Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,465,502	G→A	E141K (GAG→AAG)	gspM →	Type II secretion system protein GspM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,465,502	0	G	A	100.0%	347.8 / NA	98	E141K (GAG→AAG)	gspM	Type II secretion system protein GspM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (53/45);  total (53/45)

CATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGC  >  NC_000913/3465211‑3465776                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                    cATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAANGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCAcc                                                                                                                                                                                                                                                                             >  2:560631/1‑300 (MQ=255)           aCGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGc                                                                                                                                                                                                                                                                      <  1:429586/297‑1 (MQ=255)                 aaaaaaTTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGNAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTcc                                                                                                                                                                                                                                                              <  1:510342/299‑1 (MQ=255)                     aaTTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAg                                                                                                                                                                                                                                                         <  2:157534/300‑1 (MQ=255)                       ttAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGTGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAggg                                                                                                                                                                                                                                                       <  2:175295/300‑1 (MQ=255)                       ttAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAggg                                                                                                                                                                                                                                                       <  2:175308/300‑1 (MQ=255)                       ttAAGAAGGACATCAAATGGATGCAGGATCAAGCGAACACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCCATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAggg                                                                                                                                                                                                                                                       <  2:175665/300‑1 (MQ=255)                              ctgcgagatgtgtataagagacagaGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGcaaca                                                                                                                                                                                                                                                >  1:702418/25‑300 (MQ=255)                                aCATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaa‑t                                                                                                                                                                                                                                             <  2:119603/300‑1 (MQ=255)                                 gggggggggggtggggggtggagggggtagccATGGTTTACTCGGTCGTCCTGCGCTTACGCAGCACGTCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGGCAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTAACTACGGCACAGGTAAAATACGGCATTGTTATTGAAGACCTTCCGTTTAATCTTGCCGGAAACGGAAAGATCACCCTAAGGCATTTGTCCTTCAGGGCGCAACaa‑ta                                                                                                                                                                                                                                            >  2:393173/31‑300 (MQ=255)                                 cATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCATCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCAGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaa‑ta                                                                                                                                                                                                                                            >  2:271015/1‑300 (MQ=255)                                  aTCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaa‑taa                                                                                                                                                                                                                                           <  1:360849/300‑1 (MQ=255)                                     aaaTGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCTCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGGCAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATAAGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGACCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATg                                                                                                                                                                                                                                         <  2:433917/299‑1 (MQ=255)                                           aTGCAGGATCAAGCGAGCACCCATGGTTTACTCGTTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGANAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGc                                                                                                                                                                                                                                  <  2:519157/300‑1 (MQ=255)                                           aTGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGc                                                                                                                                                                                                                                  >  1:2536/1‑300 (MQ=255)                                               agaaTCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTAc                                                                                                                                                                                                                               <  2:638269/296‑1 (MQ=255)                                               aGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTAc                                                                                                                                                                                                                               <  1:86640/299‑1 (MQ=255)                                               aGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTCCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTAc                                                                                                                                                                                                                               <  1:87413/299‑1 (MQ=255)                                                ggATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGCCTCCCGCTGGCTCACTACGGTACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAGAAGATCACCCTCAGGCATTTGTCCTTCATGGAGCAACAA‑TAATGACAATGCTAc                                                                                                                                                                                                                               <  1:87912/298‑1 (MQ=255)                                                  aTCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGcc                                                                                                                                                                                                                           <  1:642800/300‑1 (MQ=255)                                                              cccATGGTTTACTCGGTCATCCTGCGCTTACGCGGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCGTCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATATGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGCAGCGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAACGAATGACAATGCTACTGCAGCTCTTcc                                                                                                                                                                                                                   >  2:242337/1‑296 (MQ=255)                                                              cccATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCAGCTCTCCATTCt                                                                                                                                                                                                               >  1:281146/1‑300 (MQ=255)                                                              cccATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTAAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCt                                                                                                                                                                                                               >  1:281246/1‑300 (MQ=255)                                                              cccATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCt                                                                                                                                                                                                               >  1:348748/1‑300 (MQ=255)                                                              cacATGGTCTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTAGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGACACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATTACAATGCTACTGCAGCTCTTCATTCt                                                                                                                                                                                                               >  2:114312/3‑300 (MQ=255)                                                                  tGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTAAGGCATTTGTCCTTCAGGGAGCAACCA‑TAATGACAATGCTACTGCCGCTCTTCATTCtggtt                                                                                                                                                                                                           >  2:99720/1‑300 (MQ=255)                                                                   ggTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTATCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGTATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTAATGCCGCTATTCATTCtggtt                                                                                                                                                                                                           >  2:684710/1‑299 (MQ=255)                                                                           cGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTAtt                                                                                                                                                                                                  >  2:438100/1‑300 (MQ=255)                                                                             gTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGTTGGAAAACTGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGTTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGTAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGTGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGc                                                                                                                                                                                                <  2:305942/300‑1 (MQ=255)                                                                             gTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGc                                                                                                                                                                                                >  1:181919/1‑300 (MQ=255)                                                                             gTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATGCGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCCACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTAGTTGGTTTTAtt                                                                                                                                                                                                  >  2:37681/1‑298 (MQ=255)                                                                             gTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCACCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGc                                                                                                                                                                                                >  1:182086/1‑300 (MQ=255)                                                                                      cgcTTACGCAGCCCATCAAAAATATCCTTCTAGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACCACCGCTCTTAATTCTGGTTGGTTTTATTGCAGATTAttt                                                                                                                                                                                       >  2:208046/1‑300 (MQ=255)                                                                                      cgcTTACGCAGCCCATCAAAAATATCCTTATAGAGGAAGCAAAACGTGAGAATTTGGATATCAAGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCATCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATACAGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAC‑TAATGCCAATGCTACTGTCGCTCTTCATTCTGGTTGTTTTTATTGCGGATTAttt                                                                                                                                                                                       >  2:208040/1‑300 (MQ=255)                                                                                      cacTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATCTGGCTATCACGCTGGAAAACGGCACGGACAATACTATGACAATTCATCCTGTAACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGAACAGGTAACATACGGTATTGTTATTGACGACCTTCAGTTTACTCTTGCCGGAACCGAAAAGATCAACCTCAGGCATTTGTACTTCAGGGAGCAACaa‑taat                                                                                                                                                                                                                                          >  2:676827/3‑249 (MQ=255)                                                                                        cTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTAtttt                                                                                                                                                                                      >  1:111889/1‑299 (MQ=255)                                                                                                            tgtCCTTCTTGAGGAAGCAAAATGTGGGAATTTGGCTATCACGCTGGAAAACGGCCCGGACGATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCATGGAGCAACAA‑TAATGACAATGCTATTGCCGTTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGTCATCGCCTATCAc                                                                                                                                                                 <  2:472537/298‑1 (MQ=255)                                                                                                            tatCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACTCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCAc                                                                                                                                                                 <  2:687109/300‑1 (MQ=255)                                                                                                            tatCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCAc                                                                                                                                                                 <  1:271015/300‑1 (MQ=255)                                                                                                             atCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCATGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCAc                                                                                                                                                                 <  2:472438/299‑1 (MQ=255)                                                                                                             atCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCAc                                                                                                                                                                 <  2:472428/299‑1 (MQ=255)                                                                                                                 ttCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACctct                                                                                                                                                             <  1:497110/299‑1 (MQ=255)                                                                                                                 ttCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTGCAGGGAGCAACAA‑TAATGACAATGCTACTGCAGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCAATCGCCTATCAACtctc                                                                                                                                                            >  1:91906/1‑300 (MQ=255)                                                                                                                       aGGAAGCAAAACTTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGt                                                                                                                                                       <  1:90735/299‑1 (MQ=255)                                                                                                                       aGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGtt                                                                                                                                                      >  2:590854/1‑300 (MQ=255)                                                                                                                              aaaaCGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGAt                                                                                                                                               <  2:673240/300‑1 (MQ=255)                                                                                                                                             ggCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACg                                                                                                                                <  1:187979/300‑1 (MQ=255)                                                                                                                                                tATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGtt                                                                                                                              <  2:438989/299‑1 (MQ=255)                                                                                                                                                                     ggACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCAttt                                                                                                         <  1:550347/299‑1 (MQ=255)                                                                                                                                                                     ggACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTc                                                                                                        >  1:204009/1‑300 (MQ=255)                                                                                                                                                                        cAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTACCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTAATGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCa                                                                                                       >  2:700772/1‑298 (MQ=255)                                                                                                                                                                          aTACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCa                                                                                                   >  2:648969/1‑300 (MQ=255)                                                                                                                                                                               ctGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTGTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCaaaaaa                                                                                              >  2:620429/1‑300 (MQ=255)                                                                                                                                                                               ctGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTAAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCaaaaaa                                                                                              >  1:63237/1‑300 (MQ=255)                                                                                                                                                                                     aTTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAatat                                                                                         >  1:685341/1‑299 (MQ=255)                                                                                                                                                                                             tGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCAt                                                                                <  1:55772/300‑1 (MQ=255)                                                                                                                                                                                              gTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGTATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAACATGCCTGGCAt                                                                                >  2:24161/1‑299 (MQ=255)                                                                                                                                                                                                   aGCTCCCCTCGAAAACGTCTCTCGCTGGCTCACTACGGCACAGTGAACATACGGTATTGTTATTGAAGACCTTCAGGTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATAc                                                                           <  2:281246/299‑1 (MQ=255)                                                                                                                                                                                                   aGCTCCCCTCGAAAACGTCTCCCTCTTGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATAc                                                                           <  2:131826/299‑1 (MQ=255)                                                                                                                                                                                                   aGCTCCCCTCGAAAACGTCTCCCGCTTGCTCACTACGGCACAGGTAACATACGGTATTGTTATTTAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATAc                                                                           <  2:34692/299‑1 (MQ=255)                                                                                                                                                                                                   aGCTCCCCTCGAAAACGTCTCCCGCTTGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTCCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATAc                                                                           <  2:281146/299‑1 (MQ=255)                                                                                                                                                                                                   aGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATAc                                                                           <  1:220832/299‑1 (MQ=255)                                                                                                                                                                                                   aGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATAc                                                                           <  2:161772/299‑1 (MQ=255)                                                                                                                                                                                                   aGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTNTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATAc                                                                           <  1:508185/299‑1 (MQ=255)                                                                                                                                                                                                       ccccTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaa‑taa                                                                                                                                                                                                                                           <  1:28617/135‑1 (MQ=255)                                                                                                                                                                                                       ccccTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaa‑taa                                                                                                                                                                                                                                           >  2:28635/1‑135 (MQ=255)                                                                                                                                                                                                       ccccTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaa‑taa                                                                                                                                                                                                                                           <  1:28635/135‑1 (MQ=255)                                                                                                                                                                                                       ccccTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaa‑taa                                                                                                                                                                                                                                           >  2:28617/1‑135 (MQ=255)                                                                                                                                                                                                             gAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTg                                                                 <  1:336853/299‑1 (MQ=255)                                                                                                                                                                                                                aaCGTCTCCTACTTGCTCACTACTGCACAGTTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAAATCACCCTCAGGCATTTGTCCTACAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGTTTGGTTTTATTGCAGATTACTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGagat                                                             <  2:91906/300‑4 (MQ=255)                                                                                                                                                                                                                          gCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTgtgt                                                      <  1:560631/297‑1 (MQ=255)                                                                                                                                                                                                                          gCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGCTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTgtgt                                                      <  1:210408/297‑1 (MQ=255)                                                                                                                                                                                                                              gCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgctgct                                               >  2:452757/1‑300 (MQ=255)                                                                                                                                                                                                                              gCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgctgct                                               >  1:466986/1‑300 (MQ=255)                                                                                                                                                                                                                              gCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGATCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgctgct                                               >  1:467037/1‑300 (MQ=255)                                                                                                                                                                                                                                tCACTACGGCACAGGTAACATACGGTATTGTTATTTAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCATGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgctgctg                                              <  2:429292/299‑1 (MQ=255)                                                                                                                                                                                                                                 cACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTgctgctgct                                            >  2:259085/1‑300 (MQ=255)                                                                                                                                                                                                                                 cACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCATGATACTTTGTGTTgctgctgct                                            >  2:259195/1‑300 (MQ=255)                                                                                                                                                                                                                                   cTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCATGATACTTTGTGTTGCTGCTGCTAt                                          >  1:637545/1‑300 (MQ=255)                                                                                                                                                                                                                                        gCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCt                                     >  2:473723/1‑300 (MQ=255)                                                                                                                                                                                                                                        gCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCt                                     >  1:529362/1‑300 (MQ=255)                                                                                                                                                                                                                                            aGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCTGAAACGAAAAGACCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑GAATGACAATGCTACTGCCGCTCGTCATTCTGGTTGGTTTTATTGCAGATGATGTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTgcgc                                 <  2:62349/300‑1 (MQ=255)                                                                                                                                                                                                                                             ggTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCCTGATACAGTGCACCTGATACTTTGTGTTGCTGCTGCTATCGCCTgcgc                                 >  2:323872/1‑299 (MQ=255)                                                                                                                                                                                                                                             ggTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTTCTATCGCCTGCGcc                                >  1:582980/1‑300 (MQ=255)                                                                                                                                                                                                                                             ggTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTCTCGCCTGCGcc                                >  1:670948/1‑300 (MQ=255)                                                                                                                                                                                                                                             ggTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGcc                                >  1:635290/1‑300 (MQ=255)                                                                                                                                                                                                                                             ggTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCATGCGcc                                >  1:353127/1‑300 (MQ=255)                                                                                                                                                                                                                                             ggTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCAATCGCCTATCACCTCTCGCAGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATCGGTTCATTTCAGGCAAAAAACATATACCTGGCATGATACAGTGCCCCTGCTACTTTGTGTTGCTGCTGCTATCGCCt                                     >  2:324434/1‑295 (MQ=255)                                                                                                                                                                                                                                                 aCATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTTGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCt                              >  1:73417/1‑298 (MQ=255)                                                                                                                                                                                                                                                 aCATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTTGTTGGTTTTATTGCAGATCATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCATGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCt                              >  1:74475/1‑298 (MQ=255)                                                                                                                                                                                                                                                    tACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCAc                         <  1:700772/300‑1 (MQ=255)                                                                                                                                                                                                                                                      cGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGNCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCt                       >  1:515520/1‑300 (MQ=255)                                                                                                                                                                                                                                                      cGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCt                       >  1:644477/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCttt                     >  1:226514/1‑300 (MQ=255)                                                                                                                                                                                                                                                              ttATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGcc               >  1:55722/1‑300 (MQ=255)                                                                                                                                                                                                                                                                aTTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCa             >  1:671760/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       aCCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGAc      <  1:372508/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           tCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGgc  >  1:433091/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                    CATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAA‑TAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGC  >  NC_000913/3465211‑3465776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑