Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,785,595	G→A	A112A (GCG→GCA)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,785,595	0	G	A	100.0%	294.6 / NA	84	A112A (GCG→GCA)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/44);  total (40/44)

ATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACC  >  NC_000913/3785300‑3785875                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                         atggctatggctATCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTac                                                                                                                                                                                                                                                                                      <  1:415342/300‑1 (MQ=255)  tggctatggctaTCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTaca                                                                                                                                                                                                                                                                                     >  1:270786/1‑300 (MQ=255)   ggctatggctatCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTacac                                                                                                                                                                                                                                                                                    >  1:566533/1‑300 (MQ=255)    gctatggctatCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCATTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGCCCTGACTCGTCTGGACGTTGCAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTAcaca                                                                                                                                                                                                                                                                                   >  1:682812/1‑300 (MQ=255)    gctatggctatCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTAcaca                                                                                                                                                                                                                                                                                   >  1:182600/1‑300 (MQ=255)    gctatggctatCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTAcaca                                                                                                                                                                                                                                                                                   >  2:245736/1‑300 (MQ=255)            tatCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGTTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGNGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATggg                                                                                                                                                                                                                                                                            <  2:214164/299‑1 (MQ=255)            tatCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATggg                                                                                                                                                                                                                                                                            <  1:518479/299‑1 (MQ=255)             atCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATggg                                                                                                                                                                                                                                                                            <  2:163175/298‑1 (MQ=255)             atCGTGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTc                                                                                                                                                                                                                                                                          <  1:630919/300‑1 (MQ=255)                     gaaCAGCAGGATAAAGCCATTTTTAGTGCTAAAACCAAGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTACGGCCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCCCGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTAGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGCTCTGctccca                                                                                                                                                                                                                                                                  >  2:374639/1‑297 (MQ=255)                        cagcagGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATTGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTcccca                                                                                                                                                                                                                                                               >  1:252318/1‑297 (MQ=255)                             ggATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAggc                                                                                                                                                                                                                                                            >  1:178489/1‑298 (MQ=255)                             ggATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTAAACATTATGGGTCTGCTCTCCGCAggc                                                                                                                                                                                                                                                            >  1:352215/1‑298 (MQ=255)                             ggATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCAGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAggc                                                                                                                                                                                                                                                            >  2:106382/1‑298 (MQ=255)                             ggATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTACGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCAAGTTAACCTGGGTGCCGGCCGCATCGTGTATTAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCTAGTGCTGAATGGCGCGGTAGATAAAGCGAAAAACGTAGGTAAAGCAGTa                                                                                                                                                                                                                                                                                       >  2:106389/1‑271 (MQ=255)                                  aCGCCATTTTTAGGGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTac                                                                                                                                                                                                                                                     <  2:598194/300‑1 (MQ=255)                                    gCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTNTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTacac                                                                                                                                                                                                                                                   <  1:510454/300‑1 (MQ=255)                                    gCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTacac                                                                                                                                                                                                                                                   <  1:245736/300‑1 (MQ=255)                                       aTTTTTAGTGCTAAAACCCCGTTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGNCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGc                                                                                                                                                                                                                                                <  2:556002/300‑1 (MQ=255)                                       aTTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGTGCCAATCGTCCGCATACCCTGATCGACGCTTCCAGTCGGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGc                                                                                                                                                                                                                                                <  2:256735/300‑1 (MQ=255)                                       aTTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTAcaca                                                                                                                                                                                                                                                  <  1:47594/298‑1 (MQ=255)                                                 ctcacaCCCCGGTCATGGATGCACTGTGGGCCAATCGTCCGCATCCCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTNCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCNCATCGTGTATCAGGACCTGAATCGTCTGAACGTTGACATCAAAGATCGCGCTTTCTTTGCTAATCCCGTGTTGACCGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACCCATTATGGGTCTGCTCTCCGCAGACGGCGTACACAGcacagcagatc                                                                                                                                                                                                                                      >  2:536966/6‑290 (MQ=255)                                                     aaCCCCGGTAATGGATNCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCNATCCGCaa                                                                                                                                                                                                                                                              >  1:579274/1‑271 (MQ=255)                                                      aCCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTATGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCAcatc                                                                                                                                                                                                                                 <  2:307933/300‑1 (MQ=255)                                                            gTAATGGATGCACTGTGNGCCAATCGTCCGCATACCNTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGNAGATCACATCATGGCg                                                                                                                                                                                                                           <  1:585387/300‑1 (MQ=255)                                                            gTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTATGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATgg                                                                                                                                                                                                                             <  2:458997/298‑1 (MQ=255)                                                            gTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGc                                                                                                                                                                                                                            >  1:465465/1‑299 (MQ=255)                                                            gTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGAAGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGAGTCTGCTCTCAGCAGGCGGCGTACACAGCAACGAAGATCACATCATGGc                                                                                                                                                                                                                            >  1:99066/1‑299 (MQ=255)                                                                tGGATGTACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGAGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGACAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCACAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGAt                                                                                                                                                                                                                         >  1:358281/1‑298 (MQ=255)                                                                tGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGAtt                                                                                                                                                                                                                        >  1:157077/1‑298 (MQ=255)                                                                tGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGAt                                                                                                                                                                                                                         >  1:20558/1‑298 (MQ=255)                                                                tGGATGCACTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCAAAAAGATCACATCATGGCGAt                                                                                                                                                                                                                         >  1:358897/1‑298 (MQ=255)                                                                        cTGTGGGCCAATCGTCCGCATACCCTGATCGACGCTTACGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGTGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTATGCTCTCCGCAGGCGGCGTACACAGCCAAGAAGATCACATCATGGCGATGGTAGAACt                                                                                                                                                                                                                >  2:616445/1‑299 (MQ=255)                                                                          gtgGGCCAATCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGc                                                                                                                                                                                                             <  1:611312/300‑1 (MQ=255)                                                                                 aaTCGTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTg                                                                                                                                                                                                        <  1:490193/298‑1 (MQ=255)                                                                                     gTCCGCATACCCTGATCTACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCTAAGTAGGCCACTTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAAcgc                                                                                                                                                                                                   <  2:285571/299‑1 (MQ=255)                                                                                     gTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAAcgc                                                                                                                                                                                                   <  1:212625/299‑1 (MQ=255)                                                                                     gTCCGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTAACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAAcgc                                                                                                                                                                                                   <  2:73268/299‑1 (MQ=255)                                                                                     gTCCGCATACCCTGATCGACGCGTCCTGTCTGGAAGTCGGTTTGCCGTACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAAGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTACTAATCCAGTGCTGACTTGCTCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGGACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACCGGCAGCTGAAcgc                                                                                                                                                                                                   <  2:285760/299‑1 (MQ=255)                                                                                        cGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCAACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACgcggc                                                                                                                                                                                                >  2:664543/1‑299 (MQ=255)                                                                                        cGCATACCCTGATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGCATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACgcggc                                                                                                                                                                                                >  2:196569/1‑299 (MQ=255)                                                                                        cGCATACCCTGATCGACGCTTACGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACgcggc                                                                                                                                                                                                >  2:664535/1‑299 (MQ=255)                                                                                                 tgaTCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATTGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATTGTAGAACTGGCAGCTGAACGCGGCGCAGaaa                                                                                                                                                                                         <  2:157077/295‑1 (MQ=255)                                                                                                                                                    aCTCCGAAGTAGGCCACGTTAACCTGTGTGCCTGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTNTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTg                                                                                                                                   <  2:496534/300‑1 (MQ=255)                                                                                                                                                    aCTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCTTCTGTACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTNTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGTCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTg                                                                                                                                   <  2:496527/300‑1 (MQ=255)                                                                                                                                                         gAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAAt                                                                                                                                <  1:74920/298‑1 (MQ=255)                                                                                                                                                         gAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATcc                                                                                                                              <  1:559872/300‑1 (MQ=255)                                                                                                                                                                   aCGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGaaaa                                                                                                                    >  1:198678/1‑300 (MQ=255)                                                                                                                                                                   aCGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTAACTGCACGCATTCCTTGACGGTCGCGACACCCCACCGCGCAGTGCTGAATCCTCGCTGaaaa                                                                                                                    >  1:198117/1‑300 (MQ=255)                                                                                                                                                                    cGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCAAGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCAGCGCAGTGCTGAATCCTCGCTGaaaaa                                                                                                                   >  2:381792/1‑300 (MQ=255)                                                                                                                                                                             gggTGCCGGCCGCATCGGGTATCAGGACCCGACTCGGCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGTCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGTTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATctgtctcttatacacatctcgc                                                                                                          <  1:246417/300‑22 (MQ=255)                                                                                                                                                                                             gtgtATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTgcgca                                                                                           >  1:390795/1‑298 (MQ=255)                                                                                                                                                                                                  tCAGGACCTGACTCTCCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTTAAGAGAAATTTGCTGCGCAgggtaa                                                                                     <  2:128051/300‑4 (MQ=255)                                                                                                                                                                                                  tCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAgggtaa                                                                                     <  2:492653/300‑4 (MQ=255)                                                                                                                                                                                                      gACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGc                                                                                 <  1:648080/300‑1 (MQ=255)                                                                                                                                                                                                               cGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTCCACCGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGTTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCACCGCGCAGTGCTGAATCTTCGCTGAACAAATTTGAAGAAAGAGTTGCTGCGCAGGGTAAAGGcaacgtaa                                                                          >  2:336172/1‑291 (MQ=255)                                                                                                                                                                                                               cGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACNAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTAGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAACGGCAGCGTAGc                                                                         >  2:493649/1‑299 (MQ=255)                                                                                                                                                                                                               cGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGc                                                                         >  1:388363/1‑299 (MQ=255)                                                                                                                                                                                                                        gtggAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCcatcat                                                                <  1:208395/296‑1 (MQ=255)                                                                                                                                                                                                                        gTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCcatcat                                                                <  1:208450/299‑1 (MQ=255)                                                                                                                                                                                                                        gTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCcatcat                                                                <  2:493130/299‑1 (MQ=255)                                                                                                                                                                                                                             aaTCAAAGATCGCGCTTTCTTTGCTAATCCAGTGCCGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCg                                                          <  2:366548/300‑1 (MQ=255)                                                                                                                                                                                                                                      tCGCGCTTTCTTTGCTAATCCAGTGCTGACGGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCCCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCTACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAcgc                                                 <  2:252318/300‑1 (MQ=255)                                                                                                                                                                                                                                          gcTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAGAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGAt                                              <  1:664535/299‑1 (MQ=255)                                                                                                                                                                                                                                          gcTTTCTTTGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGAt                                              <  1:664543/299‑1 (MQ=255)                                                                                                                                                                                                                                                  tGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAAGCGATGGACCGCGa                                     >  2:610635/1‑300 (MQ=255)                                                                                                                                                                                                                                                  tGCTAATCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCCCAGCAGGCGGCGTACACAGCCACGACGATCACATCACGGAGATGGTAGAACTGGCAGCTGAACGCGGCGAAGAAAAAATCTACCTGCACGCATTCATTGACGGTCGCGCCACCCCGCCGCGCAGTGCTGAATCCTAGCTGAAAGAATTTGAAGAGAAATTTGCTGCGCAGGGTCAAGGCAGCGTAGCGTCAATTCCTGGTCGTtact                                                     >  2:610645/1‑284 (MQ=255)                                                                                                                                                                                                                                                       aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACATCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAAcc                                >  1:627392/1‑300 (MQ=255)                                                                                                                                                                                                                                                       aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAATAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAcgc                                                 <  2:348376/283‑1 (MQ=255)                                                                                                                                                                                                                                                       aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGc                                                                                 >  1:269582/1‑251 (MQ=255)                                                                                                                                                                                                                                                       aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGc                                                                                 <  2:269582/251‑1 (MQ=255)                                                                                                                                                                                                                                                       aTCCAGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTAcgc                                                 >  1:348376/1‑283 (MQ=255)                                                                                                                                                                                                                                                            gTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCATGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCCGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCTATCATCGGTCGTTACTAAGCGATGGAATACGATAACCGtt                             >  2:59259/1‑298 (MQ=255)                                                                                                                                                                                                                                                            gTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGtt                             >  1:415662/1‑298 (MQ=255)                                                                                                                                                                                                                                                            gTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGCACACATTATGGGTCTGCTCTCCTCAGGCGGCGTACACAGCCAAGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGTTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTAGTCGTTACTACGCGATGGACCGCGATAACCGtt                             >  1:333240/1‑298 (MQ=255)                                                                                                                                                                                                                                                              gCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACATCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGa                         <  2:627392/300‑1 (MQ=255)                                                                                                                                                                                                                                                                tGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGTCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGAt                        <  1:159143/299‑1 (MQ=255)                                                                                                                                                                                                                                                                tGACTGGCGCAGTAGATAAAGCTAAAAACGCAGGTAAAGCAGTACACATCATGGTTCTGCTCTCCGCAGGCGGCGTACACATCCACGAAGATCACATCATGTCGATGTTAGAACGGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCGGGACGGTTGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGAt                        <  2:352215/299‑1 (MQ=255)                                                                                                                                                                                                                                                                 gACTGGCGCGGTAGATAAAGCGAAAAACGCAGGTGAAGCAGTACACATTATTGGTCTGCTCTCCGCAGGCGGCTTACACAGCCACGAAGATCACATCATGGCGATGGTAGGACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCGTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTTAAAAAATTTGAATAGAAATTTGCTGCGCAGGGTAAATGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGAt                        <  2:182600/298‑1 (MQ=255)                                                                                                                                                                                                                                                                      gcgcGGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTAACTGCACGCATTCCTTGACGATCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAGTTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATACCCGTTGGGATCGCGt                   >  2:346075/1‑298 (MQ=255)                                                                                                                                                                                                                                                                        gcgGTAGATAAAGCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGaa               >  1:343129/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gCGAAAAACGCAGGTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGAc   <  2:566533/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gAAAAACGCAGTTAAAGCAGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCAGGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACCCCGCCGCGCAGTGCTGAATCCTCGCTTAAAAAATTTGAAGAGAAATTTGCTGCGCAGGGTAAAGGCCACGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTTGAAAAAGCCTATGAcc  <  2:682812/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                         ATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGACTGGCGCGGTAGATAAAGCGAAAAACGCAGGCAAAGCGGTACACATTATGGGTCTGCTCTCCGCAGGCGGCGTACACAGCCACGAAGATCACATCATGGCGATGGTAGAACTGGCAGCTGAACGCGGCGCAGAAAAAATCTACCTGCACGCATTCCTTGACGGTCGCGACACTCCGCCGCGCAGTGCTGAATCCTCGCTGAAAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACC  >  NC_000913/3785300‑3785875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑