Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,785,772 A→G E171E (GAA→GAG)  gpmM → 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,785,7720AG98.6% 253.3 / NA 73E171E (GAA→GAG) gpmM2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (37/34);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGC  >  NC_000913/3785488‑3786050
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                      
gtgtATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTgcgca                                                                                                                                                                                                                                                                          >  1:390795/1‑298 (MQ=255)
     tCAGGACCTGACTCTCCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTTAAGAGAAATTTGCTGCGCAgggtaa                                                                                                                                                                                                                                                                    <  2:128051/300‑4 (MQ=255)
     tCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAgggtaa                                                                                                                                                                                                                                                                    <  2:492653/300‑4 (MQ=255)
         gACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGc                                                                                                                                                                                                                                                                <  1:648080/300‑1 (MQ=255)
                  cGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTCCACCGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGTTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCACCGCGCAGTGCTGAATCTTCGCTGAACAAATTTGAAGAAAGAGTTGCTGCGCAGGGTAAAGGcaacgtaa                                                                                                                                                                                                                                                         >  2:336172/1‑291 (MQ=255)
                  cGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACNAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTAGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAACGGCAGCGTAGc                                                                                                                                                                                                                                                        >  2:493649/1‑299 (MQ=255)
                  cGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGc                                                                                                                                                                                                                                                        >  1:388363/1‑299 (MQ=255)
                           gtggAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCcatcat                                                                                                                                                                                                                                               <  1:208395/296‑1 (MQ=255)
                           gTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCcatcat                                                                                                                                                                                                                                               <  1:208450/299‑1 (MQ=255)
                           gTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCcatcat                                                                                                                                                                                                                                               <  2:493130/299‑1 (MQ=255)
                                aaTCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCCGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCg                                                                                                                                                                                                                                         <  2:366548/300‑1 (MQ=255)
                                         tCGCGCTTTCTTTGCTAATCCAGTGCTGACGGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCCCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCTACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAcgc                                                                                                                                                                                                                                <  2:252318/300‑1 (MQ=255)
                                             gcTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAGAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGAt                                                                                                                                                                                                                             <  1:664535/299‑1 (MQ=255)
                                             gcTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGAt                                                                                                                                                                                                                             <  1:664543/299‑1 (MQ=255)
                                                     tGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAAGCGATGGACCGCGa                                                                                                                                                                                                                    >  2:610635/1‑300 (MQ=255)
                                                     tGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCCCAGCAGGCGGCGTACACAGCCACGACGATCACATCACGGAGATGGTAGAACTGGCAGCTGAACGCGGCGAAGAAAAAATCTACCTGCACGCATTCATTGACGGTCGCGCCACCCCGCCGCGCAGTGCTGAATCCTAGCTGAAAGAATTTGAAGAGAAATTTGCTGCGCAGGGTCAAGGCAGCGTAGCGTCAATTCCTGGTCGTtact                                                                                                                                                                                                                                    >  2:610645/1‑284 (MQ=255)
                                                          aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACATCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAAcc                                                                                                                                                                                                               >  1:627392/1‑300 (MQ=255)
                                                          aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAATAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAcgc                                                                                                                                                                                                                                <  2:348376/283‑1 (MQ=255)
                                                          aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGc                                                                                                                                                                                                                                                                <  2:269582/251‑1 (MQ=255)
                                                          aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGc                                                                                                                                                                                                                                                                >  1:269582/1‑251 (MQ=255)
                                                          aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAcgc                                                                                                                                                                                                                                >  1:348376/1‑283 (MQ=255)
                                                               gTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCATGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCCGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCTATCATCGGTCGTTACTAAGCGATGGAATACGATAACCGtt                                                                                                                                                                                                            >  2:59259/1‑298 (MQ=255)
                                                               gTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGtt                                                                                                                                                                                                            >  1:415662/1‑298 (MQ=255)
                                                               gTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGCACACATTATGGGTCTGCTCTCCTCAGGCGGCGTACACAGCCAAGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGTTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTAGTCGTTACTACGCGATGGACCGCGATAACCGtt                                                                                                                                                                                                            >  1:333240/1‑298 (MQ=255)
                                                                 gCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACATCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGa                                                                                                                                                                                                        <  2:627392/300‑1 (MQ=255)
                                                                   tGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGTCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGAt                                                                                                                                                                                                       <  1:159143/299‑1 (MQ=255)
                                                                   tGACTGGCGCAGTAGATAAAGCTAAAAACGCAGGTAAAGCAGTACACATCATGGTTCTGCTCTCCGCAGGCGGCGTACACATCCACGAAGATCACATCATGTCGATGTTAGAACGGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCGGGACGGTTGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGAt                                                                                                                                                                                                       <  2:352215/299‑1 (MQ=255)
                                                                    gACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTGAAGCAGTACACATTATTGGTCTGCTCTCCGCAGGCGGCTTACACAGCCACGAAGATCACATCATGGCGATGGTAGGACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCGTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTTAAAAAATTTGAATAGAAATTTGCTGCGCAGGGTAAATGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGAt                                                                                                                                                                                                       <  2:182600/298‑1 (MQ=255)
                                                                         gcgcGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTAACTGCACGCATTCCTTGACGATCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAGTTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATACCCGTTGGGATCGCGt                                                                                                                                                                                                  >  2:346075/1‑298 (MQ=255)
                                                                           gcgGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGaa                                                                                                                                                                                              >  1:343129/1‑300 (MQ=255)
                                                                                       gCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGAc                                                                                                                                                                                  <  2:566533/300‑1 (MQ=255)
                                                                                         gAAAAACGCAGTTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCAGGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTTAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCACGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGAcc                                                                                                                                                                                 <  2:682812/299‑1 (MQ=255)
                                                                                                         gcagTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCAAGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAACAAGCCTATGACCTACTGACTCTGGCGCa                                                                                                                                                                 >  2:373648/4‑299 (MQ=255)
                                                                                                             tACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAgggtg                                                                                                                                                            <  2:574760/300‑3 (MQ=255)
                                                                                                                 caTTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGtt                                                                                                                                                        >  2:24015/1‑300 (MQ=255)
                                                                                                                  aTTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTATAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAGTAGAAATTTGCTGCGCAGGTTAAAGGCCGCTTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGtt                                                                                                                                                        <  2:178489/299‑1 (MQ=255)
                                                                                                                  aTTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTc                                                                                                                                                       <  2:465465/300‑1 (MQ=255)
                                                                                                                   ttATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTcc                                                                                                                                                      >  2:654827/1‑300 (MQ=255)
                                                                                                                    tATGGTTCTGCTCTCCGCGGGCGGCGTACACAGCAACGAAGATCACATCACTGCGATGGTAGAACTGGCAGCTGAACGCTGCGCAGAAAAAATCTACCTGCACGCATTCCTCGACGTTCGCGACACCCCGTCGCGCATTGCTGAAGCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTATGCGATGGACCGCGGTAACCGTTGGGATCGCGTTGAAAAAGCCTATTACCGGCTGGTTCTGGCGCAGGGTGAGTTc                                                                                                                                                       <  2:99066/298‑1 (MQ=255)
                                                                                                                       gggTCTGCTTTTCGCAGGCGGCGTACACAGCCACGAAAATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCACAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGc                                                                                                                                                  <  2:106171/300‑1 (MQ=255)
                                                                                                                        ggTCTGCTCTCCGCAGGCGGCGTACATAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCATTGACGGTCGAGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCTATCCTTGGTCGTTACTACGCGAt                                                                                                                                                                                                                             >  2:249245/1‑224 (MQ=255)
                                                                                                                        ggTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGcc                                                                                                                                                 >  1:452374/1‑300 (MQ=255)
                                                                                                                          tCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGcc                                                                                                                                                 <  2:343129/298‑1 (MQ=255)
                                                                                                                             gCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGNCCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGCTAc                                                                                                                                            >  1:494930/1‑300 (MQ=255)
                                                                                                                             gCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGNCCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATAc                                                                                                                                            >  1:494945/1‑300 (MQ=255)
                                                                                                                             gCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATAc                                                                                                                                            <  2:452374/300‑1 (MQ=255)
                                                                                                                                          ggcgTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGt                                                                                                                               >  1:522701/1‑300 (MQ=255)
                                                                                                                                          ggcgTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGNTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGt                                                                                                                               >  1:502572/1‑300 (MQ=255)
                                                                                                                                          ggcgTACACAGCAACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGt                                                                                                                               >  1:453564/1‑300 (MQ=255)
                                                                                                                                             gTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGttt                                                                                                                             >  2:426199/1‑299 (MQ=255)
                                                                                                                                                  caGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGTATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCAGTTGCTGGTTTGCAGgc                                                                                                                       >  1:520065/1‑300 (MQ=255)
                                                                                                                                                  caGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGgc                                                                                                                       >  1:520072/1‑300 (MQ=255)
                                                                                                                                                            gATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCATGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCt                                                                                                             <  2:390795/300‑1 (MQ=255)
                                                                                                                                                            gATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCt                                                                                                             <  1:196569/300‑1 (MQ=255)
                                                                                                                                                             aTCACATCATGGCAATGGTAGAACTGGCAGCTGAGCTCGGCGCATAAAAAATCTACCTGCACGCATTCCTTGACGTTCGCGACACCCTGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGGAGTGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGGGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCAGCTTATGCt                                                                                                             <  2:358281/299‑1 (MQ=255)
                                                                                                                                                             aTCACATCATGGCAATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTc                                                                                                            <  2:270786/300‑1 (MQ=255)
                                                                                                                                                                acaTCATTGCGATGTTAGAACTGGCAGCTGAACGCGGCGCAGAAGAAATCTACCTGCACGCATTCCTTGACGGTCGCTACACCCCGCCGCGCAGTGCGGAATCCTCGCGGAAAAAATTTGAAGAGGGATTTGCTACGCAGGGTAAAGGCCGCGTAGTATCCATCATTAGCCGTCACTACGCGATGGACCGCTATAACCGTTGGGATCGCGTTGAAAAAGCCTATTACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGTCGATACTGCCGTTGCTGGTTTGCAGTCAGCTTATGcc                                                                                                             <  2:358897/296‑2 (MQ=255)
                                                                                                                                                                                      ggCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGaa                                                                                   <  1:374639/300‑1 (MQ=255)
                                                                                                                                                                                                             aaaTCTACCTGCACGCGTTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTTAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCNTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAg                                                            <  2:505472/300‑1 (MQ=255)
                                                                                                                                                                                                                              ttCCTTGACGGTCGCGACCCCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAAAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAAGCGATGGAACGCGATAACCGTTGGGATCGCGTTGAAAAAGCATATGACCTGCTGACTCCGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCAAGATGCggaa                                           >  2:688165/1‑298 (MQ=255)
                                                                                                                                                                                                                              ttCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCa                                           >  1:439775/1‑300 (MQ=255)
                                                                                                                                                                                                                                        gTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGCCAGCCAGATGCGGCAATGGAAGAc                                  >  1:162378/1‑299 (MQ=255)
                                                                                                                                                                                                                                             gACACCCCGCCGCTCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGATAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGc                               <  2:20558/297‑1 (MQ=255)
                                                                                                                                                                                                                                             gACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTTAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCTTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCATGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGa                             <  1:536966/299‑1 (MQ=255)
                                                                                                                                                                                                                                                        gcgcAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGAtttt                 >  1:61439/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTTATGCGAAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAAGAAATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAAGGTGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCAGCTTATGCTTGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCTTGTAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGGTGcgtcggttttccgta            >  2:113178/1‑286 (MQ=255)
                                                                                                                                                                                                                                                             gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATACCCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGa            >  2:610572/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGCAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGa            >  2:253940/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGTAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGa            >  2:141220/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             gTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGa            >  1:106982/1‑300 (MQ=255)
                                                                                                                                                                                                                                                               gCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAAc          >  2:387746/1‑300 (MQ=255)
                                                                                                                                                                                                                                                               gCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAAc          >  1:347402/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                        tCGCTGAAAAAATTTGAATAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGACCTGCTGACTCTGGCGCAGGGTGAGTTCCAGGCCGATACTGCCGTTGCTGGTTTGCAGGCAGCTTATGCTCGCGACGAAAACGACGAGTTCGTGAAAGCGACCGTTATTCGTGCAGAAGGTCAGCCAGATGCGGCAATGGAAGACGGCGATGCGCTGATTTTCATGAACTTCCGTGc  <  1:381792/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                      
GTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGC  >  NC_000913/3785488‑3786050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑