Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,871,153	T→G	N51T (AAC→ACC)	truD ←	tRNA pseudouridine(13) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,871,153	0	T	G	100.0%	395.0 / NA	99	N51T (AAC→ACC)	truD	tRNA pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (49/50);  total (49/50)

GTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCACG  >  NC_000913/2870910‑2871397                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     gTAAAGGCTTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTGGTGCCGCGCATACTCCAGCCCCTGGCAGCCTTCCAGTTGAAAGGCGCCC‑AGATCGGGCATTTCCTGG‑CCCGG‑CACGCGAGCGCATACCCACTGTTCCGTAATAGCTTGTTTGTTTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGATTTTT‑CAGGAATTTCGCA‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCTGTTTTGAg                                                                                                                                                                                                                                               <  1:265857/251‑1 (MQ=255) gTAAAGGCGTTCCCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCCCCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATACCCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAg                                                                                                                                                                                                                                               <  1:897936/251‑1 (MQ=255)   aaatgCGTTCCCTTTCAGCGCGCCTAAACGCAGCTTGCGTTTGGGCCCCCCATACTCCAGCCCCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCCCTGTCCCGTAACAGAATTTTTGTTTTTTTGCCC‑AGCGAAG‑CTAACTTCACGGGCTTAATTTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGa                                                                                                                                                                                                                                             <  2:80371/247‑1 (MQ=255)   aaaGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAgg                                                                                                                                                                                                                                              >  1:1188308/1‑250 (MQ=255)     gggCGTTACCTTCCAGCGCGCCAAAACGCAGCTTGCGTTTGTGCCGCGCATCCCCCAGCCCCGGGCAGCCTTCCAGTTGAAAGGCGCTA‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATACCCCCTGTCCCGTAACAGCATTTTTGTTTTTTTGCCC‑AGCGAAG‑CGGACTTCAGGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTCC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGAtt                                                                                                                                                                                                                                           <  2:1167651/250‑1 (MQ=255)     aggctttcccTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATCCTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGAATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGATTTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGAtt                                                                                                                                                                                                                                           <  1:115311/243‑1 (MQ=255)      ggCGTTACCTTTAAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGTCAGCCTTCCAGTTGAAAGTCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCAATGTTCCGTAACAGCATGTTTGTCTTTTTGCGC‑AGCGAAG‑CTGGCTTCACGGGCATGAATTTT‑CAGGAATTCCGCC‑AGTGC‑ATCCGCCACAAAACGGTCATTGCAGCCGGGTTTGAGGATTc                                                                                                                                                                                                                                          >  2:1036353/1‑251 (MQ=255)       gctttACCTTCCAGCGCGCCAAAACGCAGCTTGCGCTTGGCCCGCGCATCCTCCAGCCCCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGTCATTCCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATTTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGATTTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCt                                                                                                                                                                                                                                         <  2:826083/248‑1 (MQ=255)        cGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCt                                                                                                                                                                                                                                         >  1:78775/1‑250 (MQ=255)          ttcccTTTCAGCGCGCCCAAACGCAGCTTGCGCTTGTGCCGCGAATACGCCAACACCTGCCGGCCTTCCATTTGAAAGGCGCTC‑AGATCGGGCATTTC‑TTGTCCCGG‑CACGCGCGCGCATACCCACTGTTCCGTAACAGCATGTTTTTCTTTTTGCCC‑AGCGAAG‑CTACCTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTaa                                                                                                                                                                                                                                       <  2:1091401/247‑1 (MQ=255)            cccTTTCAGCGCCCAAAAAGGCATCTTGCGTTTGTGCCGCGCATCCCCCAGCCCCGGGCAGCCTTCCAGTTGAAGGGCTCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGCGCGCATAACCCCTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGATTTTT‑CAGGAATTTCGCC‑ATTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAAcc                                                                                                                                                                                                                                     <  2:1188308/249‑1 (MQ=255)               tttCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGAGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGa                                                                                                                                                                                                                                  >  1:830908/1‑250 (MQ=255)                  cAGCGCGCCTAAGCGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAatat                                                                                                                                                                                                                              >  2:1179968/1‑251 (MQ=255)                   ggcgcgcCTAACCGCAGTTTGCGTTTGGGCCGCGCATACTCCAGCCCCGGGCAGCCTCCCAGTTAAAAGGCGCTC‑AGATCGGGCATTCCCTTG‑CCGGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATTTTTGTCTTTTTGCCC‑AGCAAAG‑CTGACTTCCCGGGCATGATTTTT‑CAGGATTTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATg                                                                                                                                                                                                                             <  2:809446/250‑1 (MQ=255)                   aGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCACCCTCCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGC‑CACGCGAGCGCATAACCACTGTCCCGTACCAGCATGTTTGTCTTTTGGCCC‑AGCGAAG‑CTGACTTCACGGGCAGGAATTTT‑CAGGAATTCCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATg                                                                                                                                                                                                                             <  1:420687/251‑1 (MQ=255)                    gcgcccCTAAACGCAGCTTGCGTTTGGGCCGCGCATCCCCCAGCCCCTGGCAGCCTTCCAGTGGAAGGGCGCTC‑AGATCGGGCATTTCCTGG‑CCCGG‑CACGCGGGCGCATAACCACTTTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGTATTTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGc                                                                                                                                                                                                                            <  2:275463/251‑1 (MQ=255)                      ccgcCTAAACGCAGCTTGCGTTTGTGCCGCGCATCCCCCAGCCCCGGGCAGCCTTCCATTGGAAAGGCGCTC‑AGATCGGGCATTCCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATTTTTGTTTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGATTTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTc                                                                                                                                                                                                                          <  2:438491/250‑1 (MQ=255)                        gcCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCAc                                                                                                                                                                                                                        >  1:1212199/1‑251 (MQ=255)                            aagcGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCCCCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCtt                                                                                                                                                                                                                     <  1:1179968/247‑1 (MQ=255)                                gCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCAcc                                                                                                                                                                                                                 >  1:1131527/1‑250 (MQ=255)                                 cAGCTTGCGCTTGGGCCGCGCATACTCCAGCCCCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCAt                                                                                                                                                                                                               <  1:999469/251‑1 (MQ=255)                                    cTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATc                                                                                                                                                                                                              >  1:858596/1‑249 (MQ=255)                                                    gcATCCTCCAGCCCCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑ATATCGGGCTTTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCGGTAACAGCATGTTTTTTTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTAC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATc                                                                                                                                                                                            <  2:1065283/251‑1 (MQ=255)                                                    gcATACTCCAGCCCCTGGAAGCTTCCCAGTTAAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑GGGGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGATTTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATc                                                                                                                                                                                            <  2:757775/251‑1 (MQ=255)                                                        cccgcgctcttccgatctcTTCCAGTTGAAAGGCCCTC‑AGTTCGGGCTTTTCCTTG‑CCCGG‑CCCGCGCGCGCATACCCCCTTTCCCGTAAAAGCTTGTTTGTTTTTTTGCCC‑AGCGAAC‑CTGACTTCACGGGCATAATTTTT‑CAGGAATTTCGCC‑AGTCC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTcc                                                                                                                                                                                        <  2:218719/233‑1 (MQ=255)                                                           ccagcgcctggccggtttCCAGTTGAAAGGCG‑TCTAGATGGGGCATTTCCTTG‑CCCGC‑CCCACGAAGACATAACCGCTGTTCCGTAGCATCGTGTTTGTCTTTTTGCCC‑AGGGAAC‑CGGTCTTCACGGCTATGAATTTT‑CAGGAATTAGCCC‑AGTGT‑GTCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTcca                                                                                                                                                                                       <  1:446457/233‑1 (MQ=255)                                                            cAGCACCTGGCAGCCTTCCAGTTGAAAGGCGTTC‑AAATCGGGCATTTCCTAG‑CCCGG‑CACGCGAGCGCATACCCCTTGTCCCTTACCAGCATGTTTGTCTTTTGCCCC‑AGCAAAG‑CTGCTTTCAGGGGCATGAATTTT‑CAGGAATTTCGCC‑ATTGC‑TTCCGCCATAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCt                                                                                                                                                                                           <  1:166258/244‑1 (MQ=255)                                                            cAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCATAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCt                                                                                                                                                                                           >  2:166258/1‑244 (MQ=255)                                                              cccccggacAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCTAAACAGCATTTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTccaccacc                                                                                                                                                                                  <  1:1118060/243‑1 (MQ=255)                                                                      cacccTTCCAGTTGAAAGGCCCTC‑AGACCGGGCTTTTCCTTG‑CCCGG‑CCCGCGAGCGCATACCCACTTTTCCGTAACAGCATTTTTGTCTTTTTCCCC‑AGCGAAG‑CTGACTTCACGGTCATGATTTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTc                                                                                                                                                                          <  1:910037/248‑1 (MQ=255)                                                                       cgCCTCCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATACCCACTGTTCCTTAACAGCTTTTTTGTCTTTTTCCCC‑AGCGAAG‑CTGCCTTCAGGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCt                                                                                                                                                                         <  2:865935/250‑1 (MQ=255)                                                                         ccTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTc                                                                                                                                                                       >  1:523395/1‑251 (MQ=255)                                                                          cTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTcc                                                                                                                                                                                        >  1:218719/1‑233 (MQ=255)                                                                             ccAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTCTTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGCCTTCCgg                                                                                                                                                                    >  1:1230987/1‑250 (MQ=255)                                                                             ccAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCgg                                                                                                                                                                    >  1:93551/1‑250 (MQ=255)                                                                                   gAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAata                                                                                                                                                                                                                               >  1:597696/1‑185 (MQ=255)                                                                                       ggCGCTA‑AGAGCGGGCATTTCGTTG‑CCCGG‑CACTGGAGCTAAAAACCGCTGTCCCGAAACAGCATGTTTGTCTTTTGCCCC‑GGCGACG‑CTAACTTCACGGGCAT‑AATTTTGCAGGAATTTCGCC‑AGTGC‑AACCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTca                                                                                                                                                         <  2:890563/251‑1 (MQ=255)                                                                                         cgcTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTcagc                                                                                                                                                       >  1:34611/1‑251 (MQ=255)                                                                                         cgcTA‑AGATCGGGCATTTCCTGC‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCAAAG‑CTGACTTCACGGGCATTAATTTT‑CAGGAATTTCCCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTcagc                                                                                                                                                       <  2:1212199/251‑1 (MQ=255)                                                                                          gcTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTcagc                                                                                                                                                       >  2:514191/1‑250 (MQ=255)                                                                                          gcTC‑AGAGCGGGCATTTCCTTC‑CCCGG‑AACGCGAGCGCATAGCCACTTTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTcagca                                                                                                                                                      <  1:812372/251‑1 (MQ=255)                                                                                            cc‑AGATCGGGCATTTCCTTG‑CCGGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCTTGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGATTTTT‑CAGTAATTCCGCC‑GGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCagcagc                                                                                                                                                    <  1:182648/250‑1 (MQ=255)                                                                                               aGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGCTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGccc                                                                                                                                                  >  2:557037/1‑251 (MQ=255)                                                                                                        cattCCTTT‑CCCGGGCACGCGAGCGTATAACCACTGTTCTTTAAAAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGTCTTCCCGGGAAGGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCTCAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCtt                                                                                                                                          <  1:1095039/249‑1 (MQ=255)                                                                                                                        cACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGa                                                                                                                           <  1:1024310/251‑1 (MQ=255)                                                                                                                         aCGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTTTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCaaa                                                                                                                                                                                              <  1:294602/183‑1 (MQ=255)                                                                                                                         aCGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCaaa                                                                                                                                                                                              >  2:294602/1‑183 (MQ=255)                                                                                                                           ccgaccgcATACCCCCTTTTCGGTAGCAGCATGTTTGTTTTTCGGCCC‑AGCGAGG‑CTGGCTCCTGGGGCATGAATTGT‑CAAGAGTGCCTCC‑AG‑GCTATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCTCAAAGGCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGt                                                                                                                        <  1:1036353/246‑1 (MQ=255)                                                                                                                             gAGCGCATACCCCCTCTTCCGTAGTACCATGTTTGT‑TTTTTGCCCCAGGGAAC‑CTGACTTCTCGGCCTTGAATTTT‑CAGTATTTTCGCC‑AGTGC‑TTCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTTGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTaa                                                                                                                      <  1:585101/251‑1 (MQ=255)                                                                                                                                cgcATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTg                                                                                                                   >  2:958687/1‑251 (MQ=255)                                                                                                                                cgcATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACAACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTg                                                                                                                   >  2:628359/1‑251 (MQ=255)                                                                                                                                  cATAACCACTGTTCCGTAACAGCATGTTTTTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTg                                                                                                                   <  1:902047/249‑1 (MQ=255)                                                                                                                                  cATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTg                                                                                                                   >  2:902047/1‑249 (MQ=255)                                                                                                                                  ataccccactttcccGTAACAGCATTTTTGTTTTTTTCCCC‑AGCGAAG‑CTGACTTCACGGGCATAAATTTT‑CAGGAATTTCGCC‑AGTCC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCCCAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTgag                                                                                                                 <  2:719280/238‑1 (MQ=255)                                                                                                                                   cccgatctttttCCGAAACAGCATGTTTGTTTTTTTGCCC‑AGCGAAG‑CTGCCTTCACGGGCATGATTTTT‑CAG‑AATTTCCCCCAGGCC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTgag                                                                                                                 <  2:391111/240‑1 (MQ=255)                                                                                                                                           tGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTgag                                                                                                                 >  1:391111/1‑242 (MQ=255)                                                                                                                                           tGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCaa                                                                                                         >  1:429130/1‑250 (MQ=255)                                                                                                                                                cGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCa                                                                                                    >  1:694644/1‑250 (MQ=255)                                                                                                                                                 gTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAAt                                                                                                  >  2:755847/1‑251 (MQ=255)                                                                                                                                                 gTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAAt                                                                                                  >  1:482196/1‑251 (MQ=255)                                                                                                                                                  tAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATc                                                                                                 >  1:675134/1‑251 (MQ=255)                                                                                                                                                   aaCAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATc                                                                                                 >  2:715039/1‑250 (MQ=255)                                                                                                                                                     aagaggttttttCTTTTTGCCA‑AG‑GAAGGCTGACTTAACGGGGAAGAATTTA‑AAGAAATTTGGCC‑AGGGC‑AACCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCAtt                                                                                              <  2:93551/240‑1 (MQ=255)                                                                                                                                                           ttttGTCTTTTTCCCC‑AGCGAAG‑CTGACTTCCCGGGCATGAATTTT‑CAGGATTTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttgtt                                                                                         <  1:586683/249‑1 (MQ=255)                                                                                                                                                             ttGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGcc                                                                                      >  2:1086296/1‑251 (MQ=255)                                                                                                                                                                tttttttGCCC‑CGCGAGC‑CTTACTCCACGGGAATGATTTTT‑CAGGATTTTCCCC‑ATTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCtt                                                                                    <  2:988361/248‑1 (MQ=255)                                                                                                                                                                  tttttGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAAt                                                                                 >  2:357385/1‑251 (MQ=255)                                                                                                                                                                   ttttGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATg                                                                                >  2:929683/1‑251 (MQ=255)                                                                                                                                                                       gCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGCATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAACCTCAATCATTTTGTTGCCTTAATGAGt                                                                             >  2:1198908/1‑250 (MQ=255)                                                                                                                                                                       gCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTCCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGt                                                                             <  1:357385/250‑1 (MQ=255)                                                                                                                                                                         cc‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAgc                                                                          >  1:984460/1‑251 (MQ=255)                                                                                                                                                                         cc‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAgc                                                                          >  1:290395/1‑251 (MQ=255)                                                                                                                                                                            aGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAgcgc                                                                        >  2:1164030/1‑251 (MQ=255)                                                                                                                                                                             gCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGcc                                                                       >  1:803937/1‑251 (MQ=255)                                                                                                                                                                               gAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCAc                                                                     >  1:1000198/1‑251 (MQ=255)                                                                                                                                                                                  gtgtGACTTCCAGGGCTTGATTTTT‑CAGAAATTTGCCC‑ATTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTAGCAAACTCAATCATTTTGTTGCCTAAATGAGTAGCGCCACCg                                                                   <  2:1230987/248‑1 (MQ=255)                                                                                                                                                                                     tGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCtt                                                                >  1:857930/1‑251 (MQ=255)                                                                                                                                                                                     tGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCtt                                                                >  2:619327/1‑251 (MQ=255)                                                                                                                                                                                       ccTTCACGGGCATGATTTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTca                                                              <  2:523395/250‑1 (MQ=255)                                                                                                                                                                                           cACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAg                                                           <  1:480328/250‑1 (MQ=255)                                                                                                                                                                                            aCGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGc                                                         >  2:76224/1‑251 (MQ=255)                                                                                                                                                                                                gCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATc                                                     >  1:250230/1‑251 (MQ=255)                                                                                                                                                                                                          ttcgaaactttCCCC‑AGTCC‑ACCCGCCACAAAACGGGTATTGCATCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCTTCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGTATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCCCCGCTTCACAGGCAATCCCTTcccc                                             <  2:285549/242‑1 (MQ=255)                                                                                                                                                                                                                  tttCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCAACTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCggg                                    >  2:233508/1‑250 (MQ=255)                                                                                                                                                                                                                   gtCGCC‑TGTGA‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGt                                    <  2:858596/249‑1 (MQ=255)                                                                                                                                                                                                                      gCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCACATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTACCAAACTCAATCATTTTGTTGCCTTAATGa                                                                               >  1:1116775/1‑204 (MQ=255)                                                                                                                                                                                                                        cgaGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATc                               <  2:429130/249‑1 (MQ=255)                                                                                                                                                                                                                        c‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATcc                              >  2:826536/1‑251 (MQ=255)                                                                                                                                                                                                                         aaGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATcc                              <  2:34611/250‑1 (MQ=255)                                                                                                                                                                                                                                   cGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACATGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTc                     <  1:958687/251‑1 (MQ=255)                                                                                                                                                                                                                                   cGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTc                     <  2:857930/251‑1 (MQ=255)                                                                                                                                                                                                                                    gCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGTTAAATCCCAGTTTTTcc                    >  2:151191/1‑251 (MQ=255)                                                                                                                                                                                                                                       acaAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCg                   >  1:598238/1‑249 (MQ=255)                                                                                                                                                                                                                                       acaAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCg                   <  2:598238/249‑1 (MQ=255)                                                                                                                                                                                                                                           aaCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGcc                                                                       >  1:255002/1‑193 (MQ=255)                                                                                                                                                                                                                                           aaCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGcc                                                                       <  2:255002/193‑1 (MQ=255)                                                                                                                                                                                                                                              gggTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgt                  <  1:1173358/243‑1 (MQ=255)                                                                                                                                                                                                                                              gggTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgt                  >  2:1173358/1‑243 (MQ=255)                                                                                                                                                                                                                                                      cAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCACg  >  2:1218037/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     GTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTC‑AGATCGGGCATTTCCTTG‑CCCGG‑CACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCC‑AGCGAAG‑CTGACTTCACGGGCATGAATTTT‑CAGGAATTTCGCC‑AGTGC‑ATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCACG  >  NC_000913/2870910‑2871397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑