Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,353,360	C→T	H238H (CAC→CAT)	glpA →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,353,360	0	C	T	100.0%	161.0 / NA	45	H238H (CAC→CAT)	glpA	anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

GGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGT  >  NC_000913/2353066‑2353647                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                ggTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCACTTCATGCACCTGTCGTGGTTAATGCAGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCAGGCGAAAGGATCGATGCTGATCATGGATCATCGCAt                                                                                                                                                                                                                                                                                            >  2:335693/1‑300 (MQ=255)             ggATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCANGCCCTTCATGCACCTGTCGTGGTTAATGCCGCNGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCAt                                                                                                                                                                                                                                                                                  <  2:520282/298‑1 (MQ=255)             ggATGGCACCGGTGATCCGTTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGGGCCGTTATCCTTACCGCTCATGAAGTCACTGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCTGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCAt                                                                                                                                                                                                                                                                                  <  2:177573/298‑1 (MQ=255)                           aTCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCAGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTAACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGCTCCTGGATCATCGCATTAACCAGCAGGTGATCAACCGCTGcc                                                                                                                                                                                                                                                                  >  2:436739/1‑300 (MQ=255)                                                  aaCATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCAGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCAGATAtt                                                                                                                                                                                                                                           >  2:237711/1‑300 (MQ=255)                                                         tGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTNCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGc                                                                                                                                                                                                                                    <  1:138500/300‑1 (MQ=255)                                                                    gAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATAcc                                                                                                                                                                                                                         >  1:69683/1‑300 (MQ=255)                                                                              ccGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCGTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCAGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGa                                                                                                                                                                                                               >  2:597405/1‑300 (MQ=255)                                                                              ccGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTAACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCAGGCGAAAGGATCGATGCTGATCATGGATCATCGCATTAACCAGTATGTGATCAACCGCTGCCGTAAACCTTCCGAAGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGa                                                                                                                                                                                                               >  2:275927/1‑300 (MQ=255)                                                                                                     gTCACGGGGCTGATTAGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGTCGAAACTCAGGCCCTTCATGCACCTTTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTAtt                                                                                                                                                                                        <  1:416020/300‑1 (MQ=255)                                                                                                           gggCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCNATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTAc                                                                                                                                                                                  >  2:480552/1‑300 (MQ=255)                                                                                                               tGATTCGTGAAGGCTCGACGGTGTGCGGTGTTCGGGTACGTAACCAGCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAAc                                                                                                                                                                               <  2:394672/299‑1 (MQ=255)                                                                                                               tGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAAc                                                                                                                                                                               <  1:237711/299‑1 (MQ=255)                                                                                                                        aaGGCGCTACGGTGTGCGGTGGTCGTGTACGTAACCATCTCACCGGCGAAACGCATGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACg                                                                                                                                                                     <  2:277784/300‑1 (MQ=255)                                                                                                                                  ggTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGt                                                                                                                                                           >  2:676185/1‑300 (MQ=255)                                                                                                                                  ggTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGt                                                                                                                                                           >  1:652596/1‑300 (MQ=255)                                                                                                                                          gtgtTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACTATAATCTAGTGACGGCa                                                                                                                                                    >  1:678409/1‑299 (MQ=255)                                                                                                                                          gtgtTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTATTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCa                                                                                                                                                    >  1:589232/1‑299 (MQ=255)                                                                                                                                             ttCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCAGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCAGATGTTCTGGTGCCTGGTGACACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGAAGATAATCGAGTGACGGCaga                                                                                                                                                  >  2:330215/1‑298 (MQ=255)                                                                                                                                             ttCGTGGACGTAACCATCTCACCGTCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCACTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGNTCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCagaag                                                                                                                                                <  2:211031/300‑1 (MQ=255)                                                                                                                                              tCGTGTACGTAACCATCTCACCTGCGAAACTCAGGCCCTTCATGCACCTGTCTTGGTTAATGCCTCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTTCCGGCGAAAGGATCGCTGCTGATCATGGAGCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCagaag                                                                                                                                                <  2:239954/299‑1 (MQ=255)                                                                                                                                              tCGTGTACGTAACCATCTCACCGTCGAAACGCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTTGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGAGCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGGAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACTACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCagaag                                                                                                                                                <  2:198394/299‑1 (MQ=255)                                                                                                                                                   tACGTAACCATCTAACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCAGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGGGGtt                                                                                                                                           >  2:63362/1‑299 (MQ=255)                                                                                                                                                                        aaaCTCAGGCCCTTCATGCGCCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACCTTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACAATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAgggg                                                                                                                     <  2:44845/300‑1 (MQ=255)                                                                                                                                                                              aGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAAc                                                                                                               <  1:635915/300‑1 (MQ=255)                                                                                                                                                                                    ttCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGccc                                                                                                         >  1:387224/1‑300 (MQ=255)                                                                                                                                                                                              tGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCTTGATgg                                                                                                <  1:190600/299‑1 (MQ=255)                                                                                                                                                                                              tGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATgg                                                                                                <  1:190459/299‑1 (MQ=255)                                                                                                                                                                                               gTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGc                                                                                               >  1:87036/1‑299 (MQ=255)                                                                                                                                                                                               gTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCATGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGc                                                                                               >  1:197377/1‑299 (MQ=255)                                                                                                                                                                                                              gCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCAtttt                                                                                >  1:424279/1‑299 (MQ=255)                                                                                                                                                                                                                   gATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGc                                                                          >  1:623761/1‑300 (MQ=255)                                                                                                                                                                                                                          gggCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCACGTGATGGCGAAAACGCGCATTTTGCGGGCATATTCt                                                                   >  2:108064/1‑300 (MQ=255)                                                                                                                                                                                                                              aaCACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCg                                                               <  1:163806/300‑1 (MQ=255)                                                                                                                                                                                                                              aaCACATTGCCGAATATGCCGATCTGCACATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCg                                                               <  2:651296/300‑1 (MQ=255)                                                                                                                                                                                                                                             atGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGtt                                                 >  1:208143/1‑299 (MQ=255)                                                                                                                                                                                                                                                                          gCGAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACg                       <  1:635518/296‑1 (MQ=255)                                                                                                                                                                                                                                                                            gAAAGGATCGCTGCTGATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACg                  <  1:593258/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ctgctgATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATGTTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGTGGACGTAACGTCAGCCGtcgc       >  2:53252/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                      ctgctgATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGc       >  1:464847/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      ctgctgATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTAGCTGATTGGTACCAACTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCACTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGc       >  2:53592/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      ctgccgATCATGGATCATCGCATTAAACAGCATGTGATCAACCACCGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTCCAACGAGATTGACGATACTCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCACCCGTGGTGGCGACAACGCGCAtctt                                                                                >  2:53261/1‑224 (MQ=255)                                                                                                                                                                                                                                                                                       tgctgATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGc       <  2:589232/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACNACCTCTTTACGTATTGATTACAACGAGATTGANGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGt  >  2:506738/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           gATCATGGATCATCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGt  >  1:288821/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                GGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGTTCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGCAACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATTAACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGGTACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTGAAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGCGATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGT  >  NC_000913/2353066‑2353647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑