Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,751,024	C→A	intergenic (‑11/+53)	ydhX ← / ← ydhW	putative 4Fe‑4S ferredoxin‑like protein YdhX/uncharacterized protein YdhW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,751,024	0	C	A	100.0%	181.9 / NA	51	intergenic (‑11/+53)	ydhX/ydhW	putative 4Fe‑4S ferredoxin‑like protein YdhX/uncharacterized protein YdhW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/22);  total (29/22)

GTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCAC  >  NC_000913/1750732‑1751317                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      gTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTNCAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGc                                                                                                                                                                                                                                                                                                >  2:525405/1‑300 (MQ=255) gTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGc                                                                                                                                                                                                                                                                                                >  1:630355/1‑300 (MQ=255)      gACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGt                                                                                                                                                                                                                                                                                             >  1:669841/1‑298 (MQ=255)      gACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTAAAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGCACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGccgt                                                                                                                                                                                                                                                                                             >  1:671055/1‑295 (MQ=255)      gACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCATGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGt                                                                                                                                                                                                                                                                                             >  1:671835/1‑298 (MQ=255)               ggCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGACTGCAAc                                                                                                                                                                                                                                                                                    <  1:294956/298‑1 (MQ=255)                                tGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGAc                                                                                                                                                                                                                                                                 <  1:61816/300‑1 (MQ=255)                                tGCGTTTCGTTGTCGGTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACCGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTCTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGAc                                                                                                                                                                                                                                                                 <  2:18422/300‑1 (MQ=255)                                 gCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACa                                                                                                                                                                                                                                                                >  1:60930/1‑300 (MQ=255)                                           gTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGaa                                                                                                                                                                                                                                                      >  1:424569/1‑300 (MQ=255)                                                         cTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCt                                                                                                                                                                                                                                        >  2:649421/1‑300 (MQ=255)                                                                 tgtgCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACCCCTGATTATCCACCAATGCAGTGGCTGCAACTGTCCACCATATCCATGACAGCTCAATGGAATTTCAGGGGTTCTGAAACTGc                                                                                                                                                                                                                                >  2:66182/1‑300 (MQ=255)                                                                       aTCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATACATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTAcc                                                                                                                                                                                                                          >  1:452683/1‑300 (MQ=255)                                                                        tCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACATAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCt                                                                                                                                                                                                                         <  2:452683/300‑1 (MQ=255)                                                                                     tccatGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTACTGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTACATGAAATTTAAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGc                                                                                                                                                                                                             >  2:201455/5‑299 (MQ=255)                                                                                       ccTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGcata                                                                                                                                                                                                          >  1:204982/1‑300 (MQ=255)                                                                                       ccTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGAAATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACAATTCGcata                                                                                                                                                                                                          >  1:562179/1‑300 (MQ=255)                                                                                          gAGCGGGGACGTGGTTNGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGNTCTGAAACTGCTCTACCTNGAACCATTCGcataagc                                                                                                                                                                                                       >  1:586018/1‑300 (MQ=255)                                                                                          gAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGcataagc                                                                                                                                                                                                       >  1:344466/1‑300 (MQ=255)                                                                                                           gTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATa                                                                                                                                                                                       >  1:669540/1‑299 (MQ=255)                                                                                                           gTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCCCCCTGAAATTTCAGGGGTTCTGAAATTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATa                                                                                                                                                                                       >  1:669583/1‑299 (MQ=255)                                                                                                           gTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCAACCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCAt                                                                                                                                                                                        >  2:694217/1‑298 (MQ=255)                                                                                                                     aTGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAt                                                                                                                                                                             <  1:433934/299‑1 (MQ=255)                                                                                                                                   atatTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACaa                                                                                                                                                               >  1:496609/1‑299 (MQ=255)                                                                                                                                   atatTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTACCTGAAATTTCAGGGGTTCTGAAACTGCTCTAACTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACaa                                                                                                                                                               >  2:371433/1‑299 (MQ=255)                                                                                                                                   atatTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑G‑CGCTAACAGAGAAGACGT‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACAGCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAg                                                                                                                                                              <  1:98545/300‑1 (MQ=255)                                                                                                                                    tatTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGt                                                                                                                                                             >  1:634104/1‑300 (MQ=255)                                                                                                                                     atTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTa                                                                                                                                                            >  1:371158/1‑300 (MQ=255)                                                                                                                                           gCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAacac                                                                                                                                                      <  1:571403/300‑1 (MQ=255)                                                                                                                                           gCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGNGGCTGCAACTGTCCACCCTATCCATGACATCTNCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTTCCATAGGTCATAAATCGCTCGCTAAACAAGTACAacac                                                                                                                                                      <  2:527888/300‑1 (MQ=255)                                                                                                                                                      gTGACTCGTCATGAATNATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATANATCGCTCGCTAAACAAGTNCAACACCGCCGTCGAt                                                                                                                                            >  1:600202/1‑299 (MQ=255)                                                                                                                                                      gTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGAt                                                                                                                                            >  1:257581/1‑299 (MQ=255)                                                                                                                                                              tCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCAcc                                                                                                                                   <  2:685917/300‑1 (MQ=255)                                                                                                                                                                   aaTCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACNGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCg                                                                                                                              <  1:525405/300‑1 (MQ=255)                                                                                                                                                                        tGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTatat                                                                                                                         <  1:627070/300‑1 (MQ=255)                                                                                                                                                                                        tttctCTTGCCTCGTGTNT‑CGCTAACAGAGAAGACGN‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATNTCGGCATATTCAGGTg                                                                                                          <  1:588242/296‑1 (MQ=255)                                                                                                                                                                                         tttcgttGCCTCGTGT‑TNCGCTAACAGAGAAGACGCNAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATNTCGGCATATTCAGGTg                                                                                                          <  1:588386/295‑1 (MQ=255)                                                                                                                                                                                                   tCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAcatccat                                                                                                <  2:444709/298‑1 (MQ=255)                                                                                                                                                                                                            cGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTNCCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCTATCGCGGCGAt                                                                                       >  2:226698/1‑300 (MQ=255)                                                                                                                                                                                                            cGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGAt                                                                                       >  1:357490/1‑300 (MQ=255)                                                                                                                                                                                                                        agaCGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAATTAATGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCTACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGTTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCAGATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCaa                                                                            <  2:290400/299‑1 (MQ=255)                                                                                                                                                                                                                                                ttACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAatat                                                     >  2:314816/1‑299 (MQ=255)                                                                                                                                                                                                                                                 tACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATgg                                                   <  1:454426/300‑1 (MQ=255)                                                                                                                                                                                                                                                 tACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATgg                                                   <  2:60930/300‑1 (MQ=255)                                                                                                                                                                                                                                                       tCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACAACGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGATTCAATCTGCGCTTCCTGGAAGTAATATGGCGCtt                                              >  2:673018/1‑299 (MQ=255)                                                                                                                                                                                                                                                           acccccAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATc                                         <  2:669841/298‑1 (MQ=255)                                                                                                                                                                                                                                                           acccccAGAACAAATTTGCGTCGAGTGAAGGACATCTGATGATCCACGAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAGGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAATTAATATGGCGCTTGCATc                                         <  2:671055/298‑1 (MQ=255)                                                                                                                                                                                                                                                                              gTCGAGTGAAGGACATCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCATGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAg                      <  2:212364/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aaGGACAGCTGATTATCCACTAATGCAGTGGCTGCAAAGGGCCACCCTATACATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCACTCGCTAAACAAATACAATACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCaa                                                                            <  2:291232/238‑1 (MQ=255)                                                                                                                                                                                                                                                                                            aTCTGATTATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTc        <  2:630355/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  ttATCCACTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCACTCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCACGGCGACTTTGTAAGGACGGGGATACGTCTGGCAc  >  2:473558/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      GTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAAATATTACAGCCATTGCACCGTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGT‑T‑CGCTAACAGAGAAGACGC‑AGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCAC  >  NC_000913/1750732‑1751317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑