Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,077,657	C→T	R409H (CGC→CAC)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,077,657	0	C	T	100.0%	162.6 / NA	48	R409H (CGC→CAC)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/29);  total (19/29)

GGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTAC  >  NC_000913/1077370‑1077948                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                    ggTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATa                                                                                                                                                                                                                                                                                           <  1:240928/298‑1 (MQ=255) ggTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCAGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGNGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATaa                                                                                                                                                                                                                                                                                          >  1:139607/1‑299 (MQ=255)   tagtagTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCAAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGc                                                                                                                                                                                                                                                                                        >  2:703739/1‑299 (MQ=255)   tagtagTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGANTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGcc                                                                                                                                                                                                                                                                                       <  1:511154/300‑1 (MQ=255)   tagtagTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGAGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGc                                                                                                                                                                                                                                                                                        >  2:597021/1‑299 (MQ=255)        gtTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAAt                                                                                                                                                                                                                                                                                  <  2:250463/300‑1 (MQ=255)          tCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAATTGCGGGTAGATTAGGTTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCATGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGACCACCAACGGGCAGTGTTTTTGATAAGCCTGAAt                                                                                                                                                                                                                                                                                  <  2:250450/298‑1 (MQ=255)             gCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACaa                                                                                                                                                                                                                                                                             <  1:82661/300‑1 (MQ=255)                                        gCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGNTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGtt                                                                                                                                                                                                                                                  >  2:560273/1‑300 (MQ=255)                                          gTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTc                                                                                                                                                                                                                                                 <  1:241187/299‑1 (MQ=255)                                             tGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTACTTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATCACAAAAACGATGCCGTTTCAGCCTGCCAGTTCCGGc                                                                                                                                                                                                                                             >  2:211692/1‑300 (MQ=255)                                              gggCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCTAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCt                                                                                                                                                                                                                                            <  2:633764/300‑1 (MQ=255)                                                       gCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACaga                                                                                                                                                                                                                                   >  1:497339/1‑300 (MQ=255)                                                                 cTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCca                                                                                                                                                                                                                          >  1:409674/1‑299 (MQ=255)                                                                                                agcTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAATGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCg                                                                                                                                                                                                 <  2:656584/293‑1 (MQ=255)                                                                                                agcTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGAc                                                                                                                                                                                          <  1:38908/300‑1 (MQ=255)                                                                                                               tcgaaaTAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTACGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCGGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGt                                                                                                                                                                                <  2:64927/290‑1 (MQ=255)                                                                                                               gCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGCTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGATGGAGATCTCCAGGCGATCGGACTCTTCGGCGt                                                                                                                                                                                <  2:437438/295‑1 (MQ=255)                                                                                                                  agaTAAAAAACGTCGGTATACACCTTGCGGGTATAAACCGGACAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAACACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCAATCGGACTCTTCGGCGTCAATGtt                                                                                                                                                                         >  2:99182/1‑299 (MQ=255)                                                                                                                             ggcGGTATACACCTTGCGGTTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAatat                                                                                                                                                              <  2:472786/297‑1 (MQ=255)                                                                                                                                ggTATACACCTTGCGGNTATAAACCGGATAACCTTNAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATNTTGATACCAATATCGt                                                                                                                                                           <  1:597021/299‑1 (MQ=255)                                                                                                                                ggTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCATGAATAACAAAAACGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTACAGACGATCGGACTCTTCGGCGTCGATTTTGATACCAATATCGTa                                                                                                                                                          >  2:361212/1‑300 (MQ=255)                                                                                                                                ggTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGATCGTCCATCTGTGCACGCTTAATTTCACTATCCAAGTACGCGTCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGATGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCAATGCCGTTCCAGCCTGCCAGTTCTGGCTCGACACAGAGTTTTTCCAGCAGATCGAGAAAGATCTCCCGGCGATCGGACTCTTCGTCGTCAATGTTGATACCAatat                                                                                                                                                              >  2:107833/1‑296 (MQ=255)                                                                                                                                 gTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTAc                                                                                                                                                         >  1:612212/1‑300 (MQ=255)                                                                                                                                   ataCACCTTGCGGGTATAAACCGGATAACCTTCAATGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGTCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTg                                                                                                                                                       <  2:58927/300‑1 (MQ=255)                                                                                                                                        ccTTGCGGGTATAAACCGGATAAACTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCCGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAAGTAATCGATCACCAACGGGCAGTGTCTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTCTTTCAAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTAGGCGTCAATGTTGATACCAATCTCGTACTGAcga                                                                                                                                                   >  2:658488/1‑298 (MQ=255)                                                                                                                                                              aCCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCa                                                                                                                             >  1:669695/1‑299 (MQ=255)                                                                                                                                                                                         cTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGCTTTCAGACGCGGGTAAAGCTCTTCCATTAccc                                                                                                  >  1:218144/1‑299 (MQ=255)                                                                                                                                                                                                  aCTATCCCAGTACGCGCCTTTCACCCGGCGAATCATCAGACTGCGACGGCTGCGTGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACTGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGACCTCCAGTCGATCGGACTCTTCGGCGTCGATGTTGATACCAATATCGTACTGACGCACCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTg                                                                                        <  2:240802/300‑1 (MQ=255)                                                                                                                                                                                                  aCTATCCCAGTACGCGCCTTTCACCATGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTg                                                                                        <  2:648128/300‑1 (MQ=255)                                                                                                                                                                                                  aCTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTg                                                                                        <  1:336613/300‑1 (MQ=255)                                                                                                                                                                                                  aCTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTGCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTg                                                                                        <  1:56520/300‑1 (MQ=255)                                                                                                                                                                                                    tATCCCAGGACGCGCCTTTCACCAGGCGAATCATCATACGTCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGTGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTg                                                                                        <  2:320866/298‑1 (MQ=255)                                                                                                                                                                                                           gTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCt                                                                               >  1:444462/1‑300 (MQ=255)                                                                                                                                                                                                               gcgcCTTTCACCAGGCGGATCATCAGACGTCGACGGCTGCGGGTGTCGAGATCAATCAGGTAATCGATCACCAGCGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGAGCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATaa                                                                           <  2:602714/300‑1 (MQ=255)                                                                                                                                                                                                               gcgcCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATaa                                                                           <  2:602740/300‑1 (MQ=255)                                                                                                                                                                                                               gcgcCTTGCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATaa                                                                           <  2:349797/300‑1 (MQ=255)                                                                                                                                                                                                                 gcCTTTCACCAGTCGAATCATCAGACGGCGACGGCTGCGGTTGGCTAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAAcg                                                                         <  2:113430/300‑1 (MQ=255)                                                                                                                                                                                                                        aCCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGc                                                                  <  1:558418/300‑1 (MQ=255)                                                                                                                                                                                                                               gAATCATCAGATGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAgcgc                                                             <  1:243160/298‑1 (MQ=255)                                                                                                                                                                                                                                  tcatcaGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACa                                                        >  1:339332/1‑300 (MQ=255)                                                                                                                                                                                                                                  tcatcaGACGGCCACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAgcgc                                                             <  1:243044/295‑1 (MQ=255)                                                                                                                                                                                                                                                  gcTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGAAAGTTTGATTGAAAttcc                                        >  2:430456/1‑297 (MQ=255)                                                                                                                                                                                                                                                  gcTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCAAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                                        >  1:57531/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gaTCAATCAGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGa                          <  2:419291/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aGGTAATCGATCACCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGa                  >  1:686126/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    aCCAACGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGc       <  1:703739/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                        aCGGGCAGTGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTAc  <  1:560273/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                    GGTAGTAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGACGGCTGCGGGTGGCGAGATCAATCAGGTAATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTAC  >  NC_000913/1077370‑1077948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑