Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,855	T→C	G408G (GGT→GGC)	radD →	putative DNA repair helicase RadD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,855	0	T	C	100.0%	219.7 / NA	59	G408G (GGT→GGC)	radD	putative DNA repair helicase RadD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/35);  total (24/35)

GTACTGACCACCGGATTTGACGCCCCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTG  >  NC_000913/2281556‑2282147                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                     gTACTGACCACCGGATTTGACGCCCCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGA‑‑‑‑‑‑‑CTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCt                                                                                                                                                                                                                                                                                               >  2:140898/1‑300 (MQ=255)       accaccGGATTTGACGCCCCGCACTTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGc                                                                                                                                                                                                                                                                                                <  1:611931/300‑1 (MQ=255)       accaccGGATTTGACGCCCCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGc                                                                                                                                                                                                                                                                                                <  1:611925/300‑1 (MQ=255)               aTTTGACGCCCCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGTNCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACTGGACATTGATTGAACACTTTGGCCGTCGCTGTCAggg                                                                                                                                                                                                                                                                                        <  1:147441/300‑1 (MQ=255)               aTTTGACGCCCCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGNCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAggg                                                                                                                                                                                                                                                                                        <  1:147829/300‑1 (MQ=255)                           gCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAg                                                                                                                                                                                                                                                                             <  2:680812/299‑1 (MQ=255)                           gCACGTCAATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGTGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAg                                                                                                                                                                                                                                                                             <  2:73809/299‑1 (MQ=255)                                         tCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATc                                                                                                                                                                                                                                                              >  1:361380/1‑300 (MQ=255)                                              aTTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGaa                                                                                                                                                                                                                                                         >  2:675108/1‑300 (MQ=255)                                              aTTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTAGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGaa                                                                                                                                                                                                                                                         >  2:674680/1‑300 (MQ=255)                                               ttCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAAc                                                                                                                                                                                                                                                        <  1:60586/300‑1 (MQ=255)                                                        cTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTAGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACt                                                                                                                                                                                                                                               >  1:190473/1‑300 (MQ=255)                                                         tACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCTTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGAc                                                                                                                                                                                                                                                <  1:694513/298‑1 (MQ=255)                                                                 cAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACttccgtttcc                                                                                                                                                                                                                                      >  1:375764/1‑300 (MQ=255)                                                                                           gtgcgcGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTaa                                                                                                                                                                                                             <  1:106072/297‑1 (MQ=255)                                                                                           gggCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTaa                                                                                                                                                                                                             <  1:105223/299‑1 (MQ=255)                                                                                             gcgcGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGNACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAAcgc                                                                                                                                                                                                          <  2:561190/300‑1 (MQ=255)                                                                                                  gTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAAcgc                                                                                                                                                                                                          >  1:478739/1‑295 (MQ=255)                                                                                                  gTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAAcgc                                                                                                                                                                                                          <  2:478739/295‑1 (MQ=255)                                                                                                  gTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGNTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGaaa                                                                                                                                                                                                     >  1:513041/1‑300 (MQ=255)                                                                                                        gTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGata                                                                                                                                                                                               >  1:540804/1‑300 (MQ=255)                                                                                                                  gggCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGccgccc                                                                                                                                                                                      >  1:419844/1‑299 (MQ=255)                                                                                                                  gggCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGccgccc                                                                                                                                                                                      >  2:619124/1‑299 (MQ=255)                                                                                                                       aGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCt                                                                                                                                                                                >  1:461055/1‑300 (MQ=255)                                                                                                                       aGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCACTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGACCGCCGCt                                                                                                                                                                                >  2:325563/1‑300 (MQ=255)                                                                                                                       aGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGNTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTTGGGTAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCt                                                                                                                                                                                <  2:555909/300‑1 (MQ=255)                                                                                                                                     aTTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGac                                                                                                                                                                  >  1:495774/1‑300 (MQ=255)                                                                                                                                       tCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCAGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGTGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGacac                                                                                                                                                                <  2:404940/300‑1 (MQ=255)                                                                                                                                            aTTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTGTGGGGGAAAACGACCGCNGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCATGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTAc                                                                                                                                                           <  2:229689/300‑1 (MQ=255)                                                                                                                                                 gCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGtt                                                                                                                                                      <  2:38806/300‑1 (MQ=255)                                                                                                                                                 gCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGtt                                                                                                                                                      <  1:39112/300‑1 (MQ=255)                                                                                                                                                 gCGGGTAATACGCACAATCTCTGCGAGCCGGAAGTTGGTACACCAAAAGGCAAAGGTGACAGCGGTCCGGTACAGGTTTTCTGCCTTGCCTGCGGTGTTGCCAACACCTTTCGTGGGAAAACGACCGCCGACTGGACATTAAGTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCAAACAATGTGACTTCCTGTTCCGTTTTAAAAATTGCCCTCAATGTAACGCGGAAAAAGATATTGCCGCCCGTCGCTGCCGTGAATTTGACACCGTACTGGtt                                                                                                                                                      <  2:16311/300‑1 (MQ=255)                                                                                                                                                   gggTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAACTTGCCCGCAATGTAAAGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGa                                                                                                                                                    >  2:157397/1‑300 (MQ=255)                                                                                                                                                                  tctcTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGtt                                                                                                                                     >  1:692573/1‑300 (MQ=255)                                                                                                                                                                       acgcgccgtaagtctatACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGTTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGACATGTTAAAAg                                                                                                                                <  2:187628/284‑1 (MQ=255)                                                                                                                                                                                        acacCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGTACGATATGTTAAAAGCGGCGCTACGACTGaaa                                                                                                               <  2:103827/300‑1 (MQ=255)                                                                                                                                                                                                     aaaGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTAt                                                                                                  <  1:13123/300‑1 (MQ=255)                                                                                                                                                                                                         tGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACg                                                                                              <  1:703409/300‑1 (MQ=255)                                                                                                                                                                                                          gACAACGTTCCGGTACGGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACTGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGc                                                                                             <  2:361380/300‑1 (MQ=255)                                                                                                                                                                                                          gACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTTAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGc                                                                                             <  1:1094/300‑1 (MQ=255)                                                                                                                                                                                                          gACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGc                                                                                             <  1:23189/300‑1 (MQ=255)                                                                                                                                                                                                          gACAACGTTCCGGTACAAGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTTAAAGACGCGCTGGTATTACGc                                                                                             <  2:302525/300‑1 (MQ=255)                                                                                                                                                                                                               cGTTCCTGTACAGGTTTTCTGCCCTTCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTTGCCGTCGCTGTCAGGGATGGTTGGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGTCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGc                                                                                             <  2:76242/295‑1 (MQ=255)                                                                                                                                                                                                                  tCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCTTCGCTGTCATGGATGGTTTGAAGAGGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCgg                                                                                     <  2:403620/300‑1 (MQ=255)                                                                                                                                                                                                                      gTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCAt                                                                                  >  1:615613/1‑299 (MQ=255)                                                                                                                                                                                                                           ggTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCttt                                                                            >  2:609080/1‑300 (MQ=255)                                                                                                                                                                                                                           ggTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCttt                                                                            >  1:115444/1‑300 (MQ=255)                                                                                                                                                                                                                            gTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCttt                                                                            >  1:125776/1‑299 (MQ=255)                                                                                                                                                                                                                            gTTTTCTACCCTGCCTGCGGTTTTGCCAACAGCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGGACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTNGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGTGGCATGTCttt                                                                            >  1:519919/1‑299 (MQ=255)                                                                                                                                                                                                                               ttCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCaa                                                                        <  1:93774/300‑1 (MQ=255)                                                                                                                                                                                                                                        gcctgcGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTACGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCaa                                                                        >  2:298740/1‑291 (MQ=255)                                                                                                                                                                                                                                              ggTTTTGCCAACACCTTTTAGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGAAcgacga                                                          >  2:167698/1‑299 (MQ=255)                                                                                                                                                                                                                                                ttttttcAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTTGCCGTCGCTGTCAGGGATGGTTGGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGaa                                                       <  2:383413/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gACGGGACATTGATTGAACATTTTGGCCGTCGCTGTCAGTGATGGTTTTAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCTGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGTTTGAAAATCACCTATTACGATg                       <  2:190473/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tgtaCATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGAc                  <  2:640096/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gattgaACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCACTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGCAAATCACCTATTACGATGAAGCCGGTGCGGa          >  2:373016/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               ttgaACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCNTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGAtg        <  1:140898/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     acTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATgtgagtg  <  1:497531/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     acTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATgtgagt   <  2:600767/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                     GTACTGACCACCGGATTTGACGCCCCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTG  >  NC_000913/2281556‑2282147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑