Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,247	C→T	I11I (ATC→ATT)	ydfZ →	putative selenoprotein YdfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,247	0	C	T	100.0%	221.7 / NA	61	I11I (ATC→ATT)	ydfZ	putative selenoprotein YdfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/27);  total (34/27)

ATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTT  >  NC_000913/1628968‑1629528                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                          aTTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGACAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTAATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCACAAAA‑GATGTGAGCTTGATCAAAAACAAAACATATTTCACTCGAAAGGAGTATTTAGATTGCGACCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAAAACCTATGACGACCTACGATCGTAACCGTAACACAATTACCAATGTCAGCCGTGTTAt                                                                                                                                                                                                                                                                       >  2:297661/1‑300 (MQ=255)  tttGCAGGAAAT‑AGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATgg                                                                                                                                                                                                                                                                     <  1:23705/300‑1 (MQ=255)   ttGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATgg                                                                                                                                                                                                                                                                     <  1:204616/300‑1 (MQ=255)     gCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGtt                                                                                                                                                                                                                                                                   <  1:285350/300‑1 (MQ=255)          aaaTCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCgg                                                                                                                                                                                                                                                              <  1:464335/300‑1 (MQ=255)              cAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCAACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAAAAAACACCTATGACGACCTACGATCGTAACCGTCACGCAATTCCCACTGGCAGTCGTGTTATGGTTAGCGGCAcc                                                                                                                                                                                                                                                          >  2:389488/1‑300 (MQ=255)                           cgcCAGATCCGCCAGGNAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGAAAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCNGCCGTGTTATGGTTAGCGNCACCGGTCACACTGGCa                                                                                                                                                                                                                                             >  1:592453/1‑300 (MQ=255)                           cgcCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCTTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCCATGGCAGCCGTCTTCTGGTTAGCGGCACCGGTCACACTGGCa                                                                                                                                                                                                                                             >  2:602749/1‑300 (MQ=255)                                gATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATc                                                                                                                                                                                                                                        <  1:238468/300‑1 (MQ=255)                                                        gTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATNAAAAACAAAAAATATTTCACTCGACAGGAGTANTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCt                                                                                                                                                                                                                 >  2:530340/1‑299 (MQ=255)                                                          ttAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATTGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTANTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAATGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGTTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGAc                                                                                                                                                                                                              <  2:234682/300‑1 (MQ=255)                                                             gCGAAGAGTAATTCATTTACTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCANAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGc                                                                                                                                                                                                           <  1:505397/300‑1 (MQ=255)                                                                  gagTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGc                                                                                                                                                                                                      >  2:486872/1‑300 (MQ=255)                                                                        ttCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATc                                                                                                                                                                                                                                        <  2:433986/260‑1 (MQ=255)                                                                        ttCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATc                                                                                                                                                                                                                                        >  1:433986/1‑260 (MQ=255)                                                                        ttCATTTCCTCTCATCCAATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGAAAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACAACTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCAGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATc                                                                                                                                                                                                                                        >  1:433982/1‑261 (MQ=255)                                                                          cATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATccgc                                                                                                                                                                                              >  2:600867/1‑300 (MQ=255)                                                                           aTTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGTTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATccgc                                                                                                                                                                                              <  1:460604/299‑1 (MQ=255)                                                                           aTTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATccgc                                                                                                                                                                                              <  1:460467/299‑1 (MQ=255)                                                                                    catcccatccGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATAATGGAGGTCTGACCGACGAGCAAATCAGCCGCGGaagaa                                                                                                                                                                                    >  2:114643/1‑297 (MQ=255)                                                                                    catcccatccGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGAAAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGaaaaa                                                                                                                                                                                    >  1:55461/1‑300 (MQ=255)                                                                                         catccGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGAACGCTGAGCAAATCCGCAGCGGAAAAACCGTa                                                                                                                                                                               >  2:95861/1‑300 (MQ=255)                                                                                                     gagTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGt                                                                                                                                                                   <  1:532647/300‑1 (MQ=255)                                                                                                         cTTTTCCCCCGCCTTATGGCTCATGTATGCATCAAAAAG‑GATGTGCGCTTGATCAAAAACAAAAAATATTTCACTCGACAGAAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTCACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCCCCGGTCACACTGGCAAGATCATGTAGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGAGGAAAAACCGTAGTTGTTGAAGGTtgt                                                                                                                                                                >  2:654683/1‑299 (MQ=255)                                                                                                         cTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTtgt                                                                                                                                                                >  2:161489/1‑299 (MQ=255)                                                                                                                    ccTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGNAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATACGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGGAACt                                                                                                                                                     >  2:554970/1‑299 (MQ=255)                                                                                                                    ccTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACt                                                                                                                                                     >  1:628067/1‑299 (MQ=255)                                                                                                                    ccTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACt                                                                                                                                                     >  2:444429/1‑299 (MQ=255)                                                                                                                    ccTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGAAAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGNAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACt                                                                                                                                                     >  2:554964/1‑299 (MQ=255)                                                                                                                    ccTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCAATCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACt                                                                                                                                                     >  2:326786/1‑299 (MQ=255)                                                                                                                           gCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATNCTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGcaccac                                                                                                                                             >  1:155905/1‑300 (MQ=255)                                                                                                                           gCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGCAGTTGTTGAAGGTTGTGCAGAGAAACTggtgccac                                                                                                                                             >  1:250119/1‑294 (MQ=255)                                                                                                                           gCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCAGCGGAAAAACCGTAGTTGTTGAAGGTTGTGCAGAGAAACTGGcaccac                                                                                                                                             >  1:249647/1‑300 (MQ=255)                                                                                                                            cTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACGAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCATGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGCCCGCTGAGCCAATCCGCCGCGGAAAAACCGTAGTTGTTGAAAGTTGTGAAGAGCAACTGGCACCACt                                                                                                                                            >  2:304304/1‑300 (MQ=255)                                                                                                                            cTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACt                                                                                                                                            >  1:360978/1‑300 (MQ=255)                                                                                                                                 gcatgcatCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTGGGTGAGGGTTGTGAGGAGAAACTGGcaggggggggtc                                                                                                                                       <  1:648453/300‑11 (MQ=255)                                                                                                                                 gcatgcatCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATCTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGAcc                                                                                                                                       <  2:657498/300‑1 (MQ=255)                                                                                                                                 gcatgcatCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATACTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACGGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGAcc                                                                                                                                       <  2:571739/300‑1 (MQ=255)                                                                                                                                       atCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGAtt                                                                                                                                  <  2:651675/299‑1 (MQ=255)                                                                                                                                                            gATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGTAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCg                                                                                                             <  1:109734/300‑1 (MQ=255)                                                                                                                                                            gATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCg                                                                                                             <  1:669411/300‑1 (MQ=255)                                                                                                                                                                                             gTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGATTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCAGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGANAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAATTAAGCGTGTGAATGCCGACGATGGCGGAATTGCtttttt                                                                            >  1:143177/1‑300 (MQ=255)                                                                                                                                                                                               aTTTATATTACGCCCGTTACGTGAGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCCAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCAGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTAGGCATGAACTAAGCGTGTGAATGCAGCAGATGGTGGCACTGCTTTTTTAc                                                                          >  2:254991/1‑300 (MQ=255)                                                                                                                                                                                                tttATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGAATTGCTTTTTTACt                                                                         >  2:388135/1‑300 (MQ=255)                                                                                                                                                                                                              gTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTAGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATAtttt                                                           >  2:570320/1‑300 (MQ=255)                                                                                                                                                                                                                            gACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAANACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTNGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCtt                                              <  2:530200/299‑1 (MQ=255)                                                                                                                                                                                                                             aCTGTAAATCAGAAAGTAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTATCGGCACCGTTCACACTTTCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAANACCGGAGTTGTTGAAGGTCGTTAATAGAAACTNGCACCACTGGACCGGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATTGCGGCATTGCTTTTTTACTTCACGGAATATTTGGCCACGGTCGCtt                                              <  2:529262/298‑1 (MQ=255)                                                                                                                                                                                                                                gTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgc                                          >  1:465101/1‑299 (MQ=255)                                                                                                                                                                                                                                   aaTCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTTGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACTGAATATTTTGCCACGGTCGCTTTCGCGCCa                                      <  1:112841/300‑1 (MQ=255)                                                                                                                                                                                                                                   aaTCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCa                                      <  1:112877/300‑1 (MQ=255)                                                                                                                                                                                                                                   aaTCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTGGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCa                                      <  1:112828/300‑1 (MQ=255)                                                                                                                                                                                                                                   aaTCAGAAAGGAGAAAACACCTAGGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCa                                      <  1:277376/300‑1 (MQ=255)                                                                                                                                                                                                                                    aTCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCgcgc                                        <  2:302878/297‑1 (MQ=255)                                                                                                                                                                                                                                             gagaAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGNTGTGAAGAGAAACTGGCACCACTGGACCTGATNCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCtaata                            <  2:528911/300‑1 (MQ=255)                                                                                                                                                                                                                                                  aaCACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGAc                       <  1:297661/300‑1 (MQ=255)                                                                                                                                                                                                                                                  aaCACCTATGACGACCTACAATCGTAACCGTAACACAATTACCACTTGCAGCCGTGTTATGGTTAGCGGCACCGTTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAGGAc                       <  2:327379/300‑1 (MQ=255)                                                                                                                                                                                                                                                      ccTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGt                   >  1:685038/1‑300 (MQ=255)                                                                                                                                                                                                                                                        tATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACCGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCAGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCAATGGACCTGATTCGTCTAGGCATGAACTAAGCGTGTGAATGCAGCCGATGGCGGCATTGCTTTTTTACTTCAAGGAATATTTTGCCAAGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTa                  >  2:688707/1‑299 (MQ=255)                                                                                                                                                                                                                                                               ccctccGATCGTAACCGCAACGCCATTACCCCTGGCCTCCTTGTTCTGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTAGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTcc           >  2:467374/6‑299 (MQ=255)                                                                                                                                                                                                                                                               acCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTAGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGTGCTAATAAAGACAAGTACGTTTcc           >  2:36258/1‑299 (MQ=255)                                                                                                                                                                                                                                                                        cataaccgtaacGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTAGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCtt  >  1:706403/3‑299 (MQ=255)                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                          ATTTGCAGGAAATCAGCGAATCCGTGCGCCAGATCCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAA‑GATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTT  >  NC_000913/1628968‑1629528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑