Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,599,487	C→T	A58T (GCT→ACT)	livJ ←	branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,599,487	0	C	T	100.0%	281.0 / NA	81	A58T (GCT→ACT)	livJ	branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/41);  total (40/41)

AGAATATATTTCGCCGCCGTCGGCCCCTGGTCGGAGTCCAGGCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGAT  >  NC_000913/3599192‑3599779                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      aGAATATATTTCGCCGCCGTCGGCCCCTGGTCGGAGTCCAGGCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTNTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGtt                                                                                                                                                                                                                                                                                                    >  2:561416/1‑299 (MQ=255) aGAATATATTTCGCCGCCGTCGGCCCCTGGTCGGAGTCCAGGCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACAGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTCTAGTGtt                                                                                                                                                                                                                                                                                                    >  2:209891/1‑299 (MQ=255)    atatatTTCGCCGCCGTCGGCCCCTGGTCGGAGTCCAGGCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGNTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGata                                                                                                                                                                                                                                                                                                <  2:554881/300‑1 (MQ=255)             gccgccTTCGGCCCCTGTTCGGAGTCCAGGCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAAc                                                                                                                                                                                                                                                                                        <  2:481237/299‑1 (MQ=255)                      ggCCCCTGGTCGGAGTCCAGGCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGctc                                                                                                                                                                                                                                                                               <  1:56756/299‑1 (MQ=255)                             ggTCGGAGTCCAGGCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGcc                                                                                                                                                                                                                                                                         >  1:260251/1‑298 (MQ=255)                                    gTCCAGGCCGGTGGTGNGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTCATGCCGCCTTTAGTGTNGATATCCGCAACCGCCTGNTCTGCGCCGG‑TAAACTc                                                                                                                                                                                                                                                               >  1:588428/1‑300 (MQ=255)                                    gTCCAGGCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTc                                                                                                                                                                                                                                                               >  1:343249/1‑300 (MQ=255)                                         ggCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑ACGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGt                                                                                                                                                                                                                                                          >  1:277763/1‑300 (MQ=255)                                         ggCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGt                                                                                                                                                                                                                                                          >  1:277930/1‑300 (MQ=255)                                         ggCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTAAGATTTGCAGTTTGTTGCCTTTAATGCCGACTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGt                                                                                                                                                                                                                                                          >  2:92317/1‑300 (MQ=255)                                         ggCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCATGTTTCGGGTCACAGGCATCGTCATATTTTAAGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGt                                                                                                                                                                                                                                                          >  1:277756/1‑300 (MQ=255)                                          gCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTc                                                                                                                                                                                                                                                         <  1:30663/300‑1 (MQ=255)                                          gCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCGGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTc                                                                                                                                                                                                                                                         <  1:31057/300‑1 (MQ=255)                                              gtggtgCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCCCAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAAACGCCTGCTCTGCGCAGG‑TAAACTCCTGGTCAcc                                                                                                                                                                                                                                                      >  2:109687/1‑299 (MQ=255)                                              gtggtgCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCAcc                                                                                                                                                                                                                                                      >  2:625909/1‑299 (MQ=255)                                                 gtgCGCAGGATCAGCTGNTAGCCACGGGCGGTCAGCNCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGCCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAANCTCCTGGTCACCGTAc                                                                                                                                                                                                                                                  <  1:602054/300‑1 (MQ=255)                                                 gtgCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTAc                                                                                                                                                                                                                                                  <  1:31898/300‑1 (MQ=255)                                                 gtgCGCAGGATCAGCTGATAACCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTAc                                                                                                                                                                                                                                                  <  1:655867/300‑1 (MQ=255)                                                       aGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTgcgc                                                                                                                                                                                                                                             <  1:695914/299‑1 (MQ=255)                                                        ggATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGACCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCa                                                                                                                                                                                                                                            >  2:68517/1‑299 (MQ=255)                                                        ggATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGNCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTCCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCa                                                                                                                                                                                                                                            >  1:137866/1‑299 (MQ=255)                                                        ggATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCAGCATTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCATGGTCACCGTACTgcgcca                                                                                                                                                                                                                                           >  2:68136/1‑298 (MQ=255)                                                              gCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAaccggac                                                                                                                                                                                                                                     >  1:660430/1‑300 (MQ=255)                                                                    aGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCg                                                                                                                                                                                                                                                                                      <  1:621215/246‑1 (MQ=255)                                                                    aGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCg                                                                                                                                                                                                                                                                                      >  2:621215/1‑246 (MQ=255)                                                                     gCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACAt                                                                                                                                                                                                                              <  1:665893/300‑1 (MQ=255)                                                                            gCGGTCAGCTCCTACGCGGTTGCCGCGTGGGTGATCATTAAAATTCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCNCATGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCGGCGCCGT‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGccc                                                                                                                                                                                                                       <  2:506673/300‑1 (MQ=255)                                                                               gTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGAGGTCAGACGCAGGCTGCGTTGATT‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACg                                                                                                                                                                                                                    <  2:612006/300‑1 (MQ=255)                                                                                  aGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGAc                                                                                                                                                                                                                  <  1:532022/299‑1 (MQ=255)                                                                                           gcgGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATGCGAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCNGGGTAAACTCCTGGTc                                                                                                                                                                                                                                                         <  1:588058/253‑1 (MQ=255)                                                                                              gTTGCCGCTGGGGTGAGCATTAAAATTCCGTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGGCCAATTACATATTTAATGCCGTCGTTAACGACTTTGTTCTCCACCGCAACCGTCTGTTTCGGGTCACAGGCAGCGTTATATTTTACGATTTGCAGTTTGTTGCCTTTAATTCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCACACg                                                                                                                                                                                                                    <  2:269202/285‑1 (MQ=255)                                                                                               ttGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCACCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAatat                                                                                                                                                                                                    <  1:46679/300‑1 (MQ=255)                                                                                                  ccGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATAtctt                                                                                                                                                                                                 >  1:661205/1‑300 (MQ=255)                                                                                                  ccGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCNCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATAtctt                                                                                                                                                                                                 >  1:514426/1‑300 (MQ=255)                                                                                                  ccGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAAGCATCGTCATATTTTACGCTTTGCAGTTTGTTGCCTTTAATGCAGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATAtctt                                                                                                                                                                                                 >  1:433399/1‑300 (MQ=255)                                                                                                                 ttAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCatat                                                                                                                                                                                  >  1:216129/1‑300 (MQ=255)                                                                                                                      aTGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTg                                                                                                                                                                             <  2:130342/300‑1 (MQ=255)                                                                                                                                       gctGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACa                                                                                                                                                            >  1:129078/3‑300 (MQ=255)                                                                                                                                          gTCAGACGCAGGCTGCGTTGATT‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATcc                                                                                                                                                         <  1:298090/300‑1 (MQ=255)                                                                                                                                          gTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACTACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATcc                                                                                                                                                         <  2:682795/300‑1 (MQ=255)                                                                                                                                          gTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACCTcc                                                                                                                                                         >  2:241600/1‑300 (MQ=255)                                                                                                                                                       tGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAATTTGGTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATATTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGc                                                                                                                                             <  1:237658/299‑1 (MQ=255)                                                                                                                                                             gatg‑aAGAACAGAGGTGACCAATCACATATTTAATGCCTTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTAccc                                                                                                                                      <  1:561416/300‑1 (MQ=255)                                                                                                                                                             gatg‑aAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTAccc                                                                                                                                      <  1:209891/300‑1 (MQ=255)                                                                                                                                                                    gaaCAGAGGTGACCAGTCACATATTTAATGCCGTCGTTAACGAATTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCGTATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCATTTAGTGTTGATCTCCGCAACCGCATGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACTGGACATTGCGCACACGACCGCGACTTTCATATCTTCTGCCAGAGCCATATTGCTGAGTGCCAGCGCGATACCTCCTGCCAGtcacacct                                                                                                                                           >  2:120107/1‑282 (MQ=255)                                                                                                                                                                    gaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTAt                                                                                                                                 >  1:91520/1‑299 (MQ=255)                                                                                                                                                                    gaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCAAGTAACGCTTTACCCTTTAt                                                                                                                                 >  2:492001/1‑299 (MQ=255)                                                                                                                                                                    gaaCAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCATGTTTAGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTa                                                                                                                                  >  2:391143/1‑298 (MQ=255)                                                                                                                                                                                  aaTCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACATGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGaa                                                                                                                   <  1:640218/299‑1 (MQ=255)                                                                                                                                                                                  aaTCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTgaga                                                                                                                    <  2:471100/298‑1 (MQ=255)                                                                                                                                                                                   aTCACATATTTAATGCNGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCNAGTAACGCTTTACCCTTTNTGTTCATCCTGAGAATc                                                                                                                 >  1:593586/1‑300 (MQ=255)                                                                                                                                                                                                       gtTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCGTATCTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATTCGCCACCGACTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGCACGCGGCTTTAATATCTTCTGCAAGAGCCATATTGCTGAATGCAAGCGCGGTACATCCTGCCCGTCACGCTTTa                                                                                                                                         >  2:136601/1‑256 (MQ=255)                                                                                                                                                                                                       gtTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTAGGGTAGCAGGCATCGTCATCTTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGACTTTAGTGTTGATATTCGCAACCGCTTGCTCTGCGCCGG‑TCAACTCCTGGTCACCGTCCTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAAGATCTTCTGCCAGAGCCATATTGCTGAATGCAAGCGCGATACATCCTGCAAGTAACGATTTAAACTTTATGTTTATCCTGAGAATCCAAATTATTATGGttattgc                                                                                             >  2:137202/1‑298 (MQ=255)                                                                                                                                                                                                        ttAACGACTTTGCTCGCCATCGCAACCGCCTTTTTCGGTTCACATGCAGCGTCATATTTTACGATTTGCAATTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACg                                                                                            <  2:343249/300‑1 (MQ=255)                                                                                                                                                                                                              aCTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGtgtt                                                                                      >  2:687327/1‑300 (MQ=255)                                                                                                                                                                                                                tttGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGAAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGtgttg                                                                                     <  1:527638/299‑1 (MQ=255)                                                                                                                                                                                                                 ttGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTATGGTTATTACGTGtgttgt                                                                                    >  2:633140/1‑299 (MQ=255)                                                                                                                                                                                                                   gTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGANACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTATGGTTATTACGTGTGTTGTGAt                                                                                 >  1:145936/1‑300 (MQ=255)                                                                                                                                                                                                                   gTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGANACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCACATTCTTCTGGTTATTACGTGTGTTGTGAt                                                                                 >  1:149068/1‑300 (MQ=255)                                                                                                                                                                                                                       gCCACCGCAACCGCCTGTTTCGGTTCACAGGCATCTTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGAtg                                                                                <  2:120648/297‑1 (MQ=255)                                                                                                                                                                                                                                       gTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTAt                                                             >  1:652741/1‑300 (MQ=255)                                                                                                                                                                                                                                        tttCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACg                                                                                            >  1:620416/1‑268 (MQ=255)                                                                                                                                                                                                                                        tttCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCCTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACg                                                                                            <  2:620416/268‑1 (MQ=255)                                                                                                                                                                                                                                                   aGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGc                                                 <  1:442928/300‑1 (MQ=255)                                                                                                                                                                                                                                                        tcttCATATTGTACGATTTGCGGTTTGTGGCCTTTAATGCCATCTTTAGTGTTGATATCCGCAACCGTCTGCTCTGCGCCGG‑TAAACTCCTGTTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGGGCCATATTGCTGAATGCCAGCGCGATATGTCCTGCCAGTAACGCTTTACCCTTTATGTTTGTCCTGAGAATCCCCATTCTTCTGGTTATTACGTTTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGAc                                            <  2:245500/297‑1 (MQ=255)                                                                                                                                                                                                                                                        tCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGAc                                            >  1:664945/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCAGGAc                                            <  1:192163/298‑1 (MQ=255)                                                                                                                                                                                                                                                               atTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGt                                     >  1:395474/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                ttgttgCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTTTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATActc                    <  1:109687/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                ttgttgCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATActc                    <  1:92317/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                ttgttgCCTTTAATGCCGCCTTTAGTGTTGATATCCACAACCGCCTGCTCTGCGCCGG‑TAAACTCCGGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTTTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATActc                    <  2:260251/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 tgttgCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATActct                   <  1:562654/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   ttgCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTTTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGc                 <  1:68136/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   ttgCCTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGc                 <  1:68517/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       cTTTAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTaaa             >  1:424332/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        tttAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTTTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATActct                   <  2:593586/293‑1 (MQ=255)                                                                                                                                                                                                                                                                                         ttAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAAc           <  2:567027/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          tAATGCCGCCTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACa          <  2:424332/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                cgccTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGCATGACAGCGCGATACATCCTGCCAGTAACGCTTTAACCTTTATGTTCATCCTGAGAATCCCCATTCTTATGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTCTTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCCTACTCTGCTAAAACATAccc     >  2:114812/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                  ccTTTAGTGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGAt  >  1:383493/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      AGAATATATTTCGCCGCCGTCGGCCCCTGGTCGGAGTCCAGGCCGGTGGTGCGCAGGATCAGCTGATAGCCACGGGCGGTCAGCTCCGGCGCGGTTGCCGCTGGGGTGATCATTAAAATGCCTTCGTCTTCGTAGATGTCAGACGCAGGCTGCGTTGATG‑AAGAACAGAGGTGACCAATCACATATTTAATGCCGTCGTTAACGACTTTGTTCGCCACCGCAACCGCCTGTTTCGGGTCACAGGCATCGTCATATTTTACGATTTGCAGTTTGTTGCCTTTAATGCCGCCTTTAGCGTTGATATCCGCAACCGCCTGCTCTGCGCCGG‑TAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGAT  >  NC_000913/3599192‑3599779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑