Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,123,823	2 bp→AG	coding (231‑232/648 nt)	grxB ←	reduced glutaredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,123,823	0	G	A	100.0%	163.6 / NA	48	L78L (CTG→TTG)	grxB	reduced glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/26);  total (22/26)
*	NC_000913	1,123,824	0	T	G	100.0%	178.4 / NA	48	L77F (TTA→TTC)	grxB	reduced glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/26);  total (22/26)

GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGAGTAT  >  NC_000913/1123535‑1124113                                                                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                                                                  gAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCCGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTAAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGc                                                                                                                                                                                                                                                                                         >  2:361046/1‑300 (MQ=255)    tatCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCACACGTAAATCATCGCTGATATTCGTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCATTGGAACGTTTGCCGGTCAAGAACGGTTTGCCGt                                                                                                                                                                                                                                                                                      <  2:348958/300‑1 (MQ=255)            tCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAATTTa                                                                                                                                                                                                                                                                              >  2:43581/1‑300 (MQ=255)                          aTTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAAc                                                                                                                                                                                                                                                                <  2:689607/300‑1 (MQ=255)                          aTTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAAc                                                                                                                                                                                                                                                                <  1:535342/300‑1 (MQ=255)                              aCGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCNGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGAt                                                                                                                                                                                                                                                             <  2:551304/299‑1 (MQ=255)                                        gTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACCCATCAAATGCCGATTTGGCAAAACGCGGCACTAGCAGTTTGTTGGCGTCGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCttt                                                                                                                                                                                                                                                  >  1:702962/1‑300 (MQ=255)                                            gACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCt                                                                                                                                                                                                                                                >  1:452885/1‑298 (MQ=255)                                            gACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTAAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTAGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCt                                                                                                                                                                                                                                                >  2:409215/1‑298 (MQ=255)                                                                   gTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAGTATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCttttt                                                                                                                                                                                                                       >  1:443148/1‑300 (MQ=255)                                                                             gCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAAt                                                                                                                                                                                                                >  1:527917/1‑297 (MQ=255)                                                                                gATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAAc                                                                                                                                                                                                          >  1:627369/1‑300 (MQ=255)                                                                                    ttcgtAATCAGACCGTCAGAGGGGGCCAGCAGGTCGGCAAAATGACCCGCGCTGGACTCTTTCTTGGCGACGAAGNATGTGCGCGCGGCGGGATTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTGGGCGTAGCCATTGACCGTGCGCAGCCACTCTTCAATGGCAGTGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAAc                                                                                                                                                                                                                                                                <  2:220622/238‑1 (MQ=255)                                                                                        tAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCCTCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCCTGATTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGttttt                                                                                                                                                                                                  >  2:279941/1‑300 (MQ=255)                                                                                                ccGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGTGGCAACAGTAGTTTGTTGGCGTAGCCATTGGCCTTGCGCAGCCACTCTTCAATGGCAGGGGAAAGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTgcccgacc                                                                                                                                                                                          >  2:66832/1‑300 (MQ=255)                                                                                                ccGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTgaccgacc                                                                                                                                                                                          >  1:366072/1‑300 (MQ=255)                                                                                                                                    gcgcTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAg                                                                                                                                                       <  1:638880/299‑1 (MQ=255)                                                                                                                                      gcTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAAc                                                                                                                                                    <  2:690563/300‑1 (MQ=255)                                                                                                                                         ggCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTAAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAATAGAACAt                                                                                                                                                  >  2:283581/1‑299 (MQ=255)                                                                                                                                             tctttcttGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTNGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTa                                                                                                                                               <  2:549520/298‑1 (MQ=255)                                                                                                                                                      tCGACGAAATATTTGCGCGCGGCGGGAGGAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACTGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATATCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGAc                                                                                                                                        <  2:52311/296‑1 (MQ=255)                                                                                                                                                                          ggcggGAGTAGAAAACTCATCAAATGCCGATTCGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTTGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAAt                                                                                                                <  1:263687/300‑1 (MQ=255)                                                                                                                                                                                 gTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGc                                                                                                           >  1:123629/1‑298 (MQ=255)                                                                                                                                                                                      aaaCTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTAAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACCTGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCttt                                                                                                    >  2:280451/1‑300 (MQ=255)                                                                                                                                                                                          tcatcaAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCNATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAg                                                                                                 <  1:157845/299‑1 (MQ=255)                                                                                                                                                                                          tcatcaAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTGGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCNATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAg                                                                                                 <  1:158765/299‑1 (MQ=255)                                                                                                                                                                                            atcaAGTGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTTAACGATGTCCATGCTTTCTGTCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTCGACCGACCATCCGGGTGGGTGTTTCTGCTTCGTCGTTGAGCAAAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGTCttttatcc                                                                                               <  2:703768/299‑6 (MQ=255)                                                                                                                                                                                                      gATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGc                                                                                      <  2:594167/298‑1 (MQ=255)                                                                                                                                                                                                      gATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGNCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGc                                                                                      <  2:553619/298‑1 (MQ=255)                                                                                                                                                                                                         tgggCAAAACGCGACAACAGCAGTTGTTTGGCGTAGCCATTGACCGTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGa                                                                                 <  2:43370/298‑1 (MQ=255)                                                                                                                                                                                                                        aaCAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTTAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGc                                                                  <  1:681199/300‑1 (MQ=255)                                                                                                                                                                                                                        aaCAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGc                                                                  <  1:487052/300‑1 (MQ=255)                                                                                                                                                                                                                           agcaaTTTGTTGGCGTAGCCATTGACCTTCCGCAGCCACTCTTCAATGTCAGTGGAACGTTTGCCGGTCAAGAATGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATAGAGCGGCTTTCATCTTTTTGCAGAATTGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGTGGATATTTTTCATGCCGAAAATCATGCGTGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCtt                                                                <  2:390689/299‑1 (MQ=255)                                                                                                                                                                                                                               gTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGt                                                           >  2:640083/1‑300 (MQ=255)                                                                                                                                                                                                                                 ttgttgGCGTAGCCATTGACCTTGCGCAGCCACTCTTAAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGt                                                           >  1:107329/1‑298 (MQ=255)                                                                                                                                                                                                                                      ggCGTAGCCATTGACCTTTCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTCTCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGAatcct                                                    >  1:226281/1‑300 (MQ=255)                                                                                                                                                                                                                                            gCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGTGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCAttt                                              <  1:55058/300‑1 (MQ=255)                                                                                                                                                                                                                                            gCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCAttt                                              >  2:500535/1‑300 (MQ=255)                                                                                                                                                                                                                                            gCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCAttt                                              <  1:460158/300‑1 (MQ=255)                                                                                                                                                                                                                                            gCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGGTTGCCGTCGAGTTTAGCGACATAGTGAACGATGTCCATGCTTTCTTGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCAttt                                              <  2:288745/300‑1 (MQ=255)                                                                                                                                                                                                                                                       tGCGCAGCCACTCTTCAATGGGAGTGGAACATTTGCCGGGCAAGAACGGTTTGCCGTCGAGTTTATCGACATATTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTTTCATCTTTTTGCAGAATTGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTTAGGCAGTAAGGGCATTGATCGTAAATGTATAGCTTCACGTGACTCCTCCAttt                                              <  2:84981/289‑1 (MQ=255)                                                                                                                                                                                                                                                                 ctctTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGt                         >  1:475438/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   gttCAATGGCAGGGGAACGTTCGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGGTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAGTGGGAACTTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAACATCATGCGGGCTTTGAGGCAGTAATGGCAGTGATCGTAAATGTATAGCTTAACGTGACTCATCCATTTGGCTGTCAGTTCACTTCCAGTAt                       >  2:708001/2‑300 (MQ=255)                                                                                                                                                                                                                                                                   ctTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTAt                       >  1:651198/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   ctTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCAAGTGACTCCTACATTTGGCTGTCAGTTCACTTTCCGTAt                       >  2:628982/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        attgCAGGGGAACGTTGGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAgg                  <  2:72884/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                        tGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGTGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGAGTAt  <  1:361473/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        tGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGAGTAt  <  1:361046/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                                                                  GAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGAGTAT  >  NC_000913/1123535‑1124113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑