Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,786,027	A→G	A256A (GCA→GCG)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,786,027	0	A	G	100.0%	303.7 / NA	85	A256A (GCA→GCG)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (52/33);  total (52/33)

GCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGA  >  NC_000913/3785736‑3786308                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                          gcgcAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGAtttt                                                                                                                                                                                                                                                                                   >  1:61439/1‑300 (MQ=255)      gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATACCCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGa                                                                                                                                                                                                                                                                              >  2:610572/1‑300 (MQ=255)      gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGCAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGa                                                                                                                                                                                                                                                                              >  2:253940/1‑300 (MQ=255)      gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGTAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGa                                                                                                                                                                                                                                                                              >  2:141220/1‑300 (MQ=255)      gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGa                                                                                                                                                                                                                                                                              >  1:106982/1‑300 (MQ=255)        gCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAAc                                                                                                                                                                                                                                                                            >  2:387746/1‑300 (MQ=255)        gCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAAc                                                                                                                                                                                                                                                                            >  1:347402/1‑300 (MQ=255)                 tCGCTGAAAAAATTTGAATAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGc                                                                                                                                                                                                                                                                    <  1:381792/299‑1 (MQ=255)                                   gagaaaTTTGCTGCGCAGGGTAAAGGCCGCTTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATc                                                                                                                                                                                                                                                 <  1:106382/297‑1 (MQ=255)                                   gagaaaTTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGaaacc                                                                                                                                                                                                                                                 <  1:106389/297‑3 (MQ=255)                                    agaaaTTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATc                                                                                                                                                                                                                                                 <  1:106730/297‑1 (MQ=255)                                    agaaaTTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATc                                                                                                                                                                                                                                                 <  1:616445/297‑1 (MQ=255)                                              tgcgcAGGGTAAAGGCCGCTTAGCGTCCATCAGTGGTCGCTACTACGCGATGGACCGCGATAACCGTTTGGATCGCGTTGAAAAAGCCTGTGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCTTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGATCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGcat                                                                                                                                                                                                                                       <  2:198117/298‑3 (MQ=255)                                              tgcgcAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTTGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATTCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGATCGCGCGCGCGAAAGCACTCGTGcat                                                                                                                                                                                                                                       <  2:198678/298‑3 (MQ=255)                                                    gggtagaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCACAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCAATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCACGCGCGAAATCACTCGTGCATTCGtaa                                                                                                                                                                                                                                 >  2:164474/7‑297 (MQ=255)                                                    gggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAANACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGACATCACTCGTGCATTCGtaaa                                                                                                                                                                                                                                >  2:222854/5‑297 (MQ=255)                                                    gggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAANACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTgtattcgtaaa                                                                                                                                                                                                                                >  2:224124/5‑290 (MQ=255)                                                    gggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGNAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGtaaa                                                                                                                                                                                                                                >  1:150722/5‑297 (MQ=255)                                                    gggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGGAATCACTAGTgtattcgtaaa                                                                                                                                                                                                                                >  2:173944/5‑290 (MQ=255)                                                    gggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGcat                                                                                                                                                                                                                                       >  2:164573/5‑291 (MQ=255)                                                    gggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGtaaa                                                                                                                                                                                                                                >  1:80794/5‑297 (MQ=255)                                                    gggtaaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTAAGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGTGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGcatccgtaaa                                                                                                                                                                                                                                >  2:17106/5‑291 (MQ=255)                                                                                ggTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTa                                                                                                                                                                                                      >  1:251620/1‑298 (MQ=255)                                                                                ggTCGTTACTAAGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTaa                                                                                                                                                                                                     >  1:380588/1‑299 (MQ=255)                                                                                     ttACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGAGTAAGAAAGt                                                                                                                                                                                               >  2:430152/1‑300 (MQ=255)                                                                                     ttACTACGCGATGGACCGCGATAACCATTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCAAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTCCGACGGCTTCGCGCGTAAGaaatt                                                                                                                                                                                               >  2:199691/1‑298 (MQ=255)                                                                                       actacGCGATGGACCGCGATAACCGTTGGAATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCAGATACTGCAGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACAACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCAATCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGt                                                                                                                                                                                               >  2:18325/1‑298 (MQ=255)                                                                                        ctacGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGt                                                                                                                                                                                            >  1:185293/1‑300 (MQ=255)                                                                                          acGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCNGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTa                                                                                                                                                                                          >  1:155141/1‑300 (MQ=255)                                                                                            gcgATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAACCGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAAc                                                                                                                                                                                        >  2:116976/1‑300 (MQ=255)                                                                                              gATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGt                                                                                                                                                                                      <  1:610635/300‑1 (MQ=255)                                                                                                       cgATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGTCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGt                                                                                                                                                                             >  1:660905/1‑300 (MQ=255)                                                                                                       cgATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGt                                                                                                                                                                             >  2:141929/1‑300 (MQ=255)                                                                                                         aTAACCGTTGGGATCGCGTGGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGa                                                                                                                                                                           <  2:415662/300‑1 (MQ=255)                                                                                                            aCCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGc                                                                                                                                                                        <  1:59259/300‑1 (MQ=255)                                                                                                              cGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCt                                                                                                                                                                       >  2:230193/1‑299 (MQ=255)                                                                                                                          gttgAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCANCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATAcgcc                                                                                                                                                          <  1:493649/297‑1 (MQ=255)                                                                                                                                 aagcctacgACCTGCTGACTCTTTCGCAGGGTGATTTCCAGTCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCGCGCGGCGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGTAAGACTGCGAGGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACACAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACa                                                                                                                                                   <  2:388363/292‑1 (MQ=255)                                                                                                                                   gcctATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATc                                                                                                                                                 >  2:247587/4‑300 (MQ=255)                                                                                                                                       aTGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGGGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCaaaa                                                                                                                                             >  2:618126/1‑300 (MQ=255)                                                                                                                                        tGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGANGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAAc                                                                                                                                            <  1:141220/300‑1 (MQ=255)                                                                                                                                         gACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTNCATGAACTTCCGTGCTGACCGCGCGCGCGAAANCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAAc                                                                                                                                            <  2:522701/299‑1 (MQ=255)                                                                                                                                           ccTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGCGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGc                                                                                                                                         >  1:344261/1‑300 (MQ=255)                                                                                                                                                       ggCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTaccc                                                                                                                             <  1:346075/300‑1 (MQ=255)                                                                                                                                                       ggCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTGATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTaccc                                                                                                                             <  1:336172/300‑1 (MQ=255)                                                                                                                                                           cAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAACACTGCGGTTGCTTacc                                                                                                                              >  2:55930/1‑295 (MQ=255)                                                                                                                                                             gggtgAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTANGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGc                                                                                                                       >  1:137394/5‑300 (MQ=255)                                                                                                                                                             gggtgAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGc                                                                                                                       >  1:376435/5‑300 (MQ=255)                                                                                                                                                             gggtgAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGc                                                                                                                       >  2:455687/5‑300 (MQ=255)                                                                                                                                                             gggtgAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGAAGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGAACGAATACGCCGCTGACATCAAAACTGCGGTTGCTTacccaccc                                                                                                                         >  2:239870/5‑298 (MQ=255)                                                                                                                                                                  aGTTCCATGCCAATACTGCCGTTGCTGTTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGGAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTccc                                                                                                                  <  2:453564/300‑1 (MQ=255)                                                                                                                                                                   gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCt                                                                                                                 >  2:679095/1‑300 (MQ=255)                                                                                                                                                                   gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCt                                                                                                                 >  1:21926/1‑300 (MQ=255)                                                                                                                                                                   gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGCTGCTTACCCACCCGCTTCCCt                                                                                                                 >  1:345129/1‑300 (MQ=255)                                                                                                                                                                   gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGAAGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAACACTGCGGTTGCTTACCCACCCGCTTCCCt                                                                                                                 >  2:583686/1‑300 (MQ=255)                                                                                                                                                                   gTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCATGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCt                                                                                                                 >  1:527341/1‑300 (MQ=255)                                                                                                                                                                     tCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTgg                                                                                                               <  1:24015/300‑1 (MQ=255)                                                                                                                                                                        aGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGtt                                                                                                             <  1:654827/299‑1 (MQ=255)                                                                                                                                                                             gATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAacac                                                                                                        <  1:247587/299‑1 (MQ=255)                                                                                                                                                                               tactgccgTTGTTGGTTTGCAGTCAGCTTATGTTCGCGACGGAAGTTACGAGTTCTTGAAATCGACCGTTATTCGTGTAGAAGGTCAGTCAGATGCGGCAATGGGAGACGGCGATGTGCTGATTTTCGTGAACTTTCGTGCTGACCGCGCGCGCGAAATCATTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGGTCGAATGCGCCGTTGGCATTAAGATTGCGGTTgtttgtctacc                                                                                                                          <  2:333240/275‑11 (MQ=255)                                                                                                                                                                                 ctgccgTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGAAGATTTCGACGGCTTCGAGCGTAAGAAAGTGGTTAATGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTACATGGTTAACACCTTc                                                                                                    >  2:295455/3‑299 (MQ=255)                                                                                                                                                                                      gTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAg                                                                                              <  1:253940/300‑1 (MQ=255)                                                                                                                                                                                            ggTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGAt                                                                                         >  1:443225/1‑299 (MQ=255)                                                                                                                                                                                            ggTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGAt                                                                                         >  2:460115/1‑299 (MQ=255)                                                                                                                                                                                                      cagctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAAc                                                                               >  1:186122/3‑299 (MQ=255)                                                                                                                                                                                                        gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGANGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAc                                                                            <  2:502572/300‑1 (MQ=255)                                                                                                                                                                                                        gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAc                                                                            >  2:707266/1‑300 (MQ=255)                                                                                                                                                                                                        gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAc                                                                            >  1:28578/1‑300 (MQ=255)                                                                                                                                                                                                        gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGATGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGAc                                                                            >  2:287593/1‑300 (MQ=255)                                                                                                                                                                                                        gctTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGAAGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACACGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGa                                                                             >  2:10798/1‑299 (MQ=255)                                                                                                                                                                                                                         aaacgacgagttCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTAt                                                           <  1:426199/290‑1 (MQ=255)                                                                                                                                                                                                                                gagttCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGTTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTccgaa                                                    >  1:338272/4‑300 (MQ=255)                                                                                                                                                                                                                                     cGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTccgaaaccga                                               >  1:203147/1‑300 (MQ=255)                                                                                                                                                                                                                                                ccGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCNTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGccc                                    >  1:130908/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 ggTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTttcttctt                    >  2:183548/1‑299 (MQ=255)                                                                                                                                                                                                                                                                         agatgctgCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGt             <  1:249245/291‑1 (MQ=255)                                                                                                                                                                                                                                                                                gCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaa    <  1:351141/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gCAATGGAAGACGGCAATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGTGCGTAAGAAAGTGGTTAAGGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTCCTTCAACGGTGGCGTagaa    <  1:688165/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aatttaaGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag   <  2:380588/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aagggAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag   <  2:347402/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aaTGGAAGACTGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGGCGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTNCGGTTGCTTACCCACCCGCTTCCCTGTTTAACNCCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGGagaag   <  2:520072/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aaTGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCTTGATGCTGACCGAATACGCCGCTGACATCAAAACTNCGGTTGCTTACCCACCCGCTTCCCTGGTTAACNCCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag   <  2:520065/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aaTGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGGGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag   <  1:387746/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aaTGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaag   <  1:373648/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aTGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGCGAAATCACTCGTGCATTCGTAAACGCAGATTTCGACGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTagaaga  >  1:424418/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                          GCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGTGAAATCACTCGTGCTTTCGTGAACGCTGATTTCGATGGCTTCGCGCGTAAGAAAGTGGTTAACGTCGATTTCGTGATGCTGACCGAATACGCCGCTGACATCAAAACTGCGGTTGCTTACCCACCCGCTTCCCTGGTTAACACCTTCGGCGAGTGGATGGCGAAAAACGACAAAACTCAGTTGCGTATTTCCGAAACCGAAAAATATGCCCACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGA  >  NC_000913/3785736‑3786308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑