Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,609,148	G→A	intergenic (‑126/+81)	yneE ← / ← uxaB	PF01062 family inner membrane protein YneE/tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,609,148	0	G	A	100.0%	158.5 / NA	47	intergenic (‑126/+81)	yneE/uxaB	PF01062 family inner membrane protein YneE/tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/20);  total (27/20)

GTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGT  >  NC_000913/1608857‑1609443                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                            gTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGc                                                                                                                                                                                                                                                                                                     >  1:660429/1‑300 (MQ=255) gTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCAAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGTGCTGCATGTGGCACCTGTTAATAATAAAGGc                                                                                                                                                                                                                                                                                                     >  2:7326/1‑300 (MQ=255)       gggTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAg                                                                                                                                                                                                                                                                                               <  1:548506/300‑1 (MQ=255)                    gCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAANTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACATGTTAATAATAAAGGCATATAGGATGTTGTAACt                                                                                                                                                                                                                                                                                   >  1:159846/1‑299 (MQ=255)                      ccAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAat                                                                                                                                                                                                                                                                                >  1:610168/1‑300 (MQ=255)                        aGATGCGTGTACCAGTGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTNGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAat                                                                                                                                                                                                                                                                                <  1:148102/298‑1 (MQ=255)                           tGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGt                                                                                                                                                                                                                                                                           >  2:383915/1‑300 (MQ=255)                            gCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTg                                                                                                                                                                                                                                                                          <  1:424381/300‑1 (MQ=255)                              gtgtACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAACTTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGaa                                                                                                                                                                                                                                                                        >  1:275963/1‑300 (MQ=255)                                 tACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACCACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAAATAATATGGTGAAATa                                                                                                                                                                                                                                                                     >  2:605913/1‑300 (MQ=255)                                 tACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATAAGGCGCAGCCAGTGTTGTTGTAGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATACAGGATGTTGTAACTAATATGGTGGAATa                                                                                                                                                                                                                                                                     >  2:329727/1‑300 (MQ=255)                                 tACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGCGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGGAATa                                                                                                                                                                                                                                                                     >  2:322813/1‑300 (MQ=255)                                           gcaTGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAAccc                                                                                                                                                                                                                                                             >  1:685058/1‑298 (MQ=255)                                                                          aTTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGttat                                                                                                                                                                                                                           >  1:569087/1‑300 (MQ=255)                                                                                     aGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACNAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACgg                                                                                                                                                                                                                <  2:524531/300‑1 (MQ=255)                                                                                           aGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTAc                                                                                                                                                                                                          >  2:160257/1‑300 (MQ=255)                                                                                                atCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCtt                                                                                                                                                                                                     >  2:688654/1‑300 (MQ=255)                                                                                                  cagggATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTc                                                                                                                                                                                                    >  1:196083/4‑299 (MQ=255)                                                                                                     ggATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCgcgc                                                                                                                                                                                                >  1:102294/1‑300 (MQ=255)                                                                                                          ataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATccc                                                                                                                                                                                           >  2:634282/1‑300 (MQ=255)                                                                                                          ataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATc                                                                                                                                                                                             >  1:39208/1‑298 (MQ=255)                                                                                                          ataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGAGCATccc                                                                                                                                                                                           >  2:634245/1‑300 (MQ=255)                                                                                                          ataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAANTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTAGCGCATcc                                                                                                                                                                                            >  2:502819/1‑299 (MQ=255)                                                                                                                                             cAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCAGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCAAGGTAA‑TTAGC‑AACCt                                                                                                                                                      >  2:202387/1‑300 (MQ=255)                                                                                                                                              aGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTNCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAATTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AAcc                                                                                                                                                       <  2:214677/298‑1 (MQ=255)                                                                                                                                               gtgtTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTCGC‑AACCTGc                                                                                                                                                    >  2:631951/1‑300 (MQ=255)                                                                                                                                                 gttgttgtGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTNCAAGCTTACAACATTAGCAGGCGCTGCATGTGNCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTc                                                                                                                                                  >  2:538189/1‑300 (MQ=255)                                                                                                                                                              aaCAATCATGGTTATCTTCATGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGc                                                                                                                                    <  1:634282/300‑1 (MQ=255)                                                                                                                                                              aaCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGc                                                                                                                                    <  1:634245/300‑1 (MQ=255)                                                                                                                                                               aCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTANAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGTTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCa                                                                                                                                   <  1:160257/300‑1 (MQ=255)                                                                                                                                                                              ttCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTcc                                                                                                                     <  1:7326/299‑1 (MQ=255)                                                                                                                                                                                  ggCGTGTAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTc                                                                                                                <  2:304645/300‑1 (MQ=255)                                                                                                                                                                                  ggCGTGGAAAAGTCGCNGCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCCGGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCGGTTAGCGNACCTGCTCGACCAGACCCNGGCCCTTGcttagc                                                                                                                         >  1:588566/1‑292 (MQ=255)                                                                                                                                                                                       gtaaaaGTCGC‑‑CCTATTAAACTTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGTTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGt                                                                                                           <  2:405785/298‑1 (MQ=255)                                                                                                                                                                                       ggAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGt                                                                                                           <  2:443679/300‑1 (MQ=255)                                                                                                                                                                                                       aTTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGcc                                                                                             >  1:349939/1‑300 (MQ=255)                                                                                                                                                                                                            aCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAAc                                                                                        <  1:346714/300‑1 (MQ=255)                                                                                                                                                                                                                          acaTTCTCAAGACGCTACGTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAg                                                                            <  1:80144/298‑1 (MQ=255)                                                                                                                                                                                                                                                              cTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACNTAc                                                                                                                                                                                                          >  2:515637/1‑139 (MQ=255)                                                                                                                                                                                                                                                              cTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTAc                                                                                                                                                                                                          <  1:515637/139‑1 (MQ=255)                                                                                                                                                                                                                                                                   aacacgaGCAGGCGCTACATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTTCGTCAGGTCCTGCTCCCATTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCagag                                  <  2:159846/293‑1 (MQ=255)                                                                                                                                                                                                                                                                   aacaTTAGCAGGCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGt                                 <  1:605913/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             ggCGCTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAAc                       <  2:610168/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gcgcTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAAc                       >  1:75303/1‑299 (MQ=255)                                                                                                                                                                                                                                                                              gcgcTGCATGTGGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCAAGAGTTGCTGATAAc                       >  1:76618/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                      tgtgGCACCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTccagcc              <  2:660429/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            aCCTGTTAATAATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAgtgt         <  1:383915/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  taataataaAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGt  <  2:257278/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                            GTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGC‑‑CCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTC‑AGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCA‑TTAGC‑AACCTGCTCGACCAGACC‑AGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGT  >  NC_000913/1608857‑1609443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑