                                                          >  2:295455/3‑299 (MQ=255)                                                                                                       gTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAg                                                                                                                                                                    <  1:253940/300‑1 (MQ=255)                                                                                                             ggTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGAt                                                                                                                                                               >  1:443225/1‑299 (MQ=255)                                                                                                             ggTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGAt                                                                                                                                                               >  2:460115/1‑299 (MQ=255)                                                                                                                       cagctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAAc                                                                                                                                                     >  1:186122/3‑299 (MQ=255)                                                                                                                         gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGANGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAc                                                                                                                                                  <  2:502572/300‑1 (MQ=255)                                                                                                                         gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAc                                                                                                                                                  >  1:28578/1‑300 (MQ=255)                                                                                                                         gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAc                                                                                                                                                  >  2:707266/1‑300 (MQ=255)                                                                                                                         gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGATGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAc                                                                                                                                                  >  2:287593/1‑300 (MQ=255)                                                                                                                         gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGAAGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACACGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGa                                                                                                                                                   >  2:10798/1‑299 (MQ=255)                                                                                                                                          aaacgacgagttCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTAt                                                                                                                                 <  1:426199/290‑1 (MQ=255)                                                                                                                                                 gagttCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGTTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTccgaa                                                                                                                          >  1:338272/4‑300 (MQ=255)                                                                                                                                                      cGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTccgaaaccga                                                                                                                     >  1:203147/1‑300 (MQ=255)                                                                                                                                                                 ccGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCNTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGccc                                                                                                          >  1:130908/1‑300 (MQ=255)                                                                                                                                                                                  ggTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttcttctt                                                                                          >  2:183548/1‑299 (MQ=255)                                                                                                                                                                                          agatgctgCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGt                                                                                   <  1:249245/291‑1 (MQ=255)                                                                                                                                                                                                 gCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaa                                                                          <  1:351141/300‑1 (MQ=255)                                                                                                                                                                                                 gCAATGGAAGACGGCAATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGTGCGTAAGAAAGTGGTTAAGGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTCCTTCAACGGTGGCGTagaa                                                                          <  1:688165/300‑1 (MQ=255)                                                                                                                                                                                                   aatttaaGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag                                                                         <  2:380588/294‑1 (MQ=255)                                                                                                                                                                                                   aagggAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag                                                                         <  2:347402/296‑1 (MQ=255)                                                                                                                                                                                                   aaTGGAAGACTGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGGCGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTNCGGTTGCTTACCCACCCGCTTCCCTGTTTAACNCCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGGagaag                                                                         <  2:520072/299‑1 (MQ=255)                                                                                                                                                                                                   aaTGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCTTGATGCTGACCGAATACGCCGCTGACATCAAAACTNCGGTTGCTTACCCACCCGCTTCCCTGGTTAACNCCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag                                                                         <  2:520065/299‑1 (MQ=255)                                                                                                                                                                                                   aaTGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGGGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag                                                                         <  1:387746/299‑1 (MQ=255)                                                                                                                                                                                                   aaTGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag                                                                         <  1:373648/299‑1 (MQ=255)                                                                                                                                                                                                    aTGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaaga                                                                        >  1:424418/1‑299 (MQ=255)                                                                                                                                                                                                                gaggtgcTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTTGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCNACCCGCTTCCCTGGTTAACACCTTCGTCGAGTGGATGGCGAAAGACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTTGTTCaaggg                                                            <  2:494930/293‑4 (MQ=255)                                                                                                                                                                                                                       tGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGAt                                                     <  1:164573/299‑1 (MQ=255)                                                                                                                                                                                                                       tGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGGGCATTTGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGAt                                                     <  1:164474/299‑1 (MQ=255)                                                                                                                                                                                                                                  agcTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATc                                         <  1:224124/298‑1 (MQ=255)                                                                                                                                                                                                                                  aaCTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATc                                         <  1:222854/300‑1 (MQ=255)                                                                                                                                                                                                                                      tCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGCAACCGAAAAATATGCCCACGTTACTTTCTTCTTAAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGACGATCGCATTCTGATCAAc                                      >  2:121145/1‑299 (MQ=255)                                                                                                                                                                                                                                         gTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCAc                                  >  2:586751/1‑300 (MQ=255)                                                                                                                                                                                                                                         gTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTATGATCAACTCAc                                  >  2:240485/1‑300 (MQ=255)                                                                                                                                                                                                                                          tGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCAcc                                 >  2:627735/1‑300 (MQ=255)                                                                                                                                                                                                                                              gACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGaaa                             >  1:249240/1‑300 (MQ=255)                                                                                                                                                                                                                                              gACCGCGCGCGCGAAANCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTNAAAGGCGAAGATCGCATTNTGATCAACTCACCGaaa                             >  1:597230/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gcgcgcGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGct                       <  1:618126/300‑1 (MQ=255)                                                                                                                                                                                                                                                           aaaTCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGTTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAANGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGACCAACTCACCGaacgttgccccctac                 >  2:207632/1‑286 (MQ=255)                                                                                                                                                                                                                                                            aaTCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGGGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGTTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGAGGGCGAAAAACGACAAAACTCAGTTGCGTAGTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGctacc                    <  2:162378/295‑1 (MQ=255)                                                                                                                                                                                                                                                               cACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTACCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCt            >  1:157324/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCaa        >  2:22656/1‑299 (MQ=255)                                                                                                                                                                                                                                                                         ttCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGaa  >  1:7539/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                       GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAA  >  NC_000913/3785815‑3786378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑