Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,216	G→A	Q152* (CAG→TAG)	ftsP ←	cell division protein FtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,216	0	G	A	100.0%	213.5 / NA	63	Q152* (CAG→TAG)	ftsP	cell division protein FtsP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/44);  total (19/44)

ACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCAC  >  NC_000913/3161923‑3162509                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      aCCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGTTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGc                                                                                                                                                                                                                                                                                                 <  2:445165/300‑1 (MQ=255) aCCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGc                                                                                                                                                                                                                                                                                                 <  1:123463/300‑1 (MQ=255)             cTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGNCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGtt                                                                                                                                                                                                                                                                                        >  1:517198/1‑297 (MQ=255)               gTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAg                                                                                                                                                                                                                                                                                   <  1:66413/300‑1 (MQ=255)                tAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGt                                                                                                                                                                                                                                                                                  <  1:673616/300‑1 (MQ=255)                tAGTTGATAGCGACGAGAGTTCGACGCATTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGt                                                                                                                                                                                                                                                                                  <  2:619639/300‑1 (MQ=255)                 aGTTGATAGCGATGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCTAGACTTCAACGTACGGTCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGGTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACAGTCCGGCAAGGCCGTTATAGACCTACTTAGCCGTGCGGTTGGGAGt                                                                                                                                                                                                                                                                                  <  2:355176/299‑1 (MQ=255)                     gATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGTGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCTGTTCGTGGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGTGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTgg                                                                                                                                                                                                                                                                             <  2:675630/300‑1 (MQ=255)                     gATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTgg                                                                                                                                                                                                                                                                             <  1:527102/300‑1 (MQ=255)                     gATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTGATAGACCTACTGAGCCGTGCGGTTGGGAGTATTgg                                                                                                                                                                                                                                                                             <  1:527115/300‑1 (MQ=255)                       tAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCg                                                                                                                                                                                                                                                                           <  2:404987/300‑1 (MQ=255)                                gagTTCGAAGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCTAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAACCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCTGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACcac                                                                                                                                                                                                                                                                  <  1:349282/300‑1 (MQ=255)                                  gTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGNAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGTGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACag                                                                                                                                                                                                                                                                <  1:154729/300‑1 (MQ=255)                                      gACGCGTTCAGCAGTCNCAAGCGCACCCAGCCACGNGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGNCGTGATACCACAGAGt                                                                                                                                                                                                                                                             <  1:592486/299‑1 (MQ=255)                                         gcgTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAACCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCt                                                                                                                                                                                                                                                         >  2:45640/1‑300 (MQ=255)                                         gcgTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCAGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCAGTGCGGTTGGGAGTATTGGCGTGATACCAAAGAGTAGCt                                                                                                                                                                                                                                                         >  2:45586/1‑300 (MQ=255)                                           gTTCAGCAGTCGCAAGCGCACCCAGCCACTCTAGACTTCAACGTACGTGCTTTATACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGTTTCGTTGTATTCTTGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCGCACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCATCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTg                                                                                                                                                                                                                                                        <  2:398896/299‑1 (MQ=255)                                           gTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTg                                                                                                                                                                                                                                                        <  2:399201/299‑1 (MQ=255)                                            ttCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTACAGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTCATAGACCTACTGAGCCGTGCGGTTGGGATTATTGGTGTGATACCACAGAGTAGCTGc                                                                                                                                                                                                                                                       >  2:101578/1‑299 (MQ=255)                                                    tCGCAAGCGCACCCAGCCACGCTAGACTTCAACTTACGTGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTGTCCTGGATAATGACCGGAAAATCATCCACACCATAATGTTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTggtg                                                                                                                                                                                                                                              <  2:698248/300‑3 (MQ=255)                                                    tCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCg                                                                                                                                                                                                                                              <  2:698781/300‑1 (MQ=255)                                                                   gCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                                                                                                                                                                                                               <  1:329718/300‑1 (MQ=255)                                                                      aCGCTAGACTTCAACGTACGGTCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTg                                                                                                                                                                                                                             <  2:33687/299‑1 (MQ=255)                                                                      aCGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGc                                                                                                                                                                                                                            >  1:606142/1‑300 (MQ=255)                                                                                          ggCTTTGTACACCGTTAACCAGCAGCGTATCACCCACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCAACAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAca                                                                                                                                                                                                        >  1:414508/1‑300 (MQ=255)                                                                                          ggCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAca                                                                                                                                                                                                        >  1:414398/1‑300 (MQ=255)                                                                                          ggCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTCGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTACGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGa                                                                                                                                                                                                          >  2:597114/1‑298 (MQ=255)                                                                                              ttGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCAGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatcat                                                                                                                                                                                                    >  2:572016/1‑300 (MQ=255)                                                                                                     ccGTTAACCAGCAGCGNATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTTGCGAATGGGCAGTACNGGTGCCCAGTCAGCGTTTNGCGACATCATCCGTGcc                                                                                                                                                                                             >  1:587621/1‑300 (MQ=255)                                                                                                         tAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGNGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGTCGACATCATCCGTGCCGGAc                                                                                                                                                                                         <  2:224494/300‑1 (MQ=255)                                                                                                                    gTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAg                                                                                                                                                                               <  1:290945/299‑1 (MQ=255)                                                                                                                              caaAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCNGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTa                                                                                                                                                                    >  1:511040/1‑300 (MQ=255)                                                                                                                                aaGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTAcc                                                                                                                                                                  <  1:527312/300‑1 (MQ=255)                                                                                                                                        gcTTCCCTGTTCGTTGTATTCTGGCGTACCAAATTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGTCAAGGCCGTTATAGACCGACTGAGCCGTGCGGTTGGGAGTATTGGCTTGATACCACAGAGTAGCTGCATTCTTGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGccc                                                                                                                                                          <  2:377076/300‑1 (MQ=255)                                                                                                                                        gcTTCCCTGTTCGTTGTATTCGGGCGTACCAAATTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGTTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGccc                                                                                                                                                          <  2:376850/300‑1 (MQ=255)                                                                                                                                        gcTTCCCGGTTCGTTGTATTCTGGCGTACCAAATTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGccc                                                                                                                                                          <  2:376425/300‑1 (MQ=255)                                                                                                                                        gcTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGcc                                                                                                                                                           <  2:414398/299‑1 (MQ=255)                                                                                                                                        gcTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGGTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAg                                                                                                                                                             <  2:169033/297‑1 (MQ=255)                                                                                                                                        gcTTCCCGGTTCGTTGTATTCGGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCTGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGccc                                                                                                                                                          <  2:377066/300‑1 (MQ=255)                                                                                                                                        gcTTCCCGGTGCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCGTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAgac                                                                                                                                                           <  2:414508/299‑3 (MQ=255)                                                                                                                                               ggTTCGTTGTATTCTGGCGTACCAAAGGTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGcc                                                                                                                                                      <  2:251621/297‑1 (MQ=255)                                                                                                                                                  tCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGTCGACATCATCCGTGCCCGACCGCCCATCAGCGGGCCTGTTACCTGTAGCCCGGCCACCGTc                                                                                                                                                <  2:157098/300‑1 (MQ=255)                                                                                                                                                    gTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGTGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCAt                                                                                                                                              >  2:10768/1‑300 (MQ=255)                                                                                                                                                    gTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCAt                                                                                                                                              >  1:68529/1‑300 (MQ=255)                                                                                                                                                    gTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCAACAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCAt                                                                                                                                              >  1:69199/1‑300 (MQ=255)                                                                                                                                                       gTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACCTCATCCGTGCCGGACCGCACCTCAGCGGGCCTGGTACCTGTAGCCTGGCCACCGTCAtt                                                                                                                                             >  2:698051/1‑298 (MQ=255)                                                                                                                                                                  tACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCt                                                                                                                                 >  2:648518/1‑299 (MQ=255)                                                                                                                                                                  tACCAAAATTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGTCCCATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATTATCCGTGCCGGACCGCCCATCAGAGGGCCTGCTACCTGTAGCCCGGCCACCGTCATCGAGACAGTTTc                                                                                                                                  >  2:648528/1‑298 (MQ=255)                                                                                                                                                                       aaGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGTCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAgg                                                                                                                           <  1:108483/300‑1 (MQ=255)                                                                                                                                                                                   cGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGCCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTaa                                                                                                               >  2:368432/1‑300 (MQ=255)                                                                                                                                                                                         tCCTGGATAATGACCGGAAAATCATCCACACCATAATGTTTGGGGATATGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTTAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGc                                                                                                         <  2:340889/300‑1 (MQ=255)                                                                                                                                                                                                tAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGGCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACAt                                                                                                  <  1:69178/300‑1 (MQ=255)                                                                                                                                                                                                tAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTTAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACAt                                                                                                  <  1:362767/300‑1 (MQ=255)                                                                                                                                                                                                tAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACAt                                                                                                  <  1:363582/300‑1 (MQ=255)                                                                                                                                                                                                tAATGACCGGAAAATCATCCACACCATAATGGTGGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAGGCTTAACAt                                                                                                  <  1:360306/300‑1 (MQ=255)                                                                                                                                                                                                                          tAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATGCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATGGGCGTGATACCACAGAGGAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTc                                                                        <  2:322998/300‑1 (MQ=255)                                                                                                                                                                                                                                    ggATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGt                                                              <  1:382216/300‑1 (MQ=255)                                                                                                                                                                                                                                          gCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTTAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGAc                                                        <  1:45586/300‑1 (MQ=255)                                                                                                                                                                                                                                          gCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGAc                                                        <  1:45640/300‑1 (MQ=255)                                                                                                                                                                                                                                          gCAGCGACTTGCTGACTTCACCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGAc                                                        <  2:93472/300‑1 (MQ=255)                                                                                                                                                                                                                                                  ttGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGAt                                                 <  1:208296/299‑1 (MQ=255)                                                                                                                                                                                                                                                                              ttCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCg                    <  1:226219/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATNATCCGTGCCGGACCGCCCATCAGCGGGCCTGGNACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCAc  >  2:534146/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      ACCATCGTTCATCTGTAGTTGATAGCGACGAGAGTTCGACGCGTTCAGCAGTCGCAAGCGCACCCAGCCACGCGAGACTTCAACGTACGGGCTTTGTACACCGTTAACCAGCAGCGTATCACCAACAAAGCCGCCGCTTCCCGGTTCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCAC  >  NC_000913/3161923‑3162509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑