Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,353,360	C→T	H238H (CAC→CAT)	glpA →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,353,360	0	C	T	100.0%	153.7 / NA	43	H238H (CAC→CAT)	glpA	anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/18);  total (25/18)

AGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGC  >  NC_000913/2353072‑2353649                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                  aGTTCCGGATGGCACCGTTGATCCATTTCGGCTGACCGCAGCAAACATGCTGGATGCCAAAAAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAAcc                                                                                                                                                                                                                                                                                         <  2:379252/299‑1 (MQ=255)  gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCa                                                                                                                                                                                                                                                                                        >  1:173003/1‑299 (MQ=255)          tgacACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGGAGTCACTGGGCTGATTCGTGAAGGCGCGACGGTGTGTGGTGTTCGTGTACGTAACCATCGCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCGGATCATGGATCATCGCATTAACCAGCATGTGAt                                                                                                                                                                                                                                                                               <  2:206067/297‑1 (MQ=255)            gCACCGTTGATCCATTNCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGCTCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGNTGATCATGGATCATCGCANTAACCAGCATGTGATCa                                                                                                                                                                                                                                                                             >  1:600579/1‑300 (MQ=255)                                            aaCATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTAAACGGCGAAACTCAGACCCTTCATACACCTGTCGTGGTTAATGCCGCTGGGATCTAGGGGCCACACATTGCCGAATATGCCGATCTGCGCATTCGCATNTTCCCGGCGAAAGGATCGCTGCTGATTATGGATCATCGCATTAACCAGCCTGTGATCAACCGCTGACGTACACCTTCCGACGCCGATAtt                                                                                                                                                                                                                                             >  2:558821/1‑300 (MQ=255)                                                                                 ttACCGCTCATGAAGTCACGTGGCTGATTCTTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCTGCGAAACTCATGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGGGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTac                                                                                                                                                                                                          <  1:279166/298‑1 (MQ=255)                                                                                 ttACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATNGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTacca                                                                                                                                                                                                        >  2:562656/1‑300 (MQ=255)                                                                                            gAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGGACGTAACCATCTCACCGGCGAAACTCATNCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTTGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGt                                                                                                                                                                                             <  1:511377/300‑1 (MQ=255)                                                                                                         tGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACg                                                                                                                                                                                <  1:629385/300‑1 (MQ=255)                                                                                                                            ggTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCAGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGAAGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCAACTCTTTCCGTATTGATTACAACGAGATTGAAGATAATCGAGt                                                                                                                                                             >  2:357058/1‑300 (MQ=255)                                                                                                                              tgtgCGGTGTTCGTGTACTTAACCACCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGTGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGTTTCCTGTCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAg                                                                                                                                                              <  2:163642/297‑1 (MQ=255)                                                                                                                              tgtgCGGTGTTCGTGTACGTAACCATCTAACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACCTTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGTATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTTATTACAACGAGATTGCCTACAATCGAGTGa                                                                                                                                                           >  2:395479/1‑300 (MQ=255)                                                                                                                              tgtgCGGTGTTCGTGTACGTAACCATCTAACCGGCGAAACTCAGGCCCTTAATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAAACGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACAAGATTGACGATAATCGAGTGa                                                                                                                                                           >  2:392097/1‑300 (MQ=255)                                                                                                                               gtgCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGAc                                                                                                                                                          >  1:613919/1‑300 (MQ=255)                                                                                                                                    gtgtTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGNATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCa                                                                                                                                                      >  2:515376/1‑299 (MQ=255)                                                                                                                                    gtgtTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCAGGCGAAAGGATCGCTGCTGATCATGGATCATCGAATTAACCAGCATGTGATCAACCGATGCAGTAAACCTTCCGAAGCCGATATTCTGGTGCCTTGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTCTTGATTACAAAGAGATTGACGATAATCGAGTGACGGCa                                                                                                                                                      >  2:64420/1‑299 (MQ=255)                                                                                                                                       ttCGTGTACGTAACCATCTCACCGGCGAAACTCATGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCagaag                                                                                                                                                  <  1:554494/300‑1 (MQ=255)                                                                                                                                       ttCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCagaag                                                                                                                                                  <  1:168842/300‑1 (MQ=255)                                                                                                                                                   aCCATCTCACCTGCGAAACTCAGGCCCTTCATGCACCTTTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCATAAGAGGTTGATATTc                                                                                                                                      <  2:101232/300‑1 (MQ=255)                                                                                                                                                      aTCTCACCGTCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGTGATCGGTGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGTTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCTACGCCGATATTCTGGTGCCTGGCGATACCATTTTGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCATAAGAGGTTGATATTctgc                                                                                                                                   <  2:694178/300‑1 (MQ=255)                                                                                                                                                           aCCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGTGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCTGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACACCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGt                                                                                                                              <  1:568094/300‑1 (MQ=255)                                                                                                                                                                        aGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAAc                                                                                                                 <  1:450638/300‑1 (MQ=255)                                                                                                                                                                             cTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGcc                                                                                                            >  1:420696/1‑300 (MQ=255)                                                                                                                                                                             cTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTATTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGcc                                                                                                            >  1:20699/1‑300 (MQ=255)                                                                                                                                                                             cTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCAGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGcc                                                                                                            >  2:291405/1‑300 (MQ=255)                                                                                                                                                                              ttCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGccc                                                                                                           >  1:480627/1‑300 (MQ=255)                                                                                                                                                                              ttCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAAACAGCATGTGATCAACCGCTGCAGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGccc                                                                                                           >  2:161419/1‑300 (MQ=255)                                                                                                                                                                                         gTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGGTTGACGATAATCGAGTGACGGCAGAGGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCACGTGATGGc                                                                                                 >  2:99600/1‑299 (MQ=255)                                                                                                                                                                                         gTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGc                                                                                                 >  1:109786/1‑299 (MQ=255)                                                                                                                                                                                                           gggATCTGGGGGCAACACGTTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCAGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATCTTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGCTTACAACGCGCTTGACGATAATCGAGTGGCGGCAGAAGGGGTTGATATTCTGCTGCGTGGAGGGGAAAAACTGGCACACGTGATGGCGAAAACGAGCAGTTTGc                                                                                >  2:100510/1‑298 (MQ=255)                                                                                                                                                                                                           gggATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAGGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTCCGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCCGAAGAGGTTGATATTTTGCTGCGTGACGGGGAAAAACTGGCACCCGTGATAGCGAAAACACGCATTTTGc                                                                                >  2:100246/1‑298 (MQ=255)                                                                                                                                                                                                               tCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAAATGGCACCCGTGATTGCGAAAACGCGCATTTTGCGGGCCt                                                                          >  2:240935/1‑300 (MQ=255)                                                                                                                                                                                                                  gggggCAACGCATTGAAGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCANCCGCTGCCGTAAACCTTCCGACGCCGCTATTCNGGTGCCTGGCGATACCATTTAGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGCCGGCAGACGGGGTTGATATTCTGCTGCGTGGAGGGGAAAAACTGGCCCCCGTGATGGCGCAACCGCGCATTTTGCGGGCCTAt                                                                        >  2:532342/1‑299 (MQ=255)                                                                                                                                                                                                                  gggggCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCANCCGCTGCCGTAAACCTTCCGACGCCGATATTCNGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCATAtt                                                                       >  2:532692/1‑300 (MQ=255)                                                                                                                                                                                                                        aaCACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCg                                                                 <  1:556714/300‑1 (MQ=255)                                                                                                                                                                                                                                             aTCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGc                                             >  1:482434/1‑299 (MQ=255)                                                                                                                                                                                                                                                      ttCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGAcc                                   <  1:433811/300‑1 (MQ=255)                                                                                                                                                                                                                                                            tGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCACATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCTACGCCGATATTCTGGTGCCTGTCGATACCATTTCGCTGATTGGTACTACCTCNTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGGGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCg                             <  2:238205/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   ggCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGANGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACg                         <  1:153304/297‑1 (MQ=255)                                                                                                                                                                                                                                                                      gAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACg                    <  1:407951/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATNTTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGACTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGt    >  2:239002/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAAAGTCAGCCGCGGCATCGt    >  2:285089/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       tCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGtgc  <  1:562656/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                  AGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGC  >  NC_000913/2353072‑2353649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑