Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,028,651	G→A	A239V (GCG→GTG)	yedI ←	DUF808 domain‑containing inner membrane protein YedI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,028,651	0	G	A	100.0%	240.1 / NA	65	A239V (GCG→GTG)	yedI	DUF808 domain‑containing inner membrane protein YedI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/30);  total (35/30)

AACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCTT  >  NC_000913/2028356‑2028946                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        aaCCATAAAGATCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGAGGGCGAATTTTGCAGAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTNCCAGCACCACGATACCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGGAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAg                                                                                                                                                                                                                                                                                                     <  2:208873/300‑1 (MQ=255)   ccATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGc                                                                                                                                                                                                                                                                                                    >  1:614510/1‑299 (MQ=255)     aTAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCg                                                                                                                                                                                                                                                                                                   <  2:415985/298‑1 (MQ=255)         aGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGa                                                                                                                                                                                                                                                                                             >  1:157255/1‑300 (MQ=255)                  tCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGGGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGAt                                                                                                                                                                                                                                                                                     <  2:239023/299‑1 (MQ=255)                                  aGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCa                                                                                                                                                                                                                                                                    >  2:613173/1‑300 (MQ=255)                                                     aGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAAc                                                                                                                                                                                                                                                 <  1:211659/300‑1 (MQ=255)                                                               ggCGAATTTTGCACAATAACCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTAcc                                                                                                                                                                                                                                         <  2:109781/298‑1 (MQ=255)                                                                   aaTTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCGACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCTAAATNTTCAATGGCGTGATGCAGCGGCGCAATTCCATNTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGc                                                                                                                                                                                                                                   <  2:510591/300‑1 (MQ=255)                                                                    aTTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc                                                                                                                                                                                                                                  <  1:4259/300‑1 (MQ=255)                                                                        tGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCa                                                                                                                                                                                                                              <  1:48471/300‑1 (MQ=255)                                                                                    aGTGAGAGTAGTAATGGGCCTGACCGCGCATTTGATTGACGGCTTTCACTCCCAGCACCACGGTGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCNGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGTGCGCGATAATCAATAAccc                                                                                                                                                                                                                                               <  2:210228/271‑1 (MQ=255)                                                                                     gTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCt                                                                                                                                                                                                                    >  1:252592/1‑297 (MQ=255)                                                                                         gAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAtttt                                                                                                                                                                                                             >  1:265763/1‑300 (MQ=255)                                                                                           gTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc                                                                                                                                                                                                                                  <  2:666448/277‑1 (MQ=255)                                                                                           gTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGcc                                                                                                                                                                                                                                  >  1:666448/1‑277 (MQ=255)                                                                                           gTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTggtagtgac                                                                                                                                                                                                           <  2:649042/300‑8 (MQ=255)                                                                                           gTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTc                                                                                                                                                                                                           >  1:225613/1‑300 (MQ=255)                                                                                           gTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCAACGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTc                                                                                                                                                                                                           >  1:646008/1‑300 (MQ=255)                                                                                               tAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCgcc                                                                                                                                                                                                       >  2:452381/1‑300 (MQ=255)                                                                                                        tGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGGTGCCGCCGATGATAAATCCAAGAATAAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTGTCATCAGCCAGGGCGCGATAATCAATAACCCTTGACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATAc                                                                                                                                                                                              <  2:1598/300‑1 (MQ=255)                                                                                                        tGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACNACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATAc                                                                                                                                                                                              <  2:489994/300‑1 (MQ=255)                                                                                                        tGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCAACAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATAc                                                                                                                                                                                              >  2:246232/1‑300 (MQ=255)                                                                                                          aCCGCGCATTTTCTCTACGGTTTTCACTCCCAACACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACATTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATAccc                                                                                                                                                                                            <  2:394253/300‑1 (MQ=255)                                                                                                               gcATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGt                                                                                                                                                                                       <  1:406317/300‑1 (MQ=255)                                                                                                                             gCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAAc                                                                                                                                                                          >  1:182618/1‑299 (MQ=255)                                                                                                                                    cTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATaaacc                                                                                                                                                                  >  2:557553/1‑297 (MQ=255)                                                                                                                                        cAGCACCACGATGCCGNCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCNATACCCCAGGTCATCAATNTTAACGATAACCCCTAc                                                                                                                                                              >  1:577387/1‑300 (MQ=255)                                                                                                                                         aGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGGAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCCGTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTAcc                                                                                                                                                             <  1:535593/300‑1 (MQ=255)                                                                                                                                                       gccgATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAAccc                                                                                                                                                                  <  2:427813/281‑1 (MQ=255)                                                                                                                                                       gccgATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACg                                                                                                                                                 <  2:393136/298‑1 (MQ=255)                                                                                                                                                              agaagtCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGTCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTc                                                                                                                                        <  2:67769/295‑1 (MQ=255)                                                                                                                                                                      aaGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTAcc                                                                                                                                  <  1:365709/298‑1 (MQ=255)                                                                                                                                                                      aaGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTAcc                                                                                                                                  <  2:182618/298‑1 (MQ=255)                                                                                                                                                                        gAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACTAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAgcg                                                                                                                              <  1:233059/300‑1 (MQ=255)                                                                                                                                                                              gATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCACTACCAGACCGTAGACGCCAACGGTCACTACCAGTGCGATGc                                                                                                                        >  2:36983/1‑300 (MQ=255)                                                                                                                                                                                          tcgttAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGTAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATNAATAACCCTTTACCTAATGCCTGCATCAGCGCNCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagc                                                                                                                <  2:519974/292‑1 (MQ=255)                                                                                                                                                                                             gTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcaccagca                                                                                                         >  1:653707/1‑300 (MQ=255)                                                                                                                                                                                              tAATATCATCGCCACTACTGCACTTTGCTGCCCTGCTAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcaccagcac                                                                                                        <  2:189548/300‑1 (MQ=255)                                                                                                                                                                                               aaTATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCNCAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcaccagcacc                                                                                                       <  1:136368/300‑1 (MQ=255)                                                                                                                                                                                                        gCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGc                                                                                              <  1:246232/300‑1 (MQ=255)                                                                                                                                                                                                         ccACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCCATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCATGAAAGCACCAGCACCTGATTAAGCa                                                                                             >  2:169781/1‑300 (MQ=255)                                                                                                                                                                                                            ctactGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCAATACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAAc                                                                                           >  2:346318/1‑299 (MQ=255)                                                                                                                                                                                                            ctactGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCACACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCaa                                                                                            >  2:30395/1‑298 (MQ=255)                                                                                                                                                                                                                                gCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCAACAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCAACAATcccc                                                                      >  1:548599/1‑300 (MQ=255)                                                                                                                                                                                                                                     aTGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGt                                                                 >  1:117712/1‑300 (MQ=255)                                                                                                                                                                                                                                     aTGTTCAATGGCGTGATGCAGCGGCGCAATTCCACGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCCTCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCCGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAACCTTAACGATAACCCCTACAAGACCGTAGAAGCCCACAGTCACTACCAGCGCGATGCCTGAAAGCACCAGCATCTGATTAAGCAACGGCGTTTCGGCCATAATCCCAAGCGt                                                                 >  1:117723/1‑300 (MQ=255)                                                                                                                                                                                                                                     aTGTTCAAGGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGTTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGt                                                                 <  2:157255/300‑1 (MQ=255)                                                                                                                                                                                                                                       gTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCCGCGTGa                                                               >  1:396708/1‑300 (MQ=255)                                                                                                                                                                                                                                       gTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTCCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCAACTGATTAAGCAACGGCGCTTCGGCAACAATCCCCAGCGTGa                                                               >  1:396717/1‑300 (MQ=255)                                                                                                                                                                                                                                              ggCGTGATGCAGCGGCNCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCANCGGCGCTTCGGCCACAATNCCCAGCGTGATGGcgac                                                        >  1:567343/1‑300 (MQ=255)                                                                                                                                                                                                                                              ggCGTGATGCAGCGGCNCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCATACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCANCGGCGCTTCGGCCACAATNCCCAGCGTGATGGcgac                                                        >  1:567313/1‑300 (MQ=255)                                                                                                                                                                                                                                               gCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGcgac                                                        >  1:474576/1‑299 (MQ=255)                                                                                                                                                                                                                                               gCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCAACAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACATGATTAAGCAACGGCGCTTCGGCCACAATCCCCAACGCGATGGcga                                                         >  2:116871/1‑298 (MQ=255)                                                                                                                                                                                                                                                            gcgcAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACa                                          >  1:525117/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gcgcAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAACGTGATGGCGACGATTTCCGCAGACa                                          >  2:449900/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 aTTCCATGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCACAGCGTGATGGCGACGATTTCCGCAGACAAtata                                     >  1:280816/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   tCCATGTACCACAATCCCACCGCCGACGAGGAACATCAACAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTAGGCCACAATCCCCAGCGTGATGGCGACGATTCCCGCAGACAATATaaa                                   >  2:363653/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      aTGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAAt                                 <  1:100553/299‑1 (MQ=255)                                                                                                                                                                                                                                                                      aTGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATc                                >  1:615661/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      aTGTACCACAATCCCACCGCCGACGAGGAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAACCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAAt                                 <  1:613173/299‑1 (MQ=255)                                                                                                                                                                                                                                                                              caATCCCACCGCCGACGAGGAACATCAACAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCAAGCGTGATGGTGACGATTTCCGCAGACAATATAAAATCGGTACGaa                        >  2:10525/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 gAACATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTc     >  1:530310/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  aaCATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTcc    <  2:614510/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    cATCACCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCtt  >  2:427638/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        AACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATGTACCACAATCCCACCGCCGACGAGGAACATCGCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCTT  >  NC_000913/2028356‑2028946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑