Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,176,122	C→T	V232V (GTC→GTT)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,176,122	0	C	T	100.0%	143.3 / NA	40	V232V (GTC→GTT)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

GTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGC  >  NC_000913/1175856‑1176388                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                           gTCATCCTCGGCGAACAACTTGCCTCACAGCTAGTCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGTATGCCTCGCGTCTGATGCGTTATCCGGCATGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGgc                                                                                                                                                                                                                                           <  2:405046/300‑1 (MQ=255)           gCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGc                                                                                                                                                                                                                                 >  1:292716/1‑300 (MQ=255)                   cTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACt                                                                                                                                                                                                                           >  1:76954/1‑298 (MQ=255)                     tGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTggtg                                                                                                                                                                                                                       <  1:707381/300‑3 (MQ=255)                            caGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGt                                                                                                                                                                                                                >  1:164187/1‑300 (MQ=255)                                 aGGCGGTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAgg                                                                                                                                                                                                           <  1:629196/300‑1 (MQ=255)                                  ggCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGTGTGATCGTAAAGGc                                                                                                                                                                                                          >  2:494166/1‑300 (MQ=255)                                  ggCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGc                                                                                                                                                                                                          >  2:494229/1‑300 (MQ=255)                                            gcgGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTc                                                                                                                                                                                                 <  2:76954/299‑1 (MQ=255)                                                tGATCAAATCCGCGTGATGGTGCCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGc                                                                                                                                                                                            <  2:311318/300‑1 (MQ=255)                                                tGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGGGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGc                                                                                                                                                                                            <  1:395198/300‑1 (MQ=255)                                                 gATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACTCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACATTGAAGTCGATGGCTATGAAATGCTGTTGAATATTTAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGc                                                                                                                                                                                            <  2:9203/299‑1 (MQ=255)                                                                          cTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCNGGTGAATATTGAGGATGCCTCGCGTCTGATGCNTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATatgat                                                                                                                                                                    >  2:533576/1‑298 (MQ=255)                                                                                   agTTCACGCCGATGGTGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCCCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCTAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTActgc                                                                                                                                                         <  2:200184/300‑1 (MQ=255)                                                                                   agTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTActgc                                                                                                                                                         <  1:611431/300‑1 (MQ=255)                                                                                   aattCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTActgc                                                                                                                                                         <  2:164187/298‑1 (MQ=255)                                                                                    gTTCACTCCGATGGGGCGTAGTCCAAGCCAGCCTCTGTTCAATTTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAAGGCTGGTGAATAGTGAGGAGGCCCCGCTTCTGATGCTTTATCCTGCAGTCAATATTACCTGCTTGCATTTGTTGCTGGATGAGCCGCTGAAAGTTGACTCAGTAAGTTAGCAAAAACTGCCTGAAGGCAGCAAATTGCAGGACTGGCGTGACCTTAAAGGTGAGTTGTTCCAGGCCGGACGCATGGGAAAAAATATGATGGGTTTActgc                                                                                                                                                         <  2:610096/299‑1 (MQ=255)                                                                                    gTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTActgct                                                                                                                                                        >  1:76814/1‑300 (MQ=255)                                                                                             gATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTNTGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGcc                                                                                                                                                   <  1:157242/296‑1 (MQ=255)                                                                                                  ggCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAANGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATg                                                                                                                                                                                  >  2:226494/1‑260 (MQ=255)                                                                                                  ggCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATg                                                                                                                                                                                  <  1:226494/260‑1 (MQ=255)                                                                                                  ggCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGGTGGGTTTACTGCTGAGCCTGATTGt                                                                                                                                            >  2:73877/1‑298 (MQ=255)                                                                                                  ggCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTc                                                                                                                                           >  2:457504/1‑299 (MQ=255)                                                                                                                                tgATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATTAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAc                                                                                                            <  1:638411/300‑1 (MQ=255)                                                                                                                                                 gccAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATAGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCAGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGACGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAAt                                                                                            >  2:204052/1‑299 (MQ=255)                                                                                                                                                      ccgTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGaa                                                                                      >  1:105255/3‑300 (MQ=255)                                                                                                                                                                    gCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTa                                                                         >  1:220191/1‑299 (MQ=255)                                                                                                                                                                                 ggTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAAc                                                           >  1:277378/1‑300 (MQ=255)                                                                                                                                                                                             ggATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAc                                               <  1:678894/300‑1 (MQ=255)                                                                                                                                                                                               aTGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCTTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTc                                             <  1:17229/300‑1 (MQ=255)                                                                                                                                                                                               aTGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTc                                             <  1:17228/300‑1 (MQ=255)                                                                                                                                                                                                tGCCTAGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACtac                                            >  2:163161/1‑298 (MQ=255)                                                                                                                                                                                                  ccTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACNTTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCcgc                                          >  1:158811/1‑300 (MQ=255)                                                                                                                                                                                                         tCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAAt                                   >  1:37073/1‑300 (MQ=255)                                                                                                                                                                                                               gCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgat                             <  1:477330/300‑1 (MQ=255)                                                                                                                                                                                                                   tATCCGGCAGGCAATATNACCGGCTGGCGTTTGTGGNTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCNACAAATCATGATGGTc                         <  1:599811/300‑1 (MQ=255)                                                                                                                                                                                                                            ggcaATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGt                 >  2:671773/1‑299 (MQ=255)                                                                                                                                                                                                                                atatTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCNTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGNAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCaa             >  2:535982/1‑299 (MQ=255)                                                                                                                                                                                                                                  atTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAggg          <  1:535982/300‑1 (MQ=255)                                                                                                                                                                                                                                           tGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTTGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATTGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcgc  <  1:337847/299‑1 (MQ=255)                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                           GTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGC  >  NC_000913/1175856‑1176388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑