BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,956,003	G→A	D225N (GAT→AAT)	ilvA →	threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,956,003	0	G	A	97.0%	667.9 / NA	206	D225N (GAT→AAT)	ilvA	threonine deaminase
Reads supporting (aligned to +/- strand): ref base G (3/0); major base A (84/111); minor base C (6/0); total (95/111)
Fisher's exact test for biased strand distribution p-value = 7.33e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

TCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCG  >  NC_000913/3955758‑3956249

     cTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATAAAAGGGATCGCCGTAGAAAAGGAAAACTCCCCCCGCCTGAAAGCAGCGCTGAATGCGGGtgc                                                                                                                                                                                                                                               >  2:1242363/1‑249 (MQ=255)

     cTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACCGATGCCGCAAATCAAAAGGATCGCCGTAAAAGCGGGAGACTACCCCTGCCCTAAAACAGCGCCGGATGCggngcc                                                                                                                                                                                                                                               >  2:1154432/1‑247 (MQ=255)

          acaGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGGGCTGATCAAACAAATGAAGCCGCAAATCAAAGAGATCGCCGTAGAAGCGGAAGAATCCGCCCGCCTGAAAACAGCGCTGAATGCGGGtgaaccgg                                                                                                                                                                                                                                          >  1:1217968/1‑244 (MQ=255)

           cagcagcagGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCCGCCTGAAAGCAGAGCTGAATGCGGGGCATCCggg                                                                                                                                                                                                                                         >  2:674652/1‑250 (MQ=255)

               agcagGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGAACAAACAACTGATGCCGCAAATCAAAGTGATCGGCGGAGAAGCGGAAGACTCCGCCCGCCTGAAAGCAGCGCTGCATGCGGGGCCTCCGGtttat                                                                                                                                                                                                                                     >  2:1097328/1‑248 (MQ=255)

                gcagGGGTGCACCTGGGTGCCGCCGTTCGACCAGCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGTGTCATCCGGTTGATc                                                                                                                                                                                                                                    <  2:109841/251‑1 (MQ=255)

                 cagGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCt                                                                                                                                                                                                                                   <  1:674652/251‑1 (MQ=255)

                  agGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAAAAACTGATGCCGCAAATCAAAGGGATCGACGTAGAAGCGGAAAACTCCACCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGGTGGTCTg                                                                                                                                                                                                                                  >  1:189041/1‑251 (MQ=255)

                  agGGGTTCACCTCGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCACGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAAATGATGCCGCACATCCAAGGGATCGCCGTAGAAGCGGGCGACTCCGCCCGCCTGCAAGCAGCGCTGCATGCGGGGCATCCgggtggt                                                                                                                                                                                                                                     >  2:596891/1‑243 (MQ=255)

                  agGGGTGCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGAt                                                                                                                                                                                                                                     <  1:596891/248‑1 (MQ=255)

                      gTTCACCTGGGTGCCGCCGTTCGCCCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGGCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGGGATCGCCGTAGAAGCGGAAGACGCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCcgc                                                                                                                                                                                                                              <  1:728795/251‑1 (MQ=255)

                        tCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCcgcg                                                                                                                                                                                                                             <  2:1217968/250‑1 (MQ=255)

                         cACCTGGGTGCAGCCGTTCGACCATCCGATGGTGATTGACGGGCAAGGCACGCAGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGATCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGCCCTGGTCCAACAACTTATGGCCCAACTCAACGTGATCGCCGTAGAAGCGGAAGACTCAGCCTGGCCGAAAGCAGCGCTGCATGCGGGTCATCCGGGTGATTTGCcgggcg                                                                                                                                                                                                                           >  1:170068/1‑251 (MQ=255)

                              gggTGCCGCCGTTCGACCATCCGATGTTGATTGCCGGTCAAGGCACGCTGGCTCTGAAACTGCACCAGCAGGACGCCCATCTCGACCTCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGTCGTGGCGGTGCTGATTAAACAACTGATTCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGGCTCCGCCGGCCTGAAAGCGGCGCTGAATGCGTGTCATCCGGTTTATCTGCggcgcggag                                                                                                                                                                                                                        <  2:735254/249‑4 (MQ=255)

                              gggTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCACTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAg                                                                                                                                                                                                                        <  1:841326/249‑1 (MQ=255)

                                        gTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGGTGATCCAACAACAGGTGCCGCAAATCAAAATGATCGCCGTAGAAGCGGAAGACACCGCCTGCCTGAAAGGAGCGCTGAATGGGGGGCAGCCCGTTGGTCTGCCCCGCGTAGGGATATTT‑GCTg                                                                                                                                                                                                           >  2:516003/1‑251 (MQ=255)

                                        gTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGGTCAAACAACTGATGCCGCAAATCCAAGTGATAGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGGGGGGCATCCGGTTGATCTGCCCCGCGGTGGGCTATTT‑TCTg                                                                                                                                                                                                           >  2:556418/1‑251 (MQ=255)

                                        gTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCCTCCGGTTGATCTGCCGCGGGTAGGGCTGTTT‑TCTg                                                                                                                                                                                                           >  1:1113311/1‑251 (MQ=255)

                                        gTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGtt                                                                                                                                                                                                                                        <  1:55798/223‑1 (MQ=255)

                                        gTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGcc                                                                                                                                                                                                                                <  2:797443/231‑1 (MQ=255)

                                        gTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTa                                                                                                                                                                                                                         <  1:117891/238‑1 (MQ=255)

                                        gTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCCGGTCAAACAACTGATGCCGCAAATCAAAGAGATCGCCGTAGAAGCGGAAGGCTCCGCCTGCCTGAAAACAGCGCTGAATGCGGGTCATCCGGGTGGTCTGCcgcgcggaagaacggaagagg                                                                                                                                                                                                            >  2:117891/1‑236 (MQ=255)

                                              ccATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCg                                                                                                                                                                                                     <  2:645736/251‑1 (MQ=255)

                                                aTCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGTCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTa                                                                                                                                                                                                   <  1:516003/251‑1 (MQ=255)

                                                                   gCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCAACTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTg                                                                                                                                                                                <  1:401912/251‑1 (MQ=255)

                                                                    cAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCCAACAACTGATGCCGCAAATCAAAGGGATCGCCGTAGAAGCGGAAGACCCCCCCTTCCTGAAAGCAACGCCGTATGCGGGTCATCCGGGTGATCTGCCCCGCGTATGGCTATTT‑GCTGTAGTGGAAGCGGGAAAAAGCATCGGTgg                                                                                                                                                                               >  2:909247/1‑250 (MQ=255)

                                                                     aaGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGAc                                                                                                                                                                              <  1:10649/251‑1 (MQ=255)

                                                                      aGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACg                                                                                                                                                                             <  1:571264/251‑1 (MQ=255)

                                                                           cgctggcgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAATCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAAcc                                                                                                                                                                        <  1:1242363/251‑1 (MQ=255)

                                                                             ctggcgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGGCGCAAAACAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCtt                                                                                                                                                                      >  2:397133/1‑251 (MQ=255)

                                                                               ggcgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTcc                                                                                                                                                                    <  1:239584/251‑1 (MQ=255)

                                                                               ggcgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTcc                                                                                                                                                                    <  1:361359/251‑1 (MQ=255)

                                                                                gcgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCg                                                                                                                                                                   <  1:539903/251‑1 (MQ=255)

                                                                                 cgctggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTTATCAAACAACTGCTGCCGCAAATCAAAGGGATCGCCGTAGAAGCGGAAGACTCCGCCCGCCTGAAAGCCACGCTGAATGCGGGTCATCCGGTTGGTCTGCCGCGCGTAGGGGTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGt                                                                                                                                                                  >  1:1160175/1‑251 (MQ=255)

                                                                                    tggAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGGTCAAACAACTGATGCCGCAAATCAAAGAGATCGCCGTAGAAGCGGGAGACTCCCCCTGCCTGGAAGAAGCGCTGAATGCGGGTCATTCGGGTTATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTGGCGGTAAAACGCCTCGGTGACGAAACCTTCCGtttt                                                                                                                                                               >  1:461353/1‑250 (MQ=255)

                                                                                    tggAACTGCTCCAGCAGGACGCCCAGCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTaaa                                                                                                                                                                                           <  1:1025050/223‑1 (MQ=255)

                                                                                          tGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGGGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGCATGCGGGTCATCCCGGTGGTCTTCCGCGCGGAGGGCTATTT‑GCTGAAGGCGTAGCGGGAAAACGCAACGGTGACGAAACCTTCCGGTTATGcc                                                                                                                                                           >  2:242105/1‑249 (MQ=255)

                                                                                             tCCAGCAGGACGCCCCTCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAAACTTCCGTTTATGCCAgg                                                                                                                                                        >  1:1104583/1‑249 (MQ=255)

                                                                                                   agGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAAAAACTGATGCCGCACATCAAAGTGGTCGCCGTAGAAGAGGGAGACTCCCCCTGCCTGAAAGCAGCGCTGCATGCGGGACATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGGAAAACGCATCGGTGACGAAAACTTCCGTTTATGCCAGGAGTAtctc                                                                                                                                                >  1:1130387/1‑251 (MQ=255)

                                                                                                    ggACGCCCATCTCGACCGGGTATTTGGGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGGTGAGCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTGCCGTTTATGCCAGGAGTATCTcg                                                                                                                                               <  2:423720/251‑1 (MQ=255)

                                                                                                     gACGCCCATCTCGGCCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTcga                                                                                                                                              <  2:189041/251‑1 (MQ=255)

                                                                                                     gACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTcga                                                                                                                                              <  1:220725/251‑1 (MQ=255)

                                                                                                        gCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGGAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGGTTATGCCCGGAGTATCTcgacgg                                                                                                                                           >  2:453500/1‑250 (MQ=255)

                                                                                                          ccATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGAGTGGCTGCTGGCGTGGAGGTACTGATTAAAGAAGTGGAGCCGCAAATCAAAGTAGTCGCACTACAGGCGGAAGACAACCCCTCCCTGAAAGCAGCGCGGAATGAGGGGCATCCGGCTGATCTGCCGGGCGTAGGGGTATTTTGC‑GAAGGCGGGGCGGGAAAACGAATCGGTGACGAGACCCTCCGATTATGTCAGGAggctttcgacg                                                                                                                                            >  1:1027718/1‑238 (MQ=255)

                                                                                                                  aCCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGTCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCAcc                                                                                                                                 <  1:1186457/251‑1 (MQ=255)

                                                                                                                    cgcgTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCGGATTAAACAACTGATGCCGCAAATAAAAGTGATCGCCGTAGGAGAGGGAGACCCCGCCTGCCTTAAAGCAGCGCTGAAAGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCCGGGAAGAAACCTTCCGTTTATGGCAGGGGGATCCcgacgaacacatcaccct                                                                                                                               >  2:40250/1‑239 (MQ=255)

                                                                                                                      ggTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCg                                                                                                                             <  1:622571/250‑1 (MQ=255)

                                                                                                                           ttGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAg                                                                                                                         <  1:773723/250‑1 (MQ=255)

                                                                                                                               gCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgcgatg                                                                                                                    <  2:598452/251‑1 (MQ=255)

                                                                                                                                ccANTCGGCTGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgcgatgc                                                                                                                   <  2:1130387/251‑1 (MQ=255)

                                                                                                                                  aGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAgcgatgcga                                                                                                                 <  2:1050933/251‑1 (MQ=255)

                                                                                                                                     cggcggcggcggTCTGGCTGCTGGCGTGGCGGTGATGCACCAACAACTGATGCCCCAAATCAAAGGGATCGCCGTAGAAGCGGAAGAATCCCCCTGCCTGAAAGCAGCGCCGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTTGAGGCGTAGCGGTAAAACGCATCGGGGACGAAACCTTCCCTTTATGCCAGGGGGATCTCGACCACATCATCACCCTCGATAGCGGTGCGGTCt                                                                                                              >  1:1006010/1‑251 (MQ=255)

                                                                                                                                      ggcggcggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATa                                                                                                                          <  2:360028/238‑1 (MQ=255)

                                                                                                                                         gtcggcggTCTGGCTGCTAGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGGGATCGCCGTAGATGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCCTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCGTGCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTCTgc                                                                                                          <  2:1027718/249‑1 (MQ=255)

                                                                                                                                         ggcggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGGGATCGCCGTAGAAGCGGAAGACTCCGCCCGCCTGAAAGCAGCGCTGAATGCGGGGCATCCGGGTGATCTGCCGCGCGGAGGGGTATTT‑GTTGAAGGCGTAGCGGTAAAACGCATCGGGGACGAAACCTTCCGTTTATTCCCGGGGTATCTCGGCGACATCATCACCGTCGATAGCGATGCGGTCTGTgc                                                                                                          >  2:75724/1‑251 (MQ=255)

                                                                                                                                            ggcggTCTGGCTGCTGGCGTGGCGGTGCTGATTAAACAACTGATGCCGCAAATCAAAGTGATCGGCGTAGAAGCGGGAGACTCCGCCCGCCTGAAAACAGCGCCGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCCGGGACGAAACCTTCCGTTTATGCCAGGAGGATCTCGGCGACCTCATCACCGTCGATAGCGATGCGGTCTGTgcggc                                                                                                       >  1:1031412/1‑251 (MQ=255)

                                                                                                                                            ggcggTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCCTCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGGAGGGCTATTT‑GCTGAAGGCGTAGCGGTCACACGCATCGGTGACGAAACCCTTCGTTTATGCCAGGAGGATCTCGACGCCATCATCACCGTCGATAGCGATGCGATCTGTgcggg                                                                                                       >  2:312058/1‑250 (MQ=255)

                                                                                                                                                           ggcgtggcgGTGCTGATCAAACAACTGATGCCGCAAATCACAATGATCGCCGTAAAAGCGGAAGACTCCGCCTGCCTGCAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCCCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGAAGAAACCTTCCGTTTTTGCCAGGGGTATCTCGCCGAACTCATCACCCTCGATTGCGATGCGATCTGTGCGGCGGTGAAGGATTTAtt                                                                                        >  2:564858/1‑251 (MQ=255)

                                                                                                                                                            gcgtggcgGTGATGATCAAACCACTGATGCCGCAAATCAAAGAGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGCATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGTTCTGTGCGGCGATGAAGGATTTATTc                                                                                       >  2:155507/1‑251 (MQ=255)

                                                                                                                                                              gtggcgGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGa                                                                                     <  1:10507/251‑1 (MQ=255)

                                                                                                                                                                ggcgGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGa                                                                                     >  1:480751/1‑249 (MQ=255)

                                                                                                                                                                         gATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTgcgcg                                                                          <  2:834555/251‑1 (MQ=255)

                                                                                                                                                                            cAAACCACTGATGCCGCAAATCAACGTGATCGCCGTCGACGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCgg                                                                       >  2:713856/1‑251 (MQ=255)

                                                                                                                                                                             aaaCAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTgcg                                                                                                         <  2:463004/216‑1 (MQ=255)

                                                                                                                                                                             aaaCAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGt                                                                      <  1:257202/251‑1 (MQ=255)

                                                                                                                                                                              aacaacTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTg                                                                     >  1:1071183/1‑251 (MQ=255)

                                                                                                                                                                              aacaacTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTg                                                                     <  1:397133/251‑1 (MQ=255)

                                                                                                                                                                               acaacTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCtgt                                                                                                            <  1:1092475/211‑1 (MQ=255)

                                                                                                                                                                               acaacTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTGTTCGAAGATGTGCGCGCGGTgg                                                                    <  1:139470/251‑1 (MQ=255)

                                                                                                                                                                                 aacTGAGGCCGCAAATCAAAGTGATCGCCGGAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCg                                                                  <  2:290522/251‑1 (MQ=255)

                                                                                                                                                                                     gATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGGCGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGa                                                                <  2:1031412/249‑1 (MQ=255)

                                                                                                                                                                                         ccGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTcgac                                                                                                                                             <  1:411477/168‑1 (MQ=255)

                                                                                                                                                                                             aaaTCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGgc                                                      >  1:962345/1‑251 (MQ=255)

                                                                                                                                                                                              aaTCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTGTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGgcg                                                     <  2:1104583/251‑1 (MQ=255)

                                                                                                                                                                                                tCAAAGTGATCGCCGTCGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGgcgcg                                                   >  1:418398/1‑251 (MQ=255)

                                                                                                                                                                                                   aaGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCg                                                                                                                                <  2:325473/171‑1 (MQ=255)

                                                                                                                                                                                                   aaGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCg                                                                                                                                >  1:325473/1‑171 (MQ=255)

                                                                                                                                                                                                        aTCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctggcgct                                           >  2:870287/1‑251 (MQ=255)

                                                                                                                                                                                                        aTCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgctggcgct                                           <  1:890905/251‑1 (MQ=255)

                                                                                                                                                                                                          cGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGgc                                                      >  2:1065442/1‑238 (MQ=255)

                                                                                                                                                                                                          cGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGGATCTCGACTACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGcgctggcgctgg                                         <  1:713856/251‑1 (MQ=255)

                                                                                                                                                                                                              gTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCgcgct                                                 <  2:1192616/239‑1 (MQ=255)

                                                                                                                                                                                                               tAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCATCTGGCGCGCTGGCGCTGGCgggg                                    >  2:781269/1‑250 (MQ=255)

                                                                                                                                                                                                                aGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCgacgac                                                                                                                                          >  1:793917/1‑148 (MQ=255)

                                                                                                                                                                                                                aGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGaa                                   <  1:801951/251‑1 (MQ=255)

                                                                                                                                                                                                                 gAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAAt                                  <  1:682981/251‑1 (MQ=255)

                                                                                                                                                                                                                 gAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAAt                                  >  2:623183/1‑251 (MQ=255)

                                                                                                                                                                                                                 gAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAAt                                  <  1:980933/251‑1 (MQ=255)

                                                                                                                                                                                                                    gCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGa                                >  1:308050/1‑250 (MQ=255)

                                                                                                                                                                                                                     gggAAGACTCCGCCGGGCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATGT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACAGCATCACCGTCGATAGCGATGCGATCTGTGGGGGGATGAAGGATTTATTCGAAGATGTGCGTGCGGTGGAGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGaaa                              <  1:1193250/250‑1 (MQ=255)

                                                                                                                                                                                                                      ggAAGACTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGaaaa                             <  2:82627/251‑1 (MQ=255)

                                                                                                                                                                                                                           aCTCCGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAatat                        <  2:1006010/251‑1 (MQ=255)

                                                                                                                                                                                                                           aCTCCGCCTGACAGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGAAGGGCTATTT‑GATGAAGGCGTAGCGGTAAAACGCATCGGCGACGAACCCTTCCGGTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGAAAGCGATGAGATCTGAGCGGCGATGCAGGATTTATTCGAAGATGTGCGCGAGGTGGCGGGACGCTCTGGCGCGCTGGCGCTGGCGGCAATGCAAAAattt                        >  2:896684/1‑249 (MQ=255)

                                                                                                                                                                                                                              ccGCCTGCCTGAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATc                     >  1:349265/1‑251 (MQ=255)

                                                                                                                                                                                                                               cgagctcctgAAAGCAGCGCTGAATGCGGGTCAGCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTTGCGCTGGCGGGAATGAAAAAATATATCg                    <  2:864696/245‑1 (MQ=255)

                                                                                                                                                                                                                                     cctgAAAGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGGATGAAAAAATATATCGcgatcg              >  1:864696/1‑246 (MQ=255)

                                                                                                                                                                                                                                        aaaaGTAGCGCTGAATGCGGGTCATCCGTTTGATCTGCCGCGAGTAGTGCTTTTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGAGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTGTTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGTCGCTGGCGGGCATGAAAAAAAATATCGCCCTGcac            >  2:178148/2‑250 (MQ=255)

                                                                                                                                                                                                                                         aaaGCAGCGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCCGcaca           >  2:495075/1‑250 (MQ=255)

                                                                                                                                                                                                                                              agcGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTc     <  1:155507/251‑1 (MQ=255)

                                                                                                                                                                                                                                              agcGCTGAATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTc     <  2:128265/251‑1 (MQ=255)

TCGAACTGTCACAGCAGCAGGGGTTCACCTGGGTGCCGCCGTTCGACCATCCGATGGTGATTGCCGGGCAAGGCACGCTGGCGCTGGAACTGCTCCAGCAGGACGCCCATCTCGACCGCGTATTTGTGCCAGTCGGCGGCGGCGGTCTGGCTGCTGGCGTGGCGGTGCTGATCAAACAACTGATGCCGCAAATCAAAGTGATCGCCGTAGAAGCGGAAGACTCCGCCTGCCTGAAAGCAGCGCTGGATGCGGGTCATCCGGTTGATCTGCCGCGCGTAGGGCTATTT‑GCTGAAGGCGTAGCGGTAAAACGCATCGGTGACGAAACCTTCCGTTTATGCCAGGAGTATCTCGACGACATCATCACCGTCGATAGCGATGCGATCTGTGCGGCGATGAAGGATTTATTCGAAGATGTGCGCGCGGTGGCGGAACCCTCTGGCGCGCTGGCGCTGGCGGGAATGAAAAAATATATCGCCCTGCACAACATTCGCG  >  NC_000913/3955758‑3956249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`