Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,932,745	C→T	W4* (TGG→TGA)	eda ←	KHG/KDPG aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,932,745	0	C	T	100.0%	191.2 / NA	53	W4* (TGG→TGA)	eda	KHG/KDPG aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/25);  total (28/25)

GTCAGACCCGGGCTAATTGCGAACTGTGCACCCGCTTCAGTGACTTCTGCCAGCTGCTGTGGATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTCCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTG  >  NC_000913/1932467‑1933028                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                            gTCAGACCCGGGCTAATTGCGAACTGTGCACCCGCTTCAGTGACTTCTGCCAGCTGCTGTGGATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTtc                                                                                                                                                                                                                                                                        >  1:430763/1‑300 (MQ=255)         caggccaatcgCGAACTGTGCACCCGCTTCAGTGACTTCTGCCAGCTGCTGTGGATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAAAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCt                                                                                                                                                                                                                                                                >  2:654361/11‑300 (MQ=255)                                            cTTCTGCCCGCTGCTGTGGATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATCACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGCTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACa                                                                                                                                                                                                                               >  1:408691/1‑298 (MQ=255)                                            cTTCTGCCAGCTGCTGTGGATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACa                                                                                                                                                                                                                               >  1:408743/1‑298 (MQ=255)                                             ttCTGCCAGCTGCTGTGGATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGt                                                                                                                                                                                                                            <  1:274649/300‑1 (MQ=255)                                             ttCTGCCAGCTGCTGTGGATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGAGAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGt                                                                                                                                                                                                                            <  1:274650/300‑1 (MQ=255)                                                          tgtgGATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTc                                                                                                                                                                                                                <  1:237685/299‑1 (MQ=255)                                                              gATTCAGCACCGTACCGGCACCCACAATCGCTTCATGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCAc                                                                                                                                                                                                           <  2:567716/300‑1 (MQ=255)                                                              gATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCAc                                                                                                                                                                                                           <  2:567664/300‑1 (MQ=255)                                                              gATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCAc                                                                                                                                                                                                           <  1:702812/300‑1 (MQ=255)                                                               aTTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCAcc                                                                                                                                                                                                          >  1:178390/1‑300 (MQ=255)                                                               aTTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCAcc                                                                                                                                                                                                          >  2:487768/1‑300 (MQ=255)                                                               aTTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCAACGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAAACGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTc                                                                                                                                                                                                                >  2:62874/1‑294 (MQ=255)                                                                       ccGTACCGGCACCCACAATCGCTTAAGGCACTTCTTTGGCGATAGCACGGATAGTGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAAAGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCAAGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTATCGAATGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTACGCCCTGTTCGGCACCGGACAGt                                                                                                                                                                                                   >  2:151869/1‑299 (MQ=255)                                                                              ggCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCAc                                                                                                                                                                                            >  1:337784/1‑299 (MQ=255)                                                                              ggCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTACAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCa                                                                                                                                                                                             >  1:337520/1‑298 (MQ=255)                                                                               gCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAATTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAACAACCAACACTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTCTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACTGGACAGTTTTTCACGc                                                                                                                                                                                          <  1:498941/300‑1 (MQ=255)                                                                               gCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGc                                                                                                                                                                                          <  1:498579/300‑1 (MQ=255)                                                                                                aGGCACTTCTTTGGCGACAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTc                                                                                                                                                                         <  1:240958/300‑1 (MQ=255)                                                                                                    aCTTCTTTGGCAATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCGGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCAc                                                                                                                                                                       <  2:39829/298‑1 (MQ=255)                                                                                                          ttGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCAACGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGtt                                                                                                                                                               <  1:62874/300‑1 (MQ=255)                                                                                                                aTAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCAcg                                                                                                                                                         <  1:165989/300‑1 (MQ=255)                                                                                                                aTAGCACAGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCTGTACAACCGGGCCGGTGGTCAGGATTGATTCGGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCAcg                                                                                                                                                         <  2:338027/300‑1 (MQ=255)                                                                                                                            gCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCtc                                                                                                                                             >  1:653132/1‑300 (MQ=255)                                                                                                                                       acacTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCTGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGaa                                                                                                                                   <  1:97177/299‑1 (MQ=255)                                                                                                                                       acacTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAACAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGaa                                                                                                                                   <  2:619702/299‑1 (MQ=255)                                                                                                                                       acacTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGaa                                                                                                                                   <  1:272419/299‑1 (MQ=255)                                                                                                                                         acTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCAGCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTAGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCGGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGaa                                                                                                                                   <  2:113720/297‑1 (MQ=255)                                                                                                                                          cTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATCACCGGTACAACCGGGCAGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAATGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAAtgt                                                                                                                                >  2:253064/1‑299 (MQ=255)                                                                                                                                           tCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGc                                                                                                                              <  1:579404/300‑1 (MQ=255)                                                                                                                                            cGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGATTTTGCAATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAAACGGGCAGGTGGTCAGGATTGATTCTGCATTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTGGNCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAAGTCAGGAACGTGc                                                                                                                              >  2:228202/1‑299 (MQ=255)                                                                                                                                                     gagTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCAGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAACTTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGTGGTTCGCGa                                                                                                                     >  2:126596/1‑299 (MQ=255)                                                                                                                                                       gTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGagca                                                                                                                  >  2:34192/1‑300 (MQ=255)                                                                                                                                                           cTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCa                                                                                                              >  1:111196/1‑300 (MQ=255)                                                                                                                                                                    cgcgCACCCCACCAGCAACCAACGCTTTTGCCCTCGGCACCGCGTGTTCCAGTTTTTTTCCCACGATAACCGGTACAAACGGGCCGGTGGTCAGGATTGATTCTGCCCTTGTTTTTCAGTTTTTCATCAGAGTTTTCTATAGACTGATTACAAATTTGTCGTCTTCAAAAGTGATACAGGTTGCGCACTGTTCGACACCGGACAGTTTTTCAAGCAAGGCGCCGAATAATTCACGTCATGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGTAGCCAGTTCCGCtt                                                                                                     >  2:111147/1‑300 (MQ=255)                                                                                                                                                                        cACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCGCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTc                                                                                                 >  2:485945/1‑300 (MQ=255)                                                                                                                                                                          ccccACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTa                                                                                               >  1:38027/1‑300 (MQ=255)                                                                                                                                                                                 aGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACcagc                                                                                          <  1:164340/298‑1 (MQ=255)                                                                                                                                                                                                 ggaatgggCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTAGCCTGATTACACATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAATTTTTCACGCAAGGCGCTGAATACTTCACGTCCTGTTCCCCCGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGCTCGc                                                                             >  2:630805/7‑295 (MQ=255)                                                                                                                                                                                                   cATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGc                                                                             >  1:21713/1‑293 (MQ=255)                                                                                                                                                                                                       ggCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGTTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTcc                                                                   <  2:111196/299‑1 (MQ=255)                                                                                                                                                                                                                  ttCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAat                                                       >  1:372886/1‑300 (MQ=255)                                                                                                                                                                                                                             ttACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGt                                            <  1:34192/300‑1 (MQ=255)                                                                                                                                                                                                                                               cAACCGGGCAGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCATGTCTGTCCATTCACACGAATGATGTCCACGTCGCGCACTTTTGCcagca                          >  2:1939/1‑300 (MQ=255)                                                                                                                                                                                                                                                acccGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCACTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCcagca                          >  2:321045/3‑299 (MQ=255)                                                                                                                                                                                                                                                aaCCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTAAGTTCGCCTGTCTGTCCATTCACACGAATGATGTACACGTCGCGCACTTTTGCcagca                          >  2:321377/1‑299 (MQ=255)                                                                                                                                                                                                                                                aaCCGGACCGGTGGTCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTAACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCATGTCTGTCCactc                                                               >  2:403787/1‑259 (MQ=255)                                                                                                                                                                                                                                                         ggtggtCAGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACAAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCAt                 <  1:581019/299‑1 (MQ=255)                                                                                                                                                                                                                                                                aGGATTGATTCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGc          <  1:655858/299‑1 (MQ=255)                                                                                                                                                                                                                                                                    tcgattCTGCACTTGTTTTTCAGTTTTTCATCAGGGTTTTCCCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCTGCACCGGACAGTTTTTCACGCAATGCGCTGAATAATTCATGTCCTGTTCCCACGCGTGACGCGCTCAGGTCATGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGCCGCGCACTTTTGCCAGCAGCCCGCCATCggag            <  2:690912/291‑4 (MQ=255)                                                                                                                                                                                                                                                                       attCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGgtg  <  1:619961/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       attCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCAAGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGgt   >  2:453026/1‑299 (MQ=255)                                                                                                                                                                                                                                                                       attCTGCACTTGTTTTTCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCATGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCAATCGCAGGCTTCTGgt   >  2:97072/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                            GTCAGACCCGGGCTAATTGCGAACTGTGCACCCGCTTCAGTGACTTCTGCCAGCTGCTGTGGATTCAGCACCGTACCGGCACCCACAATCGCTTCAGGCACTTCTTTGGCGATAGCACGGATAGCGTCAACTGCACACTCGGTACGCAGAGTCACTTCCAGAACGCGCACCCCACCAGCAACCAACGCTTTTGCCATCGGCACCGCGTGTTCCAGTTTTTTTACCACGATAACCGGTACAACCGGGCCGGTGGTCAGGATTGATTCTGCACTTGTTTTCCAGTTTTTCATCAGAGTTTTCTCTCGCCTGATTACAAATTTGTCGTCTTAAAAAGTGATACAGGTTGCGCCCTGTTCGGCACCGGACAGTTTTTCACGCAAGGCGCTGAATAATTCACGTCCTGTTCCCACGCGTGACGCGCTCAGGTCAGGAATGTGCGGTTCGCGAGCAGCCAGTTCCGCTTCGTCTACCAGCAGCGTCAGTTCGCCTGTCTGTCCATTCACACGAATGATGTCCCCGTCGCGCACTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTG  >  NC_000913/1932467‑1933028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑