Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,797,083	C→T	V288M (GTG→ATG)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,797,083	0	C	T	100.0%	130.6 / NA	41	V288M (GTG→ATG)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/14);  total (27/14)

GGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGAT  >  NC_000913/1796813‑1797371                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                 gggTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGc                                                                                                                                                                                                                                                                      >  1:368578/1‑299 (MQ=255)                       gtgCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAAt                                                                                                                                                                                                                                                  >  1:192932/1‑297 (MQ=255)                               gTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTAt                                                                                                                                                                                                                                       >  1:272125/1‑300 (MQ=255)                                     gATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGaaa                                                                                                                                                                                                                                  >  1:558763/1‑299 (MQ=255)                                                 gCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGTACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCAAGTGCCTTGTTGTGGTCGGCGATGAACAGAGTGTCAGCATTCAGCTTAGCTTCAGTACCGTCGAGCAGCATCCGCGTTTCGACCTATTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTCTCGAGAGCGTGCATAGGCt                                                                                                                                                                                                                     >  2:702328/1‑300 (MQ=255)                                                    cATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGc                                                                                                                                                                                                                  >  1:210744/1‑300 (MQ=255)                                                      tAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGccc                                                                                                                                                                                                                >  1:97786/1‑300 (MQ=255)                                                                        gTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTCCCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGAACCACAATGCCGACTTCAATGCGATCTTTATTGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATa                                                                                                                                                                                               >  2:16251/1‑299 (MQ=255)                                                                        gTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTNCGCCACCGAAGATGCCGCCCATCGCCAGCGCCNTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTAAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGACTTCAATGTGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATa                                                                                                                                                                                               >  2:544840/1‑299 (MQ=255)                                                                         tatgcatttcATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGATCTCGCATTCCGGCAGCACGTTTTGTGTTTCGTCAGTCACGCCAGAGTGTTTGCCACCGGAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCCTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGt                                                                                                                                                                                             <  2:600218/291‑1 (MQ=255)                                                                         tATGCAGGCCATGACGACGAGCACGACCGTTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCANGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGt                                                                                                                                                                                             <  2:559465/300‑1 (MQ=255)                                                                         tATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGt                                                                                                                                                                                             <  1:77499/300‑1 (MQ=255)                                                                         tATGCAGGCCATGACGACAAGCACGACCTGTGATAGACAGCGGGCTAAAGAACACGCATTCCAGCAGCACGTTTTGTGCTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGt                                                                                                                                                                                             <  1:9303/300‑1 (MQ=255)                                                                                     gacgacGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCTCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTcaa                                                                                                                                                                                 >  1:220610/1‑300 (MQ=255)                                                                                              cACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCATATAGTTGGTGACGCCAACAACTGCAt                                                                                                                                                                        >  2:249174/1‑300 (MQ=255)                                                                                                 gACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGa                                                                                                                                                                     >  1:538534/1‑300 (MQ=255)                                                                                                  aCCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGAt                                                                                                                                                                    >  1:287800/1‑300 (MQ=255)                                                                                                      gTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGaa                                                                                                                                                                >  1:274021/1‑300 (MQ=255)                                                                                                                  gggCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCaa                                                                                                                                                    >  1:557583/1‑300 (MQ=255)                                                                                                                      tAAAGAACGCGCGTTCCAGCAGCGCTTTTTGTGTTTCTTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCAGCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGTTGACGTCAACAACTGCATCGATAGAACGGACCCCGCAacga                                                                                                                                                 <  2:404566/299‑1 (MQ=255)                                                                                                                                 cATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTtttct                                                                                                                                     >  1:129333/1‑300 (MQ=255)                                                                                                                                  aTTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTtttctt                                                                                                                                    <  2:663368/300‑1 (MQ=255)                                                                                                                                                         tCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCt                                                                                                             <  1:642912/300‑1 (MQ=255)                                                                                                                                                               ttCACGCCAGATTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACg                                                                                                       <  2:249538/300‑1 (MQ=255)                                                                                                                                                               ttCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACg                                                                                                       <  2:249675/300‑1 (MQ=255)                                                                                                                                                                  aCGCCAGAGTGTTCGCNACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTNTCTTTCATCCACAGCGGANTTGGCGCTTTAACGTTa                                                                                                    >  1:589692/1‑300 (MQ=255)                                                                                                                                                                     ccAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCCGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAAt                                                                                                  >  2:284310/1‑299 (MQ=255)                                                                                                                                                                          gtgtTCGCCACCGAAGGTGCCGCCCATCGCCAGTGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCGTCGCTTCCGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGACATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAATTCGAGCAGCGCATAGTTGGTGAAGTCAACAACTGCATCGATAGAACGGATCCCGCACCGACGCAGTTTTTCTTTACTCCCCCACGGAGTTGGCGCTTTGACGttgcttc                                                                                                >  2:206814/1‑291 (MQ=255)                                                                                                                                                                                gCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACcac                                                                                      <  2:499522/300‑1 (MQ=255)                                                                                                                                                                                   aCCGAAGATGCCGCCCACCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCacac                                                                                    <  1:60741/299‑1 (MQ=255)                                                                                                                                                                                                            gcCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTCACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTcc                                                          >  1:545838/1‑300 (MQ=255)                                                                                                                                                                                                            gcCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTcc                                                          >  1:545759/1‑300 (MQ=255)                                                                                                                                                                                                               ttgttgTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCgac                                                       >  2:76268/1‑298 (MQ=255)                                                                                                                                                                                                               ttgttgTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTCCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTCGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGgc                                                       <  2:404715/300‑1 (MQ=255)                                                                                                                                                                                                                              aTGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAAAGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATc                                        <  1:249102/300‑1 (MQ=255)                                                                                                                                                                                                                                 aCCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGc                                     <  1:16251/300‑1 (MQ=255)                                                                                                                                                                                                                                                          cttcAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGNCGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTCTTACCACACGGAAAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCa             >  2:235050/1‑299 (MQ=255)                                                                                                                                                                                                                                                          cttcAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCa             >  2:348776/1‑299 (MQ=255)                                                                                                                                                                                                                                                               gTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGa       >  2:414445/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gTACCATCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCCTCCACAGCGGAGTTGGCGCTTTAACGTTACTGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTCATCGGCAGCGTGTCGTCGATGATCGCACCAACCGGa       >  2:414437/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGAt  >  1:425840/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                 GGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGAT  >  NC_000913/1796813‑1797371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑