Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	820,914	IS5 (+) +4 bp	coding (122‑125/714 nt)	ybhM →	Bax1‑I family protein YbhM

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	273955 =	NA (NA)	118 (1.290)	71/590	0.1	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	820914 =	0 (0.000)					coding (122/714 nt)	ybhM	Bax1‑I family protein YbhM
*	?	NC_000913	= 275149	NA (NA)	80 (0.880)	48/588	0.6	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 820917	0 (0.000)					coding (125/714 nt)	ybhM	Bax1‑I family protein YbhM

TATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACCCCTTGTATCTGGCTTTCACGAAGCCGAACTGTCGCTTGATGATGCGAAATGGG  >  NC_000913/273952‑274251    |                                                                                                                                                                                                                                                                                                         gaTGGAAGGTGGGAATAAGCGGGGAAATTCTTCTCTGCCGACTCATTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAGCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAATAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACCCCTTGTATCTGGCTTTCACGAAGCCGAACGGTCGCTTGATGATGCGAAATggg  <  2:68538/299‑1 (MQ=255)    |                                                                                                                                                                                                                                                                                                         TATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACCCCTTGTATCTGGCTTTCACGAAGCCGAACTGTCGCTTGATGATGCGAAATGGG  >  NC_000913/273952‑274251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.