Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3630C.88.9% 439.4 / 46.1 117intergenic (‑490/+919)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/13);  new base . (72/32);  total (72/45)
Fisher's exact test for biased strand distribution p-value = 1.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009132,173,3640C.88.9% 441.2 / 44.2 117intergenic (‑491/+918)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/13);  new base . (72/32);  total (72/45)
Fisher's exact test for biased strand distribution p-value = 1.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGC  >  NC_000913/2173121‑2173614
                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                          
cAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCAACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGt                                                                                                                                                                                                                                                   >  2:163711/1‑251 (MQ=255)
 ggCATGAGCCGCCAGTGGGGTGTGCAAACCAATAGTTTGCGACCGGATCCACAGGGTGAGGCTCATAATGATGACCCCGGAGATTAAGGTGCGGAACATATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACTTGATTCCCCGGCACACAAACAG‑CAATTAAAATGGGG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTc                                                                                                                                                                                                                                                  <  2:190641/250‑1 (MQ=255)
  gCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTcc                                                                                                                                                                                                                                                 >  1:244598/1‑251 (MQ=255)
   cATTACCCGCCGGTTGGGTGTGCAAACCAATAGTTGGCGTCGCGATCCAAAGGGTGATGCTCATAATGATGACCCCCGAGATTAAGGTGCGGAAAAGATTTCCACGATGCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTccc                                                                                                                                                                                                                                                <  2:210132/251‑1 (MQ=255)
    aTTAGCCGCCAGTTGGGTGAGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTccc                                                                                                                                                                                                                                                >  1:1100200/1‑250 (MQ=255)
            ccAGTTGGGGGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAAc                                                                                                                                                                                                                                       <  1:990263/251‑1 (MQ=255)
                  gggTGGGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGGGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCGGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGa                                                                                                                                                                                                                                 <  2:828527/251‑1 (MQ=255)
                          aaaCCAATAGTTTGGGCCGGGACCCACAGGGGGATGCTCAGAATGAGCACACCCGAGATTAAGGTCCGGAACAGATTCCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGAAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAAGGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGcc                                                                                                                                                                                                                         <  2:1100200/251‑1 (MQ=255)
                           aaCCAATAGTTGGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTCCCACGATGCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAGCGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                                                                                                                                        <  2:127614/251‑1 (MQ=255)
                              cAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATc                                                                                                                                                                                                                     >  2:928835/1‑251 (MQ=255)
                               aaTAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATc                                                                                                                                                                                                                     >  2:317385/1‑250 (MQ=255)
                                 tAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                                                  >  1:131980/1‑251 (MQ=255)
                                        cggcgagATCCACAGGGTGATGCACATAAAGGTAACACCCGAGATTAAGGGGCGAAAGAGATTTCCGGGATGCAGGGAGACCGCCATCGCCACGAAGAAGCCGAGGGTGGCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAGCAG‑CAATAAAAATGGTG‑AGGGGAA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt                                                                                                                                                                                                           <  1:671531/245‑1 (MQ=255)
                                        cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt                                                                                                                                                                                                           >  2:889256/1‑251 (MQ=255)
                                         gTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTg                                                                                                                                                                                                          >  1:178097/1‑251 (MQ=255)
                                         gTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTg                                                                                                                                                                                                          >  1:889230/1‑251 (MQ=255)
                                            gcgATCCACAGGGTGATGCTCATACTGATGACACCCGAGATTAAGACGCGGACCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGACGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTCTGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAcc                                                                                                                                                                                                       >  2:731909/1‑251 (MQ=255)
                                                tccgatgtttGATGTTCATAATGATGCCCCCCGAAATAAAGGTGCGGAACATATTTCCCCGAGGCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc                                                                                                                                                                                                    <  2:752757/241‑1 (MQ=255)
                                                      gggTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTc                                                                                                                                                                                             >  2:752719/1‑251 (MQ=255)
                                                      gggTGAGGCTCAAAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTc                                                                                                                                                                                             <  1:62304/251‑1 (MQ=255)
                                                        gTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc                                                                                                                                                                                                    >  1:752757/1‑242 (MQ=255)
                                                        gTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc                                                                                                                                                                                           >  2:980418/1‑251 (MQ=255)
                                                          gATGCTCATAATGATGACCCCCGAGATTAAGGTGCGGAACAGATTCCCACGATGCACGGCGCCCGCCATCGCCACGAAGAAGCCGAGGGTGGAAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCt                                                                                                                                                                                         <  1:752719/251‑1 (MQ=255)
                                                            tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGAG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                                                                                                                                        >  2:49139/1‑218 (MQ=255)
                                                             gctcataagcaggacccccGACATTAAGGTGCGCCACCGATTCCCACGAGGCACGGCCCCCGCCATCGCCCCGAAAAAGCCGATGGTGGAAAAACCGCCAACCGGCAGCCCCTGATTCCCCGGCACACAAACAG‑CAATTAAACTGGTG‑AGTGGGA‑TAAAAATCAGCCTCGCCG‑‑ACCCCCCCGCCGTATGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTGTa                                                                                                                                                                                      <  2:638860/235‑1 (MQ=255)
                                                                   aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAc                                                                                                                                                                                >  1:276594/1‑251 (MQ=255)
                                                                    atgatgACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                                                                                                                                        >  1:718280/1‑210 (MQ=255)
                                                                      gatgaCCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTa                                                                                                                                                                              <  1:67703/250‑1 (MQ=255)
                                                                      gatgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc                                                                                                                                                                                                             >  1:1207917/1‑219 (MQ=255)
                                                                       atgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATTGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTTCGCCTGTAACCGACTACg                                                                                                                                                                            >  2:1079700/1‑251 (MQ=255)
                                                                          acacCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGCTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                                         >  2:409051/1‑251 (MQ=255)
                                                                             cccGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGcttg                                                                                                                                                                      >  2:731300/1‑248 (MQ=255)
                                                                               cGAGTTTAAGGGGGGGAACAGATTTCCACGATGCACGGCGACCGCCGTCGCCACGAAGAAGCCGATGGGGGAAAGATCGCAAAGCGGCAGCACCTGATTACCCGGCACGCAAACAG‑CAATTAAAA‑GGTGGAGTGGAA‑TAAAAACCAGGCTGGCCGATAC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCt                                                                                                                                                                    <  1:745460/251‑1 (MQ=255)
                                                                               cGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGTCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCt                                                                                                                                                                    >  2:1091011/1‑251 (MQ=255)
                                                                                 agaTTACGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAgcttgctt                                                                                                                                                                   >  2:96568/1‑250 (MQ=255)
                                                                                 agaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGGG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAgcttgctt                                                                                                                                                                   >  1:1011708/1‑250 (MQ=255)
                                                                                     tAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCg                                                                                                                                                               >  2:395914/1‑250 (MQ=255)
                                                                                           gCGGAACAGATTCCCAGGATGCACGGGGACCGCCATCGCCACGAAGAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCg                                                                                                                                                        <  1:539879/251‑1 (MQ=255)
                                                                                             ggaccAGATTCCCACGTTGCACGCCGACCGCCACCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAATTCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGtt                                                                                                                                                      <  1:928835/247‑1 (MQ=255)
                                                                                             ggAACAGATTTCCACGGTGCACGGCGCCCGCCACCGCCACGAAGACGCCGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAG‑AAATTAAAAGGGTG‑AGGGGGA‑TAAAAACCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGtt                                                                                                                                                      <  1:1091011/251‑1 (MQ=255)
                                                                                             ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGtt                                                                                                                                                      >  2:88357/1‑251 (MQ=255)
                                                                                                  aGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAcc                                                                                                                                                 >  2:554120/1‑251 (MQ=255)
                                                                                                  aGATTGCCCCGATGCCCGGCGCCCGCCACCGCCACGAAGAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAG‑CAATAAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAcc                                                                                                                                                 <  2:889230/251‑1 (MQ=255)
                                                                                                           ggATGCAGGGCGCCCCCCATCGCCACAAAGAAGCCGTTGGGGGAAAGATCGCCAAACGGCAGCCCTTGATTCCCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCatgat                                                                                                                                        <  2:131980/250‑1 (MQ=255)
                                                                                                           cGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCatgat                                                                                                                                        <  1:395914/251‑1 (MQ=255)
                                                                                                                   ggCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTa                                                                                                                                 >  1:552849/1‑250 (MQ=255)
                                                                                                                     cGACCGCCACCGCACCGAAGAATCCGAGGGTGGAAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACCCAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAAGCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc                                                                                                                              <  1:588790/251‑1 (MQ=255)
                                                                                                                             aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGc                                                                                                                                                                     >  2:321844/1‑204 (MQ=255)
                                                                                                                             aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑AAATTAAAAGGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGc                                                                                                                                                                     <  1:321844/204‑1 (MQ=255)
                                                                                                                             aTCGCAACGAAGAAGCCGAGGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGGG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTgg                                                                                                                      <  1:1043787/251‑1 (MQ=255)
                                                                                                                               cGCCACGAAAAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCCCACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCa                                                                                                                    <  1:9082/251‑1 (MQ=255)
                                                                                                                               cGCCACAAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATCACCAGGCACAAAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCTAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCa                                                                                                                    <  1:7154/251‑1 (MQ=255)
                                                                                                                                gCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTACTCACCCGTGGCAt                                                                                                                   >  2:1231959/1‑251 (MQ=255)
                                                                                                                                gCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGAAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt                                                                                                                   >  1:1052538/1‑251 (MQ=255)
                                                                                                                                       agaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagcagc                                                                                                            >  2:501641/1‑251 (MQ=255)
                                                                                                                                              aatgggggaaaaaacgcaaaagggCAGCACCTGATTACCGGGCACACAAACAG‑CAATAAAAATGGTG‑AGTGGGA‑AAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTg                                                                                                     <  1:939144/229‑1 (MQ=255)
                                                                                                                                                   gggCAAAATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGAA‑TAAAAAGCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgccgcc                                                                                                <  1:88357/250‑1 (MQ=255)
                                                                                                                                                       aaGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGttt                                                                                            >  2:933070/1‑251 (MQ=255)
                                                                                                                                                            cGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAcc                                                                                       >  1:214378/1‑251 (MQ=255)
                                                                                                                                                                      gaggcaccgaatACCCGGCACACAAAAAGTAAATAAAAAGGGTG‑AGTGGAA‑TAAAAATCGGGTTTGC‑‑AGACCCCTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCGCGGCCGCCGTTTTTACCGCCAGCTGc                                                                              <  2:552849/240‑1 (MQ=255)
                                                                                                                                                                      cggcaCCGGATTCCCCGACACACAAACAG‑CAATAAAAAGGGGG‑AGGGGGA‑TAAAAAGCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCa                                                                             <  1:163711/249‑1 (MQ=255)
                                                                                                                                                                            cTGATTCCCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGAA‑TAAAAATCAGGCTGGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgccg                                                                                                  <  1:273797/224‑1 (MQ=255)
                                                                                                                                                                              gATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCt                                                                     >  2:247651/1‑251 (MQ=255)
                                                                                                                                                                              gATTACCCGGCACAAAAACAG‑CAATTAAAAGGGTG‑AGTGGAA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACAcc                                                                      <  1:409051/250‑1 (MQ=255)
                                                                                                                                                                               ataccccggccaaaaacaaG‑CAATTAAAATGG‑G‑AGGGGGAATAAAAATCAGTCTCGCCCAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAACAAGGACCTCTTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCtt                                                                    <  2:1011708/233‑1 (MQ=255)
                                                                                                                                                                               aTTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCtt                                                                    >  1:485308/1‑251 (MQ=255)
                                                                                                                                                                                 tccccGGCACACAAACAC‑CAATAAAAAGGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCttt                                                                   <  1:96568/248‑1 (MQ=255)
                                                                                                                                                                                      ggCACACAAACAG‑CAATTCAAATGGAG‑TGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCACAGCAGCAACGCCGGATCAAAGCCAATCAGACACACCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAATCATCCAAGATAGGTTTAATCACCCGAGGCATTAGCAGCATCACGACCGCCGTTTTTACCGCCAGCTGCAATACACCTTAGAcct                                                              >  1:1001472/1‑248 (MQ=255)
                                                                                                                                                                                       gCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCg                                                            >  2:990446/1‑251 (MQ=255)
                                                                                                                                                                                        cacacaAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGtt                                                                                                                                                      <  1:1066033/160‑1 (MQ=255)
                                                                                                                                                                                        cacacaAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCg                                                                                      >  1:738/1‑224 (MQ=255)
                                                                                                                                                                                         acacaAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACt                                                                                                      >  1:1242223/1‑207 (MQ=255)
                                                                                                                                                                                           aaaaaCAG‑CAATTAAAATGGTG‑AGGGGGA‑AAAAAACCAGGTTTCCCTAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAc                                                        <  2:1065077/249‑1 (MQ=255)
                                                                                                                                                                                            caAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAcc                                                       >  2:1015563/1‑251 (MQ=255)
                                                                                                                                                                                               aCAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa                                                                                                                                >  1:444705/1‑175 (MQ=255)
                                                                                                                                                                                               aCAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgc                                                    >  2:246272/1‑251 (MQ=255)
                                                                                                                                                                                                  g‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgcg                                                   >  2:583113/1‑249 (MQ=255)
                                                                                                                                                                                                     aaTTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTa                                                                                         >  2:311123/1‑209 (MQ=255)
                                                                                                                                                                                                     aaTTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAgg                                                >  1:982347/1‑250 (MQ=255)
                                                                                                                                                                                                     aaTTAAAATGGTG‑AGTGGGA‑AAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTa                                                                                         <  1:311123/209‑1 (MQ=255)
                                                                                                                                                                                                            aTGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAt                                        >  1:428997/1‑251 (MQ=255)
                                                                                                                                                                                                               gTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAAt                                     >  1:1241988/1‑251 (MQ=255)
                                                                                                                                                                                                                 g‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                                    >  1:684023/1‑250 (MQ=255)
                                                                                                                                                                                                                        ggaaaaaaaccgggCTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGcccc                              <  2:276594/239‑1 (MQ=255)
                                                                                                                                                                                                                             aaaaTCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac                         <  1:881126/251‑1 (MQ=255)
                                                                                                                                                                                                                               aaTCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac                         >  2:881126/1‑249 (MQ=255)
                                                                                                                                                                                                                                aTCAGGCTGGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaa                      <  1:246272/251‑1 (MQ=255)
                                                                                                                                                                                                                                     gCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAcccc                 >  2:499019/1‑250 (MQ=255)
                                                                                                                                                                                                                                     gCTTGCCGAT‑CC‑CCCCGCCGTATTTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCa                 <  2:125683/251‑1 (MQ=255)
                                                                                                                                                                                                                                       ttGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATc                           >  1:928090/1‑239 (MQ=255)
                                                                                                                                                                                                                                         ttgccaaccccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATc                           <  2:928090/227‑1 (MQ=255)
                                                                                                                                                                                                                                          tgccgacacccccGCCGTATGCCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACg               <  2:428997/241‑1 (MQ=255)
                                                                                                                                                                                                                                          ggccgacccccccGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAACCCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACgg              <  1:1231959/242‑1 (MQ=255)
                                                                                                                                                                                                                                           gccgttaccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGt             <  1:731300/241‑1 (MQ=255)
                                                                                                                                                                                                                                           cGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTg            >  2:1031887/1‑250 (MQ=255)
                                                                                                                                                                                                                                              gataccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCAAGCTGCAATACACCTTGGACACCGTAACCCGCGGGGATGCCGATAATCAGCCCCATCAAAAAAcccccgtgga           >  1:475755/8‑243 (MQ=255)
                                                                                                                                                                                                                                              aatcccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc          <  1:216119/244‑1 (MQ=255)
                                                                                                                                                                                                                                                 accaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAgg       >  2:217093/5‑251 (MQ=255)
                                                                                                                                                                                                                                                   ccccGCCGTATGTCACAGCAGACACGCCGGATCAAGGCAAAGCAGGACCTCCGGACCGCCGAACTTTGCCGGTAAACGACTACTAGCTTTCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTGAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCTATAATCAGCCCCATCACAAAACCCACGGTGACAGGCt     <  2:695916/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      cgccgTATGTCCCAGCAGCCACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCAGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGc  >  2:533530/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                          
CAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAG‑CAATTAAAATGGTG‑AGTGGGA‑TAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGC  >  NC_000913/2173121‑2173614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑