Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,954	C→A	N441K (AAC→AAA)	radD →	putative DNA repair helicase RadD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,954	0	C	A	100.0%	211.6 / NA	57	N441K (AAC→AAA)	radD	putative DNA repair helicase RadD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/31);  total (26/31)

GTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGAT  >  NC_000913/2281659‑2282244                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                   gTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGata                                                                                                                                                                                                                                                                                                >  1:540804/1‑300 (MQ=255)           gggCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGccgccc                                                                                                                                                                                                                                                                                       >  2:619124/1‑299 (MQ=255)           gggCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGccgccc                                                                                                                                                                                                                                                                                       >  1:419844/1‑299 (MQ=255)                aGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCt                                                                                                                                                                                                                                                                                 >  1:461055/1‑300 (MQ=255)                aGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCACTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGACCGCCGCt                                                                                                                                                                                                                                                                                 >  2:325563/1‑300 (MQ=255)                aGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGNTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTTGGGTAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCt                                                                                                                                                                                                                                                                                 <  2:555909/300‑1 (MQ=255)                              aTTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGac                                                                                                                                                                                                                                                                   >  1:495774/1‑300 (MQ=255)                                tCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCAGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGTGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGacac                                                                                                                                                                                                                                                                 <  2:404940/300‑1 (MQ=255)                                     aTTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTGTGGGGGAAAACGACCGCNGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCATGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTAc                                                                                                                                                                                                                                                            <  2:229689/300‑1 (MQ=255)                                          gCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGtt                                                                                                                                                                                                                                                       <  2:38806/300‑1 (MQ=255)                                          gCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGtt                                                                                                                                                                                                                                                       <  1:39112/300‑1 (MQ=255)                                          gCGGGTAATACGCACAATCTCTGCGAGCCGGAAGTTGGTACACCAAAAGGCAAAGGTGACAGCGGTCCGGTACAGGTTTTCTGCCTTGCCTGCGGTGTTGCCAACACCTTTCGTGGGAAAACGACCGCCGACTGGACATTAAGTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCAAACAATGTGACTTCCTGTTCCGTTTTAAAAATTGCCCTCAATGTAACGCGGAAAAAGATATTGCCGCCCGTCGCTGCCGTGAATTTGACACCGTACTGGtt                                                                                                                                                                                                                                                       <  2:16311/300‑1 (MQ=255)                                            gggTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAACTTGCCCGCAATGTAAAGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGa                                                                                                                                                                                                                                                     >  2:157397/1‑300 (MQ=255)                                                           tctcTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGtt                                                                                                                                                                                                                                      >  1:692573/1‑300 (MQ=255)                                                                acgcgccgtaagtctatACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGTTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGACATGTTAAAAg                                                                                                                                                                                                                                 <  2:187628/284‑1 (MQ=255)                                                                                 acacCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGTACGATATGTTAAAAGCGGCGCTACGACTGaaa                                                                                                                                                                                                                <  2:103827/300‑1 (MQ=255)                                                                                              aaaGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTAt                                                                                                                                                                                                   <  1:13123/300‑1 (MQ=255)                                                                                                  tGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACg                                                                                                                                                                                               <  1:703409/300‑1 (MQ=255)                                                                                                   gACAACGTTCCGGTACGGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACTGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGc                                                                                                                                                                                              <  2:361380/300‑1 (MQ=255)                                                                                                   gACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTTAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGc                                                                                                                                                                                              <  1:1094/300‑1 (MQ=255)                                                                                                   gACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGc                                                                                                                                                                                              <  1:23189/300‑1 (MQ=255)                                                                                                   gACAACGTTCCGGTACAAGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTTAAAGACGCGCTGGTATTACGc                                                                                                                                                                                              <  2:302525/300‑1 (MQ=255)                                                                                                        cGTTCCTGTACAGGTTTTCTGCCCTTCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTTGCCGTCGCTGTCAGGGATGGTTGGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGTCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGc                                                                                                                                                                                              <  2:76242/295‑1 (MQ=255)                                                                                                           tCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCTTCGCTGTCATGGATGGTTTGAAGAGGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCgg                                                                                                                                                                                      <  2:403620/300‑1 (MQ=255)                                                                                                               gTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCAt                                                                                                                                                                                   >  1:615613/1‑299 (MQ=255)                                                                                                                    ggTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCttt                                                                                                                                                                             >  2:609080/1‑300 (MQ=255)                                                                                                                    ggTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCttt                                                                                                                                                                             >  1:115444/1‑300 (MQ=255)                                                                                                                     gTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCttt                                                                                                                                                                             >  1:125776/1‑299 (MQ=255)                                                                                                                     gTTTTCTACCCTGCCTGCGGTTTTGCCAACAGCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGGACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTNGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGTGGCATGTCttt                                                                                                                                                                             >  1:519919/1‑299 (MQ=255)                                                                                                                        ttCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCaa                                                                                                                                                                         <  1:93774/300‑1 (MQ=255)                                                                                                                                 gcctgcGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTACGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCaa                                                                                                                                                                         >  2:298740/1‑291 (MQ=255)                                                                                                                                       ggTTTTGCCAACACCTTTTAGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGAAcgacga                                                                                                                                                           >  2:167698/1‑299 (MQ=255)                                                                                                                                         ttttttcAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTTGCCGTCGCTGTCAGGGATGGTTGGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGaa                                                                                                                                                        <  2:383413/294‑1 (MQ=255)                                                                                                                                                                           gACGGGACATTGATTGAACATTTTGGCCGTCGCTGTCAGTGATGGTTTTAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCTGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGTTTGAAAATCACCTATTACGATg                                                                                                                        <  2:190473/298‑1 (MQ=255)                                                                                                                                                                              tgtaCATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGAc                                                                                                                   <  2:640096/297‑1 (MQ=255)                                                                                                                                                                                      gattggacgcttgggccgtcgCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCTGTCTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCTGGACGATATGTTAAAAGTGGCGCTACGACTGAAAGGCGCGCTGGTATTAAGCTGTAGCGGCATGTCTTTGCCACATGGGCac                                                                                                                                                                >  2:373428/17‑247 (MQ=255)                                                                                                                                                                                      gattgaACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCACTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGCAAATCACCTATTACGATGAAGCCGGTGCGGa                                                                                                           >  2:373016/1‑300 (MQ=255)                                                                                                                                                                                        ttgaACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCNTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGAtg                                                                                                         <  1:140898/300‑1 (MQ=255)                                                                                                                                                                                              acTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATgtgagtg                                                                                                   <  1:497531/300‑1 (MQ=255)                                                                                                                                                                                              acTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATgtgagt                                                                                                    <  2:600767/299‑1 (MQ=255)                                                                                                                                                                                                  tggccgtcgCTGTCATGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCg                                                                                               <  1:675108/296‑1 (MQ=255)                                                                                                                                                                                                  tggccgtcgCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCg                                                                                               <  1:674680/296‑1 (MQ=255)                                                                                                                                                                                                          gCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTCACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTCAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGAATGTCTTTGCAAAATGGGCACGACAAGAAAGGCGAATGGTTGAAAATcgggccctt                                                                                                                             >  1:242485/1‑254 (MQ=255)                                                                                                                                                                                                             gTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGc                                                                                    >  1:285022/1‑300 (MQ=255)                                                                                                                                                                                                             gTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCAACTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGc                                                                                    >  1:285151/1‑300 (MQ=255)                                                                                                                                                                                                             gTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCAACTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGc                                                                                    >  2:624660/1‑300 (MQ=255)                                                                                                                                                                                                             gTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATCGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGTGGATGTGAGTGAGCGTTTCCGTCTGc                                                                                    >  1:583520/1‑300 (MQ=255)                                                                                                                                                                                                                      ggTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCAACTCTTACGCTGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCt                                                                            >  1:187297/1‑299 (MQ=255)                                                                                                                                                                                                                      ggTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTACGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTAAAACGAAGACGGTGCGGATGTGAGTGAGCGTTTCCGTCTGCCAACACCt                                                                            >  2:68476/1‑299 (MQ=255)                                                                                                                                                                                                                         ttGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCTTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGccc                                                                        <  2:207519/300‑1 (MQ=255)                                                                                                                                                                                                                         ttGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGccc                                                                        <  1:472864/300‑1 (MQ=255)                                                                                                                                                                                                                                    gacgGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGAGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGc                                                              <  1:609080/299‑1 (MQ=255)                                                                                                                                                                                                                                           aTCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGagc                                                      <  2:461055/300‑1 (MQ=255)                                                                                                                                                                                                                                                     aTGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTAt                                            >  1:695398/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCcgc            <  1:619124/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                         aTGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCcgctgc         >  1:28777/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                   gAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTTGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGAt  <  2:460433/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                   GTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTTTTATCCGCCCGCATACGCGCACACCGGGCATCCCGCTGCGCTGGAT  >  NC_000913/2281659‑2282244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑