Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,385	4 bp→CAA	coding (530‑533/1656 nt)	opgD →	glucans biosynthesis protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,385	0	T	C	100.0%	157.4 / NA	43	F177S (TTT→TCT)	opgD	glucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/24);  total (19/24)
*	NC_000913	1,497,386	0	T	.	100.0%	200.2 / NA	43	coding (531/1656 nt)	opgD	glucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/24);  total (19/24)
*	NC_000913	1,497,387	0	C	A	100.0%	137.7 / NA	43	L178N (CTC→AAC)	opgD	glucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/24);  total (19/24)
*	NC_000913	1,497,388	0	T	A	100.0%	143.7 / NA	43	L178N (CTC→AAC)	opgD	glucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/24);  total (19/24)

GTATTCAATACGACGCCGAAAAATCGCTCTGGCATAACGTTGAGAACCGTCAACTGGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTGATTATGGATGTGGAAAATCACCTGTATGCGCGCAAAGACATTAAAC  >  NC_000913/1497103‑1497666                                                                                                                                                                                                                                                                                           |||                                                                                                                                                                                                                                                                                        gTATTCAATACGACGCCGAAAAATCGCTCTGGCATAACGTTGAGAACCGTCAACTGGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGTGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTAttt                                                                                                                                                                                                                                                                         >  2:336358/1‑300 (MQ=255)                        tCGCTCTGGCATAACGTTGAGAACCGTCAACTGGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATc                                                                                                                                                                                                                                                  <  1:465120/300‑1 (MQ=255)                               ggCATAACGTTGAGAACCGTCAACTGGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATa                                                                                                                                                                                                                                              >  1:195082/1‑297 (MQ=255)                               ggCATAACGTTGAGAACCGTCAACTGGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACAAATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCAGTGATGTAGTATCATC‑AACGGCGCGGGTTATTTCCGCGCCGTTGATGATACATATCAAt                                                                                                                                                                                                                                               >  2:274116/1‑296 (MQ=255)                                       gTTGAGAACCGTCAACTGGATGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGTGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCCGGTGTTGCTACACAACAATTAGAAGGGCAAAGTGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCAAACTGGCGCGCCGTGCTGTAGTATCATC‑AACGGTGCGAGTGCTTTCCGCGCCGTTGCTGATACGTGTCACTACGGTTTGt                                                                                                                                                                                                                                      >  2:70954/1‑297 (MQ=255)                                       gTTGAGAACCGTCAACTGGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGt                                                                                                                                                                                                                                      >  2:191597/1‑297 (MQ=255)                                       gTTGAGAACCGTCAACTGAACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGCTGTAGTATCATC‑AACGGCGCGCGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTc                                                                                                                                                                                                                                     >  2:71207/1‑298 (MQ=255)                                                              tCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGacac                                                                                                                                                                                                             <  1:527258/299‑1 (MQ=255)                                                                 gTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AANGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTac                                                                                                                                                                                                         >  1:516296/1‑300 (MQ=255)                                                                 gTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGTCCCGCGGCCTGGCGATCGACACTTac                                                                                                                                                                                                         >  1:168714/1‑300 (MQ=255)                                                                   tcttcCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTacac                                                                                                                                                                                                       <  1:80524/300‑1 (MQ=255)                                                                     ttcCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCg                                                                                                                                                                                                     <  1:221924/300‑1 (MQ=255)                                                                      tcCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCTTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCg                                                                                                                                                                                                     <  1:221935/299‑1 (MQ=255)                                                                             gggaatgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCCACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCCAATACAACGATGCAGGTGTTGATACAAAACAACTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGAGCGAGTTATTTCTGCGCCGTTGATGATACATATCAATACAGTTTGTCGGCACGCGGCCTGGCGATCGACACTTACAccaccagta                                                                                                                                                                                               >  2:418698/1‑291 (MQ=255)                                                                               gaatgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAaaga                                                                                                                                                                                           >  2:628630/1‑300 (MQ=255)                                                                                                                   tAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATANAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATAc                                                                                                                                                       <  1:522420/300‑1 (MQ=255)                                                                                                                           gCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAAc                                                                                                                                               <  1:418698/300‑1 (MQ=255)                                                                                                                             aaCACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCTTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTGTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAAcc                                                                                                                                              <  2:598787/299‑1 (MQ=255)                                                                                                                                                        tCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCg                                                                                                                  <  1:275966/300‑1 (MQ=255)                                                                                                                                                          gCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCTTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGt                                                                                                                 <  1:201636/299‑1 (MQ=255)                                                                                                                                                               gAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAACCCCCGAACTGGCGCGCCGTGCTGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGTGATCGACACTTACACCGACAGTAAAGAAGAGtt                                                                                                                                                                                     >  2:190642/1‑226 (MQ=255)                                                                                                                                                                 gttgttCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGAt                                                                                                         <  1:551213/300‑1 (MQ=255)                                                                                                                                                                 ggtgttCAAATACAACGATGCAGGTGTTGATACAAAGCAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGAt                                                                                                         <  2:252829/298‑1 (MQ=255)                                                                                                                                                                   tgttCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAg                                                                                                       <  1:426204/300‑1 (MQ=255)                                                                                                                                                                             cAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTACGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCACGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTACAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCAtt                                                                                             >  2:295109/1‑300 (MQ=255)                                                                                                                                                                                 gATGCAGGTGTTGATACAAAACAATTAGAAGTGCAAAGCGATCTCGTCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTg                                                                                         <  2:154106/300‑1 (MQ=255)                                                                                                                                                                                   tGCAGGTGTTGATACAAAATGATTAGAAGTGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCAAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGGCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTAcgg                                                                                         <  2:71290/298‑3 (MQ=255)                                                                                                                                                                                       ggTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACCGGCGCGCCGTGATGTAGTATCATC‑AACGACGCGAGTTATTTCCGCGCCGCTGATGATACATATCAATACGGTTTGTCGGCCCACGGCCTGGCGATCGACACCTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCATTCTGGTTTGATACGGTAAAAACGGGGGCAACTACCTTTCCCGTTTATGTGTTGCTCGCTAGCGCAAGCATTACTGGt                                                                                       >  2:102224/1‑296 (MQ=255)                                                                                                                                                                                            tGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCTTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAg                                                                              <  1:455729/300‑1 (MQ=255)                                                                                                                                                                                              aTACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTGAAGCCCCCGAACTGTCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGtt                                                                            <  2:684705/300‑1 (MQ=255)                                                                                                                                                                                              aTACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTTGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGTGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGtt                                                                            <  2:372887/300‑1 (MQ=255)                                                                                                                                                                                                   aaaCAATTAGAAGTGCAAAGCGATCTCGTCTGTGCCGATTTTCGCGTGTTTAAAGCCCCCGAACTGACGCGCCGTGATGTAGGATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGATTTTTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCACCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAACGCCAGCATTAcctc                                                                                        <  2:293847/283‑4 (MQ=255)                                                                                                                                                                                                      cAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGACCTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGAAACTTACACCGACAGTAAAGAAGAGTTCCACGACTTTAACGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTAACGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTAt                                                                      >  2:369375/1‑298 (MQ=255)                                                                                                                                                                                                       aaTTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATcc                                                                    <  1:71207/299‑1 (MQ=255)                                                                                                                                                                                                       aaTTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTGAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATcc                                                                    <  1:70954/299‑1 (MQ=255)                                                                                                                                                                                                                                  ttGCCGATTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTTAGAAAAGTCAGGTGATTATGGa                                        <  2:63564/300‑1 (MQ=255)                                                                                                                                                                                                                                                  gtTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTGATTATGGATGTGGAAAATCACCt                         >  1:98257/1‑299 (MQ=255)                                                                                                                                                                                                                                                   tttAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTAACGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTGATTATGGATGTGGAAAATCAc                           >  1:97955/1‑296 (MQ=255)                                                                                                                                                                                                                                                          cccccGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTNCCCGACTTTCCCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTAACGTTTATGCGTTGCTCGATAGCGCCAGCACTACTGGTGCCTATAAGTTCACTATCTATTGGGAGAAAAGTCAGGTGATTATGGATGTGGAACATCAACTGTATgcgc                 >  2:504348/1‑299 (MQ=255)                                                                                                                                                                                                                                                           ccccGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTGATTATGGATGTGGAAAATCACCTGTATgcgcgc               >  1:682035/1‑300 (MQ=255)                                                                                                                                                                                                                                                                aaCTGGCGCGCCGTGANGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTGANTATGGATGTGGAAAATCANCTGTATGCGCGCAAAGa          >  1:600797/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 aCTGGCGCTCTGTGATGTAGTATCATC‑AACTGCACGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTTGCGATCGACACTTACACCGACAGTAAATAGGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTGATTATGGATGTGGAAAATCACCTGTATGCGCGCAAAGAc         <  2:210739/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gcCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATTAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTGATTATGGATGTGGAAAATCACCTGTATGCGCGCAAAGACATTAAAc  <  2:40970/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                           |||                                                                                                                                                                                                                                                                                        GTATTCAATACGACGCCGAAAAATCGCTCTGGCATAACGTTGAGAACCGTCAACTGGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGGTGATTATGGATGTGGAAAATCACCTGTATGCGCGCAAAGACATTAAAC  >  NC_000913/1497103‑1497666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑